Analysis of molecular basis for aging regulated by cellular senescence programs and its application to development of anti-aging drugs

• Project/Area Number: 19580109
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyushu University
• Principal Investigator: KATAKURA Yoshinori Kyushu University, 大学院・農学研究院, 准教授 (50264106)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 細胞老化 / アンチエイジング / 細胞応答 / シグナル伝達 / プロテオミクス / 個体老化 / shRNA / TAK1 / TOF-MAS / 糖尿病

Research Abstract

若い細胞と老化細胞の間での発現の違いをもとに新奇細胞老化関連因子の同定を行った。本因子群は、細胞老化の進行時ばかりでなく、糖尿病患者由来組織においても高発現しており、新たな加齢性疾患発症を制御しうる因子と考えられた。また細胞老化を規定する因子を含むものであることも明らかになった。さらに、細胞老化因子として同定したTAK1を中心とした細胞老化プログラムのネットワークを明らかにした。

Research Products

• [Journal Article] Pro-senescent effect of hydrogen peroxide on cancer cells and its possible application to tumor suppuression (2009)
  • Author(s): Kaichi Yoshizaki, Tsukasa Fujiki, Takahiro Tsunematsu, Makiko Yamashita, Miyako Udono, Sanetaka Shirahata, Yoshinori Katakura
  • Journal Title: Biosci. Biotechnol. Biochem 73 Pages: 311-315
  • Peer Reviewed
• [Journal Article] Pro-senescent effect of hydrogen peroxide on cancer cells and its possible application to tumor suppuression (2009)
  • Author(s): Kaichi Yoshizaki, et al.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 311-315
  • Peer Reviewed
• [Journal Article] Protein kinase C d plays a key role in cellular senescence programs of human normal diploid cells (2009)
  • Author(s): Yoshinori Katakura, et al.
  • Journal Title: J. Biochem. (in press)
  • Peer Reviewed
• [Journal Article] Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter (2008)
  • Author(s): Miyako Udono, Tsukasa Fujiki, Makiko Yamashita, Kazuyuki Katsuki, Masaaki Funata Yusuke Imada, Kaichi Yoshizaki, Sanetaka Shirahata, Yoshinori Katakura
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 1638-1641
  • NAID: 10027529237
  • Peer Reviewed
• [Journal Article] Generation of human monoclonal antibodies against Propionibacterium acnes by applying the phage display method to human peripheral blood mononuclear cells immunized in vitro (2008)
  • Author(s): Yeon Suk Jung, Shin-ei Matsumoto, Yoshinori Katakura, Makiko Yamashita, Kosuke Tomimatsu, Shigeru Kabayama, Kiichiro Teruya, Sanetaka Shirahata
  • Journal Title: Cytotechnol 57 Pages: 169-175
  • Peer Reviewed
• [Journal Article] A rapid and efficient strategy to generate antigen-specific human monoclonal antibody by in vitro immunization and the phage display method (2008)
  • Author(s): Shin-ei Matsumoto, Makiko Yamashita, Yoshinori Katakura, Yoshihiro Aiba, Kosuke Tomimatsu, Shigeru Kabayama, Kiichiro Teruya, Sanetaka Shirahata
  • Journal Title: J. Immunol. Methods 332 Pages: 2-9
  • Peer Reviewed
• [Journal Article] Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter (2008)
  • Author(s): Miyako Udono, et al.
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 1638-1641
  • Peer Reviewed
• [Journal Article] Generation of human monoclonal antibodies against Propionibacterium acnes by applying the phage display method to human peripheral blood mononuclear cells immunized in vitro (2008)
  • Author(s): Yeon Suk Jung, et al.
  • Journal Title: Cytotechnology 57 Pages: 169-175
  • Peer Reviewed
• [Journal Article] TAK1 represses transcription of the human telomerase reverse transcriptase gene (2007)
  • Author(s): Tsukasa Fujiki, Takumi Miura, Mariko Maura, Hiroshi Shiraishi, Yusuke Imada, Norihisa Uehara, Kosuke Tashiro, Sanetaka Shirahata, Yoshinori Katakura
  • Journal Title: Oncogene 26 Pages: 5258-5266
  • Peer Reviewed
• [Journal Article] Propionibacterium acnes acts as an adjuvant in in vitro immunization of human peripheral blood mononuclear cells (2007)
  • Author(s): Yeon Suk Jung, Shin-ei Matsumoto, Makiko Yamashita, Kosuke Tomimatsu, Kiichiro Teruya, Yoshinori Katakura, Sanetaka Shirahata
  • Journal Title: Biosci. Biotechnol. Biochem 71 Pages: 1963-1969
  • NAID: 10027517729
  • Peer Reviewed
• [Journal Article] Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells (2007)
  • Author(s): Makiko Yamashita, Yoshinori Katakura, Yoshihiro Aiba, Shin-ei Matsumoto, Kazuko Morihara, Kiichiro Teruya, Akira Ichikawa, Sanetaka Shirahata
  • Journal Title: Cytotechnol 55 Pages: 71-77
  • Peer Reviewed
• [Journal Article] Recent advances in the generation of human monoclonal antibody (2007)
  • Author(s): Makiko Yamashita, Yoshinori Katakura, Sanetaka Shirahata
  • Journal Title: Cytotechnol 55 Pages: 55-60
• [Journal Article] Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells (2007)
  • Author(s): Takashi Tamura, Kosuke Tomimatsu, Yoshinori Katakura, Makiko Yamashita, Shin-ei Matsumoto, Yoshihiro Aiba, Yeon Suk Jung, Yoshiichi Abe, Tsukasa Fujiki, Kiichiro Teruya, Sanetaka Shirahata
