Mechanism of biosynthesis for circular antibacterial peptides produced by lactic acid bacteria from human intestines

• Project/Area Number: 19580076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tohoku University
• Principal Investigator: KAWAI Yasushi Tohoku University, 大学院・農学研究科, 助教 (00261496)
• Co-Investigator(Kenkyū-buntansha): SAITO Tadao 東北大学, 大学院・農学研究科, 教授 (00118358) ; KITAZAWA Haruki 東北大学, 大学院・農学研究科, 准教授 (10204885) ; NISHIMURA Jyunko 東北大学, 大学院・農学研究科, 技術専門職員 (10241556)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 微生物機能 / バクテリオシン / 乳酸菌 / Lactobacillus gasseri / D-アミノ酸 / 環状ペプチド

Research Abstract

ヒトの腸管に住んでいる安全性の高い乳酸菌は、抗菌物質を生産し、整腸作用や食品保存に貢献している。本研究では、乳酸菌によって作られるタンパク質性の抗菌物質(バクテリオシン)に着目し、熱や酸に安定性を与えるタンパク質(通常は一本の鎖状で存在している)のリング(環状)構造形成に関わる因子(遺伝子)を特定することに成功した。本成果は、世界で初めての事例であり、現在、論文を作成中である。

Research Products

• [Journal Article] Negative effect of divalent metal cations on production of gassericin T a bacteriocin produced by Lactobacillus gasseri, in milk-based media (2009)
  • Author(s): Kensuke Arakawa, Yasushi Kawai, Junko Nishimura, Haruki Kitazawa and Tadao Saito
  • Journal Title: International Dairy Journal(in press)
  • Peer Reviewed
• [Journal Article] Effects of gassericins A and T, bacteriocins produced by Lactobacillus gasseri, with glycine on custard cream preservation (2009)
  • Author(s): Kensuke Arakawa, Yasushi Kawai, Hiroyuki Iioka, Masashi Tanioka, Junko Nishimura, Haruki Kitazawa, Koichi Tsurumi and Tadao Saito
  • Journal Title: Journal of Dairy Science 92(6) Pages: 2365-2372
  • Peer Reviewed
• [Journal Article] DNA sequencing and homologous expression of a small peptide conferring immunity to gassericin A, a circular bacteriocin produced by Lactobacillus gasseri LA39 (2009)
  • Author(s): Yasushi Kawai, Joni Kusnadi, Rober Kemperman, Jan Kok, Yoshiyuki Ito, Mikiko Endo, Kensuke Arakawa, Hideaki Uchida, Junko Nishimura, Haruki Kitazawa and Tadao Saito
  • Journal Title: Applied and Environmental Microbiology 75(5) Pages: 1324-1330
  • Peer Reviewed
• [Journal Article] DNA-sequencing and homologous expression of a small peptide conferring immunity to gassericin A, a circular bacteriocin produced by Lactobacillus gasseri LA39 (2009)
  • Author(s): Y. Kawai, et.al.
  • Journal Title: Applied and Environmental Microbiology 75(5) Pages: 1324-1330
  • Peer Reviewed
• [Journal Article] Effects of gassericins A and T, bacteriocins produced by Lactobacillus gasseri, with glycine on custard cream preservation (2009)
  • Author(s): K. Arakawa, Y. Kawai, et.al.
  • Journal Title: Journal of Dairy Science (in press)
  • Peer Reviewed
• [Journal Article] Bacteriocin production of probiotic Lactobacillus gasseri LA39 isolated from human feces in milk-based media (2008)
  • Author(s): Kensuke Arakawa, Yasushi Kawai, Kenji Fujitani, Junko Nishimura, Haruki Kitazawa, Ken-ichi Komine, Kenzo Kai and Tadao Saito
  • Journal Title: Animal Science Journal 79(5) Pages: 634-640
  • NAID: 10025370789
  • Peer Reviewed
• [Journal Article] Microbial community analysis of food-spoilage bacteria in commercial custard creams using culture-dependent and -independent methods (2008)
