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Pure isolation of nitrite-oxidizing bacteria from several environments and development of new phylogenetic marker for them.

Research Project

Project/Area Number 19580096
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionNihon University

Principal Investigator

TAKAHASHI Reiji  Nihon University, 生物資源科学部, 准教授 (70197193)

Co-Investigator(Kenkyū-buntansha) TOKUYAMA Tatsuaki  日本大学, 生物資源科学部, 教授 (90059684)
NAKAGAWA Tatsunori  日本大学, 生物資源科学部, 講師 (40434104)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords環境微生物学 / 亜硝酸酸化菌 / 分子系統分類 / 亜硝酸酸化還元酵素遺伝子 / nxrB / 新規分離 / 分布解析 / 微生物群集構造解析 / DGGE / Nitrobacter / norB
Research Abstract

Nitrite oxidizing bacteria (NOB) that inhabit the whole environment extensively are bacterial species that are in charge of the latter part of nitrification. But little strains of them are isolated as pure culture. This study were carried out for exploration and its application of useful of genetic resources and showed following knowledge. First, as many NOB strains were newly isolated from a variety of environments, we got sufficient strains for studying microbial properties of them. Second, we explored new markers for phylogenetic classification for NOB. Third, we established a new phylogenetic classification with new markers, and we have applied it to "non-culture" analysis of NOB in natural environments.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2010 2009 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (9 results) Book (3 results) Remarks (3 results)

  • [Journal Article] Succession and community composition of ammonia-oxidizing archaea and bacteria in bulk soil of a Japanese paddy field.2010

    • Author(s)
      Fujii, C., T. Nakagawa, Y. Onodera, N. Matsutani, K. Sasada, R. Takahashi, T. Tokuyama
    • Journal Title

      Soil Sci. Plant Nutr (In Press)

    • NAID

      110008144314

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal change in vertical distribution of ammonia-oxidizing archaea and bacteria and their nitrification in temperate forest soil.2010

    • Author(s)
      Onodera, Y., T. Nakagawa, R. Takahashi, T. Tokuyama
    • Journal Title

      Microbes Environ 25

      Pages: 28-35

    • NAID

      10025921560

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal change in vertical distribution of ammonia-oxidizing archaea and bacteria and their nitrification in temperate forest soil2010

    • Author(s)
      Yuki Onodera, Tatsunori Nakagawa, Reiji Takahashi, Tatsuaki Tokuyama
    • Journal Title

      Microbes and Environments 25

      Pages: 28-35

    • NAID

      10025921560

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of ammonia monooxygenase and archaeal 16S rRNA gene fragments in nitrifying acid-sulfate soil microcosms.2009

    • Author(s)
      Nakaya, A., Y. Onodera, T. Nakagawa, K. Satoh, R. Takahashi, S. Sasaki, T. Tokuyama
    • Journal Title

      Microbes Environ 24

      Pages: 168-174

    • NAID

      10025920328

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal changes in abundance of ammonia-oxidizing archaea and ammonia-oxidizing bacteria and their nitrification in sand of an eelgrass zone.2009

    • Author(s)
      Ando, Y., T. Nakagawa, R. Takahashi, K. Yoshihara, T. Tokuyama
    • Journal Title

      Microbes Environ 1

      Pages: 21-27

    • NAID

      110007131155

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of ammonia monooxygenase and archaeal 16S rRNA gene fragments in nitrifying acid-sulfate soil microcosms2009

    • Author(s)
      Asami Nakaya, Yuki Onodera, Tatsunori Nakagawa, Kazuo Satoh, Reiji Takahashi, Satohiko Sasaki, Tatsuaki Tokuyama
    • Journal Title

      Microbes and Environments 24

      Pages: 168-174

    • NAID

      10025920328

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal changes in abundance of ammonia-oxidizing archaea and ammonia-oxidizing bacteria and their nitrification in sand of an eelgrass zone.2009

    • Author(s)
      Tatsunori Nakaga-wa, Reiji Takahashi, Kiyoshi Yoshi-hara, Tatsuaki Tokuyama
    • Journal Title

      Microbes and Environments 24

      Pages: 21-27

    • NAID

      110007131155

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of newly isolated ammonia-oxidizing bacteria from acid sulfate soil and the rhizoplane of leucaena grown in that soil.2007

    • Author(s)
      Satoh, K., Itoh, C., Kang, D.-J., Sumida, H., Takahashi, R., Isobe, K., Sasaki, S., Tokuyama T
    • Journal Title

      Soil Sci. Plant Nutr 53

      Pages: 23-31

    • NAID

      110006532361

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 根面に棲息する硝化細菌2007

    • Author(s)
      佐藤一朗、高橋令二、徳山龍明
    • Journal Title

      土と微生物 61

      Pages: 49-53

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of cold-adapted ammonia-oxidizing microorganisms in the deep-ocean of the northeastern Japan Sea2007

