Transfer and expression of germ cells between New and Old World quails

• Project/Area Number: 19580308
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Shinshu University
• Principal Investigator: ONO Tamao Shinshu University, 農学部, 教授 (10177264)
• Co-Investigator(Kenkyū-buntansha): ASANO Anshin 帝京大学, 医学部, 助教 (70459311)
• Co-Investigator(Renkei-kenkyūsha): KAGAMI Hiroshi 信州大学, 農学部, 教授 (80308303) ; SHIMADA Kiyoshi ソウル大学(韓国), 農学生命科学研究科, 教授 (40065579) ; TAGAMI Takahiro 独立行政法人農業・食品産業技術総合研究機構, 主任研究員 (60355104)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 家畜生産システム / 家禽繁殖 / ニホンウズラ / コリンウズラ / ニワトリ / ヒメウズラ / 新世界ウズラ / 旧世界ウズラ / PCR / 実験動物 / プライマー / 胚培養 / キメラ / 始原生殖細胞 / 特異抗体

Research Abstract

Basic biological information of the bobwhite quail belongs to New World quail was obtained. (1) Ex vivo culture system leading to hatching was devised. (2) PCR systems were put to practical use to identify its genome and sex. (3) New and Old world quails were different in chromosomal level. (4) Normal developmental tables were made. (5) Donor-derived DNA products were observed in chimeras between the bobwhite quail and Japanese quail embryos.

Research Products

• [Journal Article] Viability and functionality of primordial germ cells after freeze-thaw in chickens (2011)
  • Author(s): Nakamura Y, Usui F, Ono T, Kagami H, TagamiT
  • Journal Title: Journal of Poultry Science 48(1) Pages: 57-63
  • NAID: 10027817694
  • Peer Reviewed
• [Journal Article] Novel method of gene transfer in birds : Intracytoplasmic sperm injection (ICSI) for green fluorescent protein (GFP) expression in quail blastoderms. (2010)
  • Author(s): Mizushima S, Mizushima S, Takagi S, Ono T, Shimada K.
  • Journal Title: Biology of Reproduction 83(6) Pages: 965-969
  • Peer Reviewed
• [Journal Article] Efficient system for preservation and regeneration of genetic resources in chicken : concurrent storage of primordial germ cells and live animals from early embryos of a rare indigenous fowl (Gifujidori). (2010)
  • Author(s): Nakamura Y, Usui F, Ono T, Kagami H, Tagami T.
  • Journal Title: Reproduction, Fertility and Development 22(8) Pages: 1237-1246
  • Peer Reviewed
• [Journal Article] Germl ine replacement by transfer of primordial germ cells into partially sterilized embryos in the chicken. (2010)
  • Author(s): Nakamura Y, Usui F, Ono T, Kagami H, Tagami T
  • Journal Title: Biology of Reproduction 83(1) Pages: 130-137
  • Peer Reviewed
• [Journal Article] A novel concentrating system of chicken stem cells by bone marrow side population cells. (2010)
  • Author(s): Usui F, Ono T, Kagami H
  • Journal Title: Journal of Poultry Science 47(1) Pages: 53-56
  • NAID: 10026253103
  • Peer Reviewed
• [Journal Article] Phospholipase Cz mRNA expression and its potency during spermatogenesi s for activation of quail oocyte as a sperm factor. (2010)
  • Author(s): Mizushima S, Ono T, Shimada K.
  • Journal Title: Molecular Reproduction and Development 76(12) Pages: 1200-1207
  • Peer Reviewed
• [Journal Article] Germline replacement by transfer of primordial germ cells into partially sterilized embryos in the chicken (2010)
  • Author(s): Nakamura Y,Ono T,Kagami H, Tagami T, 他3名, 3番目
  • Journal Title: Biology of Reproduction Volume: 83 Pages: 130-137
  • Peer Reviewed
• [Journal Article] Efficient system for preservation and regeneration of genetic resources in chicken: concurrent storage of primordial germ cells and live animals from early embryos of a rare indigenous fowl(Gifujidori) (2010)
  • Author(s): Nakamura Y,Ono T, Kagami H, Tagami T, 他5名, 5番目
  • Journal Title: Reproduction, Fertility and Development Volume: 22 Pages: 1237-1246
  • Peer Reviewed
• [Journal Article] Novel method of gene transfer in birds: Intracytoplasmic sperm injection(ICSI)for green fluorescent protein(GFP)expression in quail blastoderms (2010)
  • Author(s): Mizushima, S., Ono, T.Shimada, K., 他6名, 3番目
  • Journal Title: Biology of Reproduction Volume: 83 Pages: 965-969
  • Peer Reviewed
• [Journal Article] Viability and functionality of primordial germ cells after freeze-thaw in chickens (2010)
  • Author(s): Nakamura Y, Ono T, Kagami H, Tagami T, 他6名, 6番目
  • Journal Title: Journal of Poultry Science Volume: 48 Pages: 57-63
