Analysis of physiological functions of the retinoid X receptor in prosobranch gastropods

• Project/Area Number: 19580226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: HORIGUCHI Toshihiro National Institute for Environmental Studies, 環境リスク研究センター, 主席研究員 (30260186)
• Co-Investigator(Kenkyū-buntansha): SHIRAISHI Hiroaki 独立行政法人国立環境研究所, 環境リスク研究センター, センター長 (10124348) ; OTA Yasuhiko 国立大学法人鳥取大学, 農学部, 教授 (60069078) ; 西川 智浩 独立行政法人国立環境研究所, 環境リスク研究センター, 契約職員 (10391137)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 前鰓類 / インポセックス / レチノイドX受容体 / 雄性生殖器 / ペニス / 輸精管 / 分化 / 成長

Research Abstract

前鰓類におけるRXRの機能解析を雄性生殖器の分化・成長との関係に着目して実施した。フィールドでの定期採集によりRXR遺伝子発現と雄性生殖器の発達の季節変化を示し、室内実験によりTBT曝露に伴うペニス形成部位の形態変化とRXR遺伝子発現の経時変化を示した。免疫染色によりRXRが前鰓類に広く分布することを示した。筋肉注射試験の結果、9-cis レチノイン酸の効果にも種差は見られないと考えられた。生殖輸管の分化・形成機序を組織学的に解析した。

Research Products

• [Journal Article] Exposure to 9-cis retinoic acid induces penis and vas deferens development in the female rock shell, Thais clavigera (2008)
  • Author(s): Horiguchi, T., Ohta, Y., Nishikawa, T., Shiraishi, F., Shiraishi, H., Morita, M.
  • Journal Title: Cell Biol. Toxicol. 24 Pages: 553-562
  • Peer Reviewed
• [Journal Article] A Mu-class glutathione S-transferase (GSTM) from the rock shell Thais clavigera (2008)
  • Author(s): Rhee, J. S., Raisuddin, S., Hwang, D. S., Horiguchi, T., Cho, H. S., Lee, J. S.
  • Journal Title: Comp. Biochem. Physiol., Part C 148 Pages: 195-203
  • Peer Reviewed
• [Journal Article] 有機スズによる巻貝類のインポセックス誘導メカニズム レチノイドX受容体関与説の提唱 (2008)
  • Author(s): 堀口敏宏
  • Journal Title: 化学と生物 46 Pages: 370-373
  • NAID: 10024333584
• [Journal Article] Exposure to 9-cis retinoic acid induces penis and vas deferens development in the female rock shell,Thais clavigera (2008)
  • Author(s): Horiguchi, T., Ohta, Y., Nishikawa, T., Shiraishi, F., Shiraishi, H., Morita, M.
  • Journal Title: Cell Biology and Toxicology
  • Peer Reviewed
• [Journal Article] Retinoid X receptor gene expression and protein content in tissues of the rock shell Thais clavigera (2007)
  • Author(s): Horiguchi, T., Nishikawa, T., Ohta, Y., Shiraishi, H., Morita, M.
  • Journal Title: Aquatic Toxicology 84 Pages: 379-388
  • Peer Reviewed
• [Journal Article] Endocrine disrupting organotin compounds are potent jnducers of hnposex in gastropods and adipogenesis in vertebrates (2007)
  • Author(s): Iguchi, T., Katsu, Y., Horiguchi, T., Watanabe, H., Blumberg, B., Ohta, Y.
  • Journal Title: Molecular and Cell Toxicology 3 Pages: 1-10
  • Peer Reviewed
• [Journal Article] 腹足類の内分泌系とインポセックスの発症機構 (2007)
  • Author(s): 堀口 敏宏
  • Journal Title: Biomedical Research on Trace Elements 18 Pages: 231-240
  • NAID: 10019765946
  • Peer Reviewed
• [Presentation] Annual trend of imposex and organotins content in the Rock shell, Thais clavigera from the coast of Korea (2008)
  • Author(s): Cho, H.S., Park, J.C., Choi, M.K., Choi, H.G., Horiguchi, T.
  • Organizer: 環境ホルモン学会第11回研究発表会, 同講演要旨集, p.164
  • Place of Presentation: 東京
• [Presentation] 海産腹足類と有機スズ化合物:生殖生理と内分泌攪乱の作用機序 (2008)
  • Author(s): 堀口敏宏
  • Organizer: 日本比較免疫学会第20回学術集会講演要旨p.29
  • Place of Presentation: 東京
• [Presentation] Endocrine disruption caused by organotins in gastropods: from a molecular mechanism to population-level effects (2008)
  • Author(s): Horiguchi, T., Cho, H.S., Imai, T., Hamada, F., Ohta, Y., Iguchi, T., Morishita, F., Matsushima, O., Nagao, T., Nishikawa, J., Shiraishi, F., Shimizu, M., Yamazaki, S., Morita, M., Shiraishi, H.
  • Organizer: Physical and Chemical Impacts on Marine Organisms -Seeking Sustainability and Postgenomics- pp.13-14
  • Place of Presentation: Nagoya(Japan)
• [Presentation] Endocrine disruption caused by organotin compounds in gastropod molluscs: from a molecular mechanism to population-level effects (2008)
  • Author(s): Horiguchi, T.
  • Organizer: Gaps in Pollution Sciences, Abstract Book p.13
  • Place of Presentation: Copenhagen (Denmark)
• [Presentation] Endocrine disruption caused by organotin compounds in gastropod molluscs: from a molecular mechanism to population-level effects (2008)
  • Author(s): Horiguchi, T.
  • Organizer: 1st International Conference on Environmental Health Science, p.52
  • Place of Presentation: Seoul (Korea)
• [Presentation] Induction mechanism of imposex caused by organotin compounds in gastropods: evidence concludes involvement of the retinoid X receptor (2008)
  • Author(s): Horiguchi, T., Shiraishi, H., Ohta, Y., Shiraishi, F., Cho, H.S., Morita, M.
  • Organizer: 5th World Fisheries Congress, Program and Abstracts, p.442
  • Place of Presentation: Yokohama (Japan)
• [Presentation] Imposex and organotin concentrations in Thais orbita and Morula marginalba around Sydney, Australia (2008)
  • Author(s): Horiguchi, T., Colville, A., Hamilton, L., Shiraishi, F., Nakajima, D., Kamata, R., Lim, R., Shiraishi, H.
  • Organizer: 5th SETAC World Congress
  • Place of Presentation: Sydney (Australia)
• [Presentation] Localization of RXR in penis and other tissues of several gastropod species (Muricidae, Buccinidae and Nassariidae) from Japan and Portugal (2008)
  • Author(s): Horiguchi, T., Sousa, A., Ohta, Y., Cho, H.S., Miguez, C., Shiraishi, H.
  • Organizer: SETAC Europe 18th Annual Meeting, p.150
  • Place of Presentation: Warsaw (Poland)
• [Presentation] Monthly changes of RXR gene expression and sexual characteristics in male rock shells (Thais clavigera) in Hiraiso, Japan (2008)
  • Author(s): Horiguchi, T., Nishikawa, T., Ohta, Y., Shiraishi, H.
  • Organizer: SETAC Europe 18th Annual Meeting, p.150
  • Place of Presentation: Warsaw (Poland)
• [Presentation] Monthly changes of RXR gene expression and sexual characteristics in male rock shells (Thais clavigera) in Hiraiso, Japan (2008)
  • Author(s): Horiguchi, T., Nishikawa, T., Ohta, Y., Shiraishi, H.
  • Organizer: SETAC Europe 18th Annual Meeting
  • Place of Presentation: Warsaw (Poland)
• [Presentation] Localization of RXR in penis and other tissues of several gastropod species (Muricidae, Buccinidae and Nassariidae) from Japan and Portugal (2008)
  • Author(s): Horiguchi, T., Sousa, A., Ohta, Y., Cho, H. S., Miguez, C., Shirarshi, H.
