Structure and function of enzymes involved with the catabolisation of GABA in Bacillus subtilis.

• Project/Area Number: 19579003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Toho University
• Principal Investigator: GOTO Masaru Toho University, 理学部, 講師 (80379289)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,870,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: X線結晶解析 / 酵素反応 / 立体構造

Research Abstract

The recombinant succinate semialdehyde dehydrogenase with His-tag was purified in one chromatographic step by using Ni-NTA resin. The structures of GABA aminotransferase have determined at acidic and neutral pH. Crystals of GabR protein was obtained by the hanging-drop vapor-diffusion method. The X-ray diffraction data set for the native crystal was collected to 2.2Å resolution. The phase of native GabR was solved by the multiple isomorphous replacement method, using Hg derivative data sets. Now the structure of native GabR is under construction.

Research Products

• [Journal Article] Reaction mechanism and molecular basis for selenium/sulfur discrimination of selenocysteine lyase (2010)
  • Author(s): Omi R.
  • Journal Title: J Biol Chem. 285 Pages: 12133-9
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• [Journal Article] Serine racemase with catalytically active lysinoalanyl residue. (2009)
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• [Journal Article] Crystal Structure of Monofunctional Histidinol Phosphate Phosphatase from Thermus thermophilus HB8. (2007)
  • Author(s): Omi R, Goto M, Miyahara I, Manzoku M, Ebihara A, Hirotsu K.
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• [Presentation] アカガイ由来アスパラギン酸ラセマーゼのX線結晶構造解析 (2009)
  • Author(s): 後藤勝
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] 酢酸菌由来膜結合型アルコール脱水素酵素の結晶構造解析 (2009)
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  • Organizer: 第26回PFシンポジウム
  • Place of Presentation: 筑波
• [Presentation] Role of pyridoxal 5'-phosphate and・-amino butyrate of GabR, a transcriptional regulator of Bacillus subtilis (2008)
  • Author(s): Tohru Yoshimura, Keita Okuda, Hisashi Hemmi, Masaru Goto, Harumi Fukada
  • Organizer: Second International Interdisciplinary Conference on Vitamins, Coenzymes and Biofactors
  • Place of Presentation: GEORGIA, USA
  • Year and Date: 2008-10-02
• [Presentation] ピリドキサルリン酸に依存する細菌の転写制御因子, GabRの構造と機能 (2008)
  • Author(s): 吉村徹, 奥田啓太, 邉見久, 深田はるみ, 後藤勝
  • Organizer: 日本ビタミン学会第60回大会
  • Place of Presentation: 仙台
  • Year and Date: 2008-06-14
• [Presentation] アカガイ由来アスパラギン酸ラセマーゼの立体構造解析 (2008)
  • Author(s): 後藤 勝
  • Organizer: 日本ビタミン学会第60回大会
  • Place of Presentation: 仙台
• [Presentation] Structural study of putative aminotransferase from Thermus thermophllus HB8 (2008)
  • Author(s): Ikuko Miyahara
  • Organizer: XXI Congress of the International Union of Crystal lography
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• [Presentation] Structure of membrane-bound quinohemoprotein alcohol dehydrogenase (2008)
  • Author(s): Masaru Goto
  • Organizer: XXI Congress of the International Union of Crystallography
  • Place of Presentation: Osaka
• [Presentation] Role of pyridoxal 5'-phosphate and γ-aminobutyrate of GabR, a transcriptional regulator of Bacillus subtilis (2008)
  • Author(s): Tohru Yoshimura
  • Organizer: Second International Interdisciplinary Conference onVitamins, Coenzymes and Biofactors
  • Place of Presentation: GEORGIA、USA
• [Presentation] X-ray Structure of membrane-bound quinohemoprotein alchol dehydrogenase (2008)
  • Author(s): Ikuko Miyahara
  • Organizer: Second International Interdisciplinary Conference onVitamins, Coenzymes and Biofactors
  • Place of Presentation: GEORGIA、USA
• [Presentation] Analysis of structure and function of membrane-bound alcohol dehydrogenase from acetic acid bacteria (2008)
  • Author(s): Masaru Goto
  • Organizer: The 2nd International Conference on Acetic Acid Bacteria
  • Place of Presentation: Nagoya
• [Presentation] ピリドキサルリン酸に依存する細菌の転写制御因子、GabRの構造と機能 (2007)
  • Author(s): 奥田啓太, 深田はるみ, 後藤勝, 邉見久, 吉村徹
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Crystal Structures of Nonoxidative Zn-Dependent 2,6-Dihydroxybenzoate Decarboxylase. (2007)
  • Author(s): Goto, M., Hayashi, H., Yoshida, M., Oikawa, T., Miyahara, I., Hirotsu, K.
  • Organizer: 2nd International Symposium on Diffraction Structural Biology 2007,
  • Place of Presentation: タワーホール船堀、東京
• [Presentation] 酢酸菌由来表在性膜タンパク質アルコール脱水素酵素の構造解析 (2007)
  • Author(s): 後藤 勝、宮原郁子、広津 建、松下一信
  • Organizer: 2007年度 酵素・補酵素研究会
  • Place of Presentation: 倉敷アイビースクエア、岡山
• [Presentation] 酢酸菌アルコール脱水素酵素の立体購造 (2007)
  • Author(s): 後藤勝、宮原郁子、広津建、小林由宜、中柄朋子、外山博英、足立収生、松下一信
  • Organizer: 日本生体エネルギー研究会 第33 回討論会
  • Place of Presentation: 山口大学大学会館、山口
• [Presentation] γ-レゾルシン酸脱炭酸酵素の結晶構造解析 (2007)
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  • Organizer: 日本結晶学会 2007年度 年会
  • Place of Presentation: 東京工業大学 大岡山キャンパス、東京
• [Presentation] ピリドキサルリン酸に依存する細菌の転写制御因子、GabRの構造と機能 (2007)
  • Author(s): 奥田啓太、深田はるみ、後藤 勝、邉見 久、吉村 徹
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜、神奈川