  • NAID: 10027817694
  • Peer Reviewed
• [Journal Article] A novel concentrating system of chicken stem cells by bone marrow side population cells (2010)
  • Author(s): Usui F, Ono T, Kagami H., (他4名, 6番目)
  • Journal Title: J Poult Sci 47(1) Pages: 53-56
  • NAID: 10026253103
  • Peer Reviewed
• [Journal Article] Phospholipase Cz mRNA expression and its potency during spermatogenesis for activation of quail oocyte as a sperm factor (2010)
  • Author(s): Mizushima S, Ono T, Shimada K., (他4名, 3番目)
  • Journal Title: Mol Reprod Dev 76(12) Pages: 1200-1207
  • Peer Reviewed
• [Journal Article] Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica) (2010)
  • Author(s): Mizushima S, Ono T, Shimada K., (他4名, 3番目)
  • Journal Title: J Exp Zool A 307A(11) Pages: 647-653
  • Peer Reviewed
• [Journal Article] A novel concentrating system of chicken stem cells bybone marrow side population cells (2010)
  • Author(s): Usui, F., Ono, T., Kagami, H., (他4名, 6番目)
  • Journal Title: Journal of Poultry Science 47 Pages: 53-56
  • Peer Reviewed
• [Journal Article] Analysis of developmental changes in avian DNA methylation using a novel method for quantifying genome-wide DNA methylation. (2009)
  • Author(s): Usui F, Ono T, Kagami H.
  • Journal Title: Journal of Poultry Science 46(4) Pages: 286-290
  • NAID: 10025549381
  • Peer Reviewed
• [Journal Article] Novel system for degeneration of blood vessels by UV irradiation and subsequent regeneration using chick bone marrow cells. (2009)
  • Author(s): Usui F, Ono T, Kagami H
  • Journal Title: Cells Tissues Organs 188(5) Pages: 348-355
  • Peer Reviewed
• [Journal Article] Depletion of primordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs. (2009)
  • Author(s): Atsumi Y, Kagami H, Ono T
  • Journal Title: journal of Poultry Science 46(2) Pages: 136-143
  • NAID: 10024799460
  • Peer Reviewed
• [Journal Article] Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos. (2009)
  • Author(s): Nakamura Y, Ono T, Kagami H, Tagami T
  • Journal Title: Journal of Poultry Science 46(2) Pages: 127-135
  • NAID: 10024799424
  • Peer Reviewed
• [Journal Article] Complete regeneration of muscular dystrophy chickens by mating of male and female offspring derived from germline chimeras. (2009)
  • Author(s): Fujiwara A, Ono T Hiramatsu K, Kagami H
  • Journal Title: Journal of Poultry Science 46(2) Pages: 123-126
  • NAID: 10024799414
  • Peer Reviewed
• [Journal Article] Regeneration of muscular dystrophy chickens by transplantation of early blastodermal cells into recipient embryos. (2009)
  • Author(s): Fujiwara A, Ono T, Kagami H
  • Journal Title: Journal of Poultry Science 46(1) Pages: 46-51
  • NAID: 10024798984
  • Peer Reviewed
• [Journal Article] Analysis of developmental changes in avian DNA methylation using a novel method for quantifying genome-wide DNA methylation (2009)
  • Author(s): Usui F, Ono T, Kagami H., (他3名, 4番目)
  • Journal Title: J Poult Sci 46(4) Pages: 286-290
  • NAID: 10025549381
  • Peer Reviewed
• [Journal Article] Novel system for degeneration of blood vessels by UV irradiation and subsequent regeneration using chick bone marrow cells (2009)
  • Author(s): Usui F, Ono T, Kagami H., (他2名, 3番目)
  • Journal Title: Cells Tissues Organs 188(5) Pages: 348-355
  • Peer Reviewed
• [Journal Article] Depletion of primordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs (2009)
  • Author(s): Atsumi Y, Kagami H, Ono T., (他3名, 6番目)
  • Journal Title: J Poult Sci 46(2) Pages: 136-143
  • NAID: 10024799460
  • Peer Reviewed
• [Journal Article] Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos (2009)
  • Author(s): Nakamura Y, Ono T, Kagami H, Tagami T., (他6名, 3番目)
  • Journal Title: J Poult Sci 46(2) Pages: 127-135
  • NAID: 10024799424
  • Peer Reviewed
• [Journal Article] Complete regeneration of muscular dystrophy chickens by mating of male and female offspring derived from germline chimeras (2009)
  • Author(s): Fujiwara A, Ono T, Hiramatsu K, Kagami H.
  • Journal Title: J Poult Sci 46(2) Pages: 123-126
  • NAID: 10024799414
  • Peer Reviewed
• [Journal Article] Regeneration of muscular dystrophy chickens by transplantation of early blastodermal cells into recipient embryos (2009)
  • Author(s): Fujiwara A, Ono T, Kagami H.
