Physiological role of a novel protein disulfide isomerase present in rice endosperm tissue

• Project/Area Number: 19580110
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Yamaguchi Prefectural University
• Principal Investigator: OGAWA MASAHIRO (OGAWA Masahiro) Yamaguchi Prefectural University, 共通教育機構, 教授 (10160772)
• Co-Investigator(Kenkyū-buntansha): KUMAMARU Toshihiro 九州大学, 大学院・農学研究科, 准教授 (00284555)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: イネ / 胚乳 / PDI / 突然変異 / MNU / 分子シャペロン / RNAi組換体 / プロテインボデイ / PDI2,3 / RNAi組換え体

Research Abstract

It was elucidated that a nobel PDI2;3 with 40 kDa apparent molecular size plays a physiological role for inter-molecular S-S bond formation in rice endosperm tissue. Generally 11S globulin has intramolecular S-S bonding which is catalysed by PDI1;1. with 60 kDa apparent molecular size. However PDI2;3with small 40 kDa apparent molecular size does not function as intramolecular S-S bond forming enzyme. In rice endosperm tissue there are two types of storage proteins such as acid-soluble glutelin and alcohol-soluble prolamin. It has been well known that an intramolecular S-S bonding of proglutelin is formed before being transporting into vacuole. However it has not been known how prolamin aggregate are formed and are accumulated in ER lumen to form PBI. Finally PDI2;3 functions as an enzyme to form intermolecular S-S bond between prolamin polypeptides and which become prolamin aggregate to form PBI.

