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Isolation of the high Al tolerant genes derived from wild plants and application of these genes to heavy metal tolerance in plant

Research Project

Project/Area Number 19580066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University

Principal Investigator

EZAKI Bunichi  Okayama University, 資源生物科学研究所, 准教授 (90243500)

Co-Investigator(Kenkyū-buntansha) NAKASHIMA Susumu  岡山大学, 資源生物科学研究所, 准教授 (60033122)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords野生植物 / 多種ストレス耐性機構 / 重金属ストレス / Alストレス / 酸化ストレス / ABC transporter protein / S-adenosyl methionine sythetase (SAMS) / 耐性遺伝子 / 耐性機構 / ABC transporter like protein / 有用野生植物 / 多種環境ストレス / 金属ストレス / 遺伝子発現応答機構 / メリケンカルカヤ / 有用資源野生植物 / アルミニウムストレス / フィンガープリント法 / 誘導遺伝子
Research Abstract

The aluminum (Al) tolerant mechanism in the two wild plants, Andropogon virginicus L and Miscanthus sinensis, were investigated. ABC transporter gene was isolated as an Al inducible genes from Andropogon. The gene was also response to oxidative stress and heavy metals. Other Andropogon genes which can confer multiple tolerance to various stresses were isolated. One of them was S-adenosyl methionine sysnthetase gene. Finally, we could isolate full length cDNA encoding these two genes from Andropogon.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (33 results)

All 2010 2009 2008 2007 Other

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (17 results) Remarks (2 results)

  • [Journal Article] A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors2010

    • Author(s)
      Kusumadewi Sri Yulita, Takafumi Kouno, Bunichi Ezaki
    • Journal Title

      Electronic Journal of Biotechnology 13

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean2010

    • Author(s)
      Y. Kim, W. Park, H. Nian, T. Sasaki, B. Ezaki,, Y. Jang, G. Chung, H. Bae, S. Ahn
    • Journal Title

      Soil Science and Plant Nutrition 56

      Pages: 140-149

    • NAID

      110008144307

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors2010

    • Author(s)
      Kusumadewi Sri Yulita, Takafumi Kouno, Bunichi Ezaki
    • Journal Title

      Electronic Journal of Biotechnology(インターネット上のopen journalのため、頁番号がない) 13

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean2010

    • Author(s)
      Y.Kim, W.Park, H.Nian, T.Sasaki, B.Ezaki,, Y.Jang, G.Chung, H.Bae, S.Ahn
    • Journal Title

      Soil Science and Plant Nutrition 56

      Pages: 140-149

    • NAID

      110008144307

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of N-terminal His-rich domain of Oscillatoria brevis Bxa1 in both Ag(I)/Cu(I) and Cd(II)/Zn(II) tolerance.2009

    • Author(s)
      E. Nakakihara, H. Kondo H, S. Nakashima, B. Ezaki
    • Journal Title

      The Open Microbiology Journal 3

      Pages: 15-22

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gold induces a metallothionein gene and a CPx-ATPase gene through regulation of a repressor BxmR in Oscillatoria brevis.2009

    • Author(s)
      S. Tagawa, S. Nakashima, B. Ezaki
    • Journal Title

      Japanese Journal of Water Treatment Biology 45

      Pages: 107-113

    • NAID

      10025587219

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of N-terminal His-rich domain of Oscillatoria brevis Bxal in both Ag(I)/Cu(I)and Cd(II)/Zn(II)tolerance2009

    • Author(s)
      E.Nakakihara, H.Kondo H, S.Nakashima, B.Ezaki.
    • Journal Title

      The Open Microbiology Journal 3

      Pages: 15-22

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gold induces a metallothionein gene and a CPx-ATPase gene through regulation of a repressor BxmR in Oscillatoria brevis.2009

    • Author(s)
      S.Tagawa, S.Nakashima, B.Ezaki
    • Journal Title

      Japanese Journal of Water Treatment Biology. 45

      Pages: 107-113

    • NAID

      10025587219

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of N-terminal His-rich domain of Oscillatoria brevis Bxal in both Ag(I)/Cu(I) and Cd(II)/Zn(II) tolerance2009

    • Author(s)
      E. Nakakihara, H. Kondo H, s. Nakashima, B. Ezaki
    • Journal Title

      The Open Microbiology Journal 3

      Pages: 15-22

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Over-expression of an auxilin-like gene (F9E10. 5) can suppress Al uptake in roots of Arabidopsis.2008

    • Author(s)
      B. Ezaki, H. Kiyohara, H. Matsumoto, S. Nakashima
    • Journal Title

      58

      Pages: 497-506

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of high-tolerant wild plants simultaneously to aluminum, heavy metal stresses and oxidative stresses, and characterization of their unique tolerance mechanisms.2008

    • Author(s)
      B. Ezaki, E. Nagao, Y. Yamamoto, S. Nakashima, T. Enomoto
    • Journal Title

      Plant Cell Reports 27

      Pages: 951-961

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Over-expression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis.2008

    • Author(s)
      B. Ezaki. H. Kiyohara, H. Matsumoto, S. Nakashima
    • Journal Title

      J. Exp. Botany 58

      Pages: 497-506

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening of high-tolerant wild plants simultaneously to aluminum, heavy metal stresses and oxidative stresses, and characterization of their unique tolerance mechanisms.2008