  • Journal Title: J Poult Sci 46(1) Pages: 46-51
  • NAID: 10024798984
  • Peer Reviewed
• [Journal Article] Complete regeneration of muscular dystrophy chickens by mating of male and female offspring derived from germline chimeras (2009)
  • Author(s): Fujiwara, A., Ono, T., Kagami, H.
  • Journal Title: Journal of Poultry Science 46 Pages: 123-126
  • NAID: 10024799414
  • Peer Reviewed
• [Journal Article] Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ Cells in early chicken embryos (2009)
  • Author(s): Nakamura, Y., Ono, T., Kagami, H., Tagami, T., (他3名, 3番目)
  • Journal Title: Journal of Poultry Science 46 Pages: 127-135
  • NAID: 10024799424
  • Peer Reviewed
• [Journal Article] Depletion of primordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs (2009)
  • Author(s): Atsui, Y., Kagami, H., Ono, T., (他3名, 6番目)
  • Journal Title: Journal of Poultry Science 46 Pages: 136-143
  • NAID: 10024799460
  • Peer Reviewed
• [Journal Article] Novel system for degeneration of blood vessels by UV irradiation and subsequent regeneration using chick bone marrow cells (2009)
  • Author(s): Usui, F., Ono, T., Kagami, H., (他2名, 3番目)
  • Journal Title: Cells Tissues Organs 188 Pages: 348-355
  • Peer Reviewed
• [Journal Article] Analysis of developmental changes in avian DNA methylation using a novel method for quantifying genome-wide DNA methylation (2009)
  • Author(s): Usui, F., Ono, T., Kagami, H., (他3名, 4番目)
  • Journal Title: Journal of Poultry Science 46 Pages: 286-290
  • NAID: 10025549381
  • Peer Reviewed
• [Journal Article] Phospholipase Cz mRNA expression and its potency during spermatogenesis for activation of quail oocyte as a sperm factor (2009)
  • Author(s): Mizushima, S., Ono, T., Shimada, K., (他4名, 3番目)
  • Journal Title: Molecular Reproduction and Development 46 Pages: 1200-1207
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 臼井, 文武・小野, 珠乙・鏡味, 裕
  • Journal Title: タイトル名:ニワトリ初期胚におけるDNAメチル化状態の変化(書名:DNA多型VOL.17)(東洋書店) Pages: 216-218
• [Journal Article] Regeneration of muscular dystrophy chickens by transplantation of early blastodermal cells into recipient embryos (2009)
  • Author(s): Fujiwara, A.
  • Journal Title: Journal of Poultry Science 46 Pages: 46-51
  • Peer Reviewed
• [Journal Article] Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens. (2008)
  • Author(s): Nakamura Y, Ono T Kagami H, Tagami T
  • Journal Title: Reproduction, Fertility and Development 20(8) Pages: 900-907
  • Peer Reviewed
• [Journal Article] Restriction of germline proliferation by soft x-ray irradiation of chicken embryos and its application to chimera production. (2008)
  • Author(s): Atsumi Y, Tagami T, Kagami H, Ono T
  • Journal Title: Journal of Poultry Science 45(4) Pages: 292-297
  • NAID: 10024337045
  • Peer Reviewed
• [Journal Article] Developmental enhancement of intracytoplasmic sperm injection (ICSI)-generated quail embryos by phospholipase CζcRNA. (2008)
  • Author(s): Mizushima S, Ono T, Shimada K.
  • Journal Title: Journal of Poultry Science 45(2) Pages: 152-158
  • NAID: 10021220283
  • Peer Reviewed
• [Journal Article] Effects of aromatase inhibitor (fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and FRa in embryos and growth in posthatching quail. (2008)
  • Author(s): Koba N, Ono T, Shimada K.
  • Journal Title: Journal of Poultry Science 45(2) Pages: 116-124
  • Peer Reviewed
• [Journal Article] Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens (2008)
  • Author(s): Nakamura Y, Ono T, Kagami H, Tagami T., (他6名, 6番目)
  • Journal Title: Reprod, Fert Develop 20(8) Pages: 900-907
  • Peer Reviewed
• [Journal Article] Restriction of germline proliferation by soft x-ray irradiation of chicken embryos and its application to chimera production (2008)
  • Author(s): Atsumi Y, Tagami T, Kagami H, Ono T.
