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Metabolic engineering of ethanologenic bacteria based on transcriptome analysis

Research Project

Project/Area Number 19580090
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionTottori University

Principal Investigator

YANASE Hideshi  Tottori University, 工学研究科, 教授 (20158033)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Kenji  鳥取大学, 工学研究科, 准教授 (80283969)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsバイオマスエネルギー / 微生物遺伝学 / 代謝工学 / ゲノミクス / バイオエタノール / トランスクリプトーム / Zymobacter palmae / セルロース系バイオマス / ゲノムマップ / リグノセルロース
Research Abstract

The purpose of this study is the development of genetically engineered ethanologenic bacteria, Zymobacter and Zymomonas strains, capable of producing bioethanol from lignocellulosic biomass directly. We have been clarifying the important genes taking part in an efficient production of bioethanol in these bateria. First of all, the whole genome sequences of the strains were analyzed completely, and then the DNA microarray was constructed based on our genome information. By the DNA microarray analyses of recombinant strains during ethanol fermentation, we have found several important genes functioning in the ethanol production and in sugar transport.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (50 results)

All 2010 2009 2008 2007 2005

All Journal Article (16 results) (of which Peer Reviewed: 14 results) Presentation (21 results) Book (6 results) Patent(Industrial Property Rights) (7 results) (of which Overseas: 1 results)

  • [Journal Article] セルロース系バイオエタノール高速発酵細菌のメタボリックエンジニアリング2010

    • Author(s)
      簗瀬英司
    • Journal Title

      JARUS No.4

      Pages: 24-30

    • Related Report
      2009 Annual Research Report 2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Genomic Structure of Thermus Bacteriophage IN932009

    • Author(s)
      Matsushita, I., Yanase, H.
    • Journal Title

      J. Biochem. 146(6)

      Pages: 775-785

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Nobel Insertion Sequence Transposed to Thermophillic Bacteriophage IN932009

    • Author(s)
      Matsushita, I., Yanase, H.
    • Journal Title

      J. Biochem. 146(6)

      Pages: 797-803

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] スイートソルガムの搾汁に関する研究2009

    • Author(s)
      孫孝政、山名伸樹、簗瀬英司
    • Journal Title

      農業機械学会誌 71(4)

      Pages: 69-73

    • NAID

      10025393981

    • Related Report
      2009 Annual Research Report 2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel thermophilic lysozyme from b acteriophage IN932009

    • Author(s)
      Matsushita, I., Yanase, H.
    • Journal Title

      Biochem. Biophs. Res. Com. 377

      Pages: 89-92

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Genomic Structure of Thermus Bacteriophage・・IN932009

    • Author(s)
      Matsushita, I., Yanase, H.
    • Journal Title

      J. Biochem. 146(6)(●)

      Pages: 775-785

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Nobel Insertion Sequence Transposed to Thermophillic Bacteriophage・IN932009

    • Author(s)
      Matsushita, I., Yanase, H.
    • Journal Title

      J. Biochem 146(6)

      Pages: 797-803

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      簗瀬英司
    • Journal Title

      産業用酵素の応用技術と最新動向「多機能転移酵素による新規配糖体の合成」(監修:井上國世)(シーエムシー出版)

      Pages: 308-319

    • Related Report
      2009 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      渡辺隆司、簗瀬英司
    • Journal Title

      次世代バイオエタノール生産の技術革新と事業展開「担子菌・マイクロ波照射前処理と高速発酵細菌を用いる高効率バイオエタノール生産システム」(監修:鮫島正浩)(フロンティア出版)

      Pages: 287-294

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Decrease in hydrogen sulfide content during the final stage of beer fermentation due ti involvement of yeast and not carbon dioxide gas purging2008

    • Author(s)
      Oka, K., Matsumoto, N., Yanase, H.
    • Journal Title

      J. Biosci. Bioeng. 106

      Pages: 253-258

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decrease in hydrogen sulf ide content during the final stage of beer fermentation due ti involvement of yeast and not carbon dioxide gas purging2008

    • Author(s)
      K. Oka, N. Matsumoto, H. Yanase
    • Journal Title

      J. Biosci. Bioeng. 106

      Pages: 253-257

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel thermophilic lysozyme from bacter iophage φIN932008

    • Author(s)
      I. Natsushita, H. Yanase
    • Journal Title

      Biochem. Biophys. Res. Com. 377

      Pages: 89-92

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic engineering of Zymobacter palmae for production of ethanol from xylose2007

