Distribution and diversity of sulphate-reducing bacteria in marinesediments as determined using molecular marker

• Project/Area Number: 19580215
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Fukui Prefectural University
• Principal Investigator: KONDO Ryuji Fukui Prefectural University, 生物資源学部, 准教授 (30244528)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 硫酸還元細菌 / 亜硫酸還元酵素遺伝子 / 養殖漁場堆積物 / 有機汚濁 / リアルタイムPCR / PCR-DGGE / 海底堆積物

Research Abstract

本研究では、機能遺伝子をマーカーとして利用し、日本ならびに韓国の沿岸海域の底泥中における硫酸還元細菌の分布生態と多様性を解析した。まず、亜硫酸還元酵素遺伝子を標的とした定量PCR 法による硫酸還元細菌の計数方法を開発した。この方法によって底泥中の硫酸還元細菌を計数したところ、有機物汚濁の進行に伴って硫酸還元細菌が増加することが明らかとなった。PCR-DGGE によってその群集組成を調べた結果、主要な硫酸還元細菌がDesulfobacteraceae 科およびDesulfobulbaceae 科であったが、水域によって硫酸還元細菌の組成が異なることが明らかとなった。

Research Products

• [Journal Article] Dominantbacterioplankton populations in themeromictic Lake Suigetsu as determined bydenaturing gradient gel electrophoresis of16S rRNA gene fragments (2009)
  • Author(s): R. Kondo, A. Nakagawa, L. Mochizuki, K.Osawa, Y. Fujioka and J. Butani
  • Journal Title: Limnology 10(1) Pages: 63-69
• [Journal Article] Comparison of bacterialcommunities in fish farm sediments along anorganic enrichment gradient (2009)
  • Author(s): N. Kawahara, K. Shigematsu, T. Miyadaiand R. Kondo
  • Journal Title: Aquaculture 287 (1-2) Pages: 107-113
• [Journal Article] Comparison of bacterial communities in fish farm sediments along an organic enrichment gradient (2009)
  • Author(s): N. Kawahara, K. Shigematsu, T. Miyadai, R. Kondo
  • Journal Title: Aquaculture 287 Pages: 107-113
  • Peer Reviewed
• [Journal Article] Dominant bacterioplankton populations in the meromictic Lake Suigetsu as determined by denaturing gradient gel electrophoresis of 16S rRNA gene fragments (2009)
  • Author(s): R. Kondo, A. Nakagawa, L. Mochizuki, K. Osawa, Y. Fujioka, J. Butani
  • Journal Title: Limnology 10 Pages: 63-69
  • NAID: 10025405069
  • Peer Reviewed
• [Journal Article] Rapid enumeration of sulphate-reducingbacteria from aquatic environments usingreal-time PCR (2008)
  • Author(s): R. Kondo, K. Shigematsu and J. Butani
  • Journal Title: Plankton & BenthosResearch 3 (3) Pages: 180-183
• [Journal Article] Distribution ofsulfate-reducing bacteria in fish firmsediments (2008)
  • Author(s): N. Kawahara, K. Shigematsu, S. Miura, T.Miyadai and R. Kondo
  • Journal Title: Plankton & Benthos Research 3(1) Pages: 42-45
• [Journal Article] Distribution of sulfate-reducing bacteria in fish firm sediments (2008)
  • Author(s): N. Kawahara, K. Shigematsu, S. Miura, T. Miyadai, R. Kondo
  • Journal Title: Plankton & Benthos Research 3 Pages: 42-45
  • Peer Reviewed
• [Journal Article] Rapid enumeration of sulphate-reducing bacteria from aquatic environments using real-time PCR (2008)
  • Author(s): R. Kondo, K. Shigematsu, J. Butani
  • Journal Title: Plankton & Benthos Research 3 Pages: 180-183
  • NAID: 10021907601
