The functional analysis of microtubule-associated protein, Spag5, and the rescue experiment of hypogonadic phenotype by gene transfer of wild type Spag5 cDNA.
| Project/Area Number |
19580350
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
SUZUKI Hiroetsu Nippon Veterinary and Life Science University, 獣医学部, 准教授 (50277662)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 遺伝 / 不妊 / 精巣 / 細胞分裂 / Spag5 / Astrin / 精巣形成不全 / アポトーシス / 腎低形成 / 細胞増殖 / astrin |
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| Research Abstract |
Microtubule-associated protein Spag5 is believed to be required for the progression of mitotic metaphase. However, it has been reported that Spag5-knockout mice had normal phenotype. To reveal in vivo-function of Spag5, we made Spag5-transgenic rats, and mated them to the rats from hypogonadic (hgn) strain. The hgn/hgn phenotype is associated with spontaneous mutation of Spag5. Male hgn/hgn is infertile, and abnormal mitosis is found in Sertoli cells of their testes. The hgn/hgn males with Spag5 transgene showed normal testis and fertility. Therefore, Spag5 is required for normal testicular development.
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Report
(4 results)
Research Products
(42 results)
