Diagnosis for Echinococcus definitive hosts in the early phase of infection and reconsideration of criteria for canine infection

• Project/Area Number: 19580353
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: University of Miyazaki (2008); Hokkaido University (2007)
• Principal Investigator: NONAKA Nariaki University of Miyazaki, 農学部獣医学科, 准教授 (50281853)
• Co-Investigator(Kenkyū-buntansha): KATAKURA Ken 北海道大学, 大学院・獣医学研究科, 教授 (10130155) ; OKU Yuzaburo 北海道大学, 大学院・獣医学研究科, 准教授 (60133716)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 人畜共通感染症 / エキノコックス / 犬 / PCR / 診断 / 多包条虫 / 終宿主 / DNA / COI

Research Abstract

エキノコックス終宿主動物の感染早期(感染後約4週間の虫卵排出前期)において宿主糞便中に排出されるエキノコックスDNAのPCR検出法を開発した。高感度プライマーの設計, PCR粗阻害物質除去などDNA抽出法や反応条散発的であったが, 宿主を駆虫して得られる駆虫後糞便を検査することによりDNA検出率件を最適化し, その信頼性を検証したところ, 感染早期ではDNA検出率は26~73%とは100%となった。

Research Products

• [Journal Article] A vague understanding of the biology and epidemiology of echinococcosis by dog owners in Hokkaido, an endemic island for Echinococcu smultilocularis in Japan (2009)
  • Author(s): Nonaka, N., Kamiya, M. and Oku, Y.
  • Journal Title: Journal of Veterinary Medical Science 71(1) Pages: 105-107
  • Peer Reviewed
• [Journal Article] Echinococcus multilocularis infection in pet dogs in Japan (2009)
  • Author(s): Nonaka, N., Kamiya, M., Kobayashi, F., Ganzorig, S., Ando, S., Yagi, K., Iwaki, T., Inoue, T. and Oku, Y.
  • Journal Title: Vector-borne and Zoonotic Diseases 9(2) Pages: 201-205
  • Peer Reviewed
• [Journal Article] Monitoring of environmental contamination by Echinococcus multilocularis in an urban fringe forest part in Hokkaido, Japan (2009)
  • Author(s): Lagapa, J. T., Oku, Y., Kaneko, M., Ganzorig, S., Ono, T., Nonaka, N.,Kobayashi, F. and Kamiya, M.
  • Journal Title: Environmental Health and Preventive Medicine 118(4) Pages: 576-582
  • Peer Reviewed
• [Journal Article] A vague understanding of the biology and epidemiology of echinococcosis by dog owners in Hokkaido, an endemic island for Echinococcus multilocularis in Japan (2009)
  • Author(s): Nonaka N.
  • Journal Title: Journal of Veterinary Medical Science 71 Pages: 105-107
  • NAID: 110007055397
  • Peer Reviewed
• [Journal Article] Echinococcus multilocularis infection in pet dogs in Japan (2009)
  • Author(s): Nonaka N.
  • Journal Title: Vector-borne and Zoonotic Diseases 9 Pages: 201-205
  • Peer Reviewed
• [Journal Article] On a comprehensive full-length cDNA library of larval Echinococcus multilocularis (2008)
  • Author(s): Oku, Y., Nonaka, N., Matsumoto, J., Arumua, M. T., Sugimoto, C., Watanabe, J., Toyoda, A. and Kamiya, M.
  • Journal Title: Japanese Journal of Veterinary Parasitology 6(2) Pages: 80-80
• [Journal Article] A first record of a cat excreting Echinococcus multilocularis eggs in Japan (2008)
  • Author(s): Nonaka, N., Hirokawa, H., Inoue, T.,Nakao, R., Ganzorig, S., Kobayashi, F.,Kamiya, M. and Oku, Y.
  • Journal Title: Japanese Journal of Veterinary Parasitology 6(2) Pages: 79-79
• [Journal Article] Molecular mechanisms involved in the growth and development of Echinococcus multilocularis metacestodes (2008)
  • Author(s): Matsumoto, J., Nonaka, N., Katakura, K.and Oku, Y.
  • Journal Title: Japanese Journal of Veterinary Parasitology 6(1) Pages: 7-12
• [Journal Article] Anaerobic NADH-fumarate reductase system is predominant in the respiratory chain of Echinococcus multilocularis, providing a novel target for the chemotherapy of alveolar echinococcosis (2008)
  • Author(s): Matsumoto, J., Sakamoto, K., Shinjyo,N., Kido, Y., Yamamoto, N., Yagi, K.,Miyoshi, H., Nonaka, N., Katakura, K., Kita, K. and Oku, Y.
  • Journal Title: Antimicrobial Agents and Chemotherapy 52(1) Pages: 164-170
  • Peer Reviewed
• [Journal Article] The first instance of a cat excreting Echinococcus multilocularis eggs in Japan (2008)
  • Author(s): Nonaka, N., Hirokawa, H., Inoue, T.,Nakao, R., Ganzorig, S., Kobayashi, F., Inagaki, M., Egoshi, K., Kamiya, M. and Oku, Y.
  • Journal Title: Parasitology International 57(4) Pages: 519-520
