Functional analyses of genes conferring insecticide resistance and their application in control of Plutella xylostella

• Project/Area Number: 19580055
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Okayama University
• Principal Investigator: SONODA Shoji Okayama University, 資源生物科学研究所, 准教授 (00325127)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: コナガ / 殺虫剤抵抗性 / ピレスロイド / ナトリウムチャネル / チトクロームP450 / 選択的スプライシング / 合成ピレスロイド

Research Abstract

Pyrethroid resistance of the diamondback moth Plutella xylostella is mainly conferred by increased metabolic detoxification of the degradation enzymes and decreased sensitivity of the target site. Analysis of amino acid sequences of the sodium channel showed that three point mutations (L1014F, T929I and M918I) and two polymorphisms (A1101T and P1879S) on the channel are involved in the pyrethroid resistance. The T929I site was encoded by two mutually exclusive alternative exons (18a and 18b) and the splice variant containing 18b was expressed in a tissue specific manner. Analysis of the CYP6BG1 expression and the sodium channel mutations showed that both resistance mechanisms are independently involved in pyrethroid resistance of the diamondback moth in the field.

Research Products

• [Journal Article] Molecular analysis of pyrethroid resistance conferred by target insensitivity and increased metabolic detoxification in Plutella xylostella. (2010)
  • Author(s): Sonoda, S
  • Journal Title: Pest Management Science (available on line)
• [Journal Article] Molecular analysis of pyrethroid resistance conferred by target insensitivity and increased metabolic detoxification in Plutella xylostella (2010)
  • Author(s): Shoji Sonoda
  • Journal Title: Pest Management Science 66 Pages: 572-575
  • Peer Reviewed
• [Journal Article] Alternative splicing of para-sodium channelα-subunit genes from diamondback moth strains with different sensitivities to a pyrethroid. (2009)
  • Author(s): Sonoda, S
  • Journal Title: Journal of Pesticide Science 34 Pages: 173-176
  • NAID: 40016807862
• [Journal Article] Alternative splicing of para-sodium channel α-subunit genes from diamondback moth strains with different sensitivities to a pyrethroid (2009)
  • Author(s): Shoji Sonoda
  • Journal Title: Journal of Pesticide Science 34 Pages: 173-176
  • NAID: 40016807862
  • Peer Reviewed
• [Journal Article] Alternatively spliced sodium channel transcripts expressed in field strains of the diamondback moth. (2008)
  • Author(s): Sonoda, S., Igaki, C., Tsumuki, H
  • Journal Title: Insect Biochemistry and Molecular Biology 38 Pages: 883-890
• [Journal Article] Genomic organization of the para-sodium channelα-subunit genes from the pyrethroid-resistant and-susceptible strains of the diamondback moth. (2008)
  • Author(s): Sonoda, S., Tsukahara, Y. Ashfaq, M., Tsumuki, H
  • Journal Title: Archives of Insect Biochemistry and Physiology 69 Pages: 1-12
• [Journal Article] Gene expression of hsp70 of the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae), in response to heat shock and insecticides. (2008)
  • Author(s): Sonoda, S., Tsumuki, H
  • Journal Title: Applied Entomology and Zoology 43 Pages: 241-247
  • NAID: 110006684226
• [Journal Article] Gene expression of hsp70 of the diamondback moth. Plutella xylostella (Lepidoptera : Yponomeutidae). in response to heat shock and insecticides (2008)
  • Author(s): Sonoda, S., Tsumuki, H.
  • Journal Title: Applied Entomology and Zoology 43 Pages: 241-247
  • NAID: 110006684226
  • Peer Reviewed
• [Journal Article] Genomic organization of the para-sodium channel α-subunit genes from the pyrethroid-resistant and -susceptible strains of the diamondback moth (2008)