  • Journal Title: J Poult Sci 45(4) Pages: 292-297
  • NAID: 10024337045
  • Peer Reviewed
• [Journal Article] Developmental enhancement of intracytoplasmic sperm injection (ICSI)-generated quail embryos by phospholipase Cζ cRNA (2008)
  • Author(s): Mizushima S, Ono T, Shimada K., (他4名, 3番目)
  • Journal Title: J Poult Sci 45(2) Pages: 152-158
  • NAID: 10021220283
  • Peer Reviewed
• [Journal Article] Effects of aromatase inhibitor (fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and ERα in embryos and growth in posthatching quail (2008)
  • Author(s): Koba N, Ono T, Shimada, K., (他5名, 7番目)
  • Journal Title: J Poult Sci 45(2) Pages: 116-124
  • NAID: 10021220131
  • Peer Reviewed
• [Journal Article] Effects of aromatase inhibitor (fadrozole)-induced sex-reversal on gonadal differentiation and mRNA expression of P450arom, AMH and ER α in embryos and growth in posthatching quail (2008)
  • Author(s): Koba, N.
  • Journal Title: Journal of Poultry Science 45 Pages: 116-124
  • Peer Reviewed
• [Journal Article] Developmental enhancement of intracytoplasmic sperm injection (ICSI)-generated quail embryos by phospholipase Cζ cRNA (2008)
  • Author(s): Mizushima, S.
  • Journal Title: Journal of Poultry Science 45 Pages: 152-158
  • Peer Reviewed
• [Journal Article] Restriction of germline proliferation by soft x-ray irradiation of chicken embryos and its application to chimera production (2008)
  • Author(s): Atsurai, Y.
  • Journal Title: Journal of Poultry Science 45 Pages: 292-297
  • Peer Reviewed
• [Journal Article] Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens (2008)
  • Author(s): Nakamura, Y.
  • Journal Title: Reproduction, Fertility and Development 20 Pages: 900-907
  • Peer Reviewed
• [Journal Article] Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica). (2007)
  • Author(s): Mizushima S, Ono T, Shimada K.
  • Journal Title: journal of Experimental Zoology Part A : Ecological Genetics and Physiology 307A(11) Pages: 647-653
  • Peer Reviewed
• [Journal Article] Migration and proliferation of primordial germ cells in the early chicken embryo. (2007)
  • Author(s): MNakamura Y, Ono T, Kagami H, Tagami T
  • Journal Title: Poultry Science 86(10) Pages: 2182-2193
  • Peer Reviewed
• [Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome. (2007)
  • Author(s): Takagi S, Ono T, Shimada K.
  • Journal Title: Poultry Science 86(5) Pages: 937-943
  • Peer Reviewed
• [Journal Article] A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken. (2007)
  • Author(s): Yamamoto Y, Ono T, Kagami H
  • Journal Title: Biology of Reproduction 77(1) Pages: 115-119
  • Peer Reviewed
• [Journal Article] Z-chromosome specific primers for chicken-quail hybrid blastoderm. (2007)
  • Author(s): Takagi S, Ono T, Shimada K.
  • Journal Title: Journal of Poultry Science 44(2) Pages: 209-212
  • NAID: 130004446236
  • Peer Reviewed
• [Journal Article] Migration and proliferation of primordial germ cells in the early chicken embryo (2007)
  • Author(s): Nakamura Y, Ono T, Kagami H, Tagami T., (他6名, 5番目)
  • Journal Title: Poultry Sci 86(10) Pages: 2182-2193
  • Peer Reviewed
• [Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome (2007)
  • Author(s): Takagi S, Ono T, Shimada K, (他4名, 2番目)
  • Journal Title: Poultry Sci 86(5) Pages: 937-943
  • Peer Reviewed
• [Journal Article] A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken (2007)
  • Author(s): Yamamoto Y, Ono T, Kagami H., (他6名, 8番目)
  • Journal Title: Biol Reprod 77(1) Pages: 115-119
  • Peer Reviewed
• [Journal Article] Z-chromosome specific primers for chicken-quail hybrid blastoderm (2007)
  • Author(s): Takagi S, Ono T, Shimada, K., (他4名, 2番目)
  • Journal Title: J Poult Sci 44(2) Pages: 209-212
  • NAID: 130004446236
  • Peer Reviewed
• [Journal Article] Z-chromosome specific primers for chicken-quail hybrid blast oderm. (2007)
  • Author(s): Takagi, Soichi
  • Journal Title: Journal of Poultry Science 44 Pages: 209-212
  • Peer Reviewed
• [Journal Article] A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken. (2007)
  • Author(s): Yamamoto, Yasuhiro
  • Journal Title: Biology of Reproduction 77 Pages: 115-119
  • Peer Reviewed
• [Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome. (2007)
  • Author(s): Takagi, Soichi
  • Journal Title: Poultry Science 86 Pages: 937-943
  • Peer Reviewed
• [Journal Article] Migration and proliferation of primordial germ cells in the early chicken embryo. (2007)
  • Author(s): Nakamura, Yoshiaki
  • Journal Title: Poultry Science 86 Pages: 2183-2193
  • Peer Reviewed
• [Presentation] Effect of X-ray irradiation on primordial germ cell-depletion in chicken embryos. (2011)
  • Author(s): Nakamura Y, Ono T, Kagami H, Tagami T
  • Organizer: Fullpaper CD files of the 9th Asian Pacific Poultry Conference.Tue-S10-04,pp.1-4.