Research Products

• [Journal Article] A Role for the Cysteine-Rich 10 kDa Prolamn in Protein Body I Formation in Rice. (2011)
  • Author(s): Nagamine, A., H. Matsusaka, T. Ushijima, Y. Kawagoe, M. Ogawa, T.W. Okita, T. Kumamaru.
  • Journal Title: Plant Cell Physiology 印刷中
  • Peer Reviewed
• [Journal Article] Distinct Role of PDI and P5 Sulfhydryl Oxidoreductases in Multiple Pathways for Oxidation of Structurally Diverse Storage Proteins in Rice. (2011)
  • Author(s): Yayoi Onda, A. Nagamine, M. Sakurai, T. Kumamaru, M. Ogawa, Y. Kawagoe
  • Journal Title: Plant Cell 13 Pages: 1-14
  • Peer Reviewed
• [Journal Article] Protein Disulfide Isomerase Like 1-1 Participates in the Maturation of Proglutelin Within the Endoplasmic Reticulum in Rice Endosperm. (2010)
  • Author(s): Mio Satoh-Cruz, A.J. Crofts, Y. Takemoto-Kuno, A. Sugino, H. Washida, N. Crofts, T.W. Okita、M. Ogawa, H. Satoh, T. Kumamaru
  • Journal Title: Plant Cell Physiology 51 Pages: 1581-1593
  • NAID: 10027352794
  • Peer Reviewed
• [Journal Article] Gene-gene Interactions between Mutants that Accumulate Abnormally High Amounts of Proglutelin in Rice Seed. (2010)
  • Author(s): Yuka Ueda, M. Satoh-Cruz, H. Matsusaka, Y. Takemoto-Kuno, M. Fukuda, T.W. Okita, M. Ogawa, H. Satoh, T. Kumamaru
  • Journal Title: Breeding Science 60 Pages: 568-574
  • NAID: 10027121240
  • Peer Reviewed
• [Journal Article] Protein Disulfide Isomerase Like 1-1 Participates in the Maturation of Proglutelin Within the Endoplasmic Reticulum in Rice Endosperm. (2010)
  • Author(s): Mio Satoh-Cruz, A.J.Crofts, Y.Takemoto-Kuno, A.Sugino, H.Washida, N.Crofts, T.W.Okita, M.Ogawa, H.Satoh, T.Kumamaru
  • Journal Title: Plant Cell Physiology Volume: 51 Pages: 1581-1593
  • NAID: 10027352794
  • Peer Reviewed
• [Journal Article] Gene-gene Interactions between Mutants that Accumulate Abnormally High Amounts of Proglutelin in Rice Seed. (2010)
  • Author(s): Yuka Ueda, M.Satoh-Cruz, H.Matsusaka, Y.Takemoto-Kuno, M.Fukuda, T.W.Okita, M.Ogawa, H.Satoh, T.Kumamaru
  • Journal Title: Breeding Science Volume: 60 Pages: 568-574
  • NAID: 10027121240
  • Peer Reviewed
• [Journal Article] Glup4 Gene Encodes Small GTPase Rab5a in Rice. (2010)
  • Author(s): Mio Satoh-Cruz, M.Fukuda, M.Ogawa, H.Satoh, T.Kumamaru
  • Journal Title: Rice Genetics Newsletter Volume: 25 Pages: 48-49
  • Peer Reviewed
• [Journal Article] Vacuolar Processing Enzyme Plays an Essential Role in the Crystalline Structure of Glutelin in Rice Seed. (2010)
  • Author(s): Toshihiro Kumamaru, Y.Uemura, Y.Inoue, Y.Takemoto-Kuno, S.U.Siddiqui, M.Ogawa, I.Hara-Nishimura, H.Satoh
  • Journal Title: Plant Cell Physiology Volume: 51 Pages: 38-46
  • NAID: 130006992651
  • Peer Reviewed
• [Journal Article] Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed (2010)
  • Author(s): Kumamaru T, Y. Uemura, Y. Inoue, Y. Takemoto M. Ogawa, I Hara-Nishimura, H. Satoh
  • Journal Title: Plant Cell Physiology 51 Pages: 38-46
  • NAID: 130006992651
• [Journal Article] Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed (2010)
  • Author(s): Kumamaru T., Y.Uemura, Y.Inoue, Y.Takemoto, S.U.Siddiqui, M.Ogawa, I.Hara-Nishimura, H.Satoh
  • Journal Title: Plant and Cell Physiology 51 Pages: 38-46
  • NAID: 130006992651
  • Peer Reviewed
• [Presentation] 液胞プロセシング酵素はイネ種子グルテリンの結晶構造形成に寄与する (2010)
  • Author(s): 熊丸敏博
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-18
• [Presentation] 液胞プロセッシング酵素はイネ種子グルテリンの結晶構造形成に寄与する (2010)
  • Author(s): 熊丸敏博, 井上佳美, 西村いくこ, 竹本陽子, 小川雅広, 佐藤光
  • Organizer: 第51回植物生理学会年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-18
• [Presentation] 低システインプロラミンの集積制御に関わるEsp1遺伝子はeucaryotic Release Factor 1(eRF1)をコードする (2009)
  • Author(s): 牛島智一, 川越靖, 小川雅広, 佐藤光, 熊丸敏博
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-23
• [Presentation] 低システインプロラミンの集積にかかわるEsp1遺伝子は eucaryotic Release Factor 1 (eRF1) をコードする (2009)
  • Author(s): 牛島智一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-20
• [Presentation] The Role of Cystein-rich Prolamines for the Formation of Prolamine Protein Body in Rice. (2009)
  • Author(s): 長嶺愛
  • Organizer: Plant Biology
  • Place of Presentation: Hawai
• [Presentation] Vacuolar processing enzyme plays an essential role in the formation of the glutelin crystalline structure in rice seed (2009)
  • Author(s): Kumamaru T.
  • Organizer: Plant Biology
  • Place of Presentation: Hawaii
• [Presentation] The small GTPase Rab5a is essential for intracellular transport of glutelin precursor from Golgi apparatus and endosomal membrane organization in developing rice endosperm (2009)