  • Organizer: SETAC Europe 18th Annual Meeting
  • Place of Presentation: Warsaw (Poland)
• [Presentation] Induction mechanism of imposex caused by organotin compounds in gastropods : evidence concludes involvement of the retinoid X receptor (2008)
  • Author(s): Horiguchi, T., Shiraishi, H., Ohta, Y., Shiraishi, F., Cho, H. S., Morita, M.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Yokohama (Japan)
• [Presentation] Endocrine disruption caused by organotin compounds in gastropod molluscs : from a molecular mechanism to population-level effects (2008)
  • Author(s): Horiguchi, T.
  • Organizer: 1st International Conference on Environmental Health Science
  • Place of Presentation: Seoul (Korea)
• [Presentation] Endocrine disruption caused by organotin compounds in gastropod molluscs : from a molecular mechanism to population-level effects (2008)
  • Author(s): Horiguchi, T.
  • Organizer: Gaps in Pollution Sciences
  • Place of Presentation: Copenhagen (Denmark)
• [Presentation] Endocrine disruption caused by organotins in gastropods : from a molecular mechanism to population-level effects (2008)
  • Author(s): Horiguchi, T., Cho, H.S., Imai, T., Hamada, F., Ohta, Y., Iguchi, T., Morishita, F., Matsushima, O., Nagao, T., Nishikaw, J., Shiraishi, F., Shimizu, M., Yamazaki, S., Morita, M., Shiraishi, H.
  • Organizer: Physical and Chemical Impacts on Marine Organisms -Seeking Sustainability and Postgenomics-
  • Place of Presentation: Nagoya(Japan)
• [Presentation] 海産腹足類と有機スズ化合物:生殖生理と内分泌攪乱の作用機序 (2008)
  • Author(s): 堀口敏宏
  • Organizer: 日本比較免疫学会第20回学術集会
  • Place of Presentation: 東京
• [Presentation] Annual trend of imposex and organotins content in the Rock shell, Thais clavigera from the coast of Korea (2008)
  • Author(s): Cho, H. S., Park, J. C., Choi, M. K., Choi, H. G., Horiguchi, T.
  • Organizer: 環境ホルモン学会第11回研究発表会
  • Place of Presentation: 東京
• [Presentation] 本邦産及び欧州産腹足類(アクキガイ科、エゾバイ科及びオリイレヨフバイ科)数種に対するRXR抗体の免疫組織化学染色 (2007)
  • Author(s): 堀口敏宏・Sousa, A.・太田康彦・趙顯書・Barroso, C.M.・白石寛明
  • Organizer: 環境ホルモン学会第10回研究発表会, 同講演要旨集, p.129
  • Place of Presentation: 埼玉
• [Presentation] 茨城・平磯におけるイボニシのRXR遺伝子発現及び性成熟関連形質の経月変化 (2007)
  • Author(s): 堀口敏宏・西川智浩・太田康彦・白石寛明
  • Organizer: 環境ホルモン学会第10回研究発表会, 同講演要旨集, p.70
  • Place of Presentation: 埼玉
• [Presentation] Endocrine disruption caused by organotin compounds in gastropod molluscs: from a molecular mechanism to population-level effects (2007)
  • Author(s): Horiguchi, T.
  • Organizer: International Symposium on the Environmental Risks of Chemicals, p.32-33
  • Place of Presentation: Saitama (Japan)
• [Presentation] Involvement of the retinoid X receptor in the development of imposex caused by organotin compounds in female gastropods (2007)
  • Author(s): Horiguchi, T., Shiraishi, H., Nishikawa, T., Ohta, Y., Shiraishi, F., Cho, H., Nishikawa, J., Morita, M.
  • Organizer: DIOXIN 2007 International Symposium, p135
  • Place of Presentation: Tokyo
• [Presentation] Long term trends of imposex in the rock shell, Thais clavigera and organotin pollutions in the coast of Korea (2007)
  • Author(s): Cho, H.S., Lee, J.H., Cho, C.R., Choi, H.G., Choi, M.G., Horiguchi, T.
