| Project/Area Number |
19580138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Shimane University |
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Principal Investigator |
JISAKA Mitsuo Shimane University, 生物資源科学部, 准教授 (60243424)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOTA Kazushige 島根大学, 生物資源科学部, 教授 (90158361)
NAGAYA Tsutomu 島根大学, 生物資源科学部, 准教授 (50284021)
NISHIMURA Kohji 島根大学, 総合科学研究支援センター, 助教 (30304257)
HUKUZAWA Kenji 安田女子大学, 薬学部, 教授
TAKAHASHI Yoshitaka 岡山県立大学, 保険福祉学部, 教授 (10236333)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | リポキシゲナーゼ / n-3系脂肪酸 / ドコサヘキサエン酸 / FOX / 神経保護因子 |
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| Research Abstract |
Various bioactive agents are biosynthesized from docosahexaenoic acid (DHA). Although lipoxygenases (LOXs) are supposed to be involved in the biosynthetic pathways, details remain to be evaluated. In this research, we examined metabolism of DHA by LOX using purified enzymes and substrates. we focus on human 15-LOX-2, which may be involved in normal function of prostate tissue, and its mouse homologue, 8-LOX. 15-LOX-2 converted DHA solely to 17S-hydroperoxydocosahexaenoic acid (17S-HPDHA), while 8-LOX readily catalyzed double dioxygenation of DHA, producing 10S,17S-diHPDHA as a main product and 7S,17S-diHPDHA as a minor product. 10S,17S-diHPDHA, an isomer of neuroprotectin D1, is reported to suppress aggregation of human platelets. Our results may contribute effective production of some therapeutically significant DHA metabolites.
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