Insect herbivore-produced elicitors of plant defense reactions

• Project/Area Number: 19580122
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Kyoto University
• Principal Investigator: MORI Naoki Kyoto University, 大学院・農学研究科, 准教授 (30293913)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生物活性物質 / 植物の抵抗性の誘導 / Volicitin / エリシター / 窒素代謝 / グルタミン代謝 / グルタミン合成酵素 / 害虫管理 / 植物の誘導抵抗性 / コオロギ / ショウジョウバエ

Research Abstract

Since the first fatty acid amino acid conjugate (FAC) was isolated from regurgitant of Spodoptera exigua larvae, their role as elicitors of induced responses in plants has been well documented. However, studies of the biosyntheses as well as the physiological role of FACs in the insect have been minimal. By utilizing ^<14>C-labeled glutamine, glutamic acid and linolenic acid in feeding studies of S.litura larvae, combined with tissue analyses, we found that FACs play an active role in nitrogen assimilation in Lepidoptera larva and that glutamine containing FACs in the gut lumen may function as a form of storage of glutamine.

Research Products

• [Journal Article] Phospholipid biosynthesis in the gut of Spodoptera litura larvae and effects of tannic acid ingestion (2010)
  • Author(s): Aboshi T, Yoshinaga N, Nishida R, Mori N.
  • Journal Title: Insect Biochem. Mol. Biol. 40 Pages: 325-330
• [Journal Article] Fatty acid-amino acid conjugates diversification in lepidopteran caterpillars (2010)
  • Author(s): Yoshinaga N, Alborn HT, Nakanishi T, Suckling DM, Nishida R, Tumlinson JH, Mori N.
  • Journal Title: J. Chem. Ecol. 36 Pages: 319-325
• [Journal Article] Fatty acid-amino acid conjugates diversification in lepidopteran caterpillars (2010)
  • Author(s): Yoshinaga N, Alborn HT, Nakanishi T, Suckling DM, Nishida R, Tumlinson JH, Mori N
  • Journal Title: Journal of Chemical Ecology 36 Pages: 319-325
  • Peer Reviewed
• [Journal Article] Phospholipid biosynthesis in the gut of Spodoptera litura larvae and effects of tannic acid ingestion (2010)
  • Author(s): Aboshi T, Yoshinaga N, Nishida R, Mori N
  • Journal Title: Insect Biochemistry and Molecular Biology 40 Pages: 325-330
  • Peer Reviewed
• [Journal Article] Efficient incorporation of free oxygen into volicitin in common cutworm Spodoptera litura larvae (2009)
  • Author(s): Ishikawa C, Yoshinaga N, Aboshi T, Nishida R, Mori N.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 1883-1885
• [Journal Article] Species specific glucosylation of DIMBOA in larvae of the rice armyworm. (2009)
  • Author(s): Sasai H, Ishida M, Murakami K, Tadokoro N, Ishihara A, Nishida R, Mori, N.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 1333-1338
  • NAID: 10027542320
• [Journal Article] Species-specific glucosylation of DIMBOA in larvae of the rice armyworm (2009)
  • Author(s): Sasai H, Ishida M, Murakami K, Tadokoro N, Nishida R, Mori N
  • Journal Title: Biosciense Biotechnology and Biochemistry 73 Pages: 1333-1338
  • NAID: 10027542320
  • Peer Reviewed
• [Journal Article] Efficient incorporation of free oxygen into volicitin in common cutworm Spodoptera litura larvae (2009)
  • Author(s): Ishikawa C, Yoshinaga N, Aboshi T, Nishida R, Mori N
  • Journal Title: Biosciense Biotechnology and Biochemistry 73 Pages: 1883-1885
  • Peer Reviewed
• [Journal Article] Active role of fatty acid amino acid conjugates in nitrogen metabolism in Spodoptera litura larvae. (2008)
  • Author(s): Yoshinaga N, Aboshi T, Abe H, Nishida R, Alborn HT, Tumlinson JH, Mori N.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 18058-18063
• [Journal Article] Induction of allergic contact dermatitis by astigmatid mite-derived monoterpene, α-acaridial (2008)
  • Author(s): Sasai T, Hirano Y, Maeda S, Matsunaga I, Otsuka A, Morita D, Nishida R, Nakayama H, Kuwahara Y, Sugita M, Mori N.
