Cytoplasmic sex determination : mitochondrial and maternal factors

• Project/Area Number: 19570219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Hiroshima University
• Principal Investigator: MIURA Ikuo Hiroshima University, 大学院・理学研究科, 准教授 (10173973)
• Co-Investigator(Kenkyū-buntansha): 大谷 浩己 広島大学, 大学院・理学研究科 (20106800)
• Co-Investigator(Renkei-kenkyūsha): OTANI Hiromi 広島大学, 大学院・理学研究科, 助手 (20106800)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: オルガネラ起源 / 性決定 / 細胞質 / ミトコンドリア

Research Abstract

脊椎動物において、卵内に存在する細胞質因子が性決定の重要な役割を担うことを明らかにするため、ツチガエルを用いて、ミトコンドリア由来および、性染色体に由来する細胞質因子の同定を試みた。ミトコンドリアからはD-loop内に存在する8塩基ないし10塩基を単位とするくり返し配列に由来するペプチド因子をみつけ、母性因子としては主に3種類のガレクチン分子が卵内に特異的に存在することを見つけた。機能解析を行うため、DNAコンストラクトを用いたトランスジェネシスに取り組み、ツチガエルにおける核酸マイクロインジェクション法を確立した。

Research Products

• [Journal Article] 2つの性決定機構をもつツチガエル (2009)
  • Author(s): 尾形光昭、三浦郁夫
  • Journal Title: 生物の科学遺伝 NTS.1 Pages: 32-37
• [Journal Article] 2つの性決定機構をもつツチガエル (2009)
  • Author(s): 三浦郁夫
  • Journal Title: 生物科学の遺伝 1 Pages: 32-37
• [Journal Article] The ZZ/ZW sex-determining mechanism originated twice and independently during evolution of the frog (2008)
  • Author(s): Ogata M, Hasegawa Y, Ohtani H, Mineyama M, Miura I
  • Journal Title: Rana rugosa. Heredity 100(1) Pages: 92-99
  • Peer Reviewed
• [Journal Article] Comparative chromosome mapping of sex-linked genes and identification of sex chromosomal rearrangements in the Japanese wrinkled frog(Rana rugosa, Ranidae)with ZW and XY sex chromosome systems (2008)
  • Author(s): Uno Y, Nishida C, Oshima Y, Yokoyama S, Miura I, Matsuda Y, Nakamura M
  • Journal Title: Chromosome Res 16 Pages: 637-647
  • Peer Reviewed
• [Journal Article] Disentangling genetic vs. environmental causes of sex determination in the common frog (2008)
  • Author(s): Matsuba C, Miura I, and Merila J
  • Journal Title: Rana temporaria, BMC Genet 9(1) Pages: 1-5
  • Peer Reviewed
• [Journal Article] 生物進化の矛盾を楽しむ (2008)
  • Author(s): 三浦郁夫
  • Journal Title: 生物工学会誌 86(1) Pages: 35-35
  • NAID: 110006572468
• [Journal Article] 両生類の性決定 : XY型とZW型のちがい (2008)
  • Author(s): 三浦郁夫
  • Journal Title: アニテックス 5月号19(3) Pages: 27-33
• [Journal Article] Comparative chromosome mapping of sex-linked genes and identification of sex chromosomal rearrangements in the Japanese wrinkled frog (Rana rugosa, Ranidae) with ZW and XY sex chromosome systems (2008)
  • Author(s): Uno Y.
  • Journal Title: Chromosorme Research 16 Pages: 637-647
  • Peer Reviewed
• [Journal Article] Disentangling genetic vs. environmental causes of sexdetermination in the common frog, Rana temporaria. (2008)
  • Author(s): Matsuba, C.
  • Journal Title: BMC Genetics 9 Pages: 1-5
  • Peer Reviewed
• [Journal Article] The ZZ/ZW sex-determining mechanism originated twice and independently during evolution of the frog, Rana rugosa. (2008)
  • Author(s): Ogata, M
  • Journal Title: Heredity 100 Pages: 92-99
  • Peer Reviewed
• [Journal Article] An evolutionary witness : the frog Rana rugosa underwent change of heterogametic sex from XY male to ZW female (2007)
  • Author(s): Miura, I
  • Journal Title: Sexual development 1(6) Pages: 323-331
  • Peer Reviewed
• [Journal Article] An evolutionary witness: the frog Rana rugosa, underwent change of heterogametic sex from XY male to ZW female. (2007)
  • Author(s): Miura, I,
  • Journal Title: Sexual development 1 Pages: 323-333
  • Peer Reviewed
• [Journal Article] 両生類の性決定:XY型とZW型のちがい (2007)
  • Author(s): 三浦 郁夫
  • Journal Title: アニテックス 19(3) Pages: 27-33
• [Presentation] Molecular characterization of ZW sex chromosomes and the prototype chromosomes in the frog Rana rugosa (2008)
