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Functional analysis of vesicle transport for nodule formation in Lotus japonicus

Research Project

Project/Area Number 19580068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionKagawa University

Principal Investigator

AGARIE Mika  Kagawa University, 農学部, 准教授 (50294749)

Co-Investigator(Kenkyū-buntansha) TAJIMA Shigeyuki  香川大学, 農学部, 教授 (50116894)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
KeywordsSNARE / ミヤコグサ / 根粒菌 / 共生窒素固定 / 形質転換毛状根 / エンドサイトーシス
Research Abstract

根粒形成、根粒菌細胞内共生について小胞輸送によるオルガネラ形成という観点から小胞輸送の分子レベルでの解明を目的とした。まず、根粒で発現量の増加するSNARE遺伝子を検索した。その結果、GEN06遺伝子は根粒形成に従い根粒で発現量が増加するR-SNAREであることが明らかとなった。特にGen06遺伝子を抑制した毛状根形質転換ミヤコグサの作成を行った結果、毛状根形質転換ミヤコグサは根粒形成が抑制することが明らかとなった。この結果からGen06遺伝子は根粒形成に関与する重要な輸送小胞を担っていると考えられる。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2008 2007

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (18 results)

  • [Journal Article] The 5'-end expressed sequence tags of Lotus japonicus2008

    • Author(s)
      Nomura, M., Tan, D.V., Arunothayanan, H., Asamizu, E., Tabata, S., Kouchi, H. and Tajima, S.
    • Journal Title

      Plant Biotechnology 25

      Pages: 173-175

    • NAID

      10021913054

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzyme Affects Nitrogen Fixing Activity of B. japonicum USDA 110 Bacteroids in Soybean Nodules2008

    • Author(s)
      Dao,T.V., Nomura, M., Hamaguchi, R., Kato, K., Itakura, M., Minamisawa, K., Sinsuwongwat, S., Le, H.T.P, Kaneko, T., Tabata, S., and Tajima, S. NAD-Malic
    • Journal Title

      Microbes and Environment 23, 3

      Pages: 215-220

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway2008

    • Author(s)
      Taketa, S., Amano, S., Tsujino, S., Sato, T., Saisho, D., Kakeda, K., Nomura, M., Suzuki, T., Matsumoto, T., Sato, K., Kanamori, H., Kawasaki, S. and Takeda, K.
    • Journal Title

      Proc. Nat. Acad. Sci. USA. 105

      Pages: 4062-4067

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] NAD-Malic Enzyme Affects Nitrogen Fixing Activity of B. japonicum USDA 110 Bacteroids in Soybean Nodules.2008

    • Author(s)
      Dao, T. V., Nomura, M., Hamaguchi, R., Kato, K., Itakura, M., Minamisawa, K., Sinsuwongwat, S., Le, H. T. P, Kaneko, T., Tabata, S., Tajima, S.
    • Journal Title

      Microbes and Environment 23

      Pages: 215-220

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The 5'-end expressed sequence tags of Lotus japonicus2008

    • Author(s)
      Nomura, M., Tan, D. V., Arunothayanan, H., Asamizu, E., Tabata, S., Kouchi, H., Tajima, S.
    • Journal Title

      Plant Biotechnology 25

      Pages: 173-175

    • NAID

      10021913054

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The 5'-end expressed sequence tags of Lotus japonicus2008

    • Author(s)
      Nomura M., Tan D.V., Arunothayanan H., Asamizu E., Tabata S., Kouchi H.and Tajima S
    • Journal Title

      Plant Biotechnology 25

      Pages: 173-175

    • NAID

      10021913054

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway.2008

    • Author(s)
      Taketa S., Amano S., Tsujino S., Sato T., Saisho D., Kakeda K., Nomura M., Suzuki T., Matsumoto T., Sato K., Kanamori H., Kawasaki S., Takeda K.
    • Journal Title

      Proc.Nat.Acad.Sci.USA 105

      Pages: 4062-4067

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ミヤコグサSNARE遺伝子の機能解析2008

    • Author(s)
      長岡功微菜、古味光紗、真鍋友美、神吉陽輔、浅水絵里香、佐藤修正、田畑哲之、野村美加、田島茂行
    • Organizer
      日本土壌肥料学会関西支部
    • Place of Presentation
      徳島
    • Year and Date
      2008-11-28
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ダイズ・ミヤコグサ根粒バクテロイドプロテオーム解析から見えてくること2008

    • Author(s)
      野村美加、Dao Van Tan, 田島茂行
    • Organizer
      植物微生物研究会
    • Place of Presentation
      奈良
    • Related Report
      2008 Final Research Report
  • [Presentation] ミヤコグサSNARE遺伝子群の機能解析2008

    • Author(s)
      真鍋友美、古味光紗、長岡功微菜、神吉陽輔、浅水絵里香、佐藤修正、田畑哲之、野村美加、田島茂行
    • Organizer
      植物微生物研究会
    • Place of Presentation
      奈良
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Nobel metalloshaperone in the assembly of cytochrome c oxidase of soybean nodule bacteroids2008

