Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Research Abstract |
We have analyzed the Mg^<2+> transport function of plant chloroplast protein molecules in this study. Plant proteins were expressed in Escherichia coli, and purified using an appropriate detergent. Isolated AtMRS2-10 from Arabidopsis thaliana was reconstituted into liposomes, and Mg^<2+> transport activity into liposomes was assayed using a fluorescent dye and with atomic absorption spectroscopy. This system allows first measurement of Mg^<2+> transport activity of plant protein.
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