Characterization of a novel enzyme involved in the degradation of plant proteoglycans

• Project/Area Number: 19580112
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: National Agricultural Research Organization
• Principal Investigator: KANEKO Satoshi National Agricultural Research Organization, 食品総合研究所食品バイオテクノロジー研究領域, 主任研究員 (90343821)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 酵素利用学 / 糖質関連酵素 / アラビノガラクタン-プロテイン / 糖加水分解酵素 / Streptomyces avermitilis / アラビノピラノシダーゼ / アラビノガラクタンープロテイン

Research Abstract

A novel enzyme, beta-L-arabinopyranosidase, that liberates arabinose from thickening agent arabinogalactan was isolated from Streptomyces avermitilis, and the three dimentional structure was resolved. The enzyme belongs to the glycoside hydrolase family as same as alpha-D-galactosidases. One aspartic acid in the catalytic pocket of alpha-D-galactosidase changed to glutamic acid in that of beta-L-arabinopyranosidase, and the residue modulates the enzyme activity. Addifionally, it has an arabinopyranose-binding module.

Research Products

• [Journal Article] A beta-L-arabinopyranosidase from Streptomyces avermitilis is a novel member of glycoside hydrolase family (2009)
  • Author(s): Hitomi Ichinose, Zui Fujimoto, Mariko Honda, Koichi Harazono, Atsuko Uzura, Satoshi Kaneko
  • Journal Title: J. Biol. Chem 284 Pages: 25097-25106
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of beta-L-arabinopyranosidase from Streptomyces avermitilis NBRC14893. (2009)
  • Author(s): Zui Fujimoto, Hitomi Ichinose, Koichi Harazono, Mariko Honda, Atsuko Uzura, Satoshi Kaneko
  • Journal Title: Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun 65 Pages: 632-634
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of beta-L-arabinopyranosidase from Streptomyces avermitilis NBRC14893 (2009)
  • Author(s): Fujimoto Z, Ichinose H, Harazono K, Honda M, Uzura A, Kaneko S.
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun 65(6) Pages: 632-634
  • Peer Reviewed
• [Journal Article] A beta-1-Arabinopyranosidase from Streptomyces avermitilis is a novel member of glycoside hydrolase family 27 (2009)
  • Author(s): Ichinose H, Fujimoto Z, Honda M, Harazono K, Nishimoto Y, Uzura A, Kaneko S.
  • Journal Title: J Biol Chem 284(37) Pages: 25097-25106
  • Peer Reviewed
• [Presentation] 放線菌由来β-L-アラビノピラノシダーゼの結晶構造 (2009)
  • Author(s): 藤本瑞、一ノ瀬仁美、原園幸一、卯津羅淳子、平井雅寛、○金子哲
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡マリンメッセ
  • Year and Date: 2009-03-29
• [Presentation] 放線菌由来β-L-アラビノピラノシダーゼ (2009)
  • Author(s): 一ノ瀬仁美、藤本瑞、原園幸一、卯津羅淳子、平井雅寛、○金子哲
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡マリンメッセ
  • Year and Date: 2009-03-29
• [Presentation] Structural and functional analysis of beta-L-arabinopyranosidase from Streptomyces avermitilis. (2009)
  • Author(s): Hitomi Ichinose, Zui Fujimoto, Mariko Honda, Koichi Harazono, Yukifumi Nishimoto, Atsuko Uzura, ○Satoshi Kaneko
  • Organizer: 2009 Gordon Research Conference (Cellulosomes, Cellulases & Other Carbohydrate Modifying Enzymes)
  • Place of Presentation: Andover (U.S.A. )
• [Presentation] 放線菌のアラビノガラクタン-プロテイン分解酵素 (2008)
  • Author(s): 一ノ瀬仁美、○金子哲
  • Organizer: 日本植物生理学会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-21
• [Patent(Industrial Property Rights)] β-L-アラビノピラノシダーゼ (2008)
  • Inventor(s): 西本幸史、金子哲、一ノ瀬仁美
  • Industrial Property Rights Holder: 長瀬産業株式会社、独立行政法人農業・食品産業技術総合研究機構
  • Industrial Property Number: 2008-310993
  • Filing Date: 2008-12-05
• [Patent(Industrial Property Rights)] β-L-アラビノピラノシダーゼ (2007)
  • Inventor(s): 金 子哲, 他
  • Industrial Property Rights Holder: 独立行政法人農業・食品産業技術総合研究機構
  • Filing Date: 2007-12-05