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Molecular evolution of hatching enzyme of ancient fishes

Research Project

Project/Area Number 19570221
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Evolutionary biology
Research InstitutionSophia University

Principal Investigator

安増 茂樹  Sophia University, 理工学部, 教授 (00222357)

Co-Investigator(Kenkyū-buntansha) 井内 一郎  上智大学, 理工学部, 教授 (10011694)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords分子進化 / 孵化酵素 / 卵膜分解機構 / 機能進化 / チョウザメ / タンパク質機能進化 / プロテアーゼ / 遺伝子進化 / 古代魚 / 硬骨魚類
Research Abstract

申請研究は、表題にある古代魚孵化腺細胞の発生進化学的研究に加え、卵膜分解機構の進化研究の2題を含んでいる。前者においはて、チョウザメより孵化液を調整して、孵化酵素の精製途中である。後者の卵膜分解機構の進化研究では、初期の硬骨魚類孵化酵素の卵膜分解系は、膨潤化酵素の単一酵素であったものが、進化過程で、膨潤卵膜分解酵素が出現して2つの酵素の効率良い卵膜分解系に進化したことが明らかとなった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (21 results)

All 2009 2008 2007

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (10 results)

  • [Journal Article] Different hatching strategies in embryos of two species,pacific herring Clupea pallasii and Japanese anchovy Engraulis japonicus, that belong to the same order Clupeiformes, and their environmental adaptation2009

    • Author(s)
      Kawaguchi M, Fujita H, Yoshizaki N, Hiroi J, Okouchi H, Nagakura Y,Noda T, Watanabe S, Katayama S, Iwamuro S,Nishida M, Iuchi I, Yasumasu S
    • Journal Title

      J.Exp. Zoology B 312

      Pages: 95-107

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Different hatching strategies in embryos of two species, pacific herring Clupea pallasii and Japanese anchovy Engraulis japonicus, that belong to the same order Clupeiformes, and their environmental adaptation.2009

    • Author(s)
      Kawaguchi M, Fujita H, Yoshizaki N, Hiroi J, Okouchi H, Nagakura Y, Noda T, Watanabe S, Katayama S, Iwamuro S, Nishida M, Iuchi I, Yasumasu S
    • Journal Title

      J Exp Zoolog B 312

      Pages: 95-107

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of zebrafish hatching enzyme - an evolutionary aspect of the mechanism of egg envelope digestion2008

    • Author(s)
      Sano K, Inohaya K, Kawaguchi M,YoshizakiN, Iuchi I, Yasumasu S
    • Journal Title

      FEBS J 275

      Pages: 5934-5946

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegeliienvironmental adaptation of the hatching enzyme and evolutionary aspects of formationof the pseudogene.2008

    • Author(s)
      Kawaguchi M, Nakagawa M, Noda T, Yoshizaki N, Hiroi J, Nishida M, Iuchi I, Yasumasu S.
    • Journal Title

      FEBS J 275

      Pages: 2884-2898

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of zebrafish hatching enzymean evolutionary aspect of the mechanism of egg envelope digestion.2008

    • Author(s)
      Sano K, Inohaya K, Kawaguchi M, Yoshizaki N, Iuchi I, Yasumasu S
    • Journal Title

      FEBS J. 275

      Pages: 5934-5946

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegelii-environmental adaptation of the hatching enzyme and evolutionary aspects of formationof the pseudogene.2008

    • Author(s)
      Kawaguchi M, Nakagawa M, Noda T, Yoshizaki N, Hiroi J, Nishida M, Iuchi I, Yasumasu S.
    • Journal Title

      FEBS J. 275

      Pages: 2884-2898

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of the exon-intron structures of fish, amphibian, bird and mammalian hatching enzyme genes, with special reference to the intron loss evolution of hatching enzyme genes in Teleostei2007

    • Author(s)
      Kawaguchi M, Yasumasu S, Hiroi J, Naruse K, Suzuki T, Iuchi I
    • Journal Title

      Gene 392

      Pages: 77-88

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of the exon-intron structures of fish, amphibian, bird and mammalian hatching enzyme genes, with special reference to the intron loss evolution of hatching enzyme genes in Teleostei.2007

    • Author(s)
      Kawaguchi M, Yasumasu S, Hiroi J, Naruse K, Suzuki T, Iuchi I.
    • Journal Title

