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Analysis of regulation on feline infectious peritonitis virus replication by RNA interference

Research Project

Project/Area Number 19580351
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

TANAKA Yoshikazu  Nippon Veterinary and Life Science University, 獣医学部, 講師 (50291159)

Co-Investigator(Kenkyū-buntansha) 盆子原 誠  日本獣医生命科学大学, 獣医学部, 講師 (50343611)
Co-Investigator(Renkei-kenkyūsha) BONKOBARA Makoto  日本獣医生命科学大学, 獣医学部, 講師 (50343611)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsウイルス / 感染症 / ネコ / ncRNA / 遺伝子制御 / ノンコーディングRNA / ウイルス複製制御
Research Abstract

fcwf-4細胞のFIPV感染実験においてウイルス複製に影響を与えるncRNAの分離・同定を試みた. マイクロDNA アレイによるmiRNA の解析を行い, hsa-miR-494 がウイルス感染時に2.1倍上昇することが明らかとなったが, hsa-miR-593, hsa-miR-193a-3p, hsa-miR-17 はそれぞれ0.11倍, 0.34倍, 0.44 倍と発現が減少した. 現在, 生理学的機能, 解析中である.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (23 results)

All 2008 2007

All Journal Article (12 results) (of which Peer Reviewed: 5 results) Presentation (6 results) Patent(Industrial Property Rights) (5 results)

  • [Journal Article] Changes in metabolite, energy metabolism related enzyme activities and peripheral blood mononuclear cell (PBMC) populations in beef heifers with two differing liveweight change profiles in New Zealand2008

    • Author(s)
      Mori A, Kenyon PR, Mori N, Yamamoto I, Tanaka Y, Suzuki N, Tazaki H, Ozawa T, Hayashi T, Hickson RE, Morris ST, Blair H, Arai T.
    • Journal Title

      Vet Res Commun(Epub 2007 Sep 12. PubMed PMID : 17849230) 32(2)

      Pages: 159-66

    • Related Report
      2008 Final Research Report
  • [Journal Article] Plasma metabolite concentrations and hepatic enzyme activities in pregnant Romney ewes with restricted feeding2008

    • Author(s)
      Tanaka Y, Mori A, Tazaki H, Imai S, Shiina J, Kusaba A, Ozawa T, Yoshida T, Kimura N, Hayashi T, Kenyon PR, Blair H, Arai T.
    • Journal Title

      Res Vet Sci(Epub 2007 Oct 24. PubMed PMID : 17920647) 85(1)

      Pages: 17-21

    • Related Report
      2008 Final Research Report
  • [Journal Article] Plasma metabolite concentrations and hepatic enzyme activities in pregnant Romney ewes with restricted feeding2008

    • Author(s)
      Tanaka Y.
    • Journal Title

      Res Vet Sci. 85(1)

      Pages: 17-21

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in metabolite, energy metabolism related enzyme activities and peripheral blood mononuclear cell (PBMC)populations in beef heifers with two differing liveweight change profiles in New Zealand.2008

    • Author(s)
      Mori A.
    • Journal Title

      Vet Res Commun. 32(2)

      Pages: 159-66

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma metabolite concentrations and hepatic enzyme activities in pregnant Romne yewes with restricted feeding.2008

    • Author(s)
      Tanaka Y
    • Journal Title

      Res Vet Sci. 5

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in metabolite, energy metabolism related enzyme activities and peripheral blood mononuclear cell(PBMC)populations in beef heifers with two differing liveweight change profiles in New Zealand.2008

    • Author(s)
      Mori A
    • Journal Title

      Vet Res Commun. 32(2)

      Pages: 159-66

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of plasma metabolite concentrations and enzyme activities in beef cattle raised by different feeding systems in Korea, Japan and New Zealand2007

    • Author(s)
      Mori A, Urabe S, Asada M, Tanaka Y, Tazaki H, Yamamoto I, Kimura N, Ozawa T, Morris ST, Hickson R, Kenyon PR, Blair H, Choi CB, Arai T.
    • Journal Title

      J Vet Med A Physiol Pathol Clin Med(PubMed PMID : 17718805) 54(7)

      Pages: 342-5

    • Related Report
      2008 Final Research Report
  • [Journal Article] Light-chain multiple myeloma in a cat2007

    • Author(s)
      Yamada O, Tamura K, Yagihara H, Isotani M, Azakami M, Sawada S, Ono K, Washizu T, Bonkobara M.
    • Journal Title

      J Vet Diagn Invest(PubMed PMID : 17609361) 19(4)

      Pages: 443-7

    • Related Report
      2008 Final Research Report
  • [Journal Article] Comparison of plasma metabolite concentrations and peripheral leukocyte enzyme activities in sheep fed on different diets in New Zealand and Japan2007

