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Establishment of RNAi methodology to Bombyx mori strain P50 and application for lepidopteran insects

Research Project

Project/Area Number 19580061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

OHNISHI Atsushi  The Institute of Physical and Chemical Research, 松本分子昆虫学研究室, 協力研究員 (50342762)

Research Collaborator MATSUMOTO Shogo  独立行政法人理化学研究所, 松本分子昆虫学研究室, 主任研究員 (60134516)
ATSUSAWA Shinji  独立行政法人理化学研究所, 松本分子昆虫学研究室, 先任技師
KURIHARA Masaaki  独立行政法人理化学研究所, 松本分子昆虫学研究室, 先任技師
HASHIMOTO Kana  法政大学, 大学院工学研究科・物質化学専攻, 修士課程
KAJI Misato  埼玉大学, 大学院理工学研究科・生命科学系専攻, 博士前期課程
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords性フェロモン / ボンビコール / カイコガ / RNAi / フェロモン腺 / lipase perilipin / FATP / TG liase
Research Abstract

チョウ目(鱗翅目)昆虫でのRNA 干渉(RNAi)法の確立を目指し、カイコガ純系系統P50 を用いてRNAi 効果の検証を行った。その結果、性フェロモン産生器官であるフェロモン腺で発現する遺伝子に対して、RNAi は効果的に作用することが確認できた。カイコガ性フェロモン(ボンビコール)産生メカニズムに関与する機能分子の機能解析にRNAi を利用した結果、lipase、Perilipin、FATP を含む新規8 遺伝子がボンビコール産生に関与することが示された。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (32 results)

All 2009 2008 2007

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (16 results) Book (4 results)

  • [Journal Article] Functional characterization of the Bombyx mori fatty acid transport protein (BmFATP) within the silkmoth pheromone gland2009

    • Author(s)
      Ohnishi, A., Hashimoto, K., Imai, K.,and Matsumoto, S.
    • Journal Title

      J. Biol. Chem. 284

      Pages: 5128-5136

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms underlying PBAN signaling in the silkmoth2009

    • Author(s)
      Matsumoto, S., J. Joe Hull., & Ohnishi, A.
    • Journal Title

      Bombyx mori. Ann. NY Acad. Sci. 1163

      Pages: 464-468

    • Related Report
      2008 Final Research Report
  • [Journal Article] Functional characterization of the Bombyx mori fatty acid transport protein (BmFATP) within the silkmoth pheromone gland2009

    • Author(s)
      Ohnishi Atsushi
    • Journal Title

      The Journal of Biological Chemistry 284

      Pages: 5128-5136

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms underlying PBAN signaling in the silkmoth, Bombyx mori2009

    • Author(s)
      Matsumoto Shogo
    • Journal Title

      Annals of the New York Academy of Sciences

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and biological activities of analogs of D-glucosyl-L-tyrosine, a humoral factor that stimulates transcription of the acyl-CoA binding protein in the pheromone gland of the Silkmoth2007

    • Author(s)
      Takahashi, S., Hasumi, K., Ohnishi, A., Koshino, H., & Matsumoto, S.
    • Journal Title

      Bombyx mori. Bioorg. Med. Chem. 15

      Pages: 97-103

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of the pheromone producing region that has epoxidation activity in the abdominal tip of the Japanese giant looper2007

    • Author(s)
      Fujii, T., Suzuki, G. M., Kawai, T., Tsuneizumi, K., Ohnishi, A., Kurihara, M., Matsumoto, S., & Ando, T.
    • Journal Title

      Ascotis selenaria cretacea (Lepidoptera: Geometridae). J. Insect Physiol. 53

      Pages: 312-318

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a unique pheromonotropic neuropeptide including double FXPRL motifs from a geometrid species, Ascotis selenaria cretacea, which produces an epoxyalkenyl sex pheromone2007

    • Author(s)
      Kawai, T., Ohnishi, A., Suzuki, G. M., Fujii, T., Matsuoka, K., Kato, I., Matsumoto, S., & Ando, T.
    • Journal Title

      Insect Biochem. Mol. Biol. 37

      Pages: 330-337

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms underlying sex pheromone production in the silkmoth, Bombyx mori: characterization of the molecular components involved in bombykol biosynthesis2007

    • Author(s)
      Matsumoto, S., Hull, J. J., Ohnishi, A., Moto, K., & Fonagy, A.
    • Journal Title

      J. Insect Physiol. 53

      Pages: 752-759

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and biological activities of analogs of D-glucosyl-L-tyrosine, a humoral factor that stimulates transcription of the acyl-CoA binding protein in the pheromone gland of the Silkmoth, Bombyx mori..2007

    • Author(s)
      Takahashi Shunya
    • Journal Title

      Bioorganic & Medicinal Chemistry 15

      Pages: 97-103

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of the pheromone producing region that has epoxidation activity in the abdominal tip of the Japanese giant looper, Ascotis selenaria cretacea(Lepidoptera: Geometridae).2007

    • Author(s)
      Fujii Takeshi
    • Journal Title

      Journal of Insect Physiology 53

      Pages: 312-318

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a unique pheromonotropic neuropeptide including double FXPRL motifs from a geometrid species, Ascotis selenaria cretacea, which produces an epoxyalkenyl sex pheromone.2007

    • Author(s)
      Kawai Takeshi
    • Journal Title

      Insect Biochemistry and Molecular Biology 37

      Pages: 330-337

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms underlying sex pheromone production in the silkmoth,Bombyx mori:characterization of the molecular components involved in bombybykol biosynthesis.2007

