Studies on the regulation of plasma homocysteine concentration by protein and amino acid

• Project/Area Number: 19580135
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Shizuoka University
• Principal Investigator: SUGIYAMA Kimio Shizuoka University, 農学部, 教授 (00126781)
• Co-Investigator(Kenkyū-buntansha): MORITA Tatsuya 静岡大学, 農学部, 教授 (90332692)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: タンパク質 / アミノ酸 / ホモシステイン / 動脈硬化 / 栄養学 / ラット

Research Abstract

血漿ホモシステイン(Hcy)濃度は動脈硬化の危険因子として知られている。本研究では、血漿Hcy 濃度に及ぼす食事タンパク質およびアミノ酸の影響をラットを用いて検討した。血漿Hcy 濃度は食事タンパク質の種類や量の影響を受けること、ある種のアミノ酸(Gly,Ser, Cys)は血漿Hcy 濃度を低下させることなどを明らかにするとともに作用機構の解析を行った。これらの研究結果から、血漿Hcy 濃度は食事タンパク質やアミノ酸により制御できることが明確になった。

Research Products

• [Journal Article] Hepatic cystathionine β-synthase activity does not increase in response to methionine supplementation in rats fed a low casein diet : associationwith plasma homocysteine concetrations (2009)
  • Author(s): Ohuchi S, Morita T, Mori M, and Sugiyama K.
  • Journal Title: J. Nutr. Sci. Vitaminol. 55 Pages: 178-185
  • Peer Reviewed
• [Journal Article] Hypohomocysteinemic effect of cysteine is associated with increased plasma cysteine concentration in rats fed diets low in protein and methionine levels (2009)
  • Author(s): Kawakami Y, Ohuchi S, Morita T, and Sugiyama K.
  • Journal Title: J. Nutr. Sci. Vitaminol. 55 Pages: 66-74
  • NAID: 110007110603
  • Peer Reviewed
• [Journal Article] High casein diet decreases plasma homocysteine concentration in rats (2009)
  • Author(s): Ohuchi S, Matsumoto Y, Morita T, and Sugiyama K.
  • Journal Title: J. Nutr. Sci. Vitaminol. 55 Pages: 22-30
  • NAID: 110007110612
  • Peer Reviewed
• [Journal Article] Hepatic cystathionine β-syn-synthase activity does not increase in response to methionine supplementation in rats fed a low casein diet : association with plasma homocysteine concetrations (2009)
  • Author(s): Seiya Ohuchi, et al.
  • Journal Title: Journal of Nutritional Science & Vitaminology 55 Pages: 178-185
  • Peer Reviewed
• [Journal Article] Hypohomocysteinemic effect of cysteine is associated with increased plasma cysteine concentration in rats fed diets low in protein and methionine levels (2009)
  • Author(s): Yoshiko Kawakami, et al.
  • Journal Title: Journal of Nutritional Science & Vitaminology 55 Pages: 66-74
  • Peer Reviewed
• [Journal Article] High casein diet decreases plasma homocysteine concentration in rats (2009)
  • Author(s): Seiya Ohuchi, et al.
  • Journal Title: Journal of Nutritional Science & Vitaminology 55 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Choline deprivation induces hyperhomocysteinemia in rats fed low methionine diets (2008)
  • Author(s): Setoue M, Ohuchi S, Morita T, and Sugiyama K.
  • Journal Title: J. Nutr. Sci. Vitaminol 54 Pages: 483-490
  • NAID: 110007007847
  • Peer Reviewed
• [Journal Article] High-casein diet suppresses guanidinoacetic acid-induced hyperhomocysteinemia and potentiates the hypohomocysteinemic effect of serine in rats (2008)
  • Author(s): Ohuchi S, Matsumoto Y, Morita T, and Sugiyama K.
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 3258-3264
  • NAID: 10027535333
  • Peer Reviewed
• [Journal Article] Effects of various amino acids on methionine-induced hyperhomocysteinemia in rats (2008)
  • Author(s): Fukada S, Morita T, and Sugiyama K.
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 1940-1943
  • NAID: 10027530402
  • Peer Reviewed
• [Journal Article] Hyperhomocysteinemia induced by guanidinoacetic acid is effectively suppressed by choline and betaine in rats (2008)