  • Journal Title: Biochem. Biophys. Res. Commun 375 Pages: 336-340
  • NAID: 110006684570
• [Journal Article] Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone (2008)
  • Author(s): Noge K, Kato M, Mori N, Kataoka M, Tanaka C, Yamasue Y, Nishida R, Kuwahara Y.
  • Journal Title: FEBS J. 275 Pages: 2807-2817
• [Journal Article] Active role of fatty acid amino acid conjugates in nitrogen metabolism in Spodoptera litura larvae (2008)
  • Author(s): Yoshinaga N, Aboshi T, Abe H, Nishida R, Alborn HT, Tumlinson JH, Mori N
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 18058-18063
  • Peer Reviewed
• [Journal Article] Identification of antibody responses to the serotype-nonspecific molecular species of glycopeptidolipids in Mycobacterium avium infection (2008)
  • Author(s): Matsunaga I, Komori T, Ochi A, Mori N, Sugita M
  • Journal Title: Biochem Biophys Res Commun 377 Pages: 165-169
  • Peer Reviewed
• [Journal Article] Induction of allegic contact dermatitis by astigmatid mite-derived monoterpene, alpha-acaridial (2008)
  • Author(s): Sasai T, Hirano Y, Maeda S, Matsunaga I, Otsuka A, Morita D, Nishida R, Nakayama H, Kuwahara Y, Sugita M, Mori N
  • Journal Title: Biochem Biopys Res Commun 375 Pages: 336-340
  • Peer Reviewed
• [Journal Article] Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite, Carpoglyphus lactis (Atari: Carpoglyphidae). (2008)
  • Author(s): Noge K, Kato M, Mori N, KataokaM, Tanaka C, Yamasue Y, NishidaR, Kuwahara Y
  • Journal Title: FEBS Journal (in press)
  • Peer Reviewed
• [Journal Article] Fatty acid amides, previously identified in caterpillars, found in the cricket Teleogryllus taiwanemma and fruit fly Drosophila melanogaster larvae. (2007)
  • Author(s): Yoshinaga N, Aboshi T, Ishikawa C, Fukui M, Shimoda M, Nishida R, Lait CG, Tu, linson JH, Mori N
  • Journal Title: Journal of Chemical Ecology 33 Pages: 1376-1381
  • Peer Reviewed
• [Journal Article] Detection of a neotropical frog alkaloid spiropyrrolizidine 236 from a Japanese polyzoniid millipede Kiusiozonium okai as amajor defense component together with polyzonimine and nitropolyzonamine. (2007)
  • Author(s): Kuwahara Y, Mori N, Tanabe T
  • Journal Title: Japanese Journal of Environmental Entomology and Zoology 18 Pages: 91-95
  • Peer Reviewed
• [Presentation] 植物に抵抗性を誘導する脂肪酸-グルタミン酸縮合物の生合成 (2010)
  • Author(s): 森直樹、吉田哲也、阿部弘明、杉岡弘基、網干貴子、吉永直子、福井昌夫
  • Organizer: 第54回日本応用動物昆虫学会年次大会
  • Place of Presentation: 千葉千葉大学西千葉キャンパス
• [Presentation] 昆虫由来エリシターvolicitinを巡る防御と適応の化学生態学 (2010)
  • Author(s): 森直樹、吉永直子
  • Organizer: 日本農芸化学会2010年度大会(シンポジウム)
  • Place of Presentation: 東京東京大学駒場キャンパス
• [Presentation] カイコ幼虫のリン脂質組成の解析 (2010)
  • Author(s): 網干貴子、小野肇、西田律夫、森直樹
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京東京大学駒場キャンパス
• [Presentation] キイロショウジョウバエにおける脂肪酸-アミノ酸縮合物の同定 (2010)
  • Author(s): 吉田哲也、杉岡弘基、阿部弘明、網干貴子、吉永直子、福井昌夫、小野肇、西田律夫、森直樹
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京東京大学駒場キャンパス
• [Presentation] 植物に抵抗性を誘導する脂肪酸-グルタミン酸縮合物の生合成 (2010)
  • Author(s): 森直樹、吉田哲也、阿部弘明、杉岡弘基、網干貴子、吉永直子、福井昌
  • Organizer: 第54回日本応用動物昆虫学会年次大会
  • Place of Presentation: 千葉大学西千葉キャンパス
• [Presentation] 昆虫由来エリシターvolicitinを巡る防御と適応の化学生態学 (2010)
  • Author(s): 森直樹、吉永直子
  • Organizer: 日本農芸化学会2010年度大会(シンポジウム)
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] カイコ幼虫のリン脂質組成の解析 (2010)
  • Author(s): 網干貴子、小野肇、西田律夫、森直樹
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] キイロショウジョウバエにおける脂肪酸-アミノ酸縮合物の同定 (2010)
  • Author(s): 吉田哲也、杉岡弘基、阿部弘明、網干貴子、吉永直子、福井昌夫、小野肇、西田律夫、森直樹
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学駒場キャンパス
• [Presentation] カイコ幼虫各組織のリン脂質組成の解析 (2009)
  • Author(s): 網干貴子, 小野肇, 西田律夫, 森直樹
  • Organizer: 日本農芸化学会2009年度大学
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] Gryllus属コオロギにおける脂肪酸-アミノ酸縮合物(FACs)の同定 (2009)
  • Author(s): 杉岡弘基, 阿部弘明, 吉永直子, 網干貴子, 石川千裕, 福井昌夫, 竹田真木生, 小野肇, 西田律夫, 森直樹
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] 鱗翅目幼虫唾液成分volicitin類(FACs)の生理機能および多様性 (2009)
  • Author(s): 吉永直子, 網干貴子, 阿部弘明, 西田律夫, Alborn Hans T, Tumlinson James H, 森直樹
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] カイコ幼虫各組織のリン脂質組成の解析 (2009)
  • Author(s): 網干貴子、小野肇、西田律夫、森直樹
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡マリンメッセ福岡
• [Presentation] Gryllus属コオロギにおける脂肪酸-アミノ酸縮合物(FACs)の同定 (2009)
  • Author(s): 杉岡弘基、阿部弘明、吉永直子、網干貴子、石川千裕、福井昌夫、竹田真木生、小野肇、西田律夫、森直樹
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡マリンメッセ福岡
• [Presentation] 鱗翅目幼虫唾液成分volicitin類(FACs)の生理機能および多様性 (2009)
  • Author(s): 吉永直子、網干貴子、阿部弘明、西田律夫、Alborn Hans T、Tumlinson James h、森直樹
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡マリンメッセ福岡
• [Presentation] Mori N Analysis of phopholipids molecular species in the silkworm (Bombyx mori) by LCMS-IT-TOF (2009)
  • Author(s): Aboshi T, Nishida R
  • Organizer: 2009 Annual Meeting of International Society of Chemical Ecology
  • Place of Presentation: University of Neuchatel, Neuchatel, Switzerland
• [Presentation] Tumlinson JH Chemical structure and elicitor activities of fattyacid amino acid conjugates (2009)
  • Author(s): Yoshinaga N, Ishikawa C, Bosak E, Mori N
  • Organizer: The strategies between herbivore and plants, 2009 Annual Meeting of International Society of Chemical Ecology
  • Place of Presentation: University of Neuchatel, Neuchatel, Switzerland
• [Presentation] activity, and evolutionary diversity of fatty acid amino acid conjugates in insects (2009)
  • Author(s): Mori N Function
  • Organizer: 2009 Asia Pacific Conference on Chemical Ecology
  • Place of Presentation: Hilton Waikiki Prince Kuhio, Honolulu, USA
• [Presentation] activity, and evolutionary diversity of fatty acid amino acid conjugates in insects (2009)
  • Author(s): Mori N Function
  • Organizer: 2009 Annual Meeting of The Entomological Society of America
  • Place of Presentation: Indianapolis Convention Center, Indianapolis, USA
• [Presentation] ハスモンヨトウ幼虫腸内のリゾホスファチジルコリン生合成経路の解明 (2009)
  • Author(s): 網干貴子、森直樹
  • Organizer: 第20回日本環境動物昆虫学会年次大会
  • Place of Presentation: 京都京都大学百周年時計台記念館
• [Presentation] Analysis of phopholipids molecular species in the silkworm (Bombyx mori) by LCMS-IT-TOF (2009)
  • Author(s): Aboshi T, Nishida R, Mori N
  • Organizer: 2009 Annual Meeting of International Society of Chemical Ecology
  • Place of Presentation: University of Neuchatel, Neuchatel, Switzerland
• [Presentation] Chemical structure and elicitor activities of fattyacid amino acid conjugates : The strategies between herbivore and plants (2009)
  • Author(s): Yoshinaga N, Ishikawa C, Bosak E, Mori N, Tumlinson JH
  • Organizer: 2009 Annual Meeting of International Society of Chemical Ecology
  • Place of Presentation: University of Neuchatel, Neuchatel, Switzerland
• [Presentation] Function, activity, and evolutionary diversity of fatty acid amino acid conjugates in insects (2009)
  • Author(s): Mori N
  • Organizer: 2009 Asia Pacific Conference on Chemical Ecology
  • Place of Presentation: Hilton Waikiki Prince Kuhio,
• [Presentation] Function, activity, and evolutionary diversity of fatty acid amino acid conjugates in insects (2009)
  • Author(s): Mori N
  • Organizer: 2009 Annual Meeting of The Entomological Society of America
  • Place of Presentation: Indianapolis Convention Center, Indianapolis, USA
• [Presentation] Increased universality of lepidopteran elicitor compounds across insects : Identification of fatty acid amino acid comjugates (2008)
  • Author(s): Yoshinaga N, Abe H, Fukui M, Nishida R, Alborn HT, Lait CG, Tumlinson JH, Mori N
  • Organizer: The 25 International Society of Cemical Ecology Annual Meeting
  • Place of Presentation: Pennsylvania State University
  • Year and Date: 2008-08-18
• [Presentation] 新規volicitin類縁体の同定とエリシター活性 (2008)
  • Author(s): 石川千裕、吉永直子、網干貴子、Lait Cameron、小野肇、西田律夫、Tumlinson Jim、森直樹
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 名古屋名城大学
• [Presentation] タイワンエンマコオロギにおけるN-linolenoyl-L-glutamic acidの同定と生合成経路の解明 (2008)
  • Author(s): 阿部弘明、吉永直子、網干貴子、石川千裕、福井昌夫、小野肇、西田律夫、森直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋名城大学
• [Presentation] ハスモンヨトウにおけるリン脂質代謝の解析 (2008)
  • Author(s): 網干貴子、吉永直子、小野肇、西田律夫、森直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋名城大学
• [Presentation] 新規ダニアレルゲンα-acaridialによるマウス皮膚炎モデルの確立 (2008)
  • Author(s): 平野裕之介、前田清香、松永勇、大塚篤司、森田大輔、西田律夫、中山秀夫、桑原保正、杉田昌彦、森直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋名城大学
• [Presentation] Structure, function, and elicitor activity of fatty acid amides (FAAs) in Lepidopteran caterpillars (2008)
  • Author(s): Yoshinaga N, Ishikawa C, Nishida R, Lait CG, Tumlinson JH, Mori N
  • Organizer: Entomological Society of America Annual Meeting
  • Place of Presentation: The Town and Country Resort and Convention Center, San Diego, California, USA
• [Presentation] Importance of glutamine moiety in volicitin (2008)
  • Author(s): Yoshinaga N, Sawada Y, Nishida R, Mori N
  • Organizer: The 23rd Annual Meeting of the International Society of Chemical Ecology
  • Place of Presentation: Friedrich Schiller University, Jena, Germany
• [Presentation] Volicitin biosynthesis and nitrogen metabolism in Spodoptera litura larvae (2008)
  • Author(s): Yoshinaga N, Nishida R, Mori N
  • Organizer: The 4th Asia-Pacific Conference on Chemical Ecology
  • Place of Presentation: Tsukuba International Congress Center, Tsukuba, Japan
• [Presentation] Increased universality of lepidopteran elicitor compounds across insects: Identification of fatty acid amino acid comjugates (2008)
  • Author(s): Yoshinaga N, Abe H, Fukui M, Nishida R, Alborn HT, Lait CG, Tumlinson JH, Mori N
  • Organizer: The 25 International Society of Cemical Ecology Annual Meeting
  • Place of Presentation: Pennsylvania State University
• [Presentation] Function and evolutionary diversity of fatty acid amino acid conjugates in lepidopteran caterpillars (2008)
  • Author(s): Yoshinaga N, Nishida R, Alborn HT, Tumlinson JH, Mori N
  • Organizer: The 25 International Society of Cemical Ecology Annual Meeting
  • Place of Presentation: Pennsylvania State University
