Analysis of the novel promoter sequences recognized by the group 3 sigma factor in cyanobacteria

• Project/Area Number: 19580078
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Ibaraki University
• Principal Investigator: ASAYAMA Munehiko Ibaraki University, 農学部, 准教授 (50231907)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 光合成微生物 / シアノバクテリア(藍藻) / 転写制御 / RNAポリメラーゼ / シグマ因子 / シアノバクテリア / 転写 / RNA polymerase / sigF / pilA / pixJ / プロモーター / SigF

Research Abstract

This project focused on SigF belonging to the type 3 sigma factor for RNA polymerase of the cyanobacterium Synechocystis sp. strain PCC6803 as a model photosynthesizing organism and promoter-recognition ability of SigF was verified. The results revealed a novel and common core promoter-sequence with TAGGC (-32 region) and GGTAA (-12 region) as important for transcription. We discuss a model case of stringent promoter recognition by SigF and promoter types of PCC6803 genes are also summarized.

Research Products

• [Journal Article] Sigma Factors for Cyanobacterial Transcription (2009)
  • Author(s): Imamura S, Asayama M
  • Journal Title: Gene Regulation and Systems Biology 3 Pages: 65-87
  • Peer Reviewed
• [Journal Article] Stringent promoter recognition and autoregulation by the group 3 σ-factor SigF in the cyanobacterium Synechocystis sp. strain PCC 6803 (2008)
  • Author(s): Asayama M, Imamura S
  • Journal Title: Nucleic Acids Research 16 Pages: 5297-5305
  • Peer Reviewed
• [Journal Article] Stringent promoter recognition and autoregulation by the group 3 σ-factor SigF in the cyanobacterium Synechocystis sp. strain PCC 6803 (2008)
  • Author(s): Asayama M and Imamura S
  • Journal Title: Nucleic Acids Research 16 Pages: 5297-5305
  • Peer Reviewed
• [Presentation] Interference Expression at Levels of the Transcript and Protein among Group 1, 2, and 3 Sigma Factor Genes in a Cyanobacterium (2009)
  • Author(s): 朝山宗彦
  • Organizer: 第30回日本分子生物学会 (2007年度) 年会・第80回日本生化学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] Construction and Utilization of Cyanobacterial Expression Vector (2009)
  • Author(s): ASAYAMA Munehiko, HIRANO Eriko, HANAMI Tomoyo, HORIE Yoshinao, NISHIZAWA Tomoyasu, SHIRAI Makoto
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] 高効率プロモーターを有する藻発現ベクターの構築と利用 (2009)
  • Author(s): 朝山宗彦, 平野恵理子, 花見知世, 堀江良尚, 西澤智康, 白井誠
  • Organizer: ラン藻の分子生物学2009 (CYANO 2009)
  • Place of Presentation: かずさアカデミアホール
  • Year and Date: 2009-12-05
• [Presentation] 高効率プロモーターを有する藻発現ベクターの構築と利用 (2009)
  • Author(s): 朝山宗彦、平野恵理子、花見知世、堀江良尚、西澤智康、白井誠
  • Organizer: ラン藻の分子生物学2009(CYANO 2009)
  • Place of Presentation: かずさアカデミアホール
  • Year and Date: 2009-12-05
• [Presentation] シグマ因子とRNaseE相互作用因子 : 転写・転写後調節のこれまでのまとめと今後 (2009)
  • Author(s): 朝山宗彦
  • Organizer: ラン藻ゲノム交流会
  • Place of Presentation: 東京大学駒場キャンパス16号館
  • Year and Date: 2009-07-04
• [Presentation] シグマ因子とRNaseE相互作用因子:(サブタイトル)転写・転写後調節のこれまでのまとめと今後 (2009)
  • Author(s): 朝山宗彦
  • Organizer: ラン藻ゲノム交流会
  • Place of Presentation: 東京大学教養部16号館126/237講義室
  • Year and Date: 2009-07-04
• [Presentation] Stringent promoter recognition and autoregulation by the group 3 sigma-factor SigF in the cyanobacterium Synechocystis sp. strain PCC 6803 (2008)
  • Author(s): 朝山宗彦
  • Organizer: 第31回日本分子生物学会 (2008年度) 年会・第81回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] Stringent promoter recognition and autoregulation by the group 3 sigma-factor SigF in the cyanobacterium Synechocystis sp. strain PCC 6803 (2008)
  • Author(s): 朝山宗彦
  • Organizer: 第31回日本分子生物学会(2008年度)年会・第81回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] Interference Expression at Levels of the Transcript and Protein among Group 1, 2, and 3 Sigma Factor Genes in a Cyanobacterium (2007)
  • Author(s): 朝山宗彦
  • Organizer: 第30回日本分子生物学会(2007年度)年会・第80回日本生化学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Remarks] ホームページ
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm
• [Remarks]
  • URL: http://asam.agr.ibaraki.ac.jp/
• [Remarks]
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm
• [Remarks] 茨城大学研究者情報総覧
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm
• [Remarks] 茨城大学研究者情報総覧
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm