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Diagnosis for Echinococcus definitive hosts in the early phase of infection and reconsideration of criteria for canine infection

Research Project

Project/Area Number 19580353
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionUniversity of Miyazaki (2008)
Hokkaido University (2007)

Principal Investigator

NONAKA Nariaki  University of Miyazaki, 農学部獣医学科, 准教授 (50281853)

Co-Investigator(Kenkyū-buntansha) KATAKURA Ken  北海道大学, 大学院・獣医学研究科, 教授 (10130155)
OKU Yuzaburo  北海道大学, 大学院・獣医学研究科, 准教授 (60133716)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords人畜共通感染症 / エキノコックス / 犬 / PCR / 診断 / 多包条虫 / 終宿主 / DNA / COI
Research Abstract

エキノコックス終宿主動物の感染早期(感染後約4週間の虫卵排出前期)において宿主糞便中に排出されるエキノコックスDNAのPCR検出法を開発した。高感度プライマーの設計, PCR粗阻害物質除去などDNA抽出法や反応条散発的であったが, 宿主を駆虫して得られる駆虫後糞便を検査することによりDNA検出率件を最適化し, その信頼性を検証したところ, 感染早期ではDNA検出率は26~73%とは100%となった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (45 results)

All 2009 2008 2007

All Journal Article (26 results) (of which Peer Reviewed: 14 results) Presentation (19 results)

  • [Journal Article] A vague understanding of the biology and epidemiology of echinococcosis by dog owners in Hokkaido, an endemic island for Echinococcu smultilocularis in Japan2009

    • Author(s)
      Nonaka, N., Kamiya, M. and Oku, Y.
    • Journal Title

      Journal of Veterinary Medical Science 71(1)

      Pages: 105-107

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Echinococcus multilocularis infection in pet dogs in Japan2009

    • Author(s)
      Nonaka, N., Kamiya, M., Kobayashi, F., Ganzorig, S., Ando, S., Yagi, K., Iwaki, T., Inoue, T. and Oku, Y.
    • Journal Title

      Vector-borne and Zoonotic Diseases 9(2)

      Pages: 201-205

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring of environmental contamination by Echinococcus multilocularis in an urban fringe forest part in Hokkaido, Japan2009

    • Author(s)
      Lagapa, J. T., Oku, Y., Kaneko, M., Ganzorig, S., Ono, T., Nonaka, N.,Kobayashi, F. and Kamiya, M.
    • Journal Title

      Environmental Health and Preventive Medicine 118(4)

      Pages: 576-582

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A vague understanding of the biology and epidemiology of echinococcosis by dog owners in Hokkaido, an endemic island for Echinococcus multilocularis in Japan2009

    • Author(s)
      Nonaka N.
    • Journal Title

      Journal of Veterinary Medical Science 71

      Pages: 105-107

    • NAID

      110007055397

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Echinococcus multilocularis infection in pet dogs in Japan2009

    • Author(s)
      Nonaka N.
    • Journal Title

      Vector-borne and Zoonotic Diseases 9

      Pages: 201-205

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] On a comprehensive full-length cDNA library of larval Echinococcus multilocularis2008

    • Author(s)
      Oku, Y., Nonaka, N., Matsumoto, J., Arumua, M. T., Sugimoto, C., Watanabe, J., Toyoda, A. and Kamiya, M.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 6(2)

      Pages: 80-80

    • Related Report
      2008 Final Research Report
  • [Journal Article] A first record of a cat excreting Echinococcus multilocularis eggs in Japan2008

    • Author(s)
      Nonaka, N., Hirokawa, H., Inoue, T.,Nakao, R., Ganzorig, S., Kobayashi, F.,Kamiya, M. and Oku, Y.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 6(2)

      Pages: 79-79

    • Related Report
      2008 Final Research Report
  • [Journal Article] Molecular mechanisms involved in the growth and development of Echinococcus multilocularis metacestodes2008

