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Distribution and diversity of sulphate-reducing bacteria in marinesediments as determined using molecular marker

Research Project

Project/Area Number 19580215
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionFukui Prefectural University

Principal Investigator

KONDO Ryuji  Fukui Prefectural University, 生物資源学部, 准教授 (30244528)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords硫酸還元細菌 / 亜硫酸還元酵素遺伝子 / 養殖漁場堆積物 / 有機汚濁 / リアルタイムPCR / PCR-DGGE / 海底堆積物
Research Abstract

本研究では、機能遺伝子をマーカーとして利用し、日本ならびに韓国の沿岸海域の底泥中における硫酸還元細菌の分布生態と多様性を解析した。まず、亜硫酸還元酵素遺伝子を標的とした定量PCR 法による硫酸還元細菌の計数方法を開発した。この方法によって底泥中の硫酸還元細菌を計数したところ、有機物汚濁の進行に伴って硫酸還元細菌が増加することが明らかとなった。PCR-DGGE によってその群集組成を調べた結果、主要な硫酸還元細菌がDesulfobacteraceae 科およびDesulfobulbaceae 科であったが、水域によって硫酸還元細菌の組成が異なることが明らかとなった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (37 results)

All 2009 2008 2007 Other

All Journal Article (14 results) (of which Peer Reviewed: 8 results) Presentation (20 results) Remarks (3 results)

  • [Journal Article] Dominantbacterioplankton populations in themeromictic Lake Suigetsu as determined bydenaturing gradient gel electrophoresis of16S rRNA gene fragments2009

    • Author(s)
      R. Kondo, A. Nakagawa, L. Mochizuki, K.Osawa, Y. Fujioka and J. Butani
    • Journal Title

      Limnology 10(1)

      Pages: 63-69

    • Related Report
      2008 Final Research Report
  • [Journal Article] Comparison of bacterialcommunities in fish farm sediments along anorganic enrichment gradient2009

    • Author(s)
      N. Kawahara, K. Shigematsu, T. Miyadaiand R. Kondo
    • Journal Title

      Aquaculture 287 (1-2)

      Pages: 107-113

    • Related Report
      2008 Final Research Report
  • [Journal Article] Comparison of bacterial communities in fish farm sediments along an organic enrichment gradient2009

    • Author(s)
      N. Kawahara, K. Shigematsu, T. Miyadai, R. Kondo
    • Journal Title

      Aquaculture 287

      Pages: 107-113

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dominant bacterioplankton populations in the meromictic Lake Suigetsu as determined by denaturing gradient gel electrophoresis of 16S rRNA gene fragments2009

    • Author(s)
      R. Kondo, A. Nakagawa, L. Mochizuki, K. Osawa, Y. Fujioka, J. Butani
    • Journal Title

      Limnology 10

      Pages: 63-69

    • NAID

      10025405069

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid enumeration of sulphate-reducingbacteria from aquatic environments usingreal-time PCR2008

    • Author(s)
      R. Kondo, K. Shigematsu and J. Butani
    • Journal Title

      Plankton & BenthosResearch 3 (3)

      Pages: 180-183

    • Related Report
      2008 Final Research Report
  • [Journal Article] Distribution ofsulfate-reducing bacteria in fish firmsediments2008

    • Author(s)
      N. Kawahara, K. Shigematsu, S. Miura, T.Miyadai and R. Kondo
    • Journal Title

      Plankton & Benthos Research 3(1)

      Pages: 42-45

    • Related Report
      2008 Final Research Report
  • [Journal Article] Distribution of sulfate-reducing bacteria in fish firm sediments2008

    • Author(s)
      N. Kawahara, K. Shigematsu, S. Miura, T. Miyadai, R. Kondo
    • Journal Title

      Plankton & Benthos Research 3

      Pages: 42-45

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid enumeration of sulphate-reducing bacteria from aquatic environments using real-time PCR2008

    • Author(s)
      R. Kondo, K. Shigematsu, J. Butani
    • Journal Title

      Plankton & Benthos Research 3

      Pages: 180-183

    • NAID

      10021907601

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid enumeration of sulphate-reducing bacteria from aquatic environments usingreal-time PCR2008