  • Place of Presentation: Taipei, Republic of China
  • Year and Date: 2011-03-22
• [Presentation] Effect of X-ray irradiation on primordial germ cell-depletion in chicken embryos (2011)
  • Author(s): Nakamura Y, Ono T, Kagami H, Tagami T, 他6名, 6番目
  • Organizer: 9th Asian Pacific Poultry Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-03-22
• [Presentation] Down-regulation of inositol 1,4,5-trisphosphate receptor type 1(IP3R-1)for oocyte activation in quail (2011)
  • Author(s): Mizushima S, Ono T, Shimada K, 他3名, 5番目
  • Organizer: 9th Asian Pacific Poultry Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-03-22
• [Presentation] Down-regulation of inositol 1,4,5-trisphosphate receptor type 1 (TP_3R-1) for oocyte activation in quail. (2011)
  • Author(s): Mizushima S, Ono T, Shimada K.
  • Organizer: Fullpaper CD files of the 9th Asian Pacific Poultry Conference.D-Tue-09, pp.1-4.
  • Place of Presentation: Taipei, Republic of China
  • Year and Date: 2011-03-21
• [Presentation] Down-regulation of inositol 1,4,5-trisphosphate receptor type 1 (IP_3R-1) for oocyte activation in quail. (2011)
  • Author(s): Shimada K, Mizushima S, Ono T, Kimura T
  • Organizer: Fullpaper CD files of the 9th Asian Pacific Poultry Conference.D-Tue-09, pp.1-4.
  • Place of Presentation: Taipei, Republic of China
  • Year and Date: 2011-03-21
• [Presentation] Establishment of intracytoplasmic sperm injection (ICSI)method for green fluorescent(GFP)expression in quail blastoderm (2011)
  • Author(s): Shimada K, Mizushima S, Ono T, Kimura I, 他6名, 4番目
  • Organizer: 9th Asian Pacific Poultry Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-03-21
• [Presentation] Basic information on blue-breasted quail and northern bobwhite quail for germline transfer studies. (2011)
  • Author(s): Ono T, Kagami H, Tsudzuki M, Matsuda Y
  • Organizer: 2nd International Workshop on Preservation of Avian Primordial Germ Cells and Its Usage
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2011-01-25
• [Presentation] Basic information on blue-breasted Qquail and northern bobwhite quail for germline transfer studies (2011)
  • Author(s): Ono T, Sakata A, Matsuda Y, 他5名, 1番目
  • Organizer: 2nd International Workshop on Preservation of Avian Primordial Germ Cells and Its Usage
  • Place of Presentation: Naha, Okinawa, Japan 招待講演
  • Year and Date: 2011-01-25
• [Presentation] 凍結融解操作がニワトリ始原生殖細胞の生存性ならびに機能性へ及ぼす影響 (2010)
  • Author(s): 中村隼明・小野珠乙, 他8名, 6番目
  • Organizer: 日本家禽学会秋季大会
  • Place of Presentation: 長野県南箕輪村
  • Year and Date: 2010-09-15
• [Presentation] 卵白を介したブスルファンの投与がニワトリ胚へ及ぼす影響 (2010)
  • Author(s): 森貴史・小野珠乙・鏡味, 他3名, 5番目
  • Organizer: 日本家禽学会秋季大会
  • Place of Presentation: 長野県南箕輪村
  • Year and Date: 2010-09-15
• [Presentation] c-myc遺伝子の導入を利用したニワトリ由来細胞株樹立の試み (2010)
  • Author(s): 宮原大地・小野珠乙・鏡味裕, 他5名, 7番目
  • Organizer: 日本家禽学会秋季大会
  • Place of Presentation: 長野県南箕輪村
  • Year and Date: 2010-09-15
• [Presentation] 幹細胞移植による筋ジストロフィー発症鶏の筋再生の解析 (2010)
  • Author(s): 渡辺晴陽・小野珠乙・鏡味裕, 他6名, 8番目
  • Organizer: 日本家禽学会秋季大会
  • Place of Presentation: 長野県南箕輪村
  • Year and Date: 2010-09-15
• [Presentation] Depletion of primordial germ cells for the better xenotransplanted germline donor in avian embryos. (2010)
  • Author(s): Ono T.
  • Organizer: GSR2010 Proceedings, WCU Biomodulation International Symposium on Germ Cell, tem Cell and Reproductive Biology
  • Place of Presentation: Je ju, Korea
  • Year and Date: 2010-06-07
• [Presentation] Intracytoplasmic sperm injection (ICSI) for avian transgenesis. (2010)
  • Author(s): Shimada K, Ono T, Mizushima S.
