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Metabolism of n-3 polyunsaturated fatty acids by lipoxygenases and production of novel bioactive agents

Research Project

Project/Area Number 19580138
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionShimane University

Principal Investigator

JISAKA Mitsuo  Shimane University, 生物資源科学部, 准教授 (60243424)

Co-Investigator(Renkei-kenkyūsha) YOKOTA Kazushige  島根大学, 生物資源科学部, 教授 (90158361)
NAGAYA Tsutomu  島根大学, 生物資源科学部, 准教授 (50284021)
NISHIMURA Kohji  島根大学, 総合科学研究支援センター, 助教 (30304257)
HUKUZAWA Kenji  安田女子大学, 薬学部, 教授
TAKAHASHI Yoshitaka  岡山県立大学, 保険福祉学部, 教授 (10236333)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsリポキシゲナーゼ / n-3系脂肪酸 / ドコサヘキサエン酸 / FOX / 神経保護因子
Research Abstract

Various bioactive agents are biosynthesized from docosahexaenoic acid (DHA). Although lipoxygenases (LOXs) are supposed to be involved in the biosynthetic pathways, details remain to be evaluated. In this research, we examined metabolism of DHA by LOX using purified enzymes and substrates. we focus on human 15-LOX-2, which may be involved in normal function of prostate tissue, and its mouse homologue, 8-LOX. 15-LOX-2 converted DHA solely to 17S-hydroperoxydocosahexaenoic acid (17S-HPDHA), while 8-LOX readily catalyzed double dioxygenation of DHA, producing 10S,17S-diHPDHA as a main product and 7S,17S-diHPDHA as a minor product. 10S,17S-diHPDHA, an isomer of neuroprotectin D1, is reported to suppress aggregation of human platelets. Our results may contribute effective production of some therapeutically significant DHA metabolites.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (16 results)

All 2010 2009 2008 2007 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Up-regulation of adipogenesis in adipocytes expressing stably cyclooxygenase-2 in the antisense direction2010

    • Author(s)
      X. Chu, K. Nishimura, M. Jisaka, T. Nagaya, F. Shono, K. Yokota
    • Journal Title

      Prostaglandins and Other Lipid Mediators 91

      Pages: 1-9

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tumor-Suppressive Lipoxygenase Inhibit the Expression of c-myc mRNA Coding Region Determinant-Binding Protein/Insulin-Like 5 Growth Factor II mRNA-Binding Protein 1 in Human Prostate Carcinoma PC-3 Cells.2009

    • Author(s)
      Y. Kawakami, N. Kubota, N. Ekuni, T. Suzuki-Yamamoto, M. Kimoto, H. Yamashita, H. Tsuji, T. Yoshimoto, M. Jisaka, J. Tanaka, H.F. Fujimura, Y. Miwa, Y. Takahashi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 73

      Pages: 1811-1817

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of enzyme-linked immunosorbent assay for 8-iso-prostaglandin F2(alpha), a biomarker of oxidative stress in vivo, and its application to the quantification in aged rats.2009

    • Author(s)
      X. Chu, Y. Ageishi, K. Nishimura, M. Jisaka, T. Nagaya, F. Shono, K. Yokota
    • Journal Title

      Journal of Pharmacological and Biomedical Analysis 50

      Pages: 911-916

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of adipogenesis program in cultured preadipocytes transfected stably with cyclooxygenase isoforms.2009

    • Author(s)
      X. Chu, L. Xu, K. Nishimura, M. Jisaka, T. Nagaya, F. Shono, K. Yokota
    • Journal Title

      Biochimica et Biophysica Acta 1791

      Pages: 273-280

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of Lipid Hydroperoxides by the Ferric-Xylenol Orange Method (2) Application to Lipoxygenase Assay.2009

    • Author(s)
      K. Fukuzawa, M. Sano, K. Akai, J. Morishige, A. Tokumura, M. Jisaka
    • Journal Title

      Journal of Nutritional Science and Vitaminology 55

      Pages: 92-98

    • NAID

      110007110606

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tumor-Suppressive Lipoxygenase Inhibit the Expression of c-myc mRNA Coding Region Determinant-Binding Protein/Insulin-Like Growth Factor II mRNA-Binding Protein 1 in Human Prostate Carcinoma PC-3 Cells2009

    • Author(s)
      Yuki Kawakami
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 73

      Pages: 1811-1817

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of Lipid Hydroperoxides by the Ferric-Xylenol Orange Method (2) Application to Lipoxygenase Assay2009

    • Author(s)
      Kenji Fukuzawa
    • Journal Title

      Jouranal of Neutritional Science and Vitaminology 55

      Pages: 92-98

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Physiological function of skin-related lipoxygenases2009

    • Author(s)
      Mitsuo Jisaka
    • Organizer
      4th International Conference on Phospholipase A2 and Lipid Mediators (PLM2009)
    • Place of Presentation
      学術総合センター
    • Year and Date
      2009-05-25
    • Related Report
      2009 Final Research Report
  • [Presentation] Physiological function of skin-related lipoxygenases2009

    • Author(s)
      Mitsuo Jisaka
    • Organizer
      4^<th> International Conference on Phopholipase A2 and Lipid Mediators
    • Place of Presentation
      学術総合センター
    • Year and Date
      2009-05-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス8S-リポキシゲナーゼによるドコサヘキサエン酸の酸素添加反応.2008

    • Author(s)
      地阪光生、細川歩、西村浩二、長屋敦、横田一成
    • Organizer
      第50回日本脂質生化学会
    • Place of Presentation
      徳島県郷土文化会館
    • Year and Date
      2008-06-06
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス8S-リポキシゲナーゼによるドコサヘキサエン酸の酸素添加2008

    • Author(s)
      地阪光生
    • Organizer
      日本脂質生化学会
    • Place of Presentation
      徳島県郷土文化会館
    • Year and Date
      2008-06-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Interacting effect of 15-deoxy-Δ^<12>, <14>-prostaglandin J_2 0n the arachidonate cyclooxygenase pathway to generate prostaglandins E_2 and F_<2α>in preadipocytes2008

    • Author(s)
      Abu Asad Chowdhury
    • Organizer
      日本脂質生化学会
    • Place of Presentation
      徳島県郷土文化会館
    • Year and Date
      2008-06-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] 表皮系リポキシゲナーゼによるドコサヘキサエン酸代謝の可能性2008

    • Author(s)
      細川 歩、地阪光生、, 他4名
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス
    • Year and Date
      2008-03-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] 表皮系リポキシゲナーゼによるドコサヘキサエン酸代謝の可能性2008

    • Author(s)
      細川歩、若槻恵、西村浩二、長屋敦、横田一成、地阪光生
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名城大学天白キャンパス(名古屋)
    • Year and Date
      2008-03-26
    • Related Report
      2009 Final Research Report
  • [Presentation] Comparison of the substrate-binding sites of mouse 8-lipoxygenase and human 15-lipoxygenase-2 based on oxygenation of methyl arachidonate and methyl linoleate.2007

    • Author(s)
      M. Jisaka, K. Nishimura, T. Nagaya, K. Yokota
    • Organizer
      The 10th Conference on the Bioactive Lipids in Cancer, Inflammation, and Related Diseases
    • Place of Presentation
      La Centre Sheraton Hotel (Montrteal, Canada)
    • Year and Date
      2007-09-17
    • Related Report
      2009 Final Research Report
  • [Remarks] 島根大学生物資源科学部ホームページ

    • URL

      http://www.life.shimane-u.ac.jp

    • Related Report
      2009 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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