Molecular biological studies on hybrid lethality of interspecific hybrid of genus Nicotiana

• Project/Area Number: 19580006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Kyoto Prefectural University
• Principal Investigator: MINO Masanobu Kyoto Prefectural University, 生命環境科学研究科, 教授 (90326215)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 植物育種・遺伝 / 生殖隔離 / トランスクリプトーム / プロテオーム / 細胞生理学 / 雑種致死 / 培養細胞 / サブトラクトcDNAライブラリ= / 一酸化窒素 / 活性酸素 / サブトラクトcDNAライブラリー / アネキシン / 細胞死抑制 / 欠失突然変異 / cDNAライブラリー / 熱ショックタンパク質 / TOFF-MS

Research Abstract

Plant breeding based on hybridization between far-related species is important for the sustainable agriculture while climate change around the world will be more problematic. But this technique has a major drawback due to difficulties of establishing the hybrids among the parents that are reproductively isolated from each other. This research project aimed to specify the genes involved in reproductive isolation, delineate features of cell death of which induce hybrid lethality. We isolated and characterized some genes which related to hybrid cell death. We also found that hybrid lethality expresses through programmed cell death based on several cellular signaling cues.

Research Products

• [Journal Article] Shuqun Zhang and Masayoshi Inoue, Hybrid lethality in interspecific F1 hybrid Nicotiana gossei x N. tabacum involves MAP-kinases signaling cascade (2007)
  • Author(s): Masanobu Mino, Mami Kubota, Takasuke Nogi, Shuqun Zhang, Masayoshi Inoue
  • Journal Title: Plant Biology 9巻 Pages: 366-273
  • Peer Reviewed
• [Journal Article] Cell death in seedlings of the interspecific hybrid of Nicotiana gossei and N. tabacum; Possible role of knob-like bodies formed on tonoplast in vacuolar-collapse-mediated cell death. (2007)
  • Author(s): Masanobu Mino, Nachi Murata, Shuichi Date,, Masayoshi Inoue
  • Journal Title: Plant Cell Rep 26巻 Pages: 407-419
  • Peer Reviewed
• [Journal Article] Hybrid lethality in interspecific F_1 hybrid Nicotiana gossei x N. tabacum involves MAP-kinases signaling cascade (2007)
  • Author(s): Masanobu Mino, Mami Kubota, Takasuke Nogi, Shuqun Zhang and Masayoshi Inoue
  • Journal Title: Plant Biology 9 Pages: 366-273
  • Peer Reviewed
• [Journal Article] Cell death in seedlings of the interspecific hybrid of Nicotiana gossei and N. tabacum; Possible role of knob-like bodies formed on tonoplast in vacuolar-collapse-mediated cell death (2007)
  • Author(s): Masanobu Mino, Nachi Murata, Shuichi Date, and Masayoshi Inoue
  • Journal Title: Plant Cell Report 26 Pages: 407-419
  • Peer Reviewed
• [Presentation] タバコ種間雑種(Nicotiana gossei Domin×N.tabacum L.)培養細胞の細胞死における一酸化窒素(NO)の役割 (2010)
  • Author(s): 山本拓海、〓村幸子、小川健一、三野真布
  • Organizer: 第51回 日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-18
• [Presentation] タバコ種間雑種(Nicotiana gossei Domin x N. tabacum L. )培養細胞の細胞死における一酸化窒素(NO)の役割 (2010)
  • Author(s): 山本拓海、■村幸子、小川健一、三野真布
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] Hybrid lethality : Proteins and genes involved in the cell death of F1 hybrid between Nicotiana gossei Domin and N.tabacum L. (2009)
  • Author(s): Masanobu Mino, Takumi Yamamoto, Seitaro Aisu, Hanako Okumura, Kenji Sato, Sakihito Kitajima, Yoshikazu Tanaka
  • Organizer: 第9回 国際植物分子生物学会(IPMB)
  • Place of Presentation: セントルテス・アメリカ合衆国
  • Year and Date: 2009-10-26
• [Presentation] タバコ種間雑種(Nicotiana gossei x N. tabacum)の雑種致死を引き起こす培養細胞に特異的に発現するタンパク質と遺伝子 (2009)
  • Author(s): 三井涼子, 山本拓海, 愛洲清太郎, 奥村華子, 浅田由起子, 佐藤健司, 北島佐紀人, 田中良和, 三野真布
  • Organizer: 第50回 日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] タバコ種間雑種(Nicotiana gossei x N. tabacum)の雑種致死を引き起こす培養に特異的に発現するタンパク質と遺伝子 (2009)
  • Author(s): 三井涼子、山本拓也、愛洲清太郎、奥村華子、浅田由起子、佐藤健司、北島佐紀人、田中良和、三野真布
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
• [Presentation] Proteins and genes involved in the cell death of F1 hybrid between Nicotiana gossei Domin x N. tabacum L. (2009)
  • Author(s): Msanobu Mino, Takumi Yamamoto, Seitaro Aisu, Hnako Okumura, Kenji Sato, Sakihito Kitajima, Yoshikazu Tanaka, Hybrid lethality
  • Organizer: 第9回国際植物分子生物学会、セントルイス
  • Place of Presentation: アメリカ合衆国
• [Remarks]
  • URL: http://www2.kpu.ac.jp/life_environ/plant_breeding/index.html
• [Remarks]
  • URL: http://www2.kpu.ac.jp/life_environ/plant_breeding/index.html
• [Remarks]
  • URL: http://www2.kpu.ac.jp/life_environ/plant_breeding/index.html