| Project/Area Number |
19580352
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Azabu University |
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Principal Investigator |
IKEDA Teruo Azabu University, 獣医学部, 准教授 (60151297)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Masaru 麻布大学, 獣医学部, 教授 (80271360)
SHIROTA Kinji 麻布大学, 獣医学部, 教授 (70147974)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | マスト細胞 / 細胞増殖 / 自然免疫 / IL-9 / シグナル伝達経路 / オートクライン / マスト細胞増殖因子 / SCF / LPS / IL-3 / マクロファージ / リアルタイム PCR / リアルタイムPCR |
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| Research Abstract |
The functions of mast cell in allergy responses have been extensively investigated. However, according to the detection of new mast cells functions, mast cell has been recognized as key players in the regulation of innate as well as adaptive immunity. We investigated the role of IL-9 related to the regulation of proliferation in lipopolysaccharide (LPS)-stimulated mast cells. The effect of IL-9 for mast cell proliferation was found only with SCF. These data suggest that number of mast cell increased by autocrine of IL-9, because mast cells constitutively express IL-9R. On the other hand IL-9 production in LPS-stimulated mast cells enhanced with IL-3. Transduction of dominant-negative (DN) form of MyD88, but not that of TLR-2 reduced IL-9 reporter activity. Moreover the treatment with the p38 inhibitor SB203580 and ERK inhibitor PD98059 supressed IL-9 secretion, indicating the IL-9 induction through p38 and ERK signaling pathway. These data indicate that IL-9 is an essential positive regulator of LPS-induced mast cell autocrine proliferation accompany with IL-3 and SCF and, therefore, is crucial for the host innate immune-responses to microbial products.
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