  • Author(s): Setoue M, Ohuchi S, Morita T, and Sugiyama K.
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 1696-1703
  • NAID: 10027529477
  • Peer Reviewed
• [Journal Article] Effect of dietary soybean protein level on the plasma homocysteine concentration in rats (2008)
  • Author(s): Okawa H, Morita T, and Sugiyama K.
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 1607-1610
  • NAID: 10027529086
  • Peer Reviewed
• [Journal Article] Choline deprivation induces hyperhomocyteinemia in rats fed low methionine diets (2008)
  • Author(s): Minoru Setoue, et al.
  • Journal Title: Journal of Nutritional Science & Vitaminology 54 Pages: 484-491
  • Peer Reviewed
• [Journal Article] High-casein diet suppresses guanidinoacetic acid-induced hyperhomocysteinemia and potentiates the hypohomocysteinemic effect of serine (2008)
  • Author(s): Seiya Ohuchi, et al.
  • Journal Title: Bioscinence, Biotechnology and Biochemistry 72 Pages: 3258-3264
  • Peer Reviewed
• [Journal Article] Effects of various amino acids on methionine-induced hyperhomocysteinemia in rats (2008)
  • Author(s): Shinichiro Fukada, et al.
  • Journal Title: Bioscinence, Biotechnology and Biochemistry 72 Pages: 1940-1943
  • Peer Reviewed
• [Journal Article] Hyperhomocysteinemia induced by guanidinoacetic acid is effectively suppressed by choline and betaine in rats (2008)
  • Author(s): Minoru Setoue, et al.
  • Journal Title: Bioscinence, Biotechnology and Biochemistry 72 Pages: 1696-1703
  • Peer Reviewed
• [Journal Article] Effect of dietary soybean protein level on the plasma homocysteine concentration in rats (2008)
  • Author(s): Hiroshi Okawa, et al.
  • Journal Title: Bioscinence, Biotechnology and Biochemistry 72 Pages: 1607-1610
  • Peer Reviewed
• [Presentation] ベタイン欠乏を伴う高ホモシステン血症に及ぼすメチオニンとセリンの影響 (2009)
  • Author(s): 劉軼群, 他
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎新聞文化ホール
  • Year and Date: 2009-05-21
• [Presentation] 血漿ホモシステイン濃度に及ぼす食餌タンパク質の種類と量の影響 (2009)
  • Author(s): 呉育如, 他
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎新聞文化ホール
  • Year and Date: 2009-05-21
• [Presentation] タンパク質およびアミノ酸摂取量とラット肝臓CBS 活性との関係 (2009)
  • Author(s): 杉山公男, 他
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎新聞文化ホール
  • Year and Date: 2009-05-21
• [Presentation] タンパク質およびアミノ酸摂取量とラット肝臓CBS活性との関係 (2009)
  • Author(s): 杉山公男
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎ブリックホール(長崎市)
  • Year and Date: 2009-05-21
• [Presentation] 血漿ホモシステイン濃度に及ぼす食餌タンパク質の種類と量の影響 (2009)
  • Author(s): 呉育女
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎ブリックホール(長崎市)
  • Year and Date: 2009-05-21
• [Presentation] ベタイン欠乏を伴う高ホモシステイン血症に及ぼす食餌へのメチオニンとセリン添加の影響 (2009)
  • Author(s): 劉軼群
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎ブリックホール(長崎市)
  • Year and Date: 2009-05-21
• [Presentation] 小麦グルテンの血漿ホモシステイン濃度低下作用 (2008)
  • Author(s): 呉育如, 他
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 女子栄養大学坂戸キャンパス
  • Year and Date: 2008-05-04
• [Presentation] 高カゼイン食による血漿ホモシステイン濃度の低下とその機構 (2008)
  • Author(s): 大内誠也, 他
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 女子栄養大学坂戸キャンパス
  • Year and Date: 2008-05-04
• [Presentation] D-システイン投与は血漿ホモシステイン濃度を効果的に低下させる (2008)
  • Author(s): 杉山公男, 他
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 女子栄養大学坂戸キャンパス
  • Year and Date: 2008-05-04
• [Presentation] D-システイン投与はラットの血漿ホモシステイン濃度を効果的に低下させる (2008)
  • Author(s): 杉山 公男
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 女子栄養大学坂戸キャンパス
  • Year and Date: 2008-05-04