| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
The possible role of PI3-K in the reversible temperature-dependent immobilization of fowl sperm motility was investigated by using PI3 kinase inhibitor. Intact fowl sperm motility in TES/NaCl buffer remained negligible at the avian body temperature of 40℃ but was activated immediately after the addition of Ca^<2+>. The subsequent addition of inhibitor inhibited the motility again. In contrast, these inhibition was not observed in the demembranated spermatozoa at 40℃. These results suggest that PI3 kinase which is not retained in the axoneme and/or accessory cytoskeletal components, but in the cell membrane or cytoplasm, may be involved in the maintenance of flagellar movement of fowl spermatozoa at 40℃.
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