    • Author(s)
      Matsumoto, J., Nonaka, N., Katakura, K.and Oku, Y.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 6(1)

      Pages: 7-12

    • Related Report
      2008 Final Research Report
  • [Journal Article] Anaerobic NADH-fumarate reductase system is predominant in the respiratory chain of Echinococcus multilocularis, providing a novel target for the chemotherapy of alveolar echinococcosis2008

    • Author(s)
      Matsumoto, J., Sakamoto, K., Shinjyo,N., Kido, Y., Yamamoto, N., Yagi, K.,Miyoshi, H., Nonaka, N., Katakura, K., Kita, K. and Oku, Y.
    • Journal Title

      Antimicrobial Agents and Chemotherapy 52(1)

      Pages: 164-170

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The first instance of a cat excreting Echinococcus multilocularis eggs in Japan2008

    • Author(s)
      Nonaka, N., Hirokawa, H., Inoue, T.,Nakao, R., Ganzorig, S., Kobayashi, F., Inagaki, M., Egoshi, K., Kamiya, M. and Oku, Y.
    • Journal Title

      Parasitology International 57(4)

      Pages: 519-520

    • NAID

      10025329678

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A latex agglutination test for the detection of Echinococcus multilocularis coproantigen in the definitive hosts2008

    • Author(s)
      Nonaka, N., Oka, M., Kamiya, M. and Oku, Y.
    • Journal Title

      Veterinary Parasitology 152(3-4)

      Pages: 278-283

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The first instance of a cat excreting Echinococcus multilocularis eggs in Japan2008

    • Author(s)
      Nonaka, N.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 57

      Pages: 519-520

    • NAID

      10025329678

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anaerobic NADH-fumarate reductase system is predominant in the respiratory chain of Echinococcus multilocularis, providing a novel target for the chemotherapy of alveolar echinococcosis2008

    • Author(s)
      Matsumoto, J.
    • Journal Title

      Antimicrobial Agents and Chemotherapy 52

      Pages: 164-170

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms involved in the growth and development of Echinococcus multilocularis metacestodes2008

    • Author(s)
      Matsumoto, J.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 6

      Pages: 7-12

    • Related Report
      2008 Annual Research Report
  • [Journal Article] A first record of a cat excreting Echinococcus multilocularis eggs in Japan2008

    • Author(s)
      Nonaka, N.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 6

      Pages: 79-79

    • Related Report
      2008 Annual Research Report
  • [Journal Article] On a comprehensive full-length cDNA library of larval Echinococcus multilocularis2008

    • Author(s)
      Oku, Y.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 6

      Pages: 80-80

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Taenia taeniaeformis : Fate and proliferation of mucosal cells during gastric hyperplasia in larvae infected rats2008

    • Author(s)
      Lagapa, J. T.
    • Journal Title

      Experimental Parasitology 118

      Pages: 576-582

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A latex agglutination test for the detection of Echinococcus multilocularis coproantigen in the definitive hosts2008

    • Author(s)
      Nonaka N.
    • Journal Title

      Veterinary Parasitology 152

      Pages: 278-283

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control trial against Echinococcus multilocularis by local residents2007

    • Author(s)
      Oku, Y., Iwaki, T., Ganzorig, S., Nonaka, N., Miyahara, T., Okazaki, K. andKamiya, M.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 5(2)

      Pages: 36-36

    • Related Report
      2008 Final Research Report
  • [Journal Article] 北海道のエキノコックス感染源対策の試みと今後の住民参加2007

    • Author(s)
      奥祐三郎, 巌城隆, スミヤ・ガンゾリク, 野中成晃, 井上貴史, 宮原俊之, 岡崎克則and神谷正男
    • Journal Title

      獣医畜産新報 60(5)

      Pages: 376-378

    • Related Report
      2008 Final Research Report
  • [Journal Article] The use of tetracycline in anthelmintic baits to assess baiting rate and drug efficacy against Echinococcus multilocularis in foxes2007

