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Salt-inducible chiral-nylon polymer from Bacillus megaterium : Synthesis and biochemical applications including an increase in halo-tolerance

Research Project

Project/Area Number 19580092
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionKochi University

Principal Investigator

ASHIUCHI Makoto  Kochi University, 教育研究部自然科学系, 准教授 (20271091)

Co-Investigator(Kenkyū-buntansha) 永田 信治  高知大学, 農学部, 教授 (60180494)
Co-Investigator(Renkei-kenkyūsha) NAGATA Shinji  高知大学, 教育研究部自然科学系, 教授 (60180494)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords微生物遺伝・育種 / 環境材料 / 生体材料 / 生体機能利用 / バイオテクノロジー / 環境適応
Research Abstract

We identified the industrial strain of Bacillus megaterium as a novel producer of L-type stereo-regular poly-γ-glutamate (PGA). Advanced analysis about PGA production of B. megaterium and cloning and sequencing of the genetic cluster responsible for the polymer synthesis and degradation, to examine stereo-regular PGA metabolism, were performed in the study. Additionally, in vivo and in vitro applicability of stereo-regular PGA as extremolyte-like materials was investigated as well.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (49 results)

All 2010 2009 2008 2007 Other

All Journal Article (14 results) (of which Peer Reviewed: 8 results) Presentation (21 results) Book (7 results) Remarks (2 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 1 results)

  • [Journal Article] Identification and biochemical characterization of membranous shortchain polyglutamate from Bacillus subtilis2010

    • Author(s)
      M.Ashiuchi, et al.(5人中5番目)
    • Journal Title

      Chem. Biodiv (印刷中)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Extremolyte-like applicability of an archaeal exopolymer, poly-γ-Lglutamate2010

    • Author(s)
      M.Ashiuchi, et al.(3人中3番目)
    • Journal Title

      Environ. Technol (印刷中)

    • NAID

      40019308385

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] ポリ-γ-グルタミン酸の新用途展開2010

    • Author(s)
      芦内誠
    • Journal Title

      BIO INDUSTRY 27巻

      Pages: 49-55

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] ポリ-γ-グルタミン酸の新用途展開2010

    • Author(s)
      芦内誠
    • Journal Title

      BIO INDUSTRY 27

      Pages: 49-55

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Extremolyte-like applicability of an archaeal exopolymer, poly-γ-L-glutamate2010

    • Author(s)
      D.Yamasaki, et al.
    • Journal Title

      Environ.Technol. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and biochemical characterization of membranous short-chain polyglutamate from Bacillus subtilis2010

    • Author(s)
      T.Kamei, et al.
    • Journal Title

      Chem.Biodiv. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] D-グルタミン酸とポリ-γ-グルタミン酸合成システム2008

    • Author(s)
      芦内誠
    • Journal Title

      生化学会誌 80巻

      Pages: 316-323

    • NAID

      10021191585

    • Related Report
      2009 Final Research Report
  • [Journal Article] バイオキラルナイロンの生産に関する微生物化学的基礎研究2008

    • Author(s)
      芦内誠
    • Journal Title

      生物工学会誌 86巻

      Pages: 73-79

    • NAID

      110006623902

    • Related Report
      2009 Final Research Report
  • [Journal Article] D-グルタミン酸とポリ-γ-グルタミン酸合成システム2008

    • Author(s)
      芦内 誠
    • Journal Title

      生化学会誌 80

      Pages: 316-323

    • NAID

      10021191585

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Genetic design of conditional D-glutamate auxotrophy for Bacillus subtili Use of a vector-bome poly-γ-glutamate synthetic system2008

    • Author(s)
      M.Ashiuchi, et. al.
    • Journal Title

      Biochem.Biophys.Res.Commun 362

      Pages: 646-650

    • Related Report
      2007 Annual Research Report
  • [Journal Article] バイオキラルナイロンの生産に関する微生物化学的基礎研究2008

    • Author(s)
      芦内 誠
    • Journal Title

      生物工学会誌 86

      Pages: 73-79

    • NAID

      110006623902

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Genetic design of conditional Dglutamate auxotrophy for Bacillus subtilis: Use of a vector-borne poly-γ-glutamate synthetic system2007

    • Author(s)
      M.Ashiuchi, et al.(6人中1番目)
    • Journal Title

      Biochem. Biophys. Res. Commun 362巻

      Pages: 646-650

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Salt-inducible bionylon polymer from Bacillus megaterium2007

    • Author(s)
      M.Ashiuchi, et al.(3人中3番目)
    • Journal Title

      Appl. Environ. Microbiol 73巻

      Pages: 2378-2379

    • NAID

      120001083657

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Salt-inducible bionylon polymer from Bacillus megaterium2007

    • Author(s)
      K.Shimizu, H.Nakamura, and M.Ashiuchi
    • Journal Title

      Appl.Environ.Microbiol 73

      Pages: 2378-2379

    • NAID

      120001083657

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 固体13CNMRによるラセミ化率が異なるポリ-γ-グルタミン酸の研究2010

