Cloning and molecular evolutionary genetics analysis of paralytic shellfish toxin transforming enzyme from bivalves

• Project/Area Number: 19580231
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Tohoku University
• Principal Investigator: CHO Yuko Tohoku University, 大学院・農学研究科, 助教 (60323086)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 水産学 / 生理活性 / 変換酵素 / 二枚貝 / 麻痺性貝毒 / 生理活性物質

Research Abstract

麻痺性貝毒変換酵素は本研究代表者のグループが世界ではじめて2種の国産二枚貝から単離に成功した酵素である。本酵素の起源を解明するためサラガイとバカガイの麻痺性貝毒変換酵素性状を比較したところ、異なる分子種の酵素と推定された。さらに遺伝子のクローニングを試みサラガイのライブラリーからスルホカルバミラーゼIの遺伝子の候補と考えられる121bpの断片を得た。遺伝子の全長取得にむけて今後の研究が展開できる成果であった。

Research Products

• [Journal Article] 二枚貝の麻痺性貝毒変換酵素 低毒成分の毒性を100倍にする新規な酵素Sulfocalbamoylase Iの単離と性状 (2009)
  • Author(s): 長由扶子、小川徳之、大島泰克
  • Journal Title: 化学と生物 47 Pages: 80-82
• [Journal Article] 二枚貝の麻痺性貝毒変換酵素低毒成分の毒性を100倍にする新規な酵素Sulfocalbamoylase Iの単離と性状 (2009)
  • Author(s): 長由扶子、小川徳之、大島泰克
  • Journal Title: 化学と生物 47 Pages: 80-82
• [Journal Article] Purification and characterization of paralytic shellfish toxin transforming enzyme, sulfocarbamoylase I, from Japanese bivalve (2008)
  • Author(s): Yuko Cho, Noriyuki Ogawa, Miyako Takahashi, His-Pin Lin, Yasukatsu Oshima
  • Journal Title: Peronidia venulosa BBA - Proteins and Proteomics 1784 Pages: 1277-1285
  • Peer Reviewed
• [Journal Article] Purification and characterization of paralytic shellfish toxin transforming enzyme, sulfocarbamoylase I, from Japanese bivalve,Peronidia venulosa (2008)
  • Author(s): Yuko Cho, Noriyuki Ogawa, Miyako Takahashi, His-Pin Lin, Yasukatsu Oshima
  • Journal Title: BBA-Proteins and Proteomics 1784 Pages: 1277-1285
  • Peer Reviewed
• [Presentation] Sulfocarbamoylase I, a novel paralytic shellfish toxin transforming enzyme from bivalves (2008)
  • Author(s): Yuko Cho, Noriyuki Ogawa, Miyako Takahashi, His-Pin Lin and Yasukatsu Oshima
  • Organizer: Peronidia venulosa, The 13th international Conference on Harmful Algae
  • Place of Presentation: 香港、中国
• [Presentation] Sulfocarbamoylase I, a novel paralytic shellfish toxin transforming enzyme from bivalves, Peronidia venulosa (2008)
  • Author(s): Yuko Cho, Noriyuki Ogawa, Miyako Takahashi, His-Pin Lin and Yasukatsu Oshima
  • Organizer: The 13th international Conference on Harmful Algae
  • Place of Presentation: 香港中国
• [Presentation] Paralytic shellfish toxin transforming enzymes from bivalves, Peronidia venulosa (2007)
  • Author(s): Yuko Cho, Noriyuki Ogawa, Miyako Takahashi, His-Pin Lin and Yasukatsu Oshima
  • Organizer: ゴードン会議
  • Place of Presentation: アメリカ合衆国、、ウォータービル、コルビーカレッジ