Clarification of mechanism of biosynthesis and accumulation of a large amount of ascorbic acid

• Project/Area Number: 19580108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Hiroshima University
• Principal Investigator: ESAKA Muneharu Hiroshima University, 大学院・生物圏科学研究科, 教授 (70151975)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: アスコルビン酸 / アセロラ / 遺伝子発現 / ストレス抵抗性植物 / Cdna / ビタミンC / 酵素 / クローニング / cDNA

Research Abstract

熱帯植物の一つであるアセロラは、特に果実に大量のアスコルビン酸を含む(レモンの20倍以上)。本研究は、植物のアスコルビン酸の増強を目的として、アセロラのアスコルビン酸生合成遺伝子をクローニングし、その発現機構を調べ、アセロラに大量のアスコルビン酸が生合成・集積する機構について明らかにするとともに、アセロラのアスコルビン酸生合成酵素遺伝子を植物に導入発現させ、アスコルビン酸高含量遺伝子組換え植物の作出をめざしたものですある。

Research Products

• [Journal Article] Increase in ascorbate content of transgenic tobacco plants overexpressing the acerola (Malpighia glabra) phosphomannomutase gene (2009)
  • Author(s): Badejo AA, Eltelib HA, Fukunaga K, Fujikawa Y, Esaka M.
  • Journal Title: Plant Cell Physiol 50(2) Pages: 423-428
  • NAID: 10027338001
  • Peer Reviewed
• [Journal Article] Gene expression of ascorbic acid biosynthesis related enzymes of Smirnoff-Wheeler pathway in acerola (Malpighia glabra). J. (2009)
  • Author(s): Badejo A.A, Fujikawa Y, Esaka M.
  • Journal Title: Plant Physiol 166(6) Pages: 652-660
  • Peer Reviewed
• [Journal Article] Gene expression of ascorbic acid biosynthesis related enzymes of the Smirnoff-Wheeler pathway in acerola (Malpighia glabra). (2009)
  • Author(s): Badejo AA, Fujikawa Y, Esaka M.
  • Journal Title: J. Plant Physiol. 166 Pages: 652-660
  • Peer Reviewed
• [Journal Article] Increase in ascorbate content of transgenic tobacco plants overexpressing acerola (Malpighia glabra)phosphomannomutase gene. (2009)
  • Author(s): Badejo AA, Eltelib HA, Fukunaga K, Fujikawa Y, Esaka M.
  • Journal Title: Plant Cell Physiol. 50 Pages: 423-428
  • NAID: 10027338001
  • Peer Reviewed
• [Journal Article] RNAi-mediated knockdown of the XIP-type endoxylanase inhibitor gene, OsXIP, has no effect on grain development and germination in rice (2008)
  • Author(s): Tokunaga T. Miyata Y. Fujikawa Y. Esaka M.
  • Journal Title: Plant Cell Physiol 49(7) Pages: 1122-1127
  • NAID: 10027332664
  • Peer Reviewed
• [Journal Article] Plant catalase is imported into peroxisomes by Pex5p but is distinct from typical PTS1 import (2008)
  • Author(s): Oshima Y. Kamigaki A. Nakamori C. Mano S. Hayashi M. Nishimura M. Esaka M.
  • Journal Title: Plant Cell Physiol 49(4) Pages: 671-677
  • NAID: 10027330794
  • Peer Reviewed
• [Journal Article] Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra) and increase in ascorbate content of transgenic tobacco expressing the acerola gene (2008)
  • Author(s): Badejo A.A, Tanaka N, Esaka M.
  • Journal Title: Plant Cell Physiol 49(1) Pages: 126-132
  • NAID: 10027495430
  • Peer Reviewed
• [Journal Article] Expression of multi-copy flavonoid pathway genes coincides with anthocyanin, flavonol and flavan-3-ol accumulation of grapevine (2008)
  • Author(s): Jeong ST. Goto-Yamamoto N. Hashizume K. Esaka M.
  • Journal Title: Vitis 47 Pages: 135-140
  • Peer Reviewed
• [Journal Article] Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola(Malpighia glabra) and increase in ascorbate content of transgenic tobaccoexpressing the acerola gene. (2008)
  • Author(s): Badejo A. A., Tanaka N., Esaka M.
  • Journal Title: Plant Cell Physiol. 49 Pages: 126-132
  • NAID: 10027495430
  • Peer Reviewed
• [Journal Article] Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra)and increase in ascorbate content oftransgenic tobacco expressing the acerola gene. (2008)
  • Author(s): Badejo A. A., Tanaka N., Esaka M.
  • Journal Title: Plant Cell Physiol. 49 Pages: 126-132
  • Peer Reviewed