    • Author(s)
      B. Ezaki, E. Nagao, Y. Yamamoto, S.Nakashima, T. Enomoto
    • Journal Title

      Plant Cell Reports (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] かび臭物質生産ラン藻Oscillatoria brevisの重金属ストレスに対する適応機構2007

    • Author(s)
      広瀬和信、江崎文一、下石靖昭、村田芳行、中島 進
    • Journal Title

      分取クロマトグラフィー研究会誌 1

      Pages: 16-25

    • Related Report
      2007 Annual Research Report
  • [Presentation] 野生植物Andropogon virginicus L.のAlストレスに応答するABC transporter様遺伝子とSAMS遺伝子の解析2010

    • Author(s)
      高橋憲公, 東藍子, 江崎文一
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本
    • Related Report
      2009 Final Research Report
  • [Presentation] Aluminum and heavy metals stimulate nitric oxide (NO) production in Andropogon virginicus L.2010

    • Author(s)
      Jayaram K, Ezaki B
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本
    • Related Report
      2009 Final Research Report
  • [Presentation] 多種のストレスに応答するAtGST11遺伝子の応答に関わる転写調節因子の単離と解析2010

    • Author(s)
      河野貴文, 江崎文一
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本
    • Related Report
      2009 Final Research Report
  • [Presentation] 野生植物Andropogon virginicus L.のA1ストレスに応答するABC transporter様遺伝子とSAMS遺伝子の解析2010

    • Author(s)
      高橋憲公、東藍子、江崎文一
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] Aluminum and Heavy Metals Stimulate Nitric Oxide(NO)Production in Andropogon virginicus L.2010

    • Author(s)
      Jayaram K, Ezaki B
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多種のストレスに応答するAtGSTll遺伝子の応答に関わる転写調節因子の単離と解析2010

    • Author(s)
      河野貴文、江崎文一
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] 重金属ストレスに対するラン藻Oscillatoria brevisのリプレッサー蛋白BxmRの機能に関する分子遺伝学的解析2009

    • Author(s)
      田川, 中島, 江崎
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 野生植物メリケンカルカヤ(Andropogon virginicus L. )の持つ金属ストレス及び酸化ストレス耐性遺伝子群の単離と解析2009

    • Author(s)
      高橋, 河野, 東, 中島, 江崎
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 金属ストレスや酸化ストレス等の多種のストレスに応答するアラビドプシスのAtGST11遺伝子の応答に関わる転写調節因子の単離と解析2009

    • Author(s)
      河野, Kusumadewi. S. Yulita, 中島, 江崎
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] 金属ストレスや酸化ストレス等の多種のストレスに応答するアラビドプシスのAtGSTll遺伝子の応答に関わる転写調節因子の単離と解析2009

    • Author(s)
      河野, Kusumadewi. S. Yulita, 中島, 江崎
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular genetic characterization for tolerant mechanism and response mechanism against Al stress in plants.2008

    • Author(s)
      B. Ezaki
    • Organizer
      Globalization of Human Resources
    • Place of Presentation
      Gwanjyu, Korea
    • Year and Date
      2008-11-20
    • Related Report
      2009 Final Research Report
  • [Presentation] 糸状体ラン藻に及ぼす重金属の作用の解析2008

    • Author(s)
      中島, 江崎, 山崎, 今野
    • Organizer
      第3回分取クロマトグラフィー研究発表会
    • Place of Presentation
      倉敷
    • Year and Date
      2008-04-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] 野生植物メリケンカルカヤやススキ由来の金属ストレスや酸化ストレス耐性機構および応答機構に関する解析2008

    • Author(s)
      江崎, 高橋, Metwally, 河野, 中木原, 東, 榎本, 中島
    • Organizer
      日本肥料学会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Wild plants including Andropogon virginicus L and Miscanthus sinensis show high tolerance to aluminum heavy metal stresses and oxidative stress by combination of their tolerance mechanism2008

    • Author(s)
      B. Ezaki
    • Place of Presentation
      Banasthali, India
    • Related Report
      2009 Final Research Report
  • [Presentation] 重金属イオンに対するかび臭物質産生ラン藻の応答反応(1)2008

    • Author(s)
      中島, 福田, 橋本, 江崎
    • Organizer
      日本水処理生物学会第45回大会
    • Place of Presentation
      秋田
    • Related Report
      2008 Annual Research Report
  • [Presentation] 重金属イオンに対するかび臭物質産生ラン藻の応答反応(2)2008

    • Author(s)
      福田, 江崎, 中島
    • Organizer
      日本水処理生物学会第45回大会
    • Place of Presentation
      秋田
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular genetic characterization for tolerant mechanism and response mechanism against Al stress in plants.2007

    • Author(s)
      B. Ezaki
    • Organizer
      Globalization of Human Resources 2
    • Place of Presentation
      Gwanjyu, Korea
    • Year and Date
      2007-11-20
    • Related Report
      2007 Annual Research Report
  • [Remarks] 代表者の所属する研究グループのホームページに研究内容に関する記載がある

    • URL

      http://www.rib.okayama-u.ac.jp/sentan/indexj.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.rib.okayama-u.ac.jp/sentan/index-j.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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