  • Organizer: SETAC (Society of Environmental Toxicology and Chemistry) Europe 17th Annual Meeting, p.224
  • Place of Presentation: Porto (Portugal)
• [Presentation] Involvement of the retinoid X receptor in the development of imposex caused by organotins in female gastropods (2007)
  • Author(s): Horiguchi, T., Shiraishi, H., Nishikawa, T., Ohta, Y., Shiraishi, F., Cho, H.S., Nishikawa, J., Morita, M.
  • Organizer: SETAC(Society of Environmental Toxicology and Chemistry) Europe 17th Annual Meeting, p.31
  • Place of Presentation: Porto (Portugal)
• [Presentation] Involvement of the retinoid X receptor in the development of imposex caused by organotin compounds in female gastropods (2007)
  • Author(s): Horiguchi, T., Shiraishi, H., Nishikawa, T., Ohta, Y., Shiraishi, F., Cho, H.S., Nishikawa, J., Morita, M.
  • Organizer: DIOXIN 20071nternational Symposium
  • Place of Presentation: Tokyo(Japan)
• [Presentation] Endocrine disruption caused by organothl compounds in gastmpod molluscs:from a molecular mechanism to population-level effects (2007)
  • Author(s): Horiguchi, T.
  • Organizer: International Symposium on the Environmental Risks ofChemicals
  • Place of Presentation: Saitama(Japan)
• [Presentation] 茨城・平磯におけるイボニシのRXR遺伝子発現及び性成熟関連形質の経月変化 (2007)
  • Author(s): 堀口 敏宏・西川 智浩・太田 康彦・白石 寛明
  • Organizer: 環境ホルモン学会第10回研究発表会
  • Place of Presentation: 埼玉
• [Presentation] 本邦産及び欧州産腹足類(アクキガイ科、エゾバイ科及びオリイレヨフバイ科)数種に対するRXR抗体の免疫組織化学染色 (2007)
  • Author(s): 堀口 敏宏・Sousa, A.・太田 康彦・趙 顯書・Barroso, C.M.・白石 寛明
  • Organizer: 環境ホルモン学会第10回研究発表会
  • Place of Presentation: 埼玉
• [Book] Chapter 7, Mechanism of imposex induced by organotins in gastropods. Ecotoxicology of Antifouling Biocides (Arai T et al. eds.) (2009)
  • Author(s): Horiguchi, T.
  • Publisher: Springer, Tokyo, Japan
• [Book] Chapter 8, The endocrine-disrupting effect of organotin compounds for aquatic organisms. Ecotoxicology of Antifouling Biocides (Arai T et al. eds.) (2009)
  • Author(s): Horiguchi, T.
  • Publisher: Springer, Tokyo, Japan
• [Book] Ecotoxicology of Antifouling Biocides (Arai T et al. eds.) : Chapter 7, Mechanism of imposex induced by organotins in gastropods., pp. 111-124 (2009)
  • Author(s): Horiguchi, T.
  • Total Pages: 437
  • Publisher: Springer, Tokyo, Japan
• [Book] Ecotoxicology of Antifouling Biocides (Arai T et al. eds.) : Chapter 8, The endocrine-disrupting effect of organotin compounds for aquatic organisms., pp. 125-146 (2009)
  • Author(s): Horiguchi, T.
  • Total Pages: 437
  • Publisher: Springer, Tokyo, Japan
• [Book] 2・2・1 巻貝のインポセックス発生状況から見た汚染実態の変遷およびアワビ類における有機スズの影響. 有機スズと環境科学-進展する研究の成果-(山田 久編) (2007)
  • Author(s): 堀口敏宏
  • Publisher: 恒星社厚生閣
• [Book] 3・1・3 巻貝類のインポセックス発症機構. 有機スズと環境科学-進展する研究の成果-(山田 久編) (2007)
  • Author(s): 堀口敏宏
  • Publisher: 恒星社厚生閣
• [Book] 有機スズと環境科学-進展する研究の成果 (2007)
  • Author(s): 堀口 敏宏, ほか
  • Total Pages: 314
  • Publisher: 恒星社厚生閣
• [Remarks]
  • URL: http://www.nies.go.jp/risk/index.html