  • Author(s): Kensuke Arakawa, Yasushi Kawai, Hiroyuki Iioka, Masashi Tanioka, Junko Nishimura, Haruki Kitazawa, Koichi Tsurumi and Tadao Saito
  • Journal Title: Journal of Dairy Science 91(8) Pages: 2938-2946
  • Peer Reviewed
• [Journal Article] Microbial community analysis of food-spoilage bacteria in commercial custard creams using culture-dependent and -independent methods. (2008)
  • Author(s): K. Arakawa, Y. Kawai*, et. al.
  • Journal Title: Journal of Dairy Science (in press)
  • Peer Reviewed
• [Journal Article] Bacteriocin production of probiotic Lactobacillus gasseri LA39 isolated from human feces in milk-based media. (2008)
  • Author(s): K. Arakawa, Y. Kawai*, et. al.
  • Journal Title: Animal Science Journal (in press)
  • NAID: 10025370789
  • Peer Reviewed
• [Presentation] Lactobacillus gasseriが生産する抗菌ペプチドを用いたカスタードクリームの新規保存法の開発 (2009)
  • Author(s): 荒川健佑、川井泰ら
  • Organizer: 日本畜産学会大110回(2008年度)大会
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-27
• [Presentation] バクテリオシン(ガセリシンA)に対する乳酸菌の自己耐性因子とその発現解析 (2009)
  • Author(s): 川井泰ら
  • Organizer: 日本畜産学会大110回(2008年度)大会
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-27
• [Presentation] Lacotobacillus gasseriが力宝生産する抗菌ペプチドを用いたカスタードクリームの新規保存法の開発 (2009)
  • Author(s): 荒川健佑、川井泰, ら
  • Organizer: 日本畜産学会大110回(2008年度)大会
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-27
• [Presentation] 2成分性のバクテリオシン(ガセリシンT)の生合成遺伝子とタンパク質化学的特性 (2009)
  • Author(s): 中條貴弘、川井泰, ら
  • Organizer: 日本畜産学会大110回(2008年度)大会
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-27
• [Presentation] IMPROVED GROWTH AND INCREASED BACTERIOCIN PRODUCTION OF PROBIOTIC Lactobacillus gasseri STRAINS IN MILK-BASED MEDIA (2008)
  • Author(s): K. Arakawa, Y. Kawai, et.al.
  • Organizer: 9th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Egmond aan Zee (The Netherlands)
  • Year and Date: 2008-09-01
• [Presentation] EFFECT OF FATTY ACIDS (C18 : 1, OCTADECENOIC ACIDS) TO BACTERIOCIN PRODUCTION AND RESISTANCE OF Lactobacillus gasseri STRAINS ISOLATED FROM HUMAN INTESTINE AND FECES (2008)
  • Author(s): Y. Kawai, et.al.
  • Organizer: 9th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Egmond aan Zee (The Netherlands)
  • Year and Date: 2008-09-01
• [Presentation] EFFECT OF FATTY ACIDS (C18:1, OCTADECENOIC ACIDS) TO BACTERIOCIN PRODUCTION AND RESISTANCE OF Lactobacillus gasseri STRAINS ISOLATED FROM HUMAN INTESTINE AND FECES (2008)
  • Author(s): Y. Kawai, et.al.
  • Organizer: 9th Symposium on Lactic Acid Bacteria The Netherlands
  • Place of Presentation: Egmond aan Zee(Hotel Zuiderduin)オランダ
  • Year and Date: 2008-09-01
• [Presentation] IMPROVED GROWTH AND INCREASED BACTERIOCIN PRODUCTION OF PROBIOTIC Lactobacillus gasseri STRAINS IN MILK-BASED MEDIA (2008)
  • Author(s): K. Arakawa, Y. Kawai, et.al.
  • Organizer: 9th Symposium on Lactic Acid Bacteria The Netherlands
  • Place of Presentation: Egmond aan Zee オランダ (Hotel Zuiderduin)
  • Year and Date: 2008-09-01
• [Presentation] ヒト由来乳酸桿菌のバクテリオシン生産性と耐性に脂肪酸(オクタデセン酸、C18 : 1)が及ぼす影響 (2008)
  • Author(s): 伊藤円香、川井泰ら
  • Organizer: 日本乳酸菌学会2008年度大会
  • Place of Presentation: 京都大学
