Functional analysis of newly acquired genes that directed the evolution of chordates.

• Project/Area Number: 19570220
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Kochi University
• Principal Investigator: FUJIWARA Shigeki Kochi University, 教育研究部自然科学系, 教授 (40229068)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: レチノイン酸 / 発生・分化 / 脊索動物 / 進化 / 遺伝子 / Hox / Nodal / 遺伝子発現調節 / レチノイン酸受容体 / レチノイン酸合成酵素

Research Abstract

I analyzed the function of genes that were involved in the establishment of the chordate-specific body plan. Retinoic acid has been thought to function only in vertebrate embryos. In the present study, I demonstrated that retinoic acid contributed to organogenesis in the ascidian embryo, through the activation of the Hox1 gene. I also demonstrated that the growth factor, Nodal, was required for morphogenesis of the dorsal neural tube, which was a chordate-specific feature. The transcription factor, Cdx, was revealed to mediatethe Nodal signal and regulates neural tube formation.

Research Products

• [Journal Article] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2010)
  • Author(s): Kaneko, N., Katsuyama, Y., Kawamura, K., Fujiwara, S
  • Journal Title: Dev. Growth Difer vol.52 Pages: 457-468
  • NAID: 10027565188
  • Peer Reviewed
• [Journal Article] Identification of genes downstream of Nodal in the Ciona intestinalis embryo (2010)
  • Author(s): Mita, K., Koyanagi, R., Azumi, K., Sabau, S.V., Fujiwara, S
  • Journal Title: Zool. Sci. vol 27 Pages: 69-75
  • NAID: 40017358555
  • Peer Reviewed
• [Journal Article] Identification of genes downstream of Nodal in the Ciona intestinalis embryo (2010)
  • Author(s): Mita, K.
  • Journal Title: Zoological Science 27 Pages: 69-75
  • Peer Reviewed
• [Journal Article] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2010)
  • Author(s): Kaneko, N.
  • Journal Title: Development, Growth & Differentiation 52(印刷中)
  • Peer Reviewed
• [Journal Article] Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo (2009)
  • Author(s): Kanda, M., Wada, H., Fujiwara, S
  • Journal Title: Dev. Biol vol.35 Pages: 454-463
  • NAID: 120001671558
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the retinoic acid receptor in the dorsal midline epidermis in the Ciona intestinalis embryo (2009)
  • Author(s): Koyano, R., Ishida, S., Fujiwara, S
  • Journal Title: Dev. Growth Dife vol.51 Pages: 777-786
  • NAID: 10027562418
  • Peer Reviewed
• [Journal Article] Mechanism of DNA replication-dependent transcriptional activation of the acetylcholinesterase gene in the Ciona intestinalis embryo (2009)
  • Author(s): Kataoka, Y., Mishina, R., Fujiwara, S
  • Journal Title: Dev. Growth Differ vol.51 Pages: 841-850
  • NAID: 10027562643
  • Peer Reviewed
• [Journal Article] Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo (2009)
  • Author(s): Kanda, M.
  • Journal Title: Developmental Biology 335 Pages: 454-463
  • Peer Reviewed
• [Journal Article] Mechanism of DNA replication-dependent transcriptional activation of the acetylcholinesterase gene in the Ciona intestinalis embryo (2009)
  • Author(s): Kataoka, Y.
  • Journal Title: Development, Growth & Differentiation 51 Pages: 841-850
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the retinoic acid receptor in the dorsal midline epidermis in the Ciona intestinalis embryo (2009)
  • Author(s): Koyano, R.
  • Journal Title: Development, Growth & Differentiation 51 Pages: 777-786
  • Peer Reviewed
• [Journal Article] RNA interference by expresing short hairpin RNA in the Ciona intestinalis embryo (2008)
  • Author(s): Nishiyama, A., Fujiwara, S
  • Journal Title: Dev. Growth Differ vol.50 Pages: 521-529
  • Peer Reviewed
• [Journal Article] Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates (2008)
