| Project/Area Number |
19580004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Tottori University |
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Principal Investigator |
MATSUMOTO Teruyuki Tottori University, 農学部, 教授 (60132825)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Shigeyuki 財団法人日本きのこセンター, 菌蕈研究所, 上席主任研究員 (00072794)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 食用担子菌類 / 胞子欠損性変異 / 連鎖解析 / AFLPマーカー / 分子育種 / ウスヒラタケ |
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| Research Abstract |
In this study, we constructed the genetic linkage map of the sporeless mutant of Pleurotus pulmonarius based on the DNA markers, and determined the locus of sporeless mutation. Moreover, we obtained the DNA markers which are very tightly linked to this locus. These results enable to carry out the efficient breeding of a sporeless mushrooms. In addition, we cloned the region related to this mutation, and based on the information of linkage map we determined the whole nucleotide sequences of this region. By the comparison of the sequences from mutant and wild type, we found the differences in several gene structures. In future, we will identify the gene that causes the sporeless mutation of this fungus by using the complementation test and so on.
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