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Comparative functional analysis based on the 3D-structures of plant and microbial family 19 chitinases

Research Project

Project/Area Number 19580084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionNiigata University

Principal Investigator

WATANABE Takeshi  Niigata University, 自然科学系, 教授 (10201203)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords微生物機能 / キチナーゼ / ファミリー19 / 活性ドメイン / キチン吸着ドメイン / イネ / 放線菌 / ループ構造
Research Abstract

イネクラスI キチナーゼCht2からループIIを削除した欠失変異キチナーゼを構築し、野生型Cht2とその性質を比較した結果、ループIIが+3サブサイトを形成していることが明らかとなった。また、ループIIは顕著な生理活性を持つ4糖以上の長鎖キチンオリゴ糖の生成に重要な役割を果たしていることがわかり、植物クラスIキチナーゼがキチンエリシター産生において中心的役割を果たしていることが強く示唆された。また、放線菌ファミリー19キチナーゼChiCのキチン吸着には、吸着に重要であることがわかっているTrp59とTrp60に加えて、その周辺に存在するThr43, Glu64, Gln62, Asp70が関与していることが明らかとなった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (43 results)

All 2009 2008 2007 Other

All Journal Article (17 results) (of which Peer Reviewed: 15 results) Presentation (21 results) Book (4 results) Remarks (1 results)

  • [Journal Article] The importance of chitobiase and N-acetylglucosamine(GlcNAc)uptake in N,N'-diacetylchitobiose[(GlcNAc)2]utilization by Serratia marcescens 21702008

    • Author(s)
      T. Toratani, T. Shoji, T. Ikehara, K. Suzuki, and T. Watanabe
    • Journal Title

      Microbiology 154

      Pages: 487-495

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of the loop structure of the catalytic domain in rice class I chitinase2008

    • Author(s)
      R. Mizuno, T. Fukamizo, S. Sugiyama, Y. Nishizawa, Y. Kezuka, T. Nonaka, K. Suzuki and T. Watanabe
    • Journal Title

      J. Biochem 143

      Pages: 893-895

    • NAID

      10024909297

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of recombinant chitinase from E. coli carrying the cDNAof family 19 chitinase from rice(Oryza sativa L.)2008

    • Author(s)
      R. Mizuno, Y. Itoh, Y. Nishizawa, K. Suzuki, and T. Watanabe
    • Journal Title

      Biosci. Biotechnol. Biochem 72

      Pages: 893-895

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chitinase 3-like-1 enhances bacterial adhesion to colonic epithelial cells through the interaction with bact erial chitin-binding protein2008

    • Author(s)
      M. Kawada, C.-C. Chen, A. Arihiro, K. Nagatani, T. Watanabe and E. Mizoguchi
    • Journal Title

      Lab. Invest 88

      Pages: 883-895

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Takahashi, S. Yanagi and H. Kihara, SaxsMDView : a three-dimensional graphics program for displaying force vectors2008

    • Author(s)
      M. Kojima, Y. Kezuka, T. Nonaka, Y. Hiragi T. Watanabe, K. Kimura, K. Takahashi, S. Yanagi and H. Kihara
    • Journal Title

      Journal of Synchrotron Radiation 15

      Pages: 535-537

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phage display screening for peptidic chitinase inhibitors2008

    • Author(s)
      C. Peter, C. Scholz, H. Wessner, G. Hansen, P. Henklein, T. Watanabe and W. Hohne
    • Journal Title

      J. Mol. Recogn 21

      Pages: 401-409

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chitinase inhibitor allosamidin promotes chitinase production of Streptomyces generally2008

    • Author(s)
      S. Suzuki, E. Nakanishi, K. Furihata, K. Miyamoto, H. Tsujibo, T. Watanabe, Y. Ohnishi, S. Horinouchi, H. Nagasawa, S. Sakuda
    • Journal Title

      Int. J. Biol. Macromol 43

      Pages: 13-19

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of exposed tryptophan and tyrosine residues on the surface of chitinase B from Serratia marcescens 21702008

    • Author(s)
      M. Muto, A. Ohara, K. Azuma, K. Suzuki, and T. Wat anabe
    • Journal Title

      Proceeding of MIE BIOFORUM 2008

    • Related Report
      2008 Final Research Report
  • [Journal Article] SaxsMDView : a three-dimensional graphics program for displaying force vectors.2008

    • Author(s)
      M. Kojima, T. Watanabe, 他
    • Journal Title

      Journal of Synchrotron Radiation. 15

      Pages: 535-537

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phage display screening for peptidic chitinase inhibitors.2008

    • Author(s)
      C. Peter, T. Watanabe, 他
    • Journal Title

      Journal of Molecular Recognition 21

      Pages: 401-409

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chitinase 3-like-1 enhances bacterial adhesion to colonic epithelial cells through the interaction with bacterial chitin-binding protein.2008