    • Author(s)
      Hattahaya Arunothayanan, Tan Van Dao, Rie Hamaguchi, Ayaka Noda, Manabu Itakura, Kiwamu Minamisawa, Mika Nomura, Shigeyuki Tajima
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Screening of Mesorhizobium loti mutants for symbiotic N2 fixation in Lotus japonicus2008

    • Author(s)
      Nanthipak Thapanapongworakul, Tan Van Dao, Kimihiho Toma, Shusei Sato, Yoshikazu Shimoda, Satoshi Tabata, Mika Nomura, Shigeyuki Tajima
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Final Research Report
  • [Presentation] Screening of Mesorhizobium loti mutants for symbiotic N2 fixation in Lotusjaponicus2008

    • Author(s)
      Nanthipak Thapanapongworakul, Tan Van Dao, Kimihiho Toma, Shusei Sato, Yoshikazu Shimoda, Satoshi Tabata, Mika Nomura, Shigeyuki Tajima.
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] obel metalloshaperone in the assembly of cytochrome c oxidase of soybean nodule bacteroids2008

    • Author(s)
      Hattahaya Arunothayanan, Tan Van Dao, Rie Hamaguchi, Ayaka Noda, Manabu Itakura, Kiwamu Minamisawa, Mika Nomura, Shigeyuki Tajima.
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] Proteome analysis of Bradyrhizobium japonicum USDA110 bacteroid differentiation during soybean nodule development2008

    • Author(s)
      野村美加, Dao Van Tan, 田島茂行
    • Organizer
      植物微生物研究会
    • Place of Presentation
      奈良
    • Related Report
      2008 Annual Research Report
  • [Presentation] ミヤコグサ根粒菌との共生に関与するミヤコグサSNARE遺伝子群の検索2007

    • Author(s)
      古味 光紗、真鍋 友美、長岡 功微菜、浅水 絵里香、佐藤 修正、田畑 哲、竹川 薫、野村 美加、田島 茂行
    • Organizer
      植物微生物研究会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2007-09-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] 共生窒素固定に関与するダイズ根粒菌(Bradyrhizobium japonicumUSDA110)バクテロイドタンパク質の検索2007

    • Author(s)
      野田 朱花、DAO VAN TAN、浜口 理恵、加藤 賢祐、南澤 究、板倉 学、野村 美加、田島 茂行
    • Organizer
      植物微生物研究会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2007-09-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] Characterization of mitochondrial proteins from soybean and Lotus japonicus nodules2007

    • Author(s)
      Hattahaya Arunothayanan, Mika Nomura, Shigeyuki Tajima
    • Organizer
      植物微生物研究会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2007-09-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] PEPC酵素を抑制した形質転換ミヤコグサ根粒における代謝産物の解析2007

    • Author(s)
      上田 知幸, 藤井 美帆, 野村 美加, 田島 茂行
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2007-08-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] Proteome analysis of Bradyrhizobium japonicum USDA110 bacteroid differentiation during soybean nodule development2007

    • Author(s)
      Dao Van Tan, Le Phuong Hoa, Mika Nomura, Kazuhika Saeki, Kiwamu Minamisawa, Takakazu Kaneko, Satoshi Tabata, Shigeyuki Tajima
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2007-08-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] 共生窒素固定に関与するダイズ根粒菌(Bradyrhizobium japonicum USDA110)バクテロイドタンパク質の検索2007

    • Author(s)
      野田朱花、DAO VAN TAN、濱口理恵、加藤賢祐、南澤究、板倉学、野村美加、田島茂行
    • Organizer
      植物微生物研究会
    • Place of Presentation
      鹿児島
    • Related Report
      2008 Final Research Report
  • [Presentation] Characterization of mitochondrial proteins from soybean and Lotus japonicus nodules2007

    • Author(s)
      Hattahaya Arunothayanan, Mika Nomura, Shigeyuki Tajima
    • Organizer
      植物微生物研究会
    • Place of Presentation
      鹿児島
    • Related Report
      2008 Final Research Report
  • [Presentation] PEPC酵素を抑制した形質転換ミヤコグサ根粒における代謝産物の解析2007

    • Author(s)
      上田知幸・藤井美帆・野村美加・田島茂行
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] Proteome analysis of Bradyrhizobium japonicum USDA110 bacteroid differentiation during soybean nodule development2007

    • Author(s)
      Dao Van Tan, Le Phuong Hoa, Mika Nomura, Kazuhiko Saeki, Kiwamu Minamisawa, Takakazu Kaneko,Satoshi Tabata , Shigeyuki Tajima,
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] ミヤコグサ根粒菌との共生に関与するミヤコグサSNARE遺伝子群の検索2007

    • Author(s)
      古味光紗、真鍋友美、長岡功微菜、浅水絵里香、佐藤修正、田畑哲之、竹川薫、野村美加、田島茂行
    • Organizer
      植物微生物研究会
    • Place of Presentation
      鹿児島
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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