  • Author(s): Kawamura, K., Sugino, Y., Sunanaga, T., Fujiwara, S
  • Journal Title: Dev. Growth Differ vol.50 Pages: 1-11
  • NAID: 10020274014
  • Peer Reviewed
• [Journal Article] RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo. (2008)
  • Author(s): Nishiyama, A.
  • Journal Title: Dev. Growth Differ. 50 Pages: 521-529
  • Peer Reviewed
• [Journal Article] Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates. (2008)
  • Author(s): Kawamura, K.
  • Journal Title: Dev. Growth Differ. 50 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Multipotent epithelial cells in the process of regeneration and asexual reproduction in colonial tunicates (2008)
  • Author(s): Kawamura, K.
  • Journal Title: Develoment, Growth and Differentiation 50 Pages: 1-11
  • Peer Reviewed
• [Journal Article] RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo (2008)
  • Author(s): Nishiyama, A.
  • Journal Title: Develoment, Growth and Differentiation 50(印刷中)
  • Peer Reviewed
• [Journal Article] Nodal regulates neural tube formation in the Ciona intestinalis embryo (2007)
  • Author(s): Mita, K., Fujiwara, S
  • Journal Title: Dev. Genes Evol vol.217 Pages: 593-601
  • NAID: 120001073510
  • Peer Reviewed
• [Journal Article] Gene expresion profile during the life cycle of the urochordate Ciona intestinalis (2007)
  • Author(s): Azumi, K., Sabau, S.V., Fujie, M., Usami, T., Koyanagi, R., Kawashima, T., Fujiwara, S., Ogasawara, M., Satake, M., Nonaka, M., Wang, H-G., Satou, Y., Satoh, N
  • Journal Title: Dev. Biol vol.308 Pages: 572-582
  • Peer Reviewed
• [Journal Article] Gene expression profile during the life cycle of the urochordate Ciona intestinalis (2007)
  • Author(s): Azumi, K.
  • Journal Title: Developmental Biology 308 Pages: 572-582
  • Peer Reviewed
• [Journal Article] Nodal regulates neural tube formation in the Ciona intestinalis embryo (2007)
  • Author(s): Mita, K.
  • Journal Title: Developmelnt, Genes and Evolution 217 Pages: 593-601
  • Peer Reviewed
• [Presentation] Transcriptional regulation of Hox1 by retinoic acid in the Ciona intestinalis embryo (2009)
  • Author(s): Ikeda, T., Fujiwara, S., Kanda, M
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Transcriptional regulation of Hox1 by retinoic acid in the Ciona intestinalis embryo (2009)
  • Author(s): Ikeda, T.
  • Organizer: 日本分子生物学会・第32回年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-11
• [Presentation] カタユウレイボヤ胚におけるアセチルコリンエステラーゼ遺伝子のDNA複製回数依存的な転写調節の仕組み (2009)
  • Author(s): 三科亮
  • Organizer: 日本分子生物学会・第32回年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-11
• [Presentation] カタユウレイボヤ胚の背側正中線表皮におけるチノン酸受容体遺伝子の転写調節 (2009)
  • Author(s): 石田聡美・小谷野竜介・藤原滋樹
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-17
• [Presentation] カタユウレイボヤ胚の背側正中線表皮におけるレチノイン酸受容体遺伝子の転写調節 (2009)
  • Author(s): 石田聡美
  • Organizer: 日本動物学会・第80回大会
  • Place of Presentation: グランシップ(静岡県)
  • Year and Date: 2009-09-17
• [Presentation] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2009)
  • Author(s): Kaneko, N., Katsuyama, Y., Kawamura, K., Fujiwara, S
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: 沖縄
  • Year and Date: 2009-06-24
• [Presentation] Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo (2009)
  • Author(s): Kanda, M., Wada, H., Fujiwara, S
  • Organizer: The 5th International Tunicate Meting
  • Place of Presentation: 沖縄
  • Year and Date: 2009-06-24
• [Presentation] Large scale analysis of Nodal-regulated genes in the Ciona intestinalis embryo (2009)
  • Author(s): Mita, K., Koyanagi, R., Sabau, S., Azumi, K., Fujiwara, S
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: 沖縄
  • Year and Date: 2009-06-24
• [Presentation] Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis (2009)