  • Author(s): Sonoda. S., Tsukahara, Y., Ashfaq. H.
  • Journal Title: Archives of Insect Biochemistry and Physiology 69 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Alternatively spliced sodium channel transcripts expressed in field strains of the diamondback moth (2008)
  • Author(s): Sonoda, S., Igaki. C., Tsumuki. H.
  • Journal Title: Insect Biochemistry and Molecular Biology 38 Pages: 883-890
  • Peer Reviewed
• [Journal Article] Gene expression of hsp70 of the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae), in response to heat shock and insecticides (2008)
  • Author(s): Sonoda, S. and Tsumuki, H.
  • Journal Title: Applied Entomology and Zoology 43(in press)
  • NAID: 110006684226
  • Peer Reviewed
• [Journal Article] Genomic organization of the para-sodium channel α-subunit genes from the pyrethroid-resistant and-susceptible strains of the diamondback moth (2008)
  • Author(s): Sonoda, S., Tsukahara, Y., Ashfaq, M. a〓
  • Journal Title: Archives of Insect Biochemistry and Physiology 67(in press)
  • Peer Reviewed
• [Journal Article] Expression of two methionine-rich storage protein genes of Plutella xylostella (L. ) in response to development, juvenile hormone-analog and pyrethroid. (2007)
  • Author(s): Ashfaq, M., Sonoda, S., Tsumuki, H
  • Journal Title: Comparative Biochemistry and Physiology, Part B 148 Pages: 84-92
• [Journal Article] Expression of two methionine-rich storage protein genes of Plutella xylostella (L.)in response to development, juvenile hormone-analog and pyrethroid (2007)
  • Author(s): Ashfaq, M., Sonoda, S and Tsumuki, H.
  • Journal Title: Comparative Biochemistry and Physiology, Part B 148 Pages: 84-92
  • Peer Reviewed
• [Presentation] Molecular analysis of pyrethroid resistance conferred by target insensitivity and increased metabolic detoxification in Plutella xylostella. (2009)
  • Author(s): Sonoda, S
  • Organizer: Japan-Korea Joint Seminar-New Trends in Insecticide Resistance Management-
  • Place of Presentation: Seoul National University, Seoul, Korea
  • Year and Date: 2009-11-20
• [Presentation] Molecular analysis of pyrethroid resistance conferred by target insensitivity and increased metabolic detoxification in Plutella xylostella (2009)
  • Author(s): Shoji Sonoda
  • Organizer: Japan-Korea Joint Seminar -New Trends in Insecticide Resistance Management-
  • Place of Presentation: Seoul National University, Seoul. Korea
  • Year and Date: 2009-11-20
• [Presentation] コナガの合成ピレスロイド剤抵抗性とナトリウムチャネルの発現 (2009)
  • Author(s): 園田昌司
  • Organizer: 第53回日本応用動物昆虫学会大会小集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] コナガの合成ピレスロイド剤抵抗性に関与する新たなナトリウムチャネルの変異について (2009)
  • Author(s): 園田昌司・井垣智賀子・積木久明
  • Organizer: 第53回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-03-30
• [Presentation] コナガの合成ピレスロイド剤抵抗性および感受性系統におけるナトリウムチャネル・アルファサブユニット遺伝子の選択的スプライシング (2008)
  • Author(s): 園田昌司・積木久明
  • Organizer: 第52回日本応用動物昆虫学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2008-03-26
• [Presentation] Amino acid substitution and alternative exon usage of the para-sodium channelα-subunit genes from strains of the diamondback moth with different sensitivity to a pyrethroid. (2008)
  • Author(s): Sonoda, S
  • Organizer: 4th Pan Pacific Conference of Pesticide Science. Honolulu
  • Place of Presentation: Hawaii, USA
• [Presentation] Ami no acid substitution and alternative exon usage of the para-sodium channel α-subunit (2008)
  • Author(s): Sonoda, S.
  • Organizer: 4th Pan Pacific Conference of Pesticide Science
  • Place of Presentation: Honolulu, Hawaii. USA