  • Author(s): Miura I.
  • Organizer: The 3^<rd> Asian chromosome colloquium
  • Place of Presentation: 大阪
  • Year and Date: 2008-12-01
• [Presentation] Molecular cytogenetic study on the origin and evolution of sex chromosomes in different genetic forms of the Japanese wrinkled frog (Rana rugosa, Ranidae). (2008)
  • Author(s): Uno Y.
  • Organizer: The 3^<rd> Asian chromosome colloquium
  • Place of Presentation: 大阪
  • Year and Date: 2008-12-01
• [Presentation] ツチガエルにおけるZ染色体とY染色体の起源と進化 (2008)
  • Author(s): 尾形光昭
  • Organizer: 日本爬虫両生類学会
  • Place of Presentation: 宇都宮(栃木)
  • Year and Date: 2008-10-26
• [Presentation] 広島県に残されたダルマガエル3集団の遺伝的特徴 (2008)
  • Author(s): 小泉雄紀
  • Organizer: 日本爬虫両生類学会
  • Place of Presentation: 宇都宮(栃木)
  • Year and Date: 2008-10-26
• [Presentation] 尻尾のあるトノサマガエル (2008)
  • Author(s): 三浦郁夫
  • Organizer: 日本爬虫両生類学会
  • Place of Presentation: 宇都宮(栃木)
  • Year and Date: 2008-10-26
• [Presentation] An exceptional frog proves the rule of heterogametic sex shift. (2008)
  • Author(s): Ogata M.
  • Organizer: International symposium of gonad and brain sex differentiation
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-16
• [Presentation] Molecular characterization of ZW sex chromosomes and the prototype chromosomes in the frog Rana rugosa (2008)
  • Author(s): Miura, I., Tariq, E., Ohtani, H. and Graves JAM
  • Organizer: The 3^<rd>Asian chromosome colloquim
  • Place of Presentation: Osaka, Japan
• [Presentation] ツチガエルにおけるZ染色体とY染色体の起源と進化 (2008)
  • Author(s): 尾形光昭、三浦郁夫
  • Organizer: 日本爬虫両生類学会第47回年会
  • Place of Presentation: 宇都宮(栃木)
• [Presentation] 広島県に残されたダルマガエル3集団の遺伝的特徴 (2008)
  • Author(s): 小泉雄紀、内藤順一、秋山繁治、藤谷武史、三浦郁夫
  • Organizer: 日本爬虫両生類学会第47回年会
  • Place of Presentation: 宇都宮(栃木)
• [Presentation] 尻尾のあるトノサマガエル (2008)
  • Author(s): 小泉雄紀、藤谷武史、大谷浩己、矢尾板芳郎、三浦郁夫
  • Organizer: 日本爬虫両生類学会第47回年会
  • Place of Presentation: 宇都宮(栃木)
• [Presentation] An exceptional frog proves the rule of heterogametic sex shift (2008)
  • Author(s): Ogata, M., Ohtani, H., Miura, I.
  • Organizer: International symposium of gonad and brain sex differentiation
  • Place of Presentation: Fukuoka, Japan
• [Presentation] 性決定候補遺伝子の機能解析に向けたトランスジェニック・ツチガエルの作成 (2007)
  • Author(s): 三浦 郁夫
  • Organizer: 染色体学会
  • Place of Presentation: 三浦郡葉山町(神奈川)
  • Year and Date: 2007-11-26
• [Presentation] ツチガエルの近畿地方と中国地方の境界領域における遺伝的調査 (2007)
  • Author(s): 尾形 光昭
  • Organizer: 日本爬虫両生類学会
  • Place of Presentation: 沖縄県中頭郡西原町
  • Year and Date: 2007-11-17
• [Presentation] 性決定候補遺伝子の機能解析に向けたトランスジェニッック・ツチガエルの作成 (2007)
  • Author(s): 三浦郁夫、大谷浩己、池野正史、長谷川嘉則
  • Organizer: 染色体学会第58回年会
  • Place of Presentation: 三浦郡葉山町(神奈川)
• [Presentation] ツチガエルの近畿地方と中国地方の境界領域における遺伝的調査 (2007)
  • Author(s): 尾形光昭、大谷浩己、長谷川嘉則、三浦郁夫
  • Organizer: 日本爬虫両生類学会第46回年会
  • Place of Presentation: 沖縄県中頭郡西原町
• [Presentation] Molecular cytogenetic study on the origin and evolution of sex chromosomes in different genetic forms of the Japanese wrinkled frog(Rana rugosa, Ranidae)
  • Author(s): Uno, I., Nishida, C., Oshima, Y., Yokoyama, S., Miura, I., Nakamura, M. and Matsuda, Y.
  • Organizer: The 3^<rd> Asian chromosome colloquim
  • Place of Presentation: Osaka, Japan
• [Book] The W chromosome evolution and sex-linked gene expression in the Japanese frog Rana rugosa(Sex Chromosomes : Genetics, Abnormalities, and Disorders) (2009)
  • Author(s): Miura I, Ezaz T, Ohtani H, Uno Y, Nishida C, Matsuda Y, and Graves JAM
  • Publisher: Nova Science Publishers, Inc., In press
• [Book] Sex Chromosomes : Genetics, Abnormalities, and Disorders (2009)
  • Author(s): Miura I.
  • Publisher: Nova Science Publishers Inc.(印刷中)