  • Journal Title: Biosci. Biotechnol. Biochem 71 Pages: 2871-2875
  • NAID: 10027521641
  • Peer Reviewed
• [Journal Article] TAK1 represses transcription of the human telomerase reverse transcriptase gene (2007)
  • Author(s): Tsukasa Fujiki, et. al.
  • Journal Title: Oncogene 26 Pages: 5258-5266
  • Peer Reviewed
• [Journal Article] Propionibacterium acnes acts as an adjuvant in in vitro immunization of human peripheral blood mononuclear cells (2007)
  • Author(s): Yeon Suk Jung, et. al.
  • Journal Title: Biosci, Biotechnol.Biochem. 71 Pages: 1963-1969
  • Peer Reviewed
• [Journal Article] Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells (2007)
  • Author(s): Takashi Tamura, et. al.
  • Journal Title: Biosci.Biotechnol.Biochem. 71 Pages: 2871-2875
  • Peer Reviewed
• [Journal Article] Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells
  • Author(s): Yoshinori Katakura, Miyako Udono, Kazuyuki Katsuki, Hisaya Nishide, Yukiko Tabira, Takahiro Ikei, Makiko Yamashita, Tsukasa Fujiki, Sanetaka Shirahata
  • Journal Title: J. Biochem (in press)
  • NAID: 10025187590
  • Peer Reviewed
• [Presentation] ヒト幼若好塩基球KU812Fを用いた抗アレルギー乳酸菌の探索とその作用機構の解析 (2009)
  • Author(s): 小磯綾加ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] AID活性化乳酸菌の探索と感染防御への応用 (2009)
  • Author(s): 後藤浩予ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] MAPKp38による細胞老化制御の分子基盤 (2009)
  • Author(s): 小畠規寛ら
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] 新規細胞老化因子の同定と生活習慣病発症への寄与の解析 (2009)
  • Author(s): 鵜殿美弥子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
• [Presentation] MAPK p38による細胞老化制御の分子基盤 (2009)
  • Author(s): 小畠規寛
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
• [Presentation] 細胞老化制御ネットワークの分子基盤の解析 (2009)
  • Author(s): 児玉龍彦
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
• [Presentation] テロメラーゼ制御によるアンチエイジングの分子基盤の解析 (2009)
  • Author(s): 小川香織
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡
• [Presentation] ヒト幼若好塩基球様細胞に対する乳酸菌の抗アレルギー効果 (2008)
  • Author(s): 小磯綾加ら
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] AIDプロモーターを活性化する乳酸菌の同定 (2008)
  • Author(s): 後藤浩予ら
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Expression analysis of senescence-associated genes : their possible involvement in thediabetes (2008)
  • Author(s): M. Udonoら
  • Organizer: JAACT2008
  • Place of Presentation: 福岡
  • Year and Date: 2008-11-26
• [Presentation] 体外免疫法を用いたヒト免疫賦活作用を有する乳酸菌の検索 (2008)
  • Author(s): 山下万貴子ら
  • Organizer: 日本食品免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-14
• [Presentation] TAK1依存的に細胞老化を誘導するシグナルネットワークに関する研究 (2008)
  • Author(s): 児玉龍彦
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] MAPキナーゼp38によるhTERT転写抑制の分子機構 (2008)
  • Author(s): 小畠規寛
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] 新規細胞老化関連因子の発現・機能解析 (2008)
  • Author(s): 鵜殿美弥子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] テロメラーゼ制御による延命の分子基盤の解明 (2008)
  • Author(s): 小川香織
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Expression analysis of senescence-associated genes : their possible involvement in the diabetes (2008)
  • Author(s): Miyako Udono
  • Organizer: The 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology
  • Place of Presentation: Fukuoka
• [Presentation] 浸透圧ストレスによるマウスTERT遺伝子の転写制御機構の解析 (2008)
  • Author(s): 藤木司
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
• [Presentation] 新規細胞老化関連因子の同定と発現・機能解析 (2008)
  • Author(s): 鵜殿美弥子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
• [Presentation] 細胞老化プログラムを規定するPKC-δの機能解析 (2008)
  • Author(s): 香月一志
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
• [Presentation] 細胞老化プログラムにおけるTAK1の機能的役割 (2008)
  • Author(s): 児玉龍彦
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
• [Presentation] NFAT5によるマウスTERT遺伝子の転写制御機構の解析 (2007)
  • Author(s): 藤木司
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] MAPキナーゼp38によるテロメラーゼ触媒サブユニット遺伝子転写抑制の分子機構 (2007)
  • Author(s): 中原ゆり
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] 細胞老化を規定する因子としてのTAK1の機能解析 (2007)
  • Author(s): 白水祐輔
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] 新規細胞老化関連因子の同定と発現・機能解析 (2007)
  • Author(s): 鵜殿美弥子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] マウスTERT遺伝子の転写制御機構の解析 (2007)
  • Author(s): 藤木司
  • Organizer: 日本動物細胞工学会大会
  • Place of Presentation: 高崎
• [Remarks]
  • URL: http://web.me.com/katakura/Site/TOP.html
• [Patent(Industrial Property Rights)] 延命効果物質、および感染防御効果・ワクチン効果促進物質、前期物質の検定用コンストラクト、並びにそれらの用途 (2007)
  • Inventor(s): 片倉喜範、白畑實隆、照屋輝一郎、山下万貴子、松本貴之、長谷川隆則
  • Industrial Property Rights Holder: 片倉喜範、白畑實隆、照屋輝一郎、山下万貴子、松本貴之、長谷川隆則
  • Industrial Property Number: 2007-034115
  • Filing Date: 2007-02-14