| Project/Area Number |
19580172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林学・森林工学
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| Research Institution | Kyoto University |
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Principal Investigator |
HATTORI Takefumi Kyoto University, 生存圏研究所, 助教 (60212148)
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| Co-Investigator(Kenkyū-buntansha) |
IWASE Koji 鳥取大学, 農学部, 教授 (60444642)
UMEZAWA Toshiaki 京都大学, 生存圏研究所, 教授 (80151926)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMATO Masahide 鳥取大学, 農学部, 助教 (00571788)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 森林生物 / Laccaria bicolor / シュウ酸 / クエン酸 / リンゴ / リン / 酸性土壌 / 外生菌根菌 / 荒廃地造林 / リンゴ酸 / Fomitopsis palustris / オキサロ酢酸加水分解酵素 / グリオキシル酸脱水素酵素 |
|---|
| Research Abstract |
Ectomycorrhizal (ECM) fungi solubilize water-insoluble minerals containing phosphorus, making phosphate available in soluble form to be taken up by the host tree. Strong chelators, such as oxalate, citrate, and malate, released from ECM play a pivotal role in the dissolution of minerals through complexolysis. In this study, we attempted to clone a cDNA encoding a homologue of the oxalate-producing enzyme cytochrome c dependent glyoxylate dehydrogenase from Laccaria bicolor. We isolated cDNA encoding the cytochrome c dependent glyoxylate dehydrogenase (FpGLOXDH) from Fomitopsis palustris. We found that the homologue of FpGLOXDH (jgi|Lacbil|296363|er2 Lbscfoolog 01720, Laccaria bicolor) was present in the genome of L.bicolor. However, activity of FpGLOXDH was not detected in L.bicolor. Further research is needed to elucidate the mechanism underlying the biosynthesis of these organic acids.
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