    • Author(s)
      Inoue, T., Nonaka, N., Kanai, Y., Iwaki, T., Kamiya, M. and Oku, Y.
    • Journal Title

      Veterinary Parasitology 150(1-2)

      Pages: 88-96

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of copro-DNA detection for diagnosis in prepatent period of Echinococcus multilocularis infection in dogs2007

    • Author(s)
      Inagakai, M., Nonaka, N., Inoue, T., Yagi, K., Katakura, K. and Oku, Y.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 5(2)

      Pages: 35-35

    • Related Report
      2008 Final Research Report
  • [Journal Article] Improvement of copro-DNA detection for diagnosis in prepatent period of Echinococcus multilocularis infection in dogs2007

    • Author(s)
      Inagaki, M.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 5

      Pages: 35-35

    • Related Report
      2007 Annual Research Report
  • [Journal Article] The use of tetracycline in anthelmintic baits to assess baiting rate and drug efficacy against Echinococcus multilocularis in foxes2007

    • Author(s)
      Inoue, T.
    • Journal Title

      Veterinary Parasitology 150

      Pages: 88-96

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 北海道のエキノコックス感染源対策の試みと今後の住民参加2007

    • Author(s)
      奥祐三郎
    • Journal Title

      獣医畜産新報 60

      Pages: 376-378

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Control trial against Echinococcus multilocularis by local residents2007

    • Author(s)
      Oku, Y.
    • Journal Title

      Japanese Journal of Veterinary Parasitology 5

      Pages: 36-36

    • Related Report
      2007 Annual Research Report
  • [Presentation] 犬のエキノコックス症診断のための糞便内抗原簡易検出キット「エキット」の評価2009

    • Author(s)
      野中成晃
    • Organizer
      第146回日本獣医学会学術集会
    • Place of Presentation
      宮崎市・ワールドコンベンションセンター
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 多包条虫の網羅的cDNAライブラリーのin silio解析、特にプロテアーゼ類について2009

    • Author(s)
      奥祐三郎
    • Organizer
      第146回日本獣医学会学術集会
    • Place of Presentation
      宮崎市・ワールドコンベンションセンター
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ザンビアの牛から検出された単包条虫のgenotypeについて2009

    • Author(s)
      中村星太
    • Organizer
      第146回日本獣医学会学術集会
    • Place of Presentation
      宮崎市・ワールドコンベンションセンター
    • Related Report
      2008 Final Research Report
  • [Presentation] 犬用エキノコックス迅速診断キット「エキット」の信頼性と有用性について2008

    • Author(s)
      野中成晃
    • Organizer
      北海道小動物獣医師会2008年次大会
    • Place of Presentation
      札幌市・ロイトンサッポロ
    • Year and Date
      2008-11-01
    • Related Report
      2008 Final Research Report
  • [Presentation] 犬エキノコックス症の糞便内抗原簡易検出キット「エキット」の評価と感染源対策への応用2008

    • Author(s)
      神谷正男
    • Organizer
      第8回人と動物の共通感染症研究会学術集会
    • Place of Presentation
      東京都港区・北里大学薬学部コンベンションホール
    • Year and Date
      2008-11-01
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] 犬用エキノコックス迅速診断キット「エキット」の信頼性と有用性について2008

    • Author(s)
      野中成晃
    • Organizer
      北海道小動物獣医師会2008年次大会
    • Place of Presentation
      札幌市・ロイトンサツポロ
    • Year and Date
      2008-11-01
    • Related Report
      2008 Annual Research Report
  • [Presentation] ザンビアの牛から検出された単包条虫のgenotypeについて2008

    • Author(s)
      中村星太
    • Organizer
      第146回日本獣医学会学術集会
    • Place of Presentation
      宮崎市・ワールドコンベンシヨンセンター
    • Related Report
      2008 Annual Research Report
  • [Presentation] 多包虫の網羅的cDNAライブラリーについて2007