    • Author(s)
      Yanase, H., Okamoto, K.
    • Journal Title

      Appl. Environ. Microbiol 73

      Pages: 2592-2599

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stabilized production of highly concentrated bioethanol from f ermentation broths by Zymomonas mobil is by pervaporation using silicone ru bber-coated silicalite membrane2007

    • Author(s)
      Yanase, H.
    • Journal Title

      J. C hem. Technol. Biotechnol. 82

      Pages: 745-751

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic engineering of Zymobacter palmae for production of ethanol from xylose2007

    • Author(s)
      Hideshi Yanase
    • Journal Title

      Appl. Environ. Microbiol 73(8)

      Pages: 2592-2599

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stabilized production of highly concentrated bioethanol from fermentation broths by Zymomonas mobilis by pervaporation using silicone rubber coated silicalite membrane2007

    • Author(s)
      Hideshi Yanase
    • Journal Title

      J. Chem. Technol. Biotechnol. 82

      Pages: 745-751

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] リグノセルロース糖化並行発酵細菌の代謝工学的育種2010

    • Author(s)
      小島基、岡本賢治、簗瀬英司
    • Organizer
      日本バイオマス科学会議
    • Place of Presentation
      東京都文京区
    • Year and Date
      2010-01-20
    • Related Report
      2009 Final Research Report
  • [Presentation] リグノセルロース糖化並行発酵細菌の代謝工学的育種2010

    • Author(s)
      小島基、岡本賢治、簗瀬英司
    • Organizer
      日本バイオマス科学会議
    • Place of Presentation
      東京
    • Year and Date
      2010-01-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規エタノール発酵細菌・ザイモバクター染色体DNAへのキシロース発酵性遺伝子の組み込み2009

    • Author(s)
      簗瀬英司、岡崎悟志, 岡本賢治
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規エタノール発酵細菌・ザイモバクターが分泌生産するセルラーゼ遺伝子のクローニング2009

    • Author(s)
      奥田洋、山本泰久、岡本賢治、簗瀬英司
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規エタノール発酵細菌・ザイモバクターのセロオリゴ糖・糖化発酵性の改良2009

    • Author(s)
      小島基, 岡本賢治, 簗瀬英司
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cellulomonas gelidaが分泌生産するエンドグルカナーゼ遺伝子のクローニング2009

    • Author(s)
      松浦静、萬本珠母、岡本賢治、簗瀬英司
    • Organizer
      日本生物工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Bioethanol production process from woody biomass using fungal and microwave irradiation pretreatments and genetically engineered bactreria2009

    • Author(s)
      Watanabe, T., Yanase, H.
    • Organizer
      SIM Annual Meeting & Exhibition
    • Place of Presentation
      カナダ
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ゲノム情報に基づくリグノセルロース糖化並行発酵細菌の育種戦略2008

    • Author(s)
      簗瀬
    • Organizer
      日本生物工学会
    • Place of Presentation
      東北学院大
    • Year and Date
      2008-08-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 新規エタノール発酵細菌・ザイモバクターヘのC5・C6糖並行発酵性付与2008

    • Author(s)
      岡崎, 岡本, 築瀬
    • Organizer
      日本生物工学会
    • Place of Presentation
      東北学院大
    • Year and Date
      2008-08-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 新規エタノール発酵細菌・ザイモバクターヘのセロオリゴ糖・糖化発酵性付与2008

    • Author(s)
      小島, 岡本, 築瀬
    • Organizer
      日本生物工学会
    • Place of Presentation
      東北学院大
    • Year and Date
      2008-08-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] リグノセルロース糖化並行発酵細菌のメタボリツクエンジニアリング2008

    • Author(s)
      築瀬
    • Organizer
      日本農芸化学会中部支部
    • Place of Presentation
      三重大学
    • Year and Date
      2008-06-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 未利用木質系バイオマスからの高速度バイオエタノール製造技術2008

    • Author(s)
      簗瀬英司
    • Organizer
      GSCN
    • Place of Presentation
      学術総合センター
    • Year and Date
      2008-03-09
    • Related Report
      2007 Annual Research Report
  • [Presentation] Zymomonas mobilis由来fructosyltransferaseの転移反応機構に関わるアミノ酸残基の解析2007

    • Author(s)
      丹下耕太、簗瀬英司, 他
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島大学
    • Year and Date
      2007-09-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] Nicotinoprotein: Formaldehyde dismutaseのNAD(H)結合ドメイン解析2007

    • Author(s)
      村上博充、簗瀬英司, 他
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島大学
    • Year and Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] 新規エタノール発酵細菌・ザイモバクターへの発酵特性改良2007

