| Project/Area Number |
19580343
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Kagoshima University |
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Principal Investigator |
SUGIMOTO Yasushi Kagoshima University, 大学院・連合農学研究科, 教授 (10100736)
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| Co-Investigator(Kenkyū-buntansha) |
OKA Tatsuzou 鹿児島大学, 農学部, 教授 (50116795)
KUSAKABE Takahiro 九州大学, (連合)農学研究科(研究院), 准教授 (30253595)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | オボアルブミン / セルピン / 形態形成因子 / 神経管閉鎖障害 / 遺伝子発現 / 神経管閉鎖阻害 / NTD胚 / 発生異常 / 神経管欠損 / 熱安定型オボアルブミン / 形態形成 / 生理機能 |
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| Research Abstract |
Ovalbumin has been classified as a homolog of serine protease inhibitor (serpin), although little is known of its function except that it may be a nutrient source. We have shown that, during embryonic development, native molecules of ovalbumin (N-ovalbumin) are incorporated into tissues without degradation while undergoing configurational changes into a heat-stable form called 'HS-ovalbumin.' Here, to investigate the indispensability of HS-ovalbumin, we utilized an in vitro culture method by which chick embryos isolated from eggs at Stage 7 (before the neural tube begins to close) could develop normally for up to 24h. When endogenous, remnant ovalbumin was blocked by adding anti-ovalbumin polyclonal IgG to the medium, the isolated embryos became irregular particularly in brain tissue, resulting in the so-called neural tube deficiency (NTD). The embryos with NTD showed reduced signals for apoptosis and cell proliferation. Moreover, IgG-treated embryos were rescued by adding a sufficient
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amount of HS-ovalbumin to the medium, attaining normal morphologies without NTD. HS-ovalbumin exhibited a higher rescue rate than N-ovalbumin and S-ovalbumin, the latter being another heat-stable counterpart that occurs artificially by heating N-ovalbumin in vitro. These results indicate that ovalbumin (particularly in the HS-form) is essential in embryonic organogenesis, including neural tube formation. present results show also that the ovalbumin-deprived embryos gave decreased in situ signals for TUNEL reagents and for PCNA/c-Myc proteins, which mirror changes in apoptosis and cell proliferation, respectively. Also the ovalbumin deprival affect the embryos on many gene expressions including differentiation-inducing factors related to morphogenesis. Expressions of FGF8 and slug have decreased markedly, whereas those of BMP4 and Pax3 have increased over 3-fold. However, whether each of these alterations is a cause or a result of morphogenetic aberrancy is unclear. Since ovalbumin belongs to the serpin superfamily, there is a possibility that it shows direct interactions with some crucial differentiation-inducing factors. Less
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