    • Author(s)
      R. Kondo, K. Shigematsu and J. Butani
    • Journal Title

      Plankton & Benthos Research (掲載確定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of sulfate-reducing bacteria in fish firm sediments2008

    • Author(s)
      N. Kawahara, K. Shigematsu, S. Miura, T. Miyadai and R. Kondo
    • Journal Title

      Plankton & Benthos Research 3

      Pages: 42-45

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of thediversity of sulfate-reducing bacterialcommunities in the water column andsurface sediments of a Japanese meromicticlake2007

    • Author(s)
      R. Kondo and J. Butani
    • Journal Title

      Limnology 8 (2)

      Pages: 131-141

    • Related Report
      2008 Final Research Report
  • [Journal Article] Spatial dynamics ofsulphate-reducing bacterial compositions insediments along a salinity gradient in a UKestuary2007

    • Author(s)
      R. Kondo, K. J. Purdy, S. Q. Silva and D. B.Nedwell
    • Journal Title

      Microbes and Environments 22 (1)

      Pages: 11-19

    • Related Report
      2008 Final Research Report
  • [Journal Article] Comparison of the diversity of sulphate-reducing bacterial communities in the water column and surface sediments of a Japanese meromictic lake2007

    • Author(s)
      R. Kondo and J. Butani
    • Journal Title

      Limnology 8

      Pages: 131-141

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial dynamics of sulphate-reducing bacterial compositions in sediments along a salinity gradient in a UK estuary2007

    • Author(s)
      R. Kondo, K. J. Purdy, S. Q. Silva and D. B. Nedwell
    • Journal Title

      Microbes and Environments 22

      Pages: 11-19

    • NAID

      110006242027

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] T-RFLP 法による魚類養殖漁場底泥中の細菌群集組成の比較2009

    • Author(s)
      坂見知子、奥村 裕、村岡大祐、横山 寿、重松孝太朗、近藤竜二
    • Organizer
      平成21 年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-30
    • Related Report
      2008 Final Research Report
  • [Presentation] T-RFLP法による魚類養殖漁場底泥中の細菌群集組成の比較2009

    • Author(s)
      坂見知子, 奥村裕, 村岡大祐, 横山 寿, 重松幸太朗, 近藤竜二
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] 日本および韓国沿岸域底泥中の硫酸還元細菌の分布と群集組成2009

    • Author(s)
      重松孝太朗、近藤竜二、坂見知子、横山寿、小泉喜嗣、尹 良湖
    • Organizer
      平成21 年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] 日本および韓国沿岸域底泥中の硫酸還元細菌の分布と群集組成2009

    • Author(s)
      重松幸太朗. 近藤竜二, 坂見知子, 横山 寿, 小泉喜嗣, 尹 良湖
    • Organizer
      平成21年度日本水産学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 紅色硫黄酸化細菌に特異的なPCR プライマーの開発2008

    • Author(s)
      森 裕美、近藤竜二
    • Organizer
      2008年日本プランクトン学会
    • Place of Presentation
      熊本市
    • Year and Date
      2008-09-06
    • Related Report
      2008 Final Research Report
  • [Presentation] 紅色硫黄酸化細菌に特異的なPCRプライマーの開発2008

    • Author(s)
      森 裕美, 近藤竜二
    • Organizer
      2008年日本プランクトン学会・日本ベントス学会合同大会
    • Place of Presentation
      熊本市
    • Year and Date
      2008-09-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Specific detection ofpurple sulfur bacteria using PCR primersthat target dissimilatory sulfite reductasegene2008

    • Author(s)
      Y. Mori and R. Kondo
    • Organizer
      12th International Symposium onMicrobial Ecology
    • Place of Presentation
      Cairns, Australia
    • Year and Date
      2008-08-21
    • Related Report
      2008 Final Research Report
  • [Presentation] Comparison of bacterial communities in fish farm sediments along an organic enrichment gradient2008

    • Author(s)
      R. Kondo, N. Kawahara, K. Shigematsu, T. Miyadai
    • Organizer
      12th International Symposium on Microbial Ecology
    • Place of Presentation
      Cairns, Australia
    • Year and Date
      2008-08-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] Comparison of bacterialcommunities in fish farm sediments along anorganic enrichment gradient2008

    • Author(s)
      R. Kondo, N. Kawahara, K. Shigematsu andT. Miyadai
    • Organizer
      12thInternational Symposium on MicrobialEcology
    • Place of Presentation
      Cairns, Australia
    • Year and Date
      2008-08-19
    • Related Report
      2008 Final Research Report
  • [Presentation] Specific detection of purple sulfur bacteria using PCR primers that target dissimilatory sulfite reductase gene2008

    • Author(s)
      Y. Mori, R. Kondo
    • Organizer
      12th International Symposium on Microbial Ecology
    • Place of Presentation
      Cairns, Australia
    • Year and Date
      2008-08-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] リアルタイムPCR法による硫酸還元細菌の迅速定量2008

    • Author(s)
      近藤竜二、重松孝太朗、部谷巡貴
    • Organizer
      平成20 年度日本水産学会春季大会
    • Place of Presentation
      静岡市
    • Year and Date
      2008-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] リアルタイムPCR法による硫酸還元細菌の迅速定量2008

    • Author(s)
      近藤竜二, 重松幸太朗, 部谷巡貴
    • Organizer
      平成20年度日本水産学会春季大会
    • Place of Presentation
      静岡市
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] 光合成硫黄細菌における亜硫酸還元酵素遺伝子の塩基配列の解析2008

    • Author(s)
      森 裕美、近藤竜二
    • Organizer
      第24 回日本微生物生態学会
    • Place of Presentation
      札幌市
    • Related Report
      2008 Final Research Report
  • [Presentation] 沿岸域底泥中の硫酸還元細菌の分布と群集組成2008

    • Author(s)
      重松孝太朗、尹 良湖、近藤竜二
    • Organizer
      第24 回日本微生物生態学会
    • Place of Presentation
      札幌市
    • Related Report
      2008 Final Research Report
  • [Presentation] 沿岸域底泥中の硫酸還元細菌の分布と群集組成2008

    • Author(s)
      重松孝太朗, 尹良湖, 近藤竜二
    • Organizer
      第24回日本微生物生態学会
    • Place of Presentation
      札幌市
    • Related Report
      2008 Annual Research Report
  • [Presentation] 光合成硫黄細菌における亜硫酸還元酵素遺伝子の塩基配列の解析2008

    • Author(s)
      森 裕美, 近藤竜二
    • Organizer
      第24回日本微生物生態学会
    • Place of Presentation
      札幌市
    • Related Report
      2008 Annual Research Report
  • [Presentation] PCR法による海面養殖場底泥中の硫酸還元細菌の分布と多様性解析2007

    • Author(s)
      重松孝太朗、川原直城、近藤竜二
    • Organizer
      平成19 年度日本水産学会秋季大会
    • Place of Presentation
      函館市
    • Year and Date
      2007-09-26
    • Related Report
      2008 Final Research Report
  • [Presentation] PCR法による海面養殖場底泥中の硫酸還元細菌の分布と多様性解析2007

    • Author(s)
      重松孝太朗, 川原直城, 近藤竜二
    • Organizer
      平成19年度日本水産学会秋季大会
    • Place of Presentation
      函館市
    • Year and Date
      2007-09-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Comparison of bacterialcommunities in fish farm sediments withdifferent pollution levels2007

    • Author(s)
      N. Kawahara, K. Shigematsu, T. Miyadaiand R. Kondo
    • Organizer
      第23 回日本微生物生態学会
    • Place of Presentation
      松山市
    • Year and Date
      2007-09-16
    • Related Report
      2008 Final Research Report
  • [Presentation] Comparison of bacterial communities in fish farm sediments with different pollution levels2007

    • Author(s)
      N. Kawahara, K. Shigematsu, T. Miyadai and R. Kondo
    • Organizer
      第23回日本微生物生態学会
    • Place of Presentation
      松山市
    • Year and Date
      2007-09-16
    • Related Report
      2007 Annual Research Report
  • [Remarks] 近藤竜二.養殖場堆積物の微生物群集 平成20 年度日本水産学会水産増殖懇話会第2 回講演会(招待講演)

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.s.fpu.ac.jp/webrykondo/kondo-J.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.s.fpu.ac.jp/webrykondo/kondo-J.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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