Elucidation of degrading processes of polyunsaturated fatty acids by introducing a gene encoding polyhydroxyalkanoate synthase

• Project/Area Number: 19580080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Utsunomiya University
• Principal Investigator: MAEDA Isamu Utsunomiya University, 農学部, 准教授 (10252701)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2008: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2007: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 不飽和脂肪酸 / ポリヒドロキシアルカン酸 / オレイン酸 / リノール酸 / リノレン酸 / 酵母 / Saccharomyces cerevisiae / β-酸化 / Saccharomyces cervisiae

Research Abstract

When a polyunsaturated fatty acid contains the 12-cis double bond, dienoyl-CoA reductase activity has been shown to play an important role in β-oxidation of the fatty acid. As both linoleic acid and α-linolenic acid contain the 9-cis and 12-cis double bonds, it was suggested in α-linolenic acid that its inhibitory effect on β-oxidation and the higher necessity of dienoyl-CoA reductase activity in its metabolism might be due to the existence of 15-cis double bond.

Research Products

• [Journal Article] Genetic replacement of tesB with PTE1 affects chain-length proportions of 3-hydroxyalkanoic acids produced through β-oxidation of oleic acid in Escherichia coli (2010)
  • Author(s): Yoshiaki Seto, Junkyu Kang, Li Ming, Naoto Habu, Ken-ichi Nihei, Shunsaku Ueda, Isamu Maeda
  • Journal Title: Journal of Bioscience and Bioengineering (in press)
  • NAID: 110007880134
  • Peer Reviewed
• [Journal Article] Genetic replacement of tesB with PTE1 affects chain-length proportions of 3-hydroxyalkanoic acids produced through β-oxidation of oleic acid in Escherichia coli (2010)
  • Author(s): Yoshiaki Seto, Junkyu Kang, Li Ming, Naoto Habu, Ken-ichi Nihei, Shunsaku Ueda, Isamu Maeda
  • Journal Title: Journal of Bioscience and Bioengineering (印刷中)
  • NAID: 110007880134
  • Peer Reviewed
• [Presentation] 出芽酵母を用いた多価不飽和脂肪酸β-酸化過程の解明 (2009)
  • Author(s): 高浜由実, 上田俊策, 前田勇
  • Organizer: 日本農芸化学会関東支部2009年度大会
  • Place of Presentation: 東京都町田市 玉川大学視聴覚センター
  • Year and Date: 2009-10-31
• [Presentation] 出芽酵母を用いた多価不飽和脂肪酸β-酸化過程の解明 (2009)
  • Author(s): 高浜由実、上田俊策、前田勇
  • Organizer: 日本農芸化学会関東支部2009年度大会
  • Place of Presentation: 玉川大学 視聴覚センター
  • Year and Date: 2009-10-31
• [Presentation] アシル-CoAチオエステラーゼII (TesB) ファミリー酵素のβ-酸化中間体に対する基質特異性の解析 (2008)
  • Author(s): 瀬戸慶彬, 長谷川晴子, 上田俊策, 前田勇
  • Organizer: 日本農芸化学会関東支部2007年度大会
  • Place of Presentation: 宇都宮市 宇都宮大学大学会館多目的ホール
  • Year and Date: 2008-11-10
• [Presentation] acyl-CoA thioesterase II (TesB) family 酵素のβ-酸化中間体に対する基質特異性の解析 (2008)
  • Author(s): 瀬戸慶彬, 長谷川晴子, 上田俊策, 前田勇
  • Organizer: 第59回日本生物工学会大会
  • Place of Presentation: 東広島市 広島大学東広島キャンパス
  • Year and Date: 2008-09-26
• [Presentation] acyl-CoA thioesterase II(TesB)family酵素のβ-酸化中間体に対する基質特異性の解析 (2007)
  • Author(s): 瀬戸 慶彬, 長谷川 清子, 上田 俊策, 前田 勇
  • Organizer: 第59回日本生物工学会大会
  • Place of Presentation: 東広島市、広島大学東広島キャンパス
  • Year and Date: 2007-09-26
• [Remarks] ホームページ等
  • URL: http://agri.mine.utsunomiya-u.ac.jp/hpj/deptj/chemj/jmicrobio/microb-eng.html
• [Remarks]
  • URL: http://agri.mine.utsunomiya-u.ac.jp/hpj/deptj/chemj/jmicrobio/microb-eng.html
• [Remarks]
  • URL: http://agri.mine.utsunomiya-u.ac.jp/hpj/deptj/chemj/jmicrobio/microb-eng.html