    • Author(s)
      平沖敏文, ほか
    • Organizer
      日本高分子学会年次大会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] ポリ-γ-グルタミン酸を基盤とする制菌プラスチック新素材2010

    • Author(s)
      吉岡恵, ほか
    • Organizer
      日本農芸化学会年次大会
    • Place of Presentation
      東京都・東京大学
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Identification of a Membranous short-chain poly-γ- glutamate from Bacillus subtilis and its biochemical characteristics2009

    • Author(s)
      M.Ashiuchi, et al
    • Organizer
      日本生化学会年次大会
    • Place of Presentation
      神戸市・神戸ポートアイランド
    • Related Report
      2009 Final Research Report
  • [Presentation] 立体規則性ポリ-γ-グルタミン酸のバイオプラスチック化2009

    • Author(s)
      芦内誠, ほか
    • Organizer
      第58回高分子討論会
    • Place of Presentation
      熊本県・熊本大学
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Moonlighting function of the pgsE-gene product, a novel member of the membranous enzyme complex responsible for the synthesis of D-glutamate-containing poly-γ- glutamate2009

    • Author(s)
      M.Ashiuchi, et al
    • Organizer
      The first International Conference of D-Amino Acid Research (IDAR)
    • Place of Presentation
      路市、夢舞台国際会議場
    • Related Report
      2009 Final Research Report
  • [Presentation] 巨大菌Bacillus megateriumにおけるポリ-γ-グルタミン酸の生産特性2009

    • Author(s)
      吉岡恵, ほか
    • Organizer
      日本農芸化学会年次大会
    • Place of Presentation
      福岡市・マリンメッセ福岡
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification of a Membranous short-chain poly-γ-glutamate from Bacillus subtilis and its biochemical characteristics2009

    • Author(s)
      M.Ashiuchi, et al.
    • Organizer
      日本生化学会年次大会
    • Place of Presentation
      神戸市・神戸ポートアイランド
    • Related Report
      2009 Annual Research Report
  • [Presentation] 巨大菌Bacillus megateriumにおけるポリ-γ-グルタミン酸の生産特性2009

    • Author(s)
      吉岡 恵, 蓑内 裕。芦内 誠
    • Organizer
      日本農芸化学会(年次大会)
    • Place of Presentation
      福岡市・マリンメッセ福岡
    • Related Report
      2008 Annual Research Report
  • [Presentation] Bacillus subtilis ywtC (edmS) is an unexpected plasmid-maintenance gene in the poly-γ-glutamate biosynthetic operon2008

    • Author(s)
      M.Ashiuchi, et al
    • Organizer
      BMB2008
    • Place of Presentation
      神戸市・神戸ポートアイランド
    • Related Report
      2009 Final Research Report
  • [Presentation] Biochemical synthesis of stereo-regular poly-γ-Glutamate2008

    • Author(s)
      M.Ashiuchi
    • Organizer
      The 4th Japan-Netherlands Joint Seminar
    • Place of Presentation
      福岡市・九州大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 立体規則性ポリ-γ-グルタミン酸の効率的な化学改質法2008

    • Author(s)
      福島賢三, ほか
    • Organizer
      日本生物工学会年次大会
    • Place of Presentation
      仙台市・東北学院大学
    • Related Report
      2009 Final Research Report
  • [Presentation] ポリ-γ-グルタミン酸:生合成機構から応用まで2008

    • Author(s)
      芦内誠
    • Organizer
      日本農芸化学会年次大会
    • Place of Presentation
      名古屋市・名城大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 立体規則性ポリ-γ-グルタミンの効率的な化学改質法2008

    • Author(s)
      福島賢三, 芦内誠
    • Organizer
      日本生物工学会(年次大会)
    • Place of Presentation
      仙台市・東北学院大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] Biochemical Synthesis of Stereo-Regular Poly-γ-Glutamate2008

    • Author(s)
      Makoto Ashiuchi
    • Organizer
      The 4th Japan-Netherlands Joint Seminar
    • Place of Presentation
      福岡市・九州大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] Bacillus subtills ywtC (edmS) is an unexpected plasmid-maintenancegene in the poly-γ-glutamate biosynthetic operon2008

    • Author(s)
      Makoto Ashiuchi, Daisuke Yamashiro
    • Organizer
      BMB2008
    • Place of Presentation
      神戸市・神戸ポートアイランド
    • Related Report
      2008 Annual Research Report
  • [Presentation] Salt-inducible bionylon polymer: Microbial synthesis and biochemical application2007

    • Author(s)
      M.Ashiuchi, et al
    • Organizer
      The 10th Pacific Polymer Conference (PPC10)
    • Place of Presentation
      神戸市・神戸国際会議場
    • Related Report
      2009 Final Research Report
  • [Presentation] Study on a bionylon polymer, poly-γ-glutamate2007

    • Author(s)
      M.Ashiuchi
    • Organizer
      KSBB International Symposium
    • Place of Presentation
      韓国大邱市
    • Related Report
      2009 Final Research Report
  • [Presentation] バイオキラルナイロンの生産に関する微生物化学的基礎研究2007

