Roles of Tbx22 during early stage of secondary palate development.

• Project/Area Number: 19570213
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kawasaki Medical School
• Principal Investigator: WADA Naoyuki Kawasaki Medical School, 医学部, 助教 (50267449)
• Co-Investigator(Kenkyū-buntansha): NOHNO Tsutomu 川崎医科大学, 医学部, 教授 (20098619) ; NISHIMATSU Shin-ichiro 川崎医科大学, 医学部, 講師 (20222185)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 顎顔面形成 / 二次口蓋 / 舌 / Tbx22 / BMP / 顎顔面発生 / 口蓋板 / 舌形成 / 遺伝子発現抑制 / Noggin

Research Abstract

In this project, we focused on the developmental process of the secondary palate and tongue in chick embryo, and analyzed the expression and function of Tbx22, which is involved in X-linked cleft palate and ankyloglossia in human. Expression of Tbx22 is observed in the oral side of the maxillary process where the palatal shelf is emerged later, and in the bottom region of the tongue. The expression is regulated by BMP signaling. Misexpression of Tbx22 suppressed proliferation of cells in the facial processes, and inhibited osteogenesis of the cells, suggesting the suppressive role of Tbx22 on the formation of the secondary palate and tongue.

Research Products

• [Journal Article] The apical ectodermal ridge (AER) can be re-induced by wounding, wnt-2b, and fgf-10 in the chicken limb bud. (2010)
  • Author(s): Satoh, A., Makanae, A., Wada., N.
  • Journal Title: Dev Biol 342 Pages: 157-168
• [Journal Article] Essential mesenchymal role of small GTPase Rac1 in interdigital programmed cell death during limb development. (2009)
  • Author(s): Suzuki, D., Yamada, A., Amano, T., Yasuhara, R., Kimura, A., Sakahara, M., Tsumaki, N., Takeda, S., Tamura, M., Nakamura, M., Wada, N., Nohno, T., Shiroishi, T., Aiba, A., Kamijo, R.
  • Journal Title: Dev. Biol. 335 Pages: 396-406
• [Journal Article] 17β-estradiol inhibits chondrogenesis in the skull development of zebrafish embryos. (2009)
  • Author(s): Fushimi, S., Wada, N., Nohno, T., Tomita, M., Saijoh, K., Sunami, S., Katsuyama, H.
  • Journal Title: Aqua. Toxicol. 95 Pages: 292-298
• [Journal Article] Essential mesenchymal role of small GTPase Rac1 in interdigital programmed cell death during limb development (2009)
  • Author(s): Suzuki, Dai.
  • Journal Title: Developmental Biology 335 Pages: 396-406
  • Peer Reviewed
• [Journal Article] 17b-estradiol inhibits chondrogenesis in the skull development of zebrafish embryos (2009)
  • Author(s): Fushimi, Shigeko.
  • Journal Title: Aquatic Toxicology 95 Pages: 292-298
  • Peer Reviewed
• [Journal Article] A Wnt3a variant participates in chick apical ectodermal ridge formation:Distinct biological activities of Wnt3a splice variants in chick limbdevelopment (2007)
  • Author(s): Narita, T., Nishimatsu, S.-I., Wada, N., Nohno, T.
  • Journal Title: Developmental Dynamics 49 Pages: 493-501
  • Peer Reviewed
• [Journal Article] Nuclear localization of beta-catenin involved in precancerous change inoral leukoplakia (2007)
  • Author(s): Ishida, K., Ito, S., Wada, N., Deguchi, H., Hata, T., Hosoda, M., Nohno, T.
  • Journal Title: Molecular Cancer 9 Pages: 62-68
  • Peer Reviewed
• [Presentation] 索前軟骨頭蓋は, 異なる神経堤細胞群に由来する二つの構造から構成される (2010)
  • Author(s): 和田直之
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-03-28
• [Presentation] アフリカツメガエル口腔原基におけるTbx22の発現 (2009)
  • Author(s): 和田直之
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-11
• [Presentation] ニワトリ胚口腔形成過程におけるTbx22の発現とその調節 (2009)
  • Author(s): 和田直之
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡市
  • Year and Date: 2009-09-17
• [Presentation] ニワトリ胚口蓋板形成過程でのTbx22の発現調節とその機能 (2008)
  • Author(s): 和田直之, 西松伸一郎, 濃野勉
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] Morphogenesis of the base of the anterior neurocranium by the neural crest cells during chick development. (2008)
  • Author(s): Naoyuki Wada, Shigeru Kuratani, Tstomu Nohno
  • Organizer: 2nd meeting of the European society for evolutionary developmental biology
  • Place of Presentation: Ghent, Belgium
  • Year and Date: 2008-08-01
• [Presentation] Morphogenesis and Origin of the cartilaginous elements of the anterior neurocranium during chick development (2008)
  • Author(s): Naoyuki Wada, Shigeru Kuratani, Tstomu Nohno
  • Organizer: 第41回日本発生生物学会年会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-28
• [Presentation] ニワトリ胚口蓋板形成時におけるTbx22の発現とその調節 (2007)
  • Author(s): 和田 直之, 西松 伸一郎, 濃野 勉
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] 索前軟骨頭蓋は, 異なる神経堤細胞群に由来する二つの構造から構成される.
  • Author(s): 和田直之, 濃野勉, 倉谷滋
  • Organizer: 第115回日本解剖学会総会・全国学術集会
• [Presentation] Expression of Tbx22 in the mouth primordium during Xenopus development.
  • Author(s): 和田直之, 西松伸一郎, 濃野勉
  • Organizer: 第32回日本分子生物学会年会, 年会プログラム
• [Presentation] 3.和田直之, 西松伸一郎, 濃野勉:ニワトリ胚口腔形成過程におけるTbx22の発現とその調節.
  • Author(s): 和田直之, 西松伸一郎, 濃野勉
  • Organizer: 日本動物学会第80回大会
• [Presentation] Morphogenesis and origin of the cartilaginous elements of the anterior neurocranium during chick development.
  • Author(s): 和田直之, 倉谷滋, 濃野勉
  • Organizer: 日本発生生物学会第41回大会
• [Presentation] ニワトリ胚の頭部軟骨骨格の形成と発生系譜.
  • Author(s): 和田直之, 倉谷滋, 濃野勉
  • Organizer: 日本動物学会第79回大会
• [Presentation] ニワトリ胚口蓋板形成過程でのTbx22の発現調節とその機能.
  • Author(s): 和田直之, 西松伸一郎, 濃野勉
  • Organizer: 第31回日本分子生物学会年会