Global Regulation of expression of the Bacillus subtilis aprE gene encoding the extracellular alkaline protease

• Project/Area Number: 19580094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokai University
• Principal Investigator: 田中 暉夫 Tokai University, 海洋研究所, 教授 (10236606)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 枯草菌 / アルカリプロテアーゼ / aprE遺伝子 / scoC遺伝子 / glnA遺伝子 / tnrA遺伝子 / degR遺伝子 / 枯草菌アルカリプロテアーゼ / sigD遺伝子 / 転写制御因子GlnRとTnrA

Research Abstract

土壌や根圏に生息する微生物である枯草菌は細胞外に多量の蛋白質分解酵素であるプロテアーゼを分泌する。プロテアーゼの役割は、細胞の周辺にたまたま存在する動植物の破片やその細胞内容物に含まれるタンパク質をアミノ酸またはペプチドにまで分解し、細胞に取り込まれる形にするものと考えられる。しかし、枯草菌は必要な時にだけ、すなわち、栄養が不足した時にだけプロテアーゼを分泌する。本研究は、このような制御のメカニズム解明を試みた。その結果、グルタミン合成酵素が細胞内の栄養(窒素源)状態を感知し、その制御下にある種々の転写制御因子をコントロールしてプロテアーゼの産生を制御しているという結論を得た。

Research Products

• [Journal Article] Regulation of Bacillus subtilis aprE expression by glnA through inhibition of scoC and sD-dependent degR expression (2009)
  • Author(s): Abe, S., Yasumura, A., and Tanaka, T.
  • Journal Title: J. Bacteriol 191 Pages: 3050-3058
  • Peer Reviewed
• [Journal Article] Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD) (2009)
  • Author(s): Ogura, M. and Tanaka, T.
  • Journal Title: J. Bacteriol. 191 Pages: 949-958
  • Peer Reviewed
• [Journal Article] Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD) (2009)
  • Author(s): Ogura, M., Tanaka, T.
  • Journal Title: Journal of Bacteriology 191 Pages: 949-958
  • Peer Reviewed
• [Journal Article] Regulation of Bacillus subtilis aprE expression by glnA through inhibition of scoC and σ^D-dependent degR expression. (2009)
  • Author(s): Abe, S., Yasumura, A., Tanaka, T.
  • Journal Title: Journal of Bacteriology 191(In press)
  • Peer Reviewed
• [Journal Article] Involvement of nitrogen regulation in Bacillus subtilis degU expression (2008)
  • Author(s): Yasumura, A., Abe, S. and Tanaka, T.
  • Journal Title: J. Bacteriol 190 Pages: 5162-5171
  • Peer Reviewed
• [Journal Article] Identification of the sequences recognized by the Bacillus subtilis response regulator YrkP (2008)
  • Author(s): Ogura, M., Ohsawa, T. and Tanaka, T.
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 186-196
  • Peer Reviewed
• [Journal Article] Involvement of nitrogen regulation in Bacillus subtilis degU expression (2008)
  • Author(s): Yasumura, A., Abe, S, Tanaka, T.
  • Journal Title: Journal of Bacteriology 190 Pages: 5162-5171
  • Peer Reviewed
• [Journal Article] The NatK-NatR two-component system regulates expression of natAB operon encoding an ABC transporter for sodium ion extrusion (2007)
  • Author(s): Ogura, M., Tsukahara, K., Hayashi, K. and Tanaka, T.
  • Journal Title: Microbiology 153 Pages: 667-675
  • Peer Reviewed
• [Journal Article] Identification of the sequences recognized by the Bacillus subtilis response regulator YrkP (2007)
  • Author(s): Ogura. M., Ohsawa, T. and Tanaka, T.
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 186-196
  • Peer Reviewed
• [Presentation] 枯草菌グルタミン合成酵素遺伝子glnAによるdegU転写制御へのdegRの関与 (2009)
  • Author(s): 安村彩子、阿部定信、田中暉夫
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
• [Presentation] 枯草菌の後期competenceオペロンcomEのYutBとComNによる制御 (2009)
  • Author(s): 小倉光雄、田中暉夫
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
• [Presentation] 枯草菌グルタミン合成酵素遺伝子glnAによるdegU転写制御へのdegRの関与 (2009)
  • Author(s): 安村彩子, 阿部定信, 田中暉夫
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡マリンメッセ
• [Presentation] 枯草菌グルタミン合成酵素遺伝子によるdegUの転写制御 (2008)
  • Author(s): 安村彩子、阿部定信、田中暉夫
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
• [Presentation] 枯草菌グルタミン合成酵素遺伝子によるdegUの転写制御 (2008)
  • Author(s): 安村 彩子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋名城大学
• [Presentation] The NatK-NatR two-component system regulates expression of the natAB operon encoding an ABC transporter for sodium ion extrusion (2007)
  • Author(s): Ogura, M., Tsukahara, K., Hayashi, K., and Tanaka, T.
  • Organizer: 4th Conference on Functional Genomics of Gram-positive Microorganisms
  • Place of Presentation: Pisa, Italy.
• [Book] Regulation of the Bacillus subtilis alkaline protease gene, aprE, by many regulatory factors.Global regulatory networks in Bacillus subtilis.(In Y. Fujita (ed.)) (2007)
  • Author(s): Tanaka, T.
  • Publisher: Transworld Research Network, Keraia, India
• [Book] Global Regulatory Networks in Bacillus subtilis (2007)
  • Author(s): Teruo Tanaka
  • Publisher: Transworld Research Network