    • Author(s)
      M. Kawada, T. Watanabe, 他
    • Journal Title

      Laboratory Investigation 88

      Pages: 883-895

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chitinase inhibitor allosamidin promotes chitinase production of Streptomyces generally.2008

    • Author(s)
      S. Suzuki, T. Watanabe, 他
    • Journal Title

      International Journal of Biological Macromolecules 43

      Pages: 13-19

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of exposed tryptophan and tyrosine residues on the surface of chitinase B from Serratia marcescens 2170.2008

    • Author(s)
      M. Muto, T. Watanabe, 他
    • Journal Title

      Proceeding of MIE BIOFORUM 2008. (CD-ROM)

    • Related Report
      2008 Annual Research Report
  • [Journal Article] The importance of chitobiase and N-acetylglucosamine (GlcNAc) uptake in N,N'-diacetylchitobiose [(GlcNAc)2] utilization by Serratia marcescens 2170.2008

    • Author(s)
      T. Toratani, T. Watanabe, 他
    • Journal Title

      Microbiology 154

      Pages: 1326-1332

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of the loop structure of the catalytic domain in rice class I chitinase.2008

    • Author(s)
      R. Mizuno, T. Watanabe, 他
    • Journal Title

      J. Biochem. 143

      Pages: 487-495

    • NAID

      10024909297

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of recombinant chitinase from E. coli carrying the cDNA of family 19 chitinase from rice (Oryza sativa L.).2008

    • Author(s)
      R. Mizuno, T. Watanabe, 他
    • Journal Title

      Biosci. Biotechnol. Biochem. 72

      Pages: 893-895

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chitinase 3-like-1 enhances bacterial adhesion to colonic epithelial cells through the interaction with bacterial chitin-binding protein.2008

    • Author(s)
      M. Kawada, T. Watanabe, 他
    • Journal Title

      Lab. Invest. (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 転写因子chiRを破壊したSerratiamarcescensのプロテオーム解析2008

    • Author(s)
      庄司俊裕・虎谷忠幸・宮本勝城・辻坊裕・鈴木一史・渡邉剛志
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋市
    • Related Report
      2008 Final Research Report
  • [Presentation] Serratia marcescens 2170由来キチナーゼBの表面に露出したトリプトファンおよびチロシン残基の機能2008

    • Author(s)
      武藤亜紀子・大原麻子・吾妻香織・鈴木一史・渡邉剛志
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋市
    • Related Report
      2008 Final Research Report
  • [Presentation] Serratia marcescensのキチナーゼ発現におけるybfM 5'-UTRの重要性2008

    • Author(s)
      虎谷忠幸・鈴木一史・渡邉剛志
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋市
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Streptomyces griseus HUT6037由来キチナーゼCの構造と機能の解明2008

    • Author(s)
      吉田優・菅丈司・水野亮二・毛塚雄一郎・野中孝昌・池上貴久・鈴木一史・渡邉剛志
    • Organizer
      第22回キチン・キトサンシンポジウム
    • Place of Presentation
      新潟市コンベンションセンター
    • Related Report
      2008 Final Research Report
  • [Presentation] Serratiamarcescens 2170由来キチナーゼBの表面に露出したトリプトファン及びチロシン残基の機能2008

    • Author(s)
      武藤亜紀子・大原麻子・吾妻香織・鈴木一史・渡邉剛志
    • Organizer
      第22回キチン・キトサンシンポジウム
    • Place of Presentation
      新潟市コンベンションセンター
    • Related Report
      2008 Final Research Report
  • [Presentation] Serratia marcescensのキチナーゼ発現におけるybfM 5'-UTRの重要性2008

    • Author(s)
      虎谷忠幸・山内友恵・鈴木一史・渡邉剛志
    • Organizer
      第22回キチン・キトサンシンポジウム
    • Place of Presentation
      新潟市コンベンションセンター
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Streptomycesgriseus HUT6037由来キチナーゼCの構造と機能の解明2008

    • Author(s)
      吉田優・水野亮二・菅丈司・毛塚雄一郎・野中孝昌・池上貴久・鈴木一史・渡邉剛志
    • Organizer
      第22回キチン・キトサンシンポジウム
    • Place of Presentation
      新潟市コンベンションセンター
    • Related Report
      2008 Final Research Report
  • [Presentation] イネクラスIキチナーゼ活性ドメインのループIIの機能解析2008

    • Author(s)
      杉山真一・水野亮二・西澤洋子・深溝慶・毛塚雄一郎・野中孝昌・鈴木一史・渡邉剛志
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸ポートピアアイランド
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] The role of exposed tryptophan and tyrosine residues on the surface of chitinase B from Serratia marcescens 21702008