  • Peer Reviewed
• [Journal Article] Rapid enumeration of sulphate-reducing bacteria from aquatic environments usingreal-time PCR (2008)
  • Author(s): R. Kondo, K. Shigematsu and J. Butani
  • Journal Title: Plankton & Benthos Research (掲載確定)
  • Peer Reviewed
• [Journal Article] Distribution of sulfate-reducing bacteria in fish firm sediments (2008)
  • Author(s): N. Kawahara, K. Shigematsu, S. Miura, T. Miyadai and R. Kondo
  • Journal Title: Plankton & Benthos Research 3 Pages: 42-45
  • Peer Reviewed
• [Journal Article] Comparison of thediversity of sulfate-reducing bacterialcommunities in the water column andsurface sediments of a Japanese meromicticlake (2007)
  • Author(s): R. Kondo and J. Butani
  • Journal Title: Limnology 8 (2) Pages: 131-141
• [Journal Article] Spatial dynamics ofsulphate-reducing bacterial compositions insediments along a salinity gradient in a UKestuary (2007)
  • Author(s): R. Kondo, K. J. Purdy, S. Q. Silva and D. B.Nedwell
  • Journal Title: Microbes and Environments 22 (1) Pages: 11-19
• [Journal Article] Comparison of the diversity of sulphate-reducing bacterial communities in the water column and surface sediments of a Japanese meromictic lake (2007)
  • Author(s): R. Kondo and J. Butani
  • Journal Title: Limnology 8 Pages: 131-141
  • Peer Reviewed
• [Journal Article] Spatial dynamics of sulphate-reducing bacterial compositions in sediments along a salinity gradient in a UK estuary (2007)
  • Author(s): R. Kondo, K. J. Purdy, S. Q. Silva and D. B. Nedwell
  • Journal Title: Microbes and Environments 22 Pages: 11-19
  • NAID: 110006242027
  • Peer Reviewed
• [Presentation] T-RFLP 法による魚類養殖漁場底泥中の細菌群集組成の比較 (2009)
  • Author(s): 坂見知子、奥村 裕、村岡大祐、横山 寿、重松孝太朗、近藤竜二
  • Organizer: 平成21 年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] T-RFLP法による魚類養殖漁場底泥中の細菌群集組成の比較 (2009)
  • Author(s): 坂見知子, 奥村裕, 村岡大祐, 横山 寿, 重松幸太朗, 近藤竜二
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-30
• [Presentation] 日本および韓国沿岸域底泥中の硫酸還元細菌の分布と群集組成 (2009)
  • Author(s): 重松孝太朗、近藤竜二、坂見知子、横山寿、小泉喜嗣、尹 良湖
  • Organizer: 平成21 年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] 日本および韓国沿岸域底泥中の硫酸還元細菌の分布と群集組成 (2009)
  • Author(s): 重松幸太朗. 近藤竜二, 坂見知子, 横山 寿, 小泉喜嗣, 尹 良湖
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] 紅色硫黄酸化細菌に特異的なPCR プライマーの開発 (2008)
  • Author(s): 森 裕美、近藤竜二
  • Organizer: 2008年日本プランクトン学会
  • Place of Presentation: 熊本市
  • Year and Date: 2008-09-06
• [Presentation] 紅色硫黄酸化細菌に特異的なPCRプライマーの開発 (2008)
  • Author(s): 森 裕美, 近藤竜二
  • Organizer: 2008年日本プランクトン学会・日本ベントス学会合同大会
  • Place of Presentation: 熊本市
  • Year and Date: 2008-09-06
• [Presentation] Specific detection ofpurple sulfur bacteria using PCR primersthat target dissimilatory sulfite reductasegene (2008)
  • Author(s): Y. Mori and R. Kondo
  • Organizer: 12th International Symposium onMicrobial Ecology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2008-08-21