  • NAID: 10025329678
  • Peer Reviewed
• [Journal Article] A latex agglutination test for the detection of Echinococcus multilocularis coproantigen in the definitive hosts (2008)
  • Author(s): Nonaka, N., Oka, M., Kamiya, M. and Oku, Y.
  • Journal Title: Veterinary Parasitology 152(3-4) Pages: 278-283
  • Peer Reviewed
• [Journal Article] The first instance of a cat excreting Echinococcus multilocularis eggs in Japan (2008)
  • Author(s): Nonaka, N.
  • Journal Title: Japanese Journal of Veterinary Parasitology 57 Pages: 519-520
  • NAID: 10025329678
  • Peer Reviewed
• [Journal Article] Anaerobic NADH-fumarate reductase system is predominant in the respiratory chain of Echinococcus multilocularis, providing a novel target for the chemotherapy of alveolar echinococcosis (2008)
  • Author(s): Matsumoto, J.
  • Journal Title: Antimicrobial Agents and Chemotherapy 52 Pages: 164-170
  • Peer Reviewed
• [Journal Article] Molecular mechanisms involved in the growth and development of Echinococcus multilocularis metacestodes (2008)
  • Author(s): Matsumoto, J.
  • Journal Title: Japanese Journal of Veterinary Parasitology 6 Pages: 7-12
• [Journal Article] A first record of a cat excreting Echinococcus multilocularis eggs in Japan (2008)
  • Author(s): Nonaka, N.
  • Journal Title: Japanese Journal of Veterinary Parasitology 6 Pages: 79-79
• [Journal Article] On a comprehensive full-length cDNA library of larval Echinococcus multilocularis (2008)
  • Author(s): Oku, Y.
  • Journal Title: Japanese Journal of Veterinary Parasitology 6 Pages: 80-80
• [Journal Article] Taenia taeniaeformis : Fate and proliferation of mucosal cells during gastric hyperplasia in larvae infected rats (2008)
  • Author(s): Lagapa, J. T.
  • Journal Title: Experimental Parasitology 118 Pages: 576-582
  • Peer Reviewed
• [Journal Article] A latex agglutination test for the detection of Echinococcus multilocularis coproantigen in the definitive hosts (2008)
  • Author(s): Nonaka N.
  • Journal Title: Veterinary Parasitology 152 Pages: 278-283
  • Peer Reviewed
• [Journal Article] Control trial against Echinococcus multilocularis by local residents (2007)
  • Author(s): Oku, Y., Iwaki, T., Ganzorig, S., Nonaka, N., Miyahara, T., Okazaki, K. andKamiya, M.
  • Journal Title: Japanese Journal of Veterinary Parasitology 5(2) Pages: 36-36
• [Journal Article] 北海道のエキノコックス感染源対策の試みと今後の住民参加 (2007)
  • Author(s): 奥祐三郎, 巌城隆, スミヤ・ガンゾリク, 野中成晃, 井上貴史, 宮原俊之, 岡崎克則and神谷正男
  • Journal Title: 獣医畜産新報 60(5) Pages: 376-378
• [Journal Article] The use of tetracycline in anthelmintic baits to assess baiting rate and drug efficacy against Echinococcus multilocularis in foxes (2007)
  • Author(s): Inoue, T., Nonaka, N., Kanai, Y., Iwaki, T., Kamiya, M. and Oku, Y.
  • Journal Title: Veterinary Parasitology 150(1-2) Pages: 88-96
  • Peer Reviewed
• [Journal Article] Improvement of copro-DNA detection for diagnosis in prepatent period of Echinococcus multilocularis infection in dogs (2007)
  • Author(s): Inagakai, M., Nonaka, N., Inoue, T., Yagi, K., Katakura, K. and Oku, Y.
  • Journal Title: Japanese Journal of Veterinary Parasitology 5(2) Pages: 35-35
• [Journal Article] Improvement of copro-DNA detection for diagnosis in prepatent period of Echinococcus multilocularis infection in dogs (2007)
  • Author(s): Inagaki, M.
  • Journal Title: Japanese Journal of Veterinary Parasitology 5 Pages: 35-35
• [Journal Article] The use of tetracycline in anthelmintic baits to assess baiting rate and drug efficacy against Echinococcus multilocularis in foxes (2007)
  • Author(s): Inoue, T.
  • Journal Title: Veterinary Parasitology 150 Pages: 88-96
  • Peer Reviewed
• [Journal Article] 北海道のエキノコックス感染源対策の試みと今後の住民参加 (2007)
  • Author(s): 奥祐三郎
  • Journal Title: 獣医畜産新報 60 Pages: 376-378
• [Journal Article] Control trial against Echinococcus multilocularis by local residents (2007)
  • Author(s): Oku, Y.
  • Journal Title: Japanese Journal of Veterinary Parasitology 5 Pages: 36-36
• [Presentation] 犬のエキノコックス症診断のための糞便内抗原簡易検出キット「エキット」の評価 (2009)