  • Author(s): Kaneko, N.
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター
  • Year and Date: 2009-06-24
• [Presentation] Transposon-mediated enhancer trap revealed the functions of Hox1 during and after metamorphosis (2009)
  • Author(s): Sasakura, Y., Kanda, M., Horie, T., Satoh, N., Fujiwara, S
  • Organizer: The 5th International Tunicate Meting
  • Place of Presentation: 沖縄
  • Year and Date: 2009-06-22
• [Presentation] Transposon-mediated enhancer trap revealed the functions of Hox1 during and after metamorphosis (2009)
  • Author(s): Sasakura, Y.
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター
  • Year and Date: 2009-06-22
• [Presentation] レチノイン酸によるカタユウレイボヤHox1遺伝子の転写調節 (2009)
  • Author(s): 神田美幸
  • Organizer: 日本動物学会中四国支部会・第61回大会
  • Place of Presentation: 高知大学(高知県)
  • Year and Date: 2009-05-16
• [Presentation] Epidermal expression of Hox1 is directly activated by retinoic acid in the Ciona intestinalis embryo (2009)
  • Author(s): 神田美幸
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター
• [Presentation] Large scale analysis of Nodal-regulated genes in the Ciona intestinalis embryo (2009)
  • Author(s): Mita, K.
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター
• [Presentation] Role of RACK1 homologue in zooidal growth and reproduction of budding tunicates (2009)
  • Author(s): Tatzuke, Y.
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: 沖縄産業支援センター
• [Presentation] Nodal signaling regulates neural tube formation in embryos of the ascidian Ciona intestinalis (2008)
  • Author(s): Mita, K.
  • Organizer: Gradient & Signalling : from chemotaxis to developments
  • Place of Presentation: 沖縄先端大
  • Year and Date: 2008-11-20
• [Presentation] ミサキマメイタボヤの再生におけるレチノイン酸の機能 (2008)
  • Author(s): 金子永基・川村和夫・藤原滋樹
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-07
• [Presentation] ミサキマメイタボヤの再生におけるレチノイン酸の機能 (2008)
  • Author(s): 金子永基
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-07
• [Presentation] ミサキマメイタボヤにおけるmycの発現解析および機能解析 (2008)
  • Author(s): 礒崎崇臣
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-07
• [Presentation] レチノイン酸によるカタユウレイボヤHox1遺伝子の転写調節 (2008)
  • Author(s): 神田美幸・和田洋・藤原滋樹
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-05
• [Presentation] レチノイン酸によるカタユウレイボヤHox1遺伝子の転写調節 (2008)
  • Author(s): 神田美幸
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-05
• [Presentation] Roles of Nodal signaling in the embryo of the ascidian Ciona intestinalis (2008)
  • Author(s): Mita, K., Koyanagi, R., Azumi, K., Fujiwara, S
  • Organizer: The 41st Anual Meeting for the Japanese Society of Developmental Biologists (Jointly sponsored by the International Society of Developmental Biologsits
  • Place of Presentation: 徳島
  • Year and Date: 2008-05-28
• [Presentation] Roles of Nodal signaling in the embryo of the ascidian Ciona intestinalis (2008)
  • Author(s): Mita, K.
  • Organizer: 日本発生生物学会(国際発生生物学会共催)
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-28
• [Presentation] カタユウレイボヤの神経管形成におけるNodalシグナル機能解析 (2007)
  • Author(s): 三田薫・藤原滋樹
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] カタユウレイボヤ胚におけるNodalシグナル標的遺伝子のマイクロアレイ解析 (2007)
  • Author(s): 三田 薫
  • Organizer: 日本分子生物学会第30回年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-11
• [Presentation] ホヤの無性生殖におけるホメオボックス遺伝子のcDNA単離と発現解析 (2007)
  • Author(s): 柏原 央人
  • Organizer: 日本分子生物学会第30回年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-11
• [Presentation] ホヤ胚におけるDNA複製回数依存的な転写調節の仕組みを探る試み (2007)
  • Author(s): 藤原 滋樹
  • Organizer: 第2回ホヤ研究集会
  • Place of Presentation: 下田東急ホテル
  • Year and Date: 2007-05-08
• [Presentation] カタユウレイボヤの神経管形成におけるNodalシグナルの機能解析 (2007)
  • Author(s): 三佃 薫
  • Organizer: 第2回ホヤ研究集会
  • Place of Presentation: 下田東急ホテル
  • Year and Date: 2007-05-07