  • Organizer: GSR2010 Proceedings, WCU Biomodulation International Symposium on Germ Cell, Stem Cell and Reproductive Biology
  • Place of Presentation: Je ju, Korea
  • Year and Date: 2010-06-07
• [Presentation] Depletion of primordial germ cells for the better xenotransplanted germline donor in avian embryos (2010)
  • Author(s): Ono T
  • Organizer: GSR2010 Proceedings, WCU Biomodulation International Symposium on Germ Cell, Stem Cell and Reproductive Biology
  • Place of Presentation: Jeju, Korea 招待講演
  • Year and Date: 2010-06-07
• [Presentation] Intracytoplasmic sperm injection(ICSI) for avian transgenesis (2010)
  • Author(s): Shimada K, Ono T, Mizushima S
  • Organizer: GSR2010 Proceedings, WCU Biomodulation International Symposium on Germ Cell, Stem Cell and Reproductive Biology
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2010-06-07
• [Presentation] ニワトリ幹細胞・始原生殖細胞の遺伝的特徴解析と分化制御の試み (2010)
  • Author(s): 宮原大地・小野珠乙・鏡味裕, 他5名, 16番目
  • Organizer: 日本分子生物学会春季シンポジウム
  • Place of Presentation: 宮城県松島町
  • Year and Date: 2010-06-07
• [Presentation] ニワトリ始原生殖細胞の利用:移動様式の解明と凍結保存・移植・除去技術の確立 (2010)
  • Author(s): 中村隼明・小野珠乙・鏡味裕, 他7名, 6番目
  • Organizer: 日本分子生物学会春季シンポジウム
  • Place of Presentation: 宮城県松島町
  • Year and Date: 2010-06-07
• [Presentation] ニワトリ幹細胞・始原生殖細胞の培養と発生・分化における特性把握 (2010)
  • Author(s): 鏡味, 裕・小野, 珠乙, (他8名, 10番目)
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京都千代田区
  • Year and Date: 2010-03-29
• [Presentation] Establishment of intracytoplasmic sperm injection (ICSI) method for green fluorescent (GFP) expression in quail blastoderm. (2010)
  • Author(s): Mizushima S, Ono T, Shimada K.
  • Organizer: Fullpaper CD files of the 9th Asian Pacific Poultry Conference.Mon-S4-06,pp.1-4.
  • Place of Presentation: Taipei, Republic of China
  • Year and Date: 2010-03-22
• [Presentation] cDNA cloning, mRNA expression and oocyte-activating potential of quail phospholipase Czata. (2010)
  • Author(s): Mizushima S, Ono T, Shimada K.
  • Organizer: Proceedings of the 6th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology p. 80.
  • Place of Presentation: Palmerston North, New Zealand
  • Year and Date: 2010-01-21
• [Presentation] Roles of phospholipase C zeta, inositol trisphosphate and calcium in oocyte activation in quail. (2010)
  • Author(s): Shimada K Mizushima S, Ono T, Saito N.
  • Organizer: Proceedings of the 6th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology p.63.
  • Place of Presentation: Palmerston North, New Zealand
  • Year and Date: 2010-01-21
• [Presentation] Roles of phospholipase C zeta, inositol trisphosphate and calcium inoocyte activation in quail (2010)
  • Author(s): Shimada, K., Ono, T., (他6名, 5番目)
  • Organizer: 6th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology
  • Place of Presentation: Palmerston North, New Zealand
  • Year and Date: 2010-01-21
• [Presentation] cDNA cloning, mRNA expression and oocyte-activating potential of quail phospholipase Czata. (2010)
  • Author(s): Mizushima, S., Ono, T., (他3名, 4番目)
  • Organizer: 6th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology
  • Place of Presentation: Palmerston North, New Zealand
  • Year and Date: 2010-01-21
• [Presentation] 幹細胞・生殖細胞の遺伝的特徴把握と鳥類における発生・再生制御への活用の試み (2009)
  • Author(s): 鏡味, 裕・小野, 珠乙, (他8名, 10番目)
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-11
• [Presentation] ウズラ卵子活性化におけるIP3注入の効果 (2009)
