Characterization of a novel enzyme involved in the degradation of plant proteoglycans
| Project/Area Number |
19580112
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
KANEKO Satoshi National Agricultural Research Organization, 食品総合研究所食品バイオテクノロジー研究領域, 主任研究員 (90343821)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 酵素利用学 / 糖質関連酵素 / アラビノガラクタン-プロテイン / 糖加水分解酵素 / Streptomyces avermitilis / アラビノピラノシダーゼ / アラビノガラクタンープロテイン |
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| Research Abstract |
A novel enzyme, beta-L-arabinopyranosidase, that liberates arabinose from thickening agent arabinogalactan was isolated from Streptomyces avermitilis, and the three dimentional structure was resolved. The enzyme belongs to the glycoside hydrolase family as same as alpha-D-galactosidases. One aspartic acid in the catalytic pocket of alpha-D-galactosidase changed to glutamic acid in that of beta-L-arabinopyranosidase, and the residue modulates the enzyme activity. Addifionally, it has an arabinopyranose-binding module.
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Report
(4 results)
Research Products
(10 results)