    • Author(s)
      M. Mut o, A. Ohara, K. Azuma, K. Suzuki, and T. Wat anabe
    • Organizer
      MIE BIOFORUM
    • Place of Presentation
      三重県志摩市
    • Related Report
      2008 Final Research Report
  • [Presentation] Serratia marcescens 2170由来キチナーゼBの表面に露出したトリプトファン及びチロシン残基の機能2008

    • Author(s)
      武藤亜紀子, 渡邉剛志, 他
    • Organizer
      第22回キチン・キトサンシンポジウム
    • Place of Presentation
      新潟市コンベンションセンター
    • Related Report
      2008 Annual Research Report
  • [Presentation] Streptomyces griseus HUT6037 由来キチナーゼCの構造と機能の解明2008

    • Author(s)
      吉田優, 渡邉剛志, 他
    • Organizer
      第22回キチン・キトサンシンポジウム
    • Place of Presentation
      新潟市コンベンショーンセンター
    • Related Report
      2008 Annual Research Report
  • [Presentation] The role of exposed tryptophan and tyrosine residues on the surface of chitinase B from Serratia marcescens 21702008

    • Author(s)
      杉山真一, 渡邉剛志, 他
    • Organizer
      MIE BIOFORUM 2008
    • Place of Presentation
      三重県志摩市
    • Related Report
      2008 Annual Research Report
  • [Presentation] 転写因子chiRを破壊したSerratia marcescensのプロテオーム解析2008

    • Author(s)
      庄司俊裕、渡邉剛志, 他
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Serratia marcescens2170由来キチナーゼBの表面に露出したトリプトファンおよびチロシン残基の機能2008

    • Author(s)
      武藤亜紀子、渡邉剛志, 他
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Streptomyces griseus HUT6037由来キチナーゼCの構造と機能の解明2008

    • Author(s)
      吉田優、渡邉剛志, 他
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Structure and function of family 19 chitinases2007

    • Author(s)
      T. Watanabe, Y. Kezuka, T. Nonaka, T. Ikegami, Y. Nishizawa, M. Mitsutomi, and T. Fukamizo
    • Organizer
      8^<th>International Conference of the European ChitinSociety
    • Place of Presentation
      トルコアンタルア
    • Related Report
      2008 Final Research Report
  • [Presentation] Antifungal chitinases : structure, functi on and possible application to biocontrol2007

    • Author(s)
      T. Watanabe, R. Mizuno, K. Suzuki, Y. Kezuka, T. Nonaka, And Y. Nishizawa
    • Organizer
      1^<st>international meeting for developme nt of IPM in Asia and Afri ca
    • Place of Presentation
      タイチェンマイ
    • Related Report
      2008 Final Research Report
  • [Presentation] Serratia marcescens2170のキチン分解利用に関わるオペロンの機能解析2007

    • Author(s)
      虎谷忠幸・庄司俊裕・鈴木一史・渡邉剛志
    • Organizer
      第21回キチン・キトサンシンポジウム
    • Place of Presentation
      福井市
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Vibrio fisheri MKT20由来ファミリー19キチナーゼの機能解析2007

    • Author(s)
      水野亮二・大橋大輔・鈴木一史・渡邉剛志
    • Organizer
      第21回キチン・キトサンシンポジウム
    • Place of Presentation
      福井市
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Structure and function of family 19 chitinases.2007

    • Author(s)
      T. Watanabe, 他
    • Organizer
      8th International Conference of the European Chitin Society
    • Place of Presentation
      アンタルヤ(トルコ)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Antifungal chitinases: structure, function and possible application to biocontrol.2007

    • Author(s)
      T. Watanabe, 他
    • Organizer
      1st international meeting for development of IPM in Asia and Africa
    • Place of Presentation
      チェンマイ(タイ)
    • Related Report
      2007 Annual Research Report
  • [Book] 産業用酵素の応用技術と最新動向(井上國世監修)2009

    • Author(s)
      渡邉剛志他
    • Publisher
      シーエムシー出版
    • Related Report
      2008 Final Research Report
  • [Book] キチン・キトサン開発技術(平野茂樹監修, 第6章2 : 微生物のファミリー19キチナーゼ)2009

    • Author(s)
      渡邉剛志他
    • Publisher
      シーエムシー出版
    • Related Report
      2008 Final Research Report
  • [Book] キチン・キトサン開発技術(平野茂樹監修, 第7章4 : キチナーゼによる結晶性キチン分解)2009

    • Author(s)
      渡邉剛志他
    • Publisher
      シーエムシー出版
    • Related Report
      2008 Final Research Report
  • [Book] 酵素ハンドブック(八木達彦・福井俊郎・一島英治・鏡山博行・虎谷哲夫編)2008

    • Author(s)
      渡邉剛志他
    • Publisher
      朝倉書店
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.agr.niigata-u.ac.jp/profile/watanabe/index.html

    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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