  • Author(s): 水島, 秀成・小野, 珠乙, (他2名, 3番目)
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 沖縄県西原町
  • Year and Date: 2009-09-29
• [Presentation] 始原生殖細胞とその収集時に温存したニワトリ胚からの個体再生 (2009)
  • Author(s): 中村, 隼明・小野, 珠乙, (他7名, 5番目)
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 沖縄県西原町
  • Year and Date: 2009-09-28
• [Presentation] 胚盤葉明域中央部への局所的UV照射及び生殖新月環へのUV照射を用いたレシピエント胚由来PGCs数減少の試み (2009)
  • Author(s): 柏木, まや・小野, 珠乙, (他6名, 7番目)
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 沖縄県西原町
  • Year and Date: 2009-09-28
• [Presentation] ニワトリ幹細胞及び始原生殖細胞の発生に伴う遺伝子発現の解析と分化制御への活用の試み (2009)
  • Author(s): 鏡味, 裕・小野, 珠乙, (他8名, 10番目)
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 沖縄県西原町
  • Year and Date: 2009-09-28
• [Presentation] 凍結融解操作がニワトリ始原生殖細胞の生存性および生殖巣移住能へ及ぼす影響 (2009)
  • Author(s): 中村, 隼明・小野, 珠乙, (他7名, 5番目)
  • Organizer: 日本家禽学会秋季大会
  • Place of Presentation: 沖縄県西原町
  • Year and Date: 2009-09-27
• [Presentation] 造血幹細胞同定を目的とした致死量放射線量の探索 (2009)
  • Author(s): 臼井, 文武・小野, 珠乙, (他2名, 3番目)
  • Organizer: 日本家禽学会秋季大会
  • Place of Presentation: 沖縄県西原町
  • Year and Date: 2009-09-27
• [Presentation] 宿主胚の内因性資源生殖細胞の除去によるドナー由来の生殖系譜に置換されたキメラニワトリの作出 (2009)
  • Author(s): 中村, 隼明・小野, 珠乙, (他7名, 5番目)
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 奈良市
  • Year and Date: 2009-09-10
• [Presentation] Germline replacement by transferring primordial germ cells into sterilized embryos in the chicken (2009)
  • Author(s): Nakamura, Y., Ono, T., (他5名, 4番目)
  • Organizer: 2009 Poultry Science Association Annual Meeting
  • Place of Presentation: Raleigh, NC, USA
  • Year and Date: 2009-07-22
• [Presentation] Germline replacement by transferring primordial germ cells into sterilized embryos in the chicken (2009)
  • Author(s): Nakamura Y, Ono T, Kagami H, Tagami T
  • Organizer: 2009 Annual Meeting Abstracts
  • Place of Presentation: Poultry Science 88(suppl.1)
  • Year and Date: 2009-07-21
• [Presentation] ウズラ卵子DNase I活性に及ぼす精子数の効果 (2009)
  • Author(s): 杉本, 宰甫
  • Organizer: 日本家禽学会春季大会
  • Place of Presentation: 藤沢市
  • Year and Date: 2009-03-28
• [Presentation] 宿主に用いるニワトリ系統の違いが生殖系列キメリズムヘ及ぼす影響 (2009)
  • Author(s): 中村, 隼明
  • Organizer: 日本家禽学会春季大会
  • Place of Presentation: 藤沢市
  • Year and Date: 2009-03-28
• [Presentation] ニワトリ品種間における遺伝子配列差を利用したキメラの分子識別 (2009)
  • Author(s): 大友, 朝子
  • Organizer: 日本畜産学会第110回大会
  • Place of Presentation: 藤沢市
• [Presentation] 家禽幹細胞分化制御による新規育種戦略開拓の試み (2009)
  • Author(s): 鏡味, 裕
  • Organizer: 日本畜産学会第110回大会
  • Place of Presentation: 藤沢市
• [Presentation] Restriction of proliferation of primordial germ cells by soft X-ray irradiation in chick embryos. (2008)
  • Author(s): Atsumi Y, Tagami T, Kagami H, Ono T
  • Organizer: 23rd World Poultry Congress
  • Place of Presentation: Brisbane, Australia.
  • Year and Date: 2008-06-29
• [Presentation] Restriction of proliferation of primordial germ cells by soft X-ray irradiation in chick embryos (2008)
  • Author(s): Atsumi Y, Ono T., (他6名, 8番目)
  • Organizer: 23rd World Poultry Congress
  • Place of Presentation: Brisbane, Australia
  • Year and Date: 2008-06-29
• [Presentation] A novel strategy for preservation of genetic resources in birds. (2008)
  • Author(s): Nakamura Y, Ono T Kagami H, Tagami T
  • Organizer: Abstracts, Society for the Study of Reproduction 41st Annual Meeting and World Congress on Reproductive Biology, p.203.
