| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
MG-H1, GLAP, argpyrimidine, and 2-(4-acetylamino-4-carboxybutylamino)-5-(5-acetylamino-5-carboxy-pentyl)-7-hydroxy-3, 4-dihydro-pyrido[3,2-d]pyri-midin-5-ium (P4) were identified as AGEs (advanced glycation end products) by the reaction of the N^α-acetyllysine, N^α-acetylarginine, and glyceraldehydes (GLA). Furthermore, glyceraldehyde, glusocone, 3-deoxyglucosone (3-DG), 3-deoxyxylosone, tetrosone, triosone, 3-deoxytetrosone, glyoxal and methylglyoxal were identified by the incubation of glucose or fructose. We found that 3-DG caused apoptosis through the endoplasmic reticulum stress for mouse macrophage cell line, and clarified the significance of the carbonyl stress on the mechanism underlying the pathogenesis of diabetes complication such as arteriosclerosis and ageing. GLAP, and analogs of MG-H1, argpyrimidine, and P4 were also generated from the reaction of GLA, creatine, and N^α-acetyllysine. On the other hand, we revealed that protein modified by GLA suppressed interferon β stimulated gene expression.
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