    • Author(s)
      荻野彩、簗瀬英司, 他
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島大学
    • Year and Date
      2007-09-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] 新規エタノール発酵細菌・ザイモバクターへのヘミセルロース分解性付与2007

    • Author(s)
      小島基、簗瀬英司, 他
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島大学
    • Year and Date
      2007-09-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] エタノール高速度発酵性細菌・ザイモモナスのキシロース発酵特性の改良2007

    • Author(s)
      西村健太郎、簗瀬英司, 他
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島大学
    • Year and Date
      2007-09-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] Klebsiella pseumoniae由来シアン分解酵素遺伝子のクローニング2007

    • Author(s)
      井上寿樹、簗瀬英司, 他
    • Organizer
      日本生物工学会
    • Place of Presentation
      広島大学
    • Year and Date
      2007-09-25
    • Related Report
      2007 Annual Research Report
  • [Presentation] 2相式メタン発酵槽を用いる固形有機廃棄物処理2007

    • Author(s)
      藤森愛、簗瀬英司, 他
    • Organizer
      日本農芸化学会中四国支部大会
    • Place of Presentation
      山口大学
    • Year and Date
      2007-09-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] ゲノム情報に基づく木質バイオエタノール高速発酵細菌のメタボリックエンジニアリング2005

    • Author(s)
      簗瀬英司
    • Organizer
      生化学会中四国支部大会シンポジウム
    • Place of Presentation
      鳥取市
    • Year and Date
      2005-05-15
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Zymomonas mobilisにおけるセロオリゴ糖・糖化発酵代謝の改変2005

    • Author(s)
      奥田洋, 幡部賢, 岡本賢治, 簗瀬英司
    • Organizer
      生化学会中四国支部大会
    • Place of Presentation
      鳥取市
    • Year and Date
      2005-05-15
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Book] 産業用酵素の応用技術と最新動向「多機能転移酵素による新規配糖体の合成」2009

    • Author(s)
      簗瀬英司
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] 担子菌・マイクロ波照射前処理と高速発酵細菌を用いる高効率バイオエタノール生産システム2009

    • Author(s)
      渡辺隆司、簗瀬英司
    • Publisher
      フロンティア出版
    • Related Report
      2009 Final Research Report
  • [Book] ザイモモナス菌によるエタノール発酵2008

    • Author(s)
      簗瀬英司
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] バイオリフアイナリー技術の工業最前線2008

    • Author(s)
      築瀬
    • Publisher
      シーエムシー出版
    • Related Report
      2008 Annual Research Report
  • [Book] 木質系バイオマスからの高速度バイオエタノール製造技術2007

    • Author(s)
      簗瀬英司
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2009 Final Research Report
  • [Book] バイオ液体燃料2007

    • Author(s)
      簗瀬英司
    • Total Pages
      489
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] グルコース・マンノース・キシロース並行発酵細菌およびそのれを用いるバイオエタノールの製造方法2008

    • Inventor(s)
      簗瀬英司、岡本賢治
    • Industrial Property Rights Holder
      鳥取大学
    • Filing Date
      2008-03-10
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 木質系および草本系バイオマス糖化液からのバイオエタノール並行発酵菌2008

    • Inventor(s)
      簗瀬英司、岡本賢治
    • Industrial Property Rights Holder
      鳥取大学
    • Filing Date
      2008-12-24
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] グルコース・マンノース・キシロース並行発酵細菌およびそれを用いるバイオエタノールの製造方法2008

    • Inventor(s)
      簗瀬英司、岡本賢治
    • Industrial Property Rights Holder
      鳥取大学
    • Filing Date
      2008-05-16
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 木質系および草本系バイオマス糖化液からのバイオエタノール並行発酵菌2008

    • Inventor(s)
      簗瀬岡本
    • Industrial Property Rights Holder
      鳥取大学
    • Filing Date
      2008-12-24
    • Related Report
      2008 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] グルコース・マンノース・キシロース並行発酵細菌およびそれを用いるバイオエタノールの製造方法2008

    • Inventor(s)
      築瀬岡本
    • Industrial Property Rights Holder
      鳥取大学
    • Acquisition Date
      2008-05-16
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造技術2007

    • Inventor(s)
      簗瀬英司、岡本賢治
    • Industrial Property Rights Holder
      鳥取大学
    • Filing Date
      2007-10-05
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] グルコース・マンノース・キシロース並行発酵性菌およびそれを用いるバイオエタノールの製造技術2007

    • Inventor(s)
      簗瀬英司
    • Industrial Property Rights Holder
      鳥取大学
    • Filing Date
      2007-10-05
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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