Analyses on periplasmic enzymes that covalently bind lipoproteins to peptidoglycan in Escherichia coli

• Project/Area Number: 19580099
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Rikkyo University
• Principal Investigator: MATSUYAMA Shinichi Rikkyo University, 理学部, 教授 (50183108)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 大腸菌 / 外膜 / リポタンパク質 / トランスペプチダーゼ / 細胞表層 / 内膜 / 生合成 / 局在化 / 化学療法剤 / リポ蛋白質

Research Abstract

YbiS and its paralogs, ErfK and YcfS, that are periplasmic proteins of Escherichia coli were biochemically and genetically identified as transpeptidases (TPase) to bind covalently lipoproteins to peptidoglycan. The result that YbiS and YcfS function acid-neutral and alkali conditions, respectively, strongly suggest that these TPase cooperatively work to adapt to the environmental change.

Research Products

• [Journal Article] Membrane Topology and Functional Importance of the Periplasmic Region of ABC Transporter LolCDE. (2009)
  • Author(s): Yasuda M., Iguchi-Yokoyama A., Matsuyama S., Tokuda H., Narita S.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 2310-2316
  • NAID: 10027547063
  • Peer Reviewed
• [Journal Article] Membrane Topology and Functional Importance of the Periplasmic Region of ABC Transporter LolCDE. (2009)
  • Author(s): Yasuda M., Matsuyama S., 他
  • Journal Title: Biosci.Biotechnol.Biochem 73 Pages: 2310-2316
  • NAID: 10027547063
  • Peer Reviewed
• [Journal Article] Structure, function and transport of lipoproteins in Escherichia coli. In The Periplasm, M. Ehrmann (ed) (2007)
  • Author(s): Tokuda, H., Matsuyama, S., Tanaka-Masuda, K.
  • Journal Title: ASM press Pages: 67-79
  • Peer Reviewed
• [Journal Article] A new screening method to identify inhibitors of Lol (localization of lipoproteins) system (2007)
  • Author(s): Ito, H., Ura, A., Oyamada, Y., Yoshida, H., Yamagishi, J., Narita, S., Matsuyama, S., Tokuda, H.
  • Journal Title: a novel antibacterial target. Microbiol. Immunol 51 Pages: 263-270
  • Peer Reviewed
• [Journal Article] A new screening method to identify inhibitors of Lol(localization of lipoproteins)system,a novel antibacterial target (2007)
  • Author(s): Ito, H
  • Journal Title: Microbiol.Immunol 51 Pages: 263-270
  • Peer Reviewed
• [Presentation] 大腸菌Lpp-ペプチドグリカントランスペプチダーゼ3遺伝子のpH変化に応答した発現 (2009)
  • Author(s): 福本玲
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-27
• [Presentation] 大腸菌主要外膜リポタンパク質lppをペプチドグリカンに共有結合させるトランスペプチダーゼ (2007)
  • Author(s): 田中大揮
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-12-14
• [Presentation] 大腸菌のlpp-ペプチドグリカントランスペプチダーゼの生化学的解析 (2007)
  • Author(s): 宮隆允
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-12-14
• [Presentation] 大腸菌主要外膜リポタンパク質Lppをペプチドグリカンに共有結合させるトランスペプチダーゼ (2007)
  • Author(s): 田中大揮
  • Organizer: 第4回21世紀大腸菌研究会
  • Place of Presentation: 静岡
  • Year and Date: 2007-07-17
• [Book] Structure, function and transport of lipoproteins in Escherichia coli (2007)
  • Author(s): Tokuda, H.
  • Publisher: ASM press
• [Remarks]
  • URL: http://www.rikkyo.ne.jp/univ/smatsuya/
• [Remarks] 立教大学
  • URL: http://www.rikkyo.ne.jp/grp/cri/ken/vin/konagai_k.html
• [Remarks] 立命館大学
  • URL: http://research-db.ritsumei.ac.jp/Profiles/34/0003339/profile.html