  • Author(s): 野中成晃
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎市・ワールドコンベンションセンター
• [Presentation] 多包条虫の網羅的cDNAライブラリーのin silio解析、特にプロテアーゼ類について (2009)
  • Author(s): 奥祐三郎
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎市・ワールドコンベンションセンター
• [Presentation] ザンビアの牛から検出された単包条虫のgenotypeについて (2009)
  • Author(s): 中村星太
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎市・ワールドコンベンションセンター
• [Presentation] 犬用エキノコックス迅速診断キット「エキット」の信頼性と有用性について (2008)
  • Author(s): 野中成晃
  • Organizer: 北海道小動物獣医師会2008年次大会
  • Place of Presentation: 札幌市・ロイトンサッポロ
  • Year and Date: 2008-11-01
• [Presentation] 犬エキノコックス症の糞便内抗原簡易検出キット「エキット」の評価と感染源対策への応用 (2008)
  • Author(s): 神谷正男
  • Organizer: 第8回人と動物の共通感染症研究会学術集会
  • Place of Presentation: 東京都港区・北里大学薬学部コンベンションホール
  • Year and Date: 2008-11-01
• [Presentation] 犬用エキノコックス迅速診断キット「エキット」の信頼性と有用性について (2008)
  • Author(s): 野中成晃
  • Organizer: 北海道小動物獣医師会2008年次大会
  • Place of Presentation: 札幌市・ロイトンサツポロ
  • Year and Date: 2008-11-01
• [Presentation] ザンビアの牛から検出された単包条虫のgenotypeについて (2008)
  • Author(s): 中村星太
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎市・ワールドコンベンシヨンセンター
• [Presentation] 多包虫の網羅的cDNAライブラリーについて (2007)
  • Author(s): 奥祐三郎
  • Organizer: 第54回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 宮城県, 仙台市・宮城大学
  • Year and Date: 2007-09-21
• [Presentation] ザンビアにおける飼育犬の消化管内寄生虫調査 (2007)
  • Author(s): 野中成晃
  • Organizer: 第54回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 宮城県, 仙台市・宮城大学
  • Year and Date: 2007-09-21
• [Presentation] ザンビアにおける飼育犬の消化管内寄生虫調査 (2007)
  • Author(s): 野中成晃
  • Organizer: 第54回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 宮城県,仙台市・宮城大学
  • Year and Date: 2007-09-21
• [Presentation] 多包虫の網羅的cDNAライブラリーについて (2007)
  • Author(s): 奥祐三郎
  • Organizer: 第54回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
  • Place of Presentation: 宮城県,仙台市・宮城大学
  • Year and Date: 2007-09-21
• [Presentation] 多包虫の網羅的cDNAライブラリーについて (2007)
  • Author(s): 奥祐三郎
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 北海道, 江別市・酪農学園大学
  • Year and Date: 2007-09-02
• [Presentation] 本邦初報告となる飼育猫からの多包条虫卵の排泄 (2007)
  • Author(s): 野中成晃
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 北海道, 江別市・酪農学園大学
  • Year and Date: 2007-09-02
• [Presentation] 本邦初報告となる飼育猫からの多包条虫卵の排泄 (2007)
  • Author(s): 野中成晃
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 北海道,江別市・酪農学園大学
  • Year and Date: 2007-09-02
• [Presentation] 多包虫の網羅的cDNAライブラリーについて (2007)
  • Author(s): 奥祐三郎
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 北海道,江別市・酪農学園大学
  • Year and Date: 2007-09-02
• [Presentation] Development of a multiplex PCR to identify carnivore origins of feces for applying to epizootiological surveys of Echinococcus multilocularis in Hokkaido, Japan (2007)
  • Author(s): Nonaka, N.
  • Organizer: The 21st International Conference of the World Association for the Advancement of Veterinary Medicine
  • Place of Presentation: ベルギー・ゲント・国際会議センター
  • Year and Date: 2007-08-23
• [Presentation] Anthelmintic baiting to wild foxes by local residents for the control of alveoral echinococcosis in Hokkaido(Koshimizu and Kutchan), Japan. (2007)
  • Author(s): Oku, Y.
  • Organizer: The 21st International Conference of the World Association for the Advancement of Veterinary Medicine
  • Place of Presentation: ベルギー・ゲント・国際会議センター
  • Year and Date: 2007-08-23
• [Presentation] Anthelmintic baiting to wild foxes by local residents for the control of alveoral echinococcosis in Hokkaido(Koshimizu and Kutchan), Japan (2007)
  • Author(s): Oku, Y.
  • Organizer: The 21st International Conference of the World Association for the Advancement of Veterinary Medicine
  • Place of Presentation: ベルギー・ゲント・国際会議センター
  • Year and Date: 2007-08-23
• [Presentation] Prevalence study of Echinococcus granulosus and other parasites by three diagnostic techniques in rural farms in Uruguay (2007)
  • Author(s): Cabrera, P.
  • Organizer: The 22nd International Congress of Hydatidology & International Symposium of Zoonosis
  • Place of Presentation: ギリシャ・アテネ・ヒルトンホテル