    • Author(s)
      奥祐三郎
    • Organizer
      第54回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
    • Place of Presentation
      宮城県, 仙台市・宮城大学
    • Year and Date
      2007-09-21
    • Related Report
      2008 Final Research Report
  • [Presentation] ザンビアにおける飼育犬の消化管内寄生虫調査2007

    • Author(s)
      野中成晃
    • Organizer
      第54回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
    • Place of Presentation
      宮城県, 仙台市・宮城大学
    • Year and Date
      2007-09-21
    • Related Report
      2008 Final Research Report
  • [Presentation] ザンビアにおける飼育犬の消化管内寄生虫調査2007

    • Author(s)
      野中成晃
    • Organizer
      第54回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
    • Place of Presentation
      宮城県,仙台市・宮城大学
    • Year and Date
      2007-09-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] 多包虫の網羅的cDNAライブラリーについて2007

    • Author(s)
      奥祐三郎
    • Organizer
      第54回日本寄生虫学会・日本衛生動物学会北日本支部合同大会
    • Place of Presentation
      宮城県,仙台市・宮城大学
    • Year and Date
      2007-09-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] 多包虫の網羅的cDNAライブラリーについて2007

    • Author(s)
      奥祐三郎
    • Organizer
      第144回日本獣医学会
    • Place of Presentation
      北海道, 江別市・酪農学園大学
    • Year and Date
      2007-09-02
    • Related Report
      2008 Final Research Report
  • [Presentation] 本邦初報告となる飼育猫からの多包条虫卵の排泄2007

    • Author(s)
      野中成晃
    • Organizer
      第144回日本獣医学会
    • Place of Presentation
      北海道, 江別市・酪農学園大学
    • Year and Date
      2007-09-02
    • Related Report
      2008 Final Research Report
  • [Presentation] 本邦初報告となる飼育猫からの多包条虫卵の排泄2007

    • Author(s)
      野中成晃
    • Organizer
      第144回日本獣医学会
    • Place of Presentation
      北海道,江別市・酪農学園大学
    • Year and Date
      2007-09-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] 多包虫の網羅的cDNAライブラリーについて2007

    • Author(s)
      奥祐三郎
    • Organizer
      第144回日本獣医学会
    • Place of Presentation
      北海道,江別市・酪農学園大学
    • Year and Date
      2007-09-02
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of a multiplex PCR to identify carnivore origins of feces for applying to epizootiological surveys of Echinococcus multilocularis in Hokkaido, Japan2007

    • Author(s)
      Nonaka, N.
    • Organizer
      The 21st International Conference of the World Association for the Advancement of Veterinary Medicine
    • Place of Presentation
      ベルギー・ゲント・国際会議センター
    • Year and Date
      2007-08-23
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] Anthelmintic baiting to wild foxes by local residents for the control of alveoral echinococcosis in Hokkaido(Koshimizu and Kutchan), Japan.2007

    • Author(s)
      Oku, Y.
    • Organizer
      The 21st International Conference of the World Association for the Advancement of Veterinary Medicine
    • Place of Presentation
      ベルギー・ゲント・国際会議センター
    • Year and Date
      2007-08-23
    • Related Report
      2008 Final Research Report
  • [Presentation] Anthelmintic baiting to wild foxes by local residents for the control of alveoral echinococcosis in Hokkaido(Koshimizu and Kutchan), Japan2007

    • Author(s)
      Oku, Y.
    • Organizer
      The 21st International Conference of the World Association for the Advancement of Veterinary Medicine
    • Place of Presentation
      ベルギー・ゲント・国際会議センター
    • Year and Date
      2007-08-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] Prevalence study of Echinococcus granulosus and other parasites by three diagnostic techniques in rural farms in Uruguay2007

    • Author(s)
      Cabrera, P.
    • Organizer
      The 22nd International Congress of Hydatidology & International Symposium of Zoonosis
    • Place of Presentation
      ギリシャ・アテネ・ヒルトンホテル
    • Related Report
      2008 Final Research Report 2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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