      Gene. 392

      Pages: 77-88

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Globin gene enhancer activity of a DNase-I hypersensitive site-40homolog in medaka,Oryzias latipes2007

    • Author(s)
      Maruyama K., Ishikawa Y., Yasumasu S., Iuchi I.
    • Journal Title

      Zoolog Sci 24

      Pages: 997-1004

    • NAID

      40016396313

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of the exon-intron structures of fish,amphibian,bird and mammalian hatching enzyme genes,with special reference to the intron loss evolution of hatching enzyme genes in Teleostei2007

    • Author(s)
      Kawaguchi M., Yasumasu S., Hiroi J., Naruse K., Suzuki T., Iuchi I.
    • Journal Title

      Gene.2007 392

      Pages: 77-88

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and expression of xP1-L,a new marker gene for larval surface mucous cells of tadpole stomach in Xenopus laevis2007

    • Author(s)
      Ikuzawa M., Yasumasu S., Kobayashi K., Iuchi I.
    • Journal Title

      Gene Exp Patterns 8

      Pages: 12-8

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 硬骨魚類孵化酵素の卵膜分解機構における機能進化2008

    • Author(s)
      佐野香織, 川口眞理, 井内一郎, 安増茂樹
    • Organizer
      日本進化学会第10回大会
    • Place of Presentation
      東京大学
    • Year and Date
      2008-08-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] 孵化酵素LCEの種特異的卵膜分解2008

    • Author(s)
      川口眞理, 清水昭男, 廣井準也, 井内一郎, 安増茂樹, 西田睦
    • Organizer
      日本進化学会第10回大会
    • Place of Presentation
      東京大学
    • Year and Date
      2008-08-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] 孵化酵素LCE の種特異的卵膜分解2008

    • Author(s)
      川口眞理、清水昭男、廣井準也、井内一郎、安増茂樹、西田睦
    • Organizer
      日本進化学会第9回大会
    • Place of Presentation
      東京大学
    • Related Report
      2008 Final Research Report
  • [Presentation] ウナギ孵化酵素の卵膜分解特異性から考察した卵膜分解機構の進化2008

    • Author(s)
      佐野香織川口眞理井内一郎安増茂樹
    • Organizer
      第79 回日本動物学会
    • Place of Presentation
      福岡大
    • Related Report
      2008 Final Research Report
  • [Presentation] 硬骨魚類孵化酵素の卵膜分解機構における機能進化2008

    • Author(s)
      佐野香織、川口眞理、井内一郎、安増茂樹
    • Organizer
      日本進化学会第9回大会
    • Place of Presentation
      東京大
    • Related Report
      2008 Final Research Report
  • [Presentation] ゼブラフィッシュの孵化酵素とその卵膜分解機構2007

    • Author(s)
      佐野香織安増茂樹猪早敬二吉崎範夫川口眞理井内一郎
    • Organizer
      第13 回小型魚類研究会
    • Place of Presentation
      東京大
    • Related Report
      2008 Final Research Report
  • [Presentation] ウナギ孵化酵素の精製とその卵膜分解機構2007

    • Author(s)
      佐野香織安増茂樹吉川昌之川口眞理井内一郎
    • Organizer
      第78 回日本動物学会
    • Place of Presentation
      弘前大
    • Related Report
      2008 Final Research Report
  • [Presentation] 卵胎生魚クロソイの孵化酵素LCE 遺伝子における偽遺伝子形成機過程2007

    • Author(s)
      川口眞理、安増茂樹、廣井準也、中川雅弘、野田勉、井内一郎
    • Organizer
      日本進化学会第9回大会
    • Place of Presentation
      京都大学
    • Related Report
      2008 Final Research Report
  • [Presentation] 単一酵素系としてのゼブラフィッシュ胚の孵化酵素2007

    • Author(s)
      佐野香織安増茂樹猪早敬二吉崎範夫川口眞理井内一郎
    • Organizer
      第59 回日本動物学会・関東支部会
    • Place of Presentation
      首都大学
    • Related Report
      2008 Final Research Report
  • [Presentation] ウナギ艀化酵素の精製とその卵膜分解機構2007

    • Author(s)
      佐野、安増、吉川、川口、井内
    • Organizer
      日本動物学会
    • Place of Presentation
      弘前大学
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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