    • Author(s)
      芦内誠
    • Organizer
      日本生物工学会年次大会
    • Place of Presentation
      広島県・広島大学
    • Related Report
      2009 Final Research Report
  • [Presentation] バイオキラルナイロンの生産に関する微生物化学的基礎研究2007

    • Author(s)
      芦内 誠
    • Organizer
      日本生物工学会(年次大会)
    • Place of Presentation
      広島県
    • Related Report
      2007 Annual Research Report
  • [Presentation] Study on a bionylon polymer, poly-γ-glutamate2007

    • Author(s)
      M.Ashiuchi
    • Organizer
      韓国KSBB秋季大会(招待講演)
    • Place of Presentation
      韓国大邸市
    • Related Report
      2007 Annual Research Report
  • [Presentation] Salt-inducible bionylon polymer:Microbial synthesis and biochemial application2007

    • Author(s)
      M.Ashiuchi, et. al.
    • Organizer
      The 10th Pacific Polymer Conference(PPC10)(国際学会)
    • Place of Presentation
      兵庫県(神戸市)
    • Related Report
      2007 Annual Research Report
  • [Book] Occurrence and biosynthetic mechanism of poly-γ-glutamic acid. In: Amino-Acid Homopolymers Occurring in Nature2010

    • Author(s)
      M.Ashiuchi
    • Publisher
      Springer-Verlag (Heidelberg)(印刷中)
    • Related Report
      2009 Final Research Report
  • [Book] Occurrence and biosynthetic mechanism of poly-γ-glutamic acid. In : Microbiol.Monogr. (Amino-Acid Homonolymers Occurring in Nature ; Y. Hamano, ed.)2010

    • Author(s)
      M.Ashiuchi
    • Publisher
      Springer-Verlag(Heidelberg)(印刷中)
    • Related Report
      2009 Annual Research Report
  • [Book] 産業用酵素の応用技術と最新動向2009

    • Author(s)
      芦内誠
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] 産業用酵素の応用技術と最新動向(分担執筆)2009

    • Author(s)
      芦内 誠
    • Total Pages
      13
    • Publisher
      シーエムシー出版
    • Related Report
      2008 Annual Research Report
  • [Book] ホワイトバイオテクノロジー;エネルギー・材料の最前線2008

    • Author(s)
      芦内誠
    • Publisher
      シーエムシー出版
    • Related Report
      2009 Final Research Report
  • [Book] ホワイトバイオテクノロジー ; エネルギー・材料の最前線(分担執筆)2008

    • Author(s)
      芦内 誠
    • Total Pages
      12
    • Publisher
      シーエムシー出版
    • Related Report
      2008 Annual Research Report
  • [Book] Micro-purification and structural assays of poly-γ-glutamate, a D-amino acid-containing biopolymer, In: D-Amino Acids: A New Frontier in Amino Acid and Protein Research-Practical Methods and Protocols2007

    • Author(s)
      M.Ashiuchi, H.Misono
    • Publisher
      Nova Science Publishers
    • Related Report
      2009 Final Research Report
  • [Remarks] 国際バイオフォーラム~バイオアカデミックフォーラム~(主催:エグジビションジャパン)、参加・技術説明、2009.7.1~3、東京都・東京ビックサイト

    • Related Report
      2009 Final Research Report
  • [Remarks] イノベーションブリッジ四国地区四大学研究発表会(主催:jst)、参加・技術説明、2007.12.14、東京・秋葉原

    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] pgaイオンコンプレックス2009

    • Inventor(s)
      芦内誠, 福島賢三
    • Industrial Property Rights Holder
      高知大学
    • Industrial Property Number
      2009-072596
    • Filing Date
      2009-03-24
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] ポリ-γ-グルタミン酸架橋体の製造方法およびポリ-γ-グルタミン酸架橋体からなる成形体2009

    • Inventor(s)
      芦内誠, 福島賢三
    • Industrial Property Rights Holder
      高知大学
    • Patent Publication Number
      2009-209362
    • Filing Date
      2009-02-05
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] PGAイオンコンプレックス2009

    • Inventor(s)
      芦内 誠福島賢三
    • Industrial Property Rights Holder
      高知大学
    • Industrial Property Number
      2009-072596
    • Filing Date
      2009-03-24
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] Process for producing poly-γ- glutamic acid having high optical purity2008

    • Inventor(s)
      M.Ashiuchi, K.Shimizu
    • Industrial Property Rights Holder
      Kochi University
    • Filing Date
      2008-07-15
    • Related Report
      2009 Final Research Report
  • [Patent(Industrial Property Rights)] 光学純度の高いポリ-γ-グルタミン酸の製造方法2007

    • Inventor(s)
      芦内 誠, 清水 一希
    • Industrial Property Rights Holder
      高知大学
    • Filing Date
      2007-01-31
    • Related Report
      2007 Annual Research Report
    • Overseas

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Published: 2007-04-01   Modified: 2016-04-21  

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