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2008-05-28
• [Presentation] 新規PGCs単離法を用いた鳥類生殖系列キメラ個体と'ドナー由来後代の作出。 (2008)
  • Author(s): 柏木, まや
  • Organizer: 日本畜産学会
  • Place of Presentation: 日本畜産学会
  • Year and Date: 2008-03-29
• [Presentation] ブスルファン乳化液によるニワトリ始原生殖細胞の除去効果。 (2008)
  • Author(s): 中村, 隼明
  • Organizer: 日本家禽学会
  • Place of Presentation: 常磐大学
  • Year and Date: 2008-03-28
• [Presentation] 顕微授精ウズラ卵の発生能に及ぼすPhospholipase Czeta cRNA注入の効果。 (2008)
  • Author(s): 水島, 秀成
  • Organizer: 日本家禽学会
  • Place of Presentation: 常磐大学
  • Year and Date: 2008-03-28
• [Presentation] 各品種ニワトリのcPGCの培養 (2008)
  • Author(s): 大屋, 智子
  • Organizer: 第57回北信越畜産学会
  • Place of Presentation: 金沢市
• [Presentation] 培養アツセイによる造血幹細胞同定の試み (2008)
  • Author(s): 冨成, 和枝
  • Organizer: 第57回北信越畜産学会
  • Place of Presentation: 金沢市
• [Presentation] in vitroにおける鳥類始原生殖細胞の培養条件の探索 (2008)
  • Author(s): 大友, 朝子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大大会合同大会
  • Place of Presentation: 神戸市
• [Presentation] Whole Genomeにおける新規メチル化状態解析法の確立。 (2007)
  • Author(s): 臼井, 文武
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-28
• [Presentation] 幹細胞・始原生殖細胞の分化制御による鳥類発生工学の新戦略。 (2007)
  • Author(s): 鏡味, 裕
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-28
• [Presentation] ニワトリ胚盤葉細胞・始原生殖細胞の培養と特徴付け。 (2007)
  • Author(s): 伊東, 陽平
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-28
• [Presentation] 生殖系列キメラ作出のために改良した軟X線照射による効果的なPGCsの増殖抑制。 (2007)
  • Author(s): 渥美, 優介
  • Organizer: 鳥類内分泌研究会
  • Place of Presentation: 高松市 ニューグランデみまつ
  • Year and Date: 2007-11-16
• [Presentation] 烏骨鶏の黒色色素沈着を利用したキメラ判別の試み。 (2007)
  • Author(s): 行徳, 聡
  • Organizer: 鳥類内分泌研究会
  • Place of Presentation: 高松市 ニューグランデみまつ
  • Year and Date: 2007-11-16
• [Presentation] 精子抽出物および精子特異的PLCzeta cRNAの顕微注入に対するウズラ卵子の反応。 (2007)
  • Author(s): 水島, 秀成
  • Organizer: 鳥類内分泌研究会
  • Place of Presentation: 高松市 ニューグランデみまつ
  • Year and Date: 2007-11-16
• [Presentation] ニワトリ初期胚において生殖細胞を特徴づける遺伝子の発現解析。 (2007)
  • Author(s): 片倉, 真沙美
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 東京大学 農学部、弥生講堂
  • Year and Date: 2007-10-20
• [Presentation] 軟X線によるPGCsの増殖抑制とキメラの作出。 (2007)
  • Author(s): 渥美, 優介
  • Organizer: 日本家禽学会
  • Place of Presentation: 岡山大学島キャンパス
  • Year and Date: 2007-09-29
• [Presentation] ブスルファン乳化液の投与による血流中のニワトリ始原生殖細胞の除去効果の検証。 (2007)
  • Author(s): 中村, 隼明
  • Organizer: 日本家禽学会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2007-09-29
• [Presentation] アクチビンを用いたニワトリ未分化細胞の培養。 (2007)
  • Author(s): 河野, 聖
  • Organizer: 日本畜産学会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2007-09-27
• [Presentation] ニワトリ生殖細胞の新規単離法の開発と生殖細胞系列キメラニワトリの作出。 (2007)
  • Author(s): 山本, 耕裕
  • Organizer: 日本畜産学会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2007-09-26
• [Presentation] 発生工学による新規家禽育種戦略構築の試み。 (2007)
  • Author(s): 鏡味, 裕
  • Organizer: 日本畜産学会
  • Place of Presentation: 日本畜産学会
  • Year and Date: 2007-09-26
• [Presentation] Sexual difference in the number of primordial germ cells within the future gonadal region of chicken embryos. (2007)
  • Author(s): Nakamura, Yoshiaki
  • Organizer: 日本発生生物学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-05-28
• [Book] DNA多型VOL.17(ニワトリ初期胚におけるDNAメチル化状態の変化)(日本DNA多型学会編) (2009)
  • Author(s): 臼井文武・小野珠乙・鏡味裕
  • Publisher: 東洋書店
• [Book] ニワトリ初期胚におけるDNAメチル化状態の変化. DNA多型 VOL. 17 (日本DNA多型学会編) (2009)
  • Author(s): 臼井文武・小野珠乙・鏡味裕
  • Publisher: 東洋書店
• [Book] DNA多型VOL.15(DifferentialDisplayRT-PCRを用いたニワトリ初期胚での遺伝子発現様式)(日本DNA多型学会編) (2007)
  • Author(s): 山本耕裕・福田靖・小野珠乙・鏡味靖
  • Publisher: 東洋書店
• [Book] Differential Display RT-PCRを用いたニワトリ初期胚での遺伝子発現様式。(DNA多型 VOL. 15の分担執筆) (2007)
  • Author(s): 山本耕裕
  • Total Pages: 3
  • Publisher: 東洋書店
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