  • Year and Date: 2008-07-14
• [Presentation] ヒト由来乳酸桿菌のバクテリオシン生産性と耐性に脂肪酸(オクタデセン酸、C18:1)が及ぼす影響 (2008)
  • Author(s): 伊藤円香、川井泰, ら
  • Organizer: 日本乳菌学会2008年度大会
  • Place of Presentation: 京都大学
  • Year and Date: 2008-07-14
• [Presentation] ガセリシンTの生産性に及ぼす二価金属イオンの影響 (2008)
  • Author(s): 荒川健佑、川井泰ら
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-28
• [Presentation] 乳酸桿菌のバクテリオシン生産性と耐性はオクタデセン酸により変化する (2008)
  • Author(s): 伊藤円香, 川井泰ら
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-27
• [Presentation] 市販笹かまぼこから分離した各種細菌の同定 (2008)
  • Author(s): 石川、川井, ら
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名城大学
  • Year and Date: 2008-03-27
• [Presentation] Application of bacteriocin produced by probiotic Lactobacillus gasseri LA39 to biopreservation of custard cream (2007)
  • Author(s): K. Arakawa, Y. Kawai, et.al.
  • Organizer: The 4th Asian Conference on Lactic Acid Bacteria & the 3rd International Symposium on Lactic Acid Bacteria and Health
  • Place of Presentation: Shanghai Everbright Conve-ntion & Exhibition Centre International Hotel
  • Year and Date: 2007-10-15
• [Presentation] Characteristics of bacteriocins and their producing Lactobacillus gasseri strains isolated from human infant feces (2007)
  • Author(s): Y. Kawai, et.al.
  • Organizer: The 4th Asian Conference on Lactic Acid Bacteria & the 3rd International Symposium on Lactic Acid Bacteria and Health
  • Place of Presentation: Shanghai Everbright Conve-ntion & Exhibition Centre International Hotel.
  • Year and Date: 2007-10-15
• [Presentation] Characteristics of bacteriocins and their producing Lactobacillus gasseri strains isolated from human infant feces. (2007)
  • Author(s): ○Y. Kawai, et. al.
  • Organizer: The 4th Asian Conference on Lactic Acid Bacteria & the 3rd International Symposium on Lactic Acid Bacteria and Health
  • Place of Presentation: Shanghai Everbright Convention & Exhibition Centre International Hotel
  • Year and Date: 2007-10-15
• [Presentation] Application of bacteriocin produced by probiotic Lactobacillus gasseri LA39 to biopreservation of custard cream. (2007)
  • Author(s): K. Arakawa, Y. Kawai, et. al.
  • Organizer: The 4th Asian Conference on Lactic Acid Bacteria & the 3rd International Symposium on Lactic Acid Bacteria and Health
  • Place of Presentation: Shanghai Everbright Conve-ntion & Exhibition Centre International Hotel
  • Year and Date: 2007-10-15
• [Presentation] ヒト由来乳酸桿菌のバクテリオシン生産性と耐性に脂肪酸(オクタデセン酸、C18:1)が及ぼす影響 (2007)
  • Author(s): 伊藤、○川井, ら
  • Organizer: 日本乳酸菌学会2007年度大会
  • Place of Presentation: 麻布大学
  • Year and Date: 2007-07-05
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/douka/index-j.html
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/douka/index-j.html
• [Patent(Industrial Property Rights)] ガセリシンAの生産方法及び食品保存剤 (2009)
  • Inventor(s): 齋藤忠夫, 川井泰, 荒川健佑, 飯岡宏之, 谷岡政司, 鶴見幸市
  • Industrial Property Rights Holder: 東北大学, 月島食品工業(株)
  • Patent Publication Number: 2009-044983
  • Filing Date: 2009-03-05
• [Patent(Industrial Property Rights)] 特願 (2007)
  • Inventor(s): 川井, ら
  • Industrial Property Rights Holder: 東北大学
  • Filing Date: 2007-08-16