    • Author(s)
      Matusmoto shogo
    • Journal Title

      Journal of Insect Physiology 53

      Pages: 752-759

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] カイコガフェロモン腺で発現する脂肪酸輸送タンパク質(FATP)の機能解析2009

    • Author(s)
      大西敦、橋本佳奈、今井清博、松本正吾
    • Organizer
      日本農芸化学会平成21年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Final Research Report
  • [Presentation] カイコガフェロモン腺発現遺伝子が脂肪滴の形成・分解に与える影響2009

    • Author(s)
      加治美里、大西敦、松本正吾
    • Organizer
      日本農芸化学会平成21年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Final Research Report
  • [Presentation] カイコガPBAN 受容体(PBANR)アイソフォームの同定とその遺伝子発現解析2009

    • Author(s)
      李載〓、J.Joe Hull、本賢一、大西敦、永田宏次、長澤寛道、松本正吾
    • Organizer
      日本農芸化学会平成21年度大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Final Research Report
  • [Presentation] カイコガフェロモン腺で発現する脂肪酸輸送タンパク質(FATP)の機能解析2009

    • Author(s)
      大西敦
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      マリンメッセ福岡、福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] カイコガフェロモン腺発現遺伝子が脂肪滴の形成・分解に与える影響2009

    • Author(s)
      加治美里
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      マリンメッセ福岡、福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] カイコガPBAN受容体(PBANR)アイソフォームの同定とその遺伝子発現解析2009

    • Author(s)
      李載〓
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      マリンメッセ福岡、福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] ボンビコール生合成経路に関与するリパーゼの解析2008

    • Author(s)
      大西敦・橋本佳奈・今井清博・松本正吾
    • Organizer
      日本農芸化学会平成20年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] ボンピコール生合成経路に関与するリパーゼの解析2008

    • Author(s)
      大西 敦
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学、名古屋
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Molecular mechanisms underlying PBAN signaling in the silkmoth, Bombyx mori2008

    • Author(s)
      Matsumoto S., Hull J. J., and Ohnishi A.
    • Organizer
      24th European Conference of European Comparative Endocrinologists
    • Place of Presentation
      Genoa, Italy
    • Related Report
      2008 Final Research Report
  • [Presentation] Isolation and characterization of intracellular proteins that are phosphorylated in response to PBAN stimulation.2007

    • Author(s)
      Ohnishi Atsushi
    • Organizer
      4th Asia-Pacific Conference on Chemical Ecology
    • Place of Presentation
      Tsukuba,Japan
    • Year and Date
      2007-09-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] RNAi 法を用いたカイコガフェロモン腺特異的遺伝子の機能解析2007

    • Author(s)
      大西敦・松本正吾
    • Organizer
      バイオアーキテクトシンポジウム2007
    • Place of Presentation
      和光
    • Year and Date
      2007-09-11
    • Related Report
      2008 Final Research Report
  • [Presentation] PBAN 刺激によりリン酸化を受けるタンパク質の単離、同定2007

    • Author(s)
      大西敦・松本正吾
    • Organizer
      日本農芸化学会平成19年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-25
    • Related Report
      2008 Final Research Report
  • [Presentation] カイコガフェロモン腺特異的遺伝子の検索と機能解析2007

    • Author(s)
      橋本佳奈・大西敦・本賢一・今井清博・松本正吾
    • Organizer
      日本農芸化学会平成19年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-25
    • Related Report
      2008 Final Research Report
  • [Presentation] シャクガ科昆虫のフェロモン生合成活性化神経ペプチドに関する研究2007

    • Author(s)
      河合岳志・大西敦・藤井毅・鈴木雅京・松本正吾・安藤哲
    • Organizer
      日本農芸化学会平成19年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-25
    • Related Report
      2008 Final Research Report
  • [Presentation] Isolation and characterization of intracellular proteins that are phosphorylated in response to PBAN stimulation2007

    • Author(s)
      Ohnishi, A., & Matsumoto, S.
    • Organizer
      4th Asia-Pacific Conference on Chemical Ecology
    • Place of Presentation
      Tsukuba
    • Related Report
      2008 Final Research Report
  • [Presentation] Determination of the PBAN receptor (PBANR) in the Japanese giant looper Ascotis selenaria cretacea which produces an epoxyalkenyl sex pheromone2007

    • Author(s)
      Kawai, T., Ohnishi, A., Matsumoto, S., & Ando, T.
    • Organizer
      4th Asia-Pacific Conference on Chemical Ecology
    • Place of Presentation
      Tsukuba
    • Related Report
      2008 Final Research Report
  • [Book] 第三章-昆虫は化学の力で恋をする、入門ケミカルバイオロジー 47-632008

    • Author(s)
      大西敦
    • Publisher
      オーム社
    • Related Report
      2008 Final Research Report
  • [Book] 入門ケミカルバイオロジー2008

    • Author(s)
      大西敦
    • Total Pages
      17
    • Publisher
      オーム社
    • Related Report
      2008 Annual Research Report
  • [Book] 昆虫の配偶行動と性フェロモン産生、蛋白質核酸酵素増刊ケミカルバイオロジー Vol.52 No.13 1679-16842007

    • Author(s)
      松本正吾、大西敦、本賢一、J.Joe Hull
    • Publisher
      共立出版
    • Related Report
      2008 Final Research Report
  • [Book] 蛋白質 核酸 酵素2007

    • Author(s)
      松本 正吾
    • Total Pages
      6
    • Publisher
      共立出版
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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