Energy charge in C4 photosynthesis through cyclic electron transport

• Project/Area Number: 19580105
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: TSUYOSHI Endo Kyoto University, 生命科学研究科, 講師 (90201962)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: C4光合成 / 循環的電子伝達系 / NAD(P)H デヒドロゲナーゼ / ATP 生成 / 環境 / 植物 / ストレス / 光合成 / C4植物

Research Abstract

葉緑体NDH複合体の未知サブユニットを同定する目的で、Arabidopsis共発現解析データベース等を利用して新規NDH遺伝子の選抜をした。その結果、5個の遺伝子の同定と解析をライバルに先駆けて、または同時に発表することができた。
C_4種、C_3種および intermediate のフラベリアを材料とし、4個のNDH サブユニットに対する抗体を用いてイムノブロット解析を行った。また、C_4フラベリア(F. bidenti)から、シロイヌナズナで同定された核ゲノムコードのNDHサブユニット遺伝子ndhNと本研究にて単離したndf4, ndf6のホモログをクローニングした。単離した遺伝子配列を利用してその遺伝子の発現抑制を引き起こすためのRNAiベクターを作成し、形質転換を行った。

Research Products

• [Journal Article] Distinct functions for the two psbP-like proteins PPL1 and PPL2 in the chloroplast thylakoid lumen of Arabidopsis (2009)
  • Author(s): Ishihara, S., Takabayashi, A., Ido, K., Endo, T., Ifuku, K. and Sato, F.
  • Journal Title: Plant Physiol 145 Pages: 668-679
• [Journal Article] Three novel subunits of Arabidopsis chloroplastic NAD(P)H dehydrogenase identified by bioinformatic and reverse genetic approaches (2009)
  • Author(s): Takabayashi, A., Ishikawa, N., Obayashi, T., Ishida, S., Obokata, J., Endo, T. and Sato, F.
  • Journal Title: Plant J 57 Pages: 207-219
• [Journal Article] A novel nuclear-encoded protein, NDH-Dependent cyclic electron Flow 5, is essential for the accumulation of the chloroplastic NAD(P)H dehydrogenase complexes (2009)
  • Author(s): Ishida, S., Takabayashi, A., Ishikawa, N., Hano, Y., Endo, T. and Sato, F.
  • Journal Title: Plant Cell Physiol 50 Pages: 383-393
  • NAID: 10027337854
• [Journal Article] A novel nuclear-encodea protein, NUH-Uependent cyclic electron Flow 5, is essential for the accumulation of the chloroplastic NAD(P)H dehydrogenase complexes. (2009)
  • Author(s): Ishida, S., Takabayashi, A., Ishikawa, N., Hano, Y., Endo, T., Sato, F.
  • Journal Title: Plant Cell Physiol. 50 Pages: 383-393
  • Peer Reviewed
• [Journal Article] Three novel subunits of Arabidopsis chloroplastic NAD(P)H dehydrogenase identified by bioinformatic and reverse genetic approaches. (2009)
  • Author(s): Takabayashi, A., Ishikawa, N., Obayashi, T., Ishida, S., Obokata, J., Endo, T., Sato, F.
  • Journal Title: Plant J. 57 Pages: 207-219
  • Peer Reviewed
• [Journal Article] Altered photosynthetic electron channeling into cyclic electronflow and nitrite assimilation in a mutant of ferredoxin : NADP(H)reductase (2008)
  • Author(s): Hanke, G.T., Endo, T., Satoh, F. and Hase, T.
  • Journal Title: Plant Cell Environment 31 Pages: 1017-1028
• [Journal Article] NDF6 ; a thylakoid protein specific to terrestrial plants is a novel subunit of chloroplastic NAD(P)H dehydrogenase in Arabidopsis (2008)
  • Author(s): Ishikawa, N., Takabayashi, A., Ishida, S., Hano, Y., Endo, T. and Sato, F.
  • Journal Title: Plant Cell Physiol 49 Pages: 1066-1073
• [Journal Article] electron transport activities of Arabidopsis mutants with impaired chloroplastic NAD(P)H dehydrogenase (2008)
  • Author(s): Ishikawa, N, Endo, T. and Sato, F.
  • Journal Title: J. Plant Res 121 Pages: 521-526
• [Journal Article] Lichen photobionts show tolerance against lichen acids produced by lichen mycobionts (2008)
  • Author(s): Takahagi, T., Endo, T., Yamamoto, Y. and Sato, F.
  • Journal Title: Biosci. Biotech. Biochem 72 Pages: 3122-3127
  • NAID: 10027534800
• [Journal Article] Chloroplastic NAD(P)H dehydrogenase complex and cyclic electron transport around photosystem I. (2008)
  • Author(s): Endo, T., Ishida, S., Ishikawa, N., and Sato, F
  • Journal Title: Mol. Cells 25 Pages: 158-162
• [Journal Article] 光合成における電子伝達系の最近の研究から (2008)
  • Author(s): 遠藤 剛, 石田智, 石川規子, 佐藤文彦
  • Journal Title: ~葉緑体の「呼吸」電子伝達鎖 植物の生長調節 43 Pages: 89-94
• [Journal Article] NDF6 : a thylakoid protein specific to terrestrial plants is a novel subunit of chloroplastic NAD(P)H dehydrogenase in Arabidopsis. (2008)
  • Author(s): Ishikawa, N., Takabayashi, A., Ishida, S., Hano, Y., Endo, T., Sato, F.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1066-1073
  • Peer Reviewed
• [Journal Article] Electron transport activities of Arabidopsis mutants with impaired chloroplastic NAD(P)H dehydrogenase. (2008)
  • Author(s): Ishikawa, N, Endo, T., Sato, F.
  • Journal Title: J. Plant Res. 121 Pages: 521-526
  • Peer Reviewed
• [Journal Article] Altered photosynthetic electron channeling into cyclic electronflow and nitrite assimilation in a mutant of ferredoxin : NADP(H)reductase. (2008)
  • Author(s): Hanke, G. T., Endo, T., Satoh, F., Hase, T.
  • Journal Title: Plant Cell Environment 31 Pages: 1017-1028
  • Peer Reviewed
• [Journal Article] Lichen photobionts show tolerance against lichen acids produced by lichen mycobionts. (2008)
  • Author(s): Takahagi, T., Endo, T., Yamamoto, Y., Sato, F.
  • Journal Title: Biosci. Biotech. Biochem. 72 Pages: 3122-3127
  • NAID: 10027534800
  • Peer Reviewed
• [Journal Article] Chloroplastic NAD(P)H dehydrogenase complex and cyclicelectron transport around photosystem I (2008)
  • Author(s): Endo T, Ishida S, Ishikawa, N, Sato F
  • Journal Title: Molecules and Cells 25
• [Presentation] (2009)
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋大学
• [Presentation] (2008)
  • Organizer: 第49回日本植物生理学会
  • Place of Presentation: 札幌コンベンションセンター