    • Author(s)
      Arai T, Kusaba A, Takeguchi A, Tanaka Y, Ozawa T, Yoshida T, Sako T, Hayashi T, Blair H.
    • Journal Title

      Vet Res Commun(Epub 2007 Jan 24. PubMed PMID : 17252317) 31(6)

      Pages: 681-4

    • Related Report
      2008 Final Research Report
  • [Journal Article] Reclassification of phenotypically identified staphylococcus intermedius strains2007

    • Author(s)
      Sasaki T, Kikuchi K, Tanaka Y, Takahashi N, Kamata S, Hiramatsu K.
    • Journal Title

      J Clin Microbiol(Epub 2007 Jun 27. PubMed PMID : 17596353 ; PubMed Central PMCID : PMC2045239) 45(9)

      Pages: 2770-8

    • Related Report
      2008 Final Research Report
  • [Journal Article] Methicillin-resistant Staphylococcus pseudintermedius in a veterinary teaching hospital2007

    • Author(s)
      Sasaki T, Kikuchi K, Tanaka Y, Takahashi N, Kamata S, Hiramatsu K.
    • Journal Title

      J Clin Microbiol(Epub 2007 Jan 31. PubMed PMID : 17267624 ; PubMed Central PMCID : PMC1865850) 45(4)

      Pages: 1118-25

    • Related Report
      2008 Final Research Report
  • [Journal Article] Comparison of plasma metabolite concentrations and enzyme activities in beef cattle raised by different feeding systems in Korea, Japan and New Zealand.2007

    • Author(s)
      Mori A
    • Journal Title

      J Vet Med A Physiol Pathol Clin Med. 54

      Pages: 342-5

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] RNAi法による猫伝染性腹膜炎ウイルスM遺伝子の発現抑制2007

    • Author(s)
      杉林 加奈
    • Organizer
      第144回 日本獣医学会学術集会
    • Place of Presentation
      北海道・酪農学園大学
    • Year and Date
      2007-09-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] RNAi法によるネコ伝染性腹膜炎ウイルス3CLpro蛋白質の発現抑制2007

    • Author(s)
      小澤 世里香
    • Organizer
      第144回 日本獣医学会学術集会
    • Place of Presentation
      北海道・酪農学園大学
    • Year and Date
      2007-09-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] リアルタイムPCR法を用いた猫伝染性腹膜炎の診断2007

    • Author(s)
      谷戸 三紗
    • Organizer
      第144回 日本獣医学会学術集会
    • Place of Presentation
      北海道・酪農学園大学
    • Year and Date
      2007-09-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] RNAi法によるネコ伝染性腹膜炎ウイルスM遺伝子の発現抑制2007

    • Author(s)
      杉林加奈(代表)
    • Organizer
      第144回日本獣医学会学術集会
    • Place of Presentation
      北海道酪農学園大学
    • Related Report
      2008 Final Research Report
  • [Presentation] RNAi法によるネコ伝染性腹膜炎ウイルス3C Lpro 蛋白質の発現抑制2007

    • Author(s)
      小澤世里香(代表)
    • Organizer
      第144回日本獣医学会学術集会
    • Place of Presentation
      北海道酪農学園大学
    • Related Report
      2008 Final Research Report
  • [Presentation] リアルタイムPCR法を用いた猫伝染性腹膜炎の診断2007

    • Author(s)
      谷戸三紗(代表)
    • Organizer
      第144回日本獣医学会学術集会
    • Place of Presentation
      北海道酪農学園大学
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] ネコ伝染性腹膜炎ウイルス(FIP)の予防および治療剤2008

    • Inventor(s)
      田中良和
    • Industrial Property Rights Holder
      学校法人日本医科大学
    • Filing Date
      2008-01-08
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] ネコ伝染性腹膜炎ウイルス(FIP)の予防および治療剤2008

    • Inventor(s)
      田中良和
    • Industrial Property Rights Holder
      学校法人日本医科大学
    • Filing Date
      2008-01-08
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] ネコFIPの予防及び治療剤2008

    • Inventor(s)
      田中良和
    • Industrial Property Rights Holder
      学校法人日本医科大学
    • Filing Date
      2008-01-08
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] ネコFIPの予防及び治療剤2008

    • Inventor(s)
      田中良和
    • Industrial Property Rights Holder
      学校法人日本医科大学
    • Filing Date
      2008-01-08
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] ネコ免疫不全ウイルス(FIV)の予防および治療剤2007

    • Inventor(s)
      田中良和
    • Industrial Property Rights Holder
      学校法人日本医科大学
    • Filing Date
      2007-10-18
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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