  • Author(s): Fukuda M.
  • Organizer: Plant Biology
  • Place of Presentation: Hawaii
• [Presentation] The role of cystein-rich prolamines for the formation of prolamine protein body in rice (2009)
  • Author(s): Nagamine I.
  • Organizer: Plant Biology
  • Place of Presentation: Hawaii
• [Presentation] Vacuolar processing enzyme plays an essential role in the formation of the glutelin crystalline structure in rice seed (2009)
  • Author(s): 熊丸敏博, 竹本陽子, 西村いくこ, 小川雅広, 佐藤光
  • Organizer: Plant Biology 2009
  • Place of Presentation: ハワイコンベンションセンター(ホノルル)
• [Presentation] The small GTPase Rab5a is essential for intracellular transport of glutelin precursor from Golgi apparatus and endosomal membrane organization in developing rice endosperm (2009)
  • Author(s): Fukuda M., M.Satoh, H.Washida, T.W.Okita, Y.Kawagoe, M.Ogawa, H.Satoh, T.Kumamaru
  • Organizer: Plant Biology 2009
  • Place of Presentation: ハワイコンベンションセンター(ホノルル)
• [Presentation] The role of cysteine-rich prolamines for the formation of prolamine protein body in rice (2009)
  • Author(s): Nagamine, A., Y.Inoue, M.Ogawa, H.Matsusaka, T.Ushijima, H.Satoh, T.W.Okita, T.Kumamaru
  • Organizer: Plant Biology 2009
  • Place of Presentation: ハワイコンベンションセンター(ホノルル)
• [Presentation] イネ種子貯蔵タンパク質の分別輸送・蓄積におけるPDI1 ; 1およびPDI2 ; 3の機能解析 (2008)
  • Author(s): 恩田弥生
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-29
• [Presentation] イネ種子貯蔵タンパク質の分別輸送・蓄積におけるPDI1-1およびPDI2, 3の機能解析 (2008)
  • Author(s): 恩田弥生
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-29
• [Presentation] イネ種子貯蔵タンパク質の突然変異体esp2の優れた加工特性 (2008)
  • Author(s): 川越靖
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-29
• [Presentation] イネ種子貯蔵蛋白質の分別集積・輸送におけるPDIL-1-1およびPDIL2-3の機能解析 (2008)
  • Author(s): 恩田 弥生、 藤本 若菜、 小川 雅広、熊丸 敏博、 川越 靖
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-03-29
• [Presentation] イネ種子貯蔵タンパク質の突然変異体esp2優れた加工特性 (2008)
  • Author(s): 川越 靖、 恩田 弥生、 高星 千恵美、 佐藤 光、 熊丸 敏博
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-03-29
• [Presentation] イネプロラミンPBの核構造形成における10kDaプロラミンの役割 (2008)
  • Author(s): 長嶺愛
  • Organizer: 日本植物生理学会
  • Place of Presentation: 北海道
  • Year and Date: 2008-03-22
• [Presentation] イネ種子貯蔵タンパク質のジスルフィド結合形成の電子伝達系の可視化から見たタンパク質分別輸送・集積の分子機構 (2008)
  • Author(s): 恩田弥生
  • Organizer: 日本植物生理学会
  • Place of Presentation: 北海道
  • Year and Date: 2008-03-22
• [Presentation] イネグルテリンの細胞内輸送と蓄積を制御する遺伝的メカニズム (2008)
  • Author(s): 熊丸敏博
  • Place of Presentation: 北海道
  • Year and Date: 2008-03-22
• [Presentation] イネ種子貯蔵タンパク質の突然変異体esp2における米粉の製パン特性 (2008)
  • Author(s): 川越靖
  • Organizer: 日本植物生理学会
  • Place of Presentation: 北海道
  • Year and Date: 2008-03-22
• [Presentation] イネプロラミンPBの核構造形成における10kDaプロラミンの役割 (2008)
  • Author(s): 長嶺 愛、小川 雅広、 松阪 弘明、 牛島 智一、 佐藤 光、 熊丸 敏博
  • Organizer: 日本植物生理学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2008-03-22
• [Presentation] イネ種子貯蔵タンパク質のジスルフィド結合形成の電子伝達系の可視化から見たタンパク質分別輸送・集積の分子機構 (2008)
  • Author(s): 恩田 弥生、 長嶺 愛. 小川 雅広、 熊丸 敏博、 川越 靖
  • Organizer: 日本植物生理学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2008-03-22
• [Presentation] イネグルテリンの細胞内輸送と蓄積を制御する遺伝的メカニズム (2008)
  • Author(s): 熊丸 敏博、 小川 雅広、 Okita、 TW、 佐藤 光
  • Organizer: 日本植物生理学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2008-03-22
• [Presentation] イネ種子貯蔵タンパク質の突然変異体esp2における米粉の製パン特性 (2008)
  • Author(s): 川越 靖, 高星 千恵美、 恩田 弥生、 熊丸 敏博、 佐藤 光
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2008-03-22
• [Book] 種子の科学とバイオテクノロジー(原田久也監修)(「イネ種子貯蔵タンパク質集積の遺伝制御機構」) (2009)
  • Author(s): 熊丸敏博、小川雅広、佐藤光、T.W. Okita
• [Book] 種子の科学とバイオテクノロジー (原田久也監修) イネ種子貯蔵タンパク質集積の遺伝的制御機構 (2009)
  • Author(s): 熊丸敏博, 小川雅広, 佐藤光, T. W. Okita
• [Book] 種子の科学とバイオテクノロジー(原田久也監修)イネ種子貯蔵タンパク質集積の遺伝的制御機構 (2009)
  • Author(s): 熊丸敏博, 小川雅広, 佐藤光T. W. Okita
  • Publisher: 学会出版センター
• [Book] Plant Cell Monographs (8) Odd-Arne("Protein Body Biogenesis in Cereal Endosperm" Springer-Verlag Heidelberg)(Olsen(ed.): Endosperm.) (2007)
  • Author(s): Kumamaru T., Ogawa M., Satoh H., Okita, T.W.
• [Book] Plant Cell Monographs (8) Odd-Arne Olsen (ed.) : Endosperm. "Protein Body Biogebesis in Cereal Endosperm" (2007)
  • Author(s): Kumamaru, T., Ogawa, M., Satoh, H., Okita, T. W.
  • Publisher: Springer-Verlag Heidelberg
• [Book] Plant Cell Monographs(8)Odd-Arne Olsen(ed.):Endosperm."Protein Body Biogebesi in Cereal Endosperm" (2007)
  • Author(s): Kumamaru, T., Ogawa, M., Satoh. H., and Okita, T. W.
  • Total Pages: 18
  • Publisher: Springer-Verlag Heidelberg