    • Author(s)
      Tatsunori Nakagawa, Koji Mori, Chiaki Kato, Reiji Takahashi, and Tatsuaki Tokuyama
    • Journal Title

      Microbes and Environments 22

      Pages: 365-372

    • NAID

      110006473363

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] PCR-DGGE分析による水族館ろ過槽ろ材における亜硝酸酸化菌Nitrospira属のモニタリング2009

    • Author(s)
      羽飼亮太, 中川達功, 植田育男, 高橋令二, 徳山龍明
    • Organizer
      第25回日本微生物生態学会大会
    • Place of Presentation
      広島大学
    • Year and Date
      2009-11-22
    • Related Report
      2009 Final Research Report
  • [Presentation] 酸性硫酸塩土壌から分離されたアンモニア酸化菌のウレアーゼの発現解析2009

    • Author(s)
      日比野倫士, 高橋令二, 中川達功, 佐藤一朗, 徳山龍明
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Final Research Report
  • [Presentation] 亜硝酸酸化菌Nitrospira属の16S rRNA遺伝子に特異的なPCRプライマーの開発2009

    • Author(s)
      羽飼亮太, 山根さつき, 藤井千尋, 安藤善史, 中川達功, 植田育男, 笹田勝寛, 高橋令二, 吉原喜好, 徳山龍明
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Final Research Report
  • [Presentation] 各種環境から分離されたNitrobacter属菌の特性とnxrB遺伝子による分類の検討2009

    • Author(s)
      石森英樹, 高橋令二, 中村渉, 山田静恵, 中川達功, 徳山龍明
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都大学
    • Year and Date
      2009-09-15
    • Related Report
      2009 Final Research Report
  • [Presentation] 各種環境から分離された Nitrobacter 属菌の特性とnxrB遺伝子による分類の検討2009

    • Author(s)
      石森英樹, 高橋令二, 中村渉, 山田静恵, 中川達功, 徳山龍明
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都・京都大学
    • Year and Date
      2009-09-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] 各種環境から分離されたNitrobacter属菌の特性とその分類2008

    • Author(s)
      石森英樹, 高橋令二, 佐藤有佑, 石山祥子, 中川達功, 徳山龍明
    • Organizer
      2008年度日本土壌肥料学会関東支部大会
    • Place of Presentation
      新潟大学
    • Year and Date
      2008-11-29
    • Related Report
      2009 Final Research Report
  • [Presentation] タイ王国酸性硫酸塩土壌から分離されたアンモニア酸化菌のウレアーゼの発現解析2008

    • Author(s)
      日比野倫士, 高橋令二, 中川達功, 佐藤一朗, 徳山龍明
    • Organizer
      2008年度日本土壌肥料学会関東支部大会
    • Place of Presentation
      新潟大学
    • Year and Date
      2008-11-29
    • Related Report
      2009 Final Research Report
  • [Presentation] 各種環境から分離されたNitrobacter属菌の特性とその分類2008

    • Author(s)
      石森英樹, 高橋令二, 佐藤有佑, 石山祥子, 中川達功, 徳山龍明
    • Organizer
      2008年度日本土壌肥料学会関東支部大会
    • Place of Presentation
      新潟・新潟大学
    • Year and Date
      2008-11-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 各種環境より分離した亜硝酸酸化菌の特性と新規分類指標の検討2007

    • Author(s)
      高橋令二, 中川達功, 徳山龍明
    • Organizer
      日本土壌肥料学会2007年度東京大会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2007-08-24
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Book] Overview of microorganisms isolated from acid sulfate soils2008

    • Author(s)
      Tokuyama, T
    • Publisher
      Shoukadoh
    • Related Report
      2009 Final Research Report
  • [Book] Analysis of microbial diversity, isolation of ammonia-oxidizing and sulfur-oxidizing bacteria in acid sulfate soil2008

    • Author(s)
      Takahashi, R., K. Satoh, T. Tokuyama
    • Publisher
      Shoukadoh
    • Related Report
      2009 Final Research Report
  • [Book] "Analysis of microbial diversity, isolation of ammonia-oxidizing and sulfur-oxidizing bacteria in acid sulfate soil" in Sasaki S(ed.), "Development of New Bioremediation Systems of Acid Sulfate Soil for Agriculture and Forestry"2008

    • Author(s)
      Reiji Takahashi, Kazuo Satoh, and Tatsuaki Tokuyama
    • Publisher
      Shoukadoh(Kyoto, Japan)
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://hp.brs.nihon-u.ac.jp/~biken/index/index.htm

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://hp.brs.nihon-u.ac.jp/~biken/index/index.htm

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://hp.brs.nihon-u.ac.jp/~biken/index/index.htm

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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