Development of transformation system in Lombardy poplar by using a poplar gene as a selective marker gene

• Project/Area Number: 19580178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 林学・森林工学
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: NISHIGUCHI Mitsuru Forestry and Forest Products Research Institute, 生物工学研究領域, 主任研究員 (80353796)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ポプラ / 遺伝子組換え / 選抜マーカー遺伝子 / ツニカマイシン / GPT / バイナリーベクター / 遺伝子発現 / 出芽酵母 / 選抜マーカー

Research Abstract

To obtain a selective marker gene for generation of transgenic trees, we cloned and sequenced the cDNA of UDP-N-acetylglucosamine : dolichyl-phosphate N-acetylglucosaminephosphotransferase (GPT) from Lombardy poplar (Populus nigra var. italica). Overexpression of GPT in budding yeast conferred tunicamycin tolerance. We succeeded in the generation of GPT-introduced transgenic poplars by using tunicamycin and a new binary vector, which contained the cDNA of GPT as a selective marker gene.