• [Presentation] Comparison of bacterial communities in fish farm sediments along an organic enrichment gradient (2008)
  • Author(s): R. Kondo, N. Kawahara, K. Shigematsu, T. Miyadai
  • Organizer: 12th International Symposium on Microbial Ecology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2008-08-21
• [Presentation] Comparison of bacterialcommunities in fish farm sediments along anorganic enrichment gradient (2008)
  • Author(s): R. Kondo, N. Kawahara, K. Shigematsu andT. Miyadai
  • Organizer: 12thInternational Symposium on MicrobialEcology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2008-08-19
• [Presentation] Specific detection of purple sulfur bacteria using PCR primers that target dissimilatory sulfite reductase gene (2008)
  • Author(s): Y. Mori, R. Kondo
  • Organizer: 12th International Symposium on Microbial Ecology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2008-08-19
• [Presentation] リアルタイムPCR法による硫酸還元細菌の迅速定量 (2008)
  • Author(s): 近藤竜二、重松孝太朗、部谷巡貴
  • Organizer: 平成20 年度日本水産学会春季大会
  • Place of Presentation: 静岡市
  • Year and Date: 2008-03-28
• [Presentation] リアルタイムPCR法による硫酸還元細菌の迅速定量 (2008)
  • Author(s): 近藤竜二, 重松幸太朗, 部谷巡貴
  • Organizer: 平成20年度日本水産学会春季大会
  • Place of Presentation: 静岡市
  • Year and Date: 2008-03-28
• [Presentation] 光合成硫黄細菌における亜硫酸還元酵素遺伝子の塩基配列の解析 (2008)
  • Author(s): 森 裕美、近藤竜二
  • Organizer: 第24 回日本微生物生態学会
  • Place of Presentation: 札幌市
• [Presentation] 沿岸域底泥中の硫酸還元細菌の分布と群集組成 (2008)
  • Author(s): 重松孝太朗、尹 良湖、近藤竜二
  • Organizer: 第24 回日本微生物生態学会
  • Place of Presentation: 札幌市
• [Presentation] 沿岸域底泥中の硫酸還元細菌の分布と群集組成 (2008)
  • Author(s): 重松孝太朗, 尹良湖, 近藤竜二
  • Organizer: 第24回日本微生物生態学会
  • Place of Presentation: 札幌市
• [Presentation] 光合成硫黄細菌における亜硫酸還元酵素遺伝子の塩基配列の解析 (2008)
  • Author(s): 森 裕美, 近藤竜二
  • Organizer: 第24回日本微生物生態学会
  • Place of Presentation: 札幌市
• [Presentation] PCR法による海面養殖場底泥中の硫酸還元細菌の分布と多様性解析 (2007)
  • Author(s): 重松孝太朗、川原直城、近藤竜二
  • Organizer: 平成19 年度日本水産学会秋季大会
  • Place of Presentation: 函館市
  • Year and Date: 2007-09-26
• [Presentation] PCR法による海面養殖場底泥中の硫酸還元細菌の分布と多様性解析 (2007)
  • Author(s): 重松孝太朗, 川原直城, 近藤竜二
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 函館市
  • Year and Date: 2007-09-26
• [Presentation] Comparison of bacterialcommunities in fish farm sediments withdifferent pollution levels (2007)
  • Author(s): N. Kawahara, K. Shigematsu, T. Miyadaiand R. Kondo
  • Organizer: 第23 回日本微生物生態学会
  • Place of Presentation: 松山市
  • Year and Date: 2007-09-16
• [Presentation] Comparison of bacterial communities in fish farm sediments with different pollution levels (2007)
  • Author(s): N. Kawahara, K. Shigematsu, T. Miyadai and R. Kondo
  • Organizer: 第23回日本微生物生態学会
  • Place of Presentation: 松山市
  • Year and Date: 2007-09-16
• [Remarks] 近藤竜二.養殖場堆積物の微生物群集 平成20 年度日本水産学会水産増殖懇話会第2 回講演会(招待講演)
• [Remarks]
  • URL: http://www.s.fpu.ac.jp/webrykondo/kondo-J.html
• [Remarks]
  • URL: http://www.s.fpu.ac.jp/webrykondo/kondo-J.html