• [Presentation] Phospholipid biosyntheses in Spodoptera litura gut tissues (2008)
  • Author(s): Aboshi T, Yoshinaga N, Nishida R, Mori N
  • Organizer: The 25 International Society of Cemical Ecology Annual Meeting
  • Place of Presentation: Pennsylvania State University
• [Presentation] Identification of novel volicitin-related compounds from Manduca sexta and their elicitor activity (2008)
  • Author(s): Ishikawa C, Yoshinaga N, Aboshi T, Lait CG, Ono H, Nishida R, Tumlinson JH, Mori N
  • Organizer: The 25 International Society of Cemical Ecology Annual Meeting
  • Place of Presentation: Pennsylvania State University
• [Presentation] Induction of allergiccontact dermatitis by astigmatid mite-derived monoterpene (2008)
  • Author(s): Sasai T, Hirano Y, Maeda S, Matsunaga I, Otsuka A, Morita D, Nishida R, Nakayama H, Kuwahara Y, Sugita M, Mori N
  • Organizer: α-acaridial, 2008日韓合同ダニ学会議
  • Place of Presentation: 慶州市韓国
• [Presentation] ハスモンヨトウ幼虫腸内のリゾホスファチジルコリン生合成経路の解明 (2008)
  • Author(s): 網干貴子, 森直樹
  • Organizer: 第20回日本環境動物昆虫学会年次大会
  • Place of Presentation: 京都大学百周年時計台記念館
• [Presentation] Induction of allergiccontact dermatitis by astigmatid mite-derived monoterpene, α-acaridial (2008)
  • Author(s): Sasai T, Hirano Y, Maeda S, Matsunaga I, Otsuka A, Morita D, Nishida R, Nakayama H, Kuwahara Y, Sugita M
  • Organizer: 2008日韓合同ダニ学会議
  • Place of Presentation: 韓国 慶州市
• [Presentation] Structure, function, and elicitor activity of fatty acidamides (FAAs) in Lepidopteran caterpillars, (2008)
  • Author(s): Yoshinaga N, Ishikawa C, NishidaR, LAIT CG, Tumlinson JH, Mori N
  • Organizer: Entomological Society of America Annual Meeting
  • Place of Presentation: The Town and Country Resort and Convention Center, San Diego, California, USA
• [Presentation] 新規volicitin類縁体の同定とエリシター活性 (2008)
  • Author(s): 石川千裕、吉永直子、網干貴子、Lait Cameron、小野 肇、西田律夫、Tumlinson Jim、森 直樹
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 名古屋、名城大学
• [Presentation] タイワンエンマコオロギにおけるN-linolenoyl-L-glutamicacidの同定と生合成経路の解明 (2008)
  • Author(s): 阿部弘明、吉永直子、網干貴子、石川千裕、福井昌夫、小野 肇、西田律夫、森 直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋、名城大学
• [Presentation] ハスモンヨトウにおけるリン脂質代謝の解析 (2008)
  • Author(s): 網干貴子、吉永直子、小野 肇、西田律夫、森 直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋、名城大学
• [Presentation] 新規ダニアレルゲンα-acaridialによるマウス皮膚炎モデルの確立 (2008)
  • Author(s): 平野裕之介、前田清香、松永 勇、大塚篤司、森田大輔、西田律夫、中山秀夫、桑原保正、杉田昌彦、森直樹
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋、名城大学
• [Book] 昆虫・植物間の攻防と植物免疫システムの“界面"、昆虫が拓く未来科学(藤崎憲冶、西田律夫、佐久間正幸編) (2009)
  • Author(s): 森直樹、吉永直子、網干貴子
  • Publisher: 京都大学学術出版会
• [Book] ダニのアルカロイドとヤドクガエル、昆虫が拓く未来科学(藤崎憲冶、西田律夫、佐久間正幸編) (2009)
  • Author(s): 森直樹、桑原保正
  • Publisher: 京都大学学術出版会
• [Book] ダニアレギー最前線、昆虫が拓く未来科学(藤崎憲冶、西田律夫、佐久間正幸編) (2009)
  • Author(s): 森直樹、桑原保正
  • Publisher: 京都大学学術出版会
• [Book] 昆虫科学が拓く未来 第II部第1章 昆虫・植物間の攻防と植物免疫システムの"界面" (2009)
  • Author(s): 森直樹, 吉永直子, 網干貴子
  • Total Pages: 580
  • Publisher: 京都大学学術出版会
• [Book] 昆虫科学が拓く未来 第II部トピックス2 ダニのアルカロイドとヤドクカエル (2009)
  • Author(s): 森直樹, 桑原保正
  • Total Pages: 580
  • Publisher: 京都大学学術出版会
• [Book] 昆虫科学が拓く未来 第III部トピックス3 ダニアレルギー最前線 (2009)
  • Author(s): 森直樹, 桑原保正
  • Total Pages: 580
  • Publisher: 京都大学学術出版会
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• [Remarks]
  • URL: http://www.chemeco.kais.kyoto-u.ac.jp/
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