• [Journal Article] Induction of a novel XIP-type xylanase inhibitor by external ascorbic acid treatment and differential expression of XIP-family genes in rice (2007)
  • Author(s): Tokunaga T. Esaka M.
  • Journal Title: Plant Cell Physiol 48 Pages: 700-714
  • NAID: 10027001889
  • Peer Reviewed
• [Journal Article] Cloning and expression of GDP-D-mannose pyrophosphorylase gene and ascorbic acid content of acerola (Malpighia glabra L.) fruit at ripening stages (2007)
  • Author(s): Badejo A.A. Jeong S.T. Goto-Yamamoto N. Esaka M.
  • Journal Title: Plant Physiol. Biochem 45 Pages: 665-672
  • Peer Reviewed
• [Journal Article] Molecular cloning and expression of GDP-D-mannose 3", 5"-epimerase during fruit ripening in acerola (2007)
  • Author(s): Badejo A.A. Jeong S.T. Goto-Yamamoto N. Esaka M.
  • Journal Title: Acta Horticulturae 763 Pages: 91-97
  • Peer Reviewed
• [Journal Article] Induction of a novel XIP-type xylanase inhibitor by external ascorbic acid treat ment and differential expression of XIP-family genes in rice. (2007)
  • Author(s): Tokunaga, T., Esaka, M.
  • Journal Title: Plant Cell Physiol. 48 Pages: 700-714
  • NAID: 10027001889
  • Peer Reviewed
• [Journal Article] Cloning and expression of GDP-D-mannose pyrophosphorylase gene and ascorbicacid content of acerola (Malpighia glabra L.) fruit at ripening stages. (2007)
  • Author(s): Badejo, A. A., Jeong S. T., Goto-Yamamoto, N., Esaka, M.
  • Journal Title: Plant Physiol. Biochem. 45 Pages: 665-672
  • Peer Reviewed
• [Presentation] タバコのアスコルビン酸生合成におけるGPPaseの活性制御機構の解明 (2008)
  • Author(s): 坂本真吾、藤川愉吉、江坂宗春
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸市、神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] Engineering Increased Vitamin C Content in Transgenic Tobacco Plants Expressing Two Acerola (Malpighia glabra)Genes. (2008)
  • Author(s): Badejo A. A., Eltelib H. A., Fujikawa Y., Esaka M.
  • Organizer: AHC-2008
  • Place of Presentation: 韓国 済州島
  • Year and Date: 2008-12-11
• [Presentation] トマトにおけるアスコルビン酸生合成遺伝子ガラクツロン酸レダクターゼの遺伝子発現 (2008)
  • Author(s): 坂本真吾、村野亜沙子、藤川愉吉、江坂宗春
  • Organizer: 日本農芸化学会中四国支部第22回講演会
  • Place of Presentation: 鳥取市鳥取大学
  • Year and Date: 2008-09-13
• [Presentation] イネのアスコルビン酸生合成と光応答 (2008)
  • Author(s): 福永一成、寺田龍司、加藤もも、江坂宗春
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋市名城大学
  • Year and Date: 2008-03-27
• [Presentation] タバコにおけるアスコルビン酸生合成マンノース経路遺伝子の発現調節 (2008)
  • Author(s): 坂本真吾、江坂宗春
  • Organizer: 日本農芸化学会2008年度大会
  • Place of Presentation: 名古屋市名城大学
  • Year and Date: 2008-03-27
• [Presentation] Engineering Increased Vitamin C Concentration in Transgenic Tobacco with Acerola (2008)
  • Author(s): Badejo AA, Eltelib HA, Fujikawa Y, Esaka M.
  • Organizer: AHC 2008 (The Asian Horticultural Congress 2008)
  • Place of Presentation: Seogwipo, Korea
• [Presentation] Analysis of the molecular property and expression profile of a novel xylanase inhibitor in rice. (2007)
  • Author(s): Tokunaga T, Miyata Y, Esaka M.
  • Organizer: The 5th International Symposium on the Molecular Breeding of Forage and Turf
  • Place of Presentation: 札幌
  • Year and Date: 2007-07-01
• [Presentation] タバコにおけるアスコルビン酸生合成と生理機能の解明 (2007)
  • Author(s): 坂本真吾、福永一成、江坂宗春
  • Organizer: 日本農芸化学会中四国支部第18回講演会(例会)
  • Place of Presentation: 広島市、県立広島大学広島キャンパス
  • Year and Date: 2007-05-12
• [Presentation] Analysis of the molecular property and expression profile of a novel xylanase inhibitor in rice (2007)
  • Author(s): Tokunaga T. Miyata Y. Esaka M.
  • Organizer: The 5th International Symposium on the Molecular Breeding of Forage and Turf (MBFT)
  • Place of Presentation: Sapporo, Hokkaido, Japan
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp-enzyme-esaka.html