Analysis of specific structure of Clostridium botulinum C・D mosaic neurotoxins and the development of their detection methods

• Project/Area Number: 19580360
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Osaka Prefecture University
• Principal Investigator: KOZAKI Shunji Osaka Prefecture University, 生命環境科学研究科, 教授 (10109895)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ボツリヌス症 / ボツリヌス毒素 / 受容体 / 診断法 / イムノクロマト / ボツリヌス菌 / 神経毒素 / ガングリオシド

Research Abstract

鳥類や家畜のボツリヌス症由来菌の産生する「C・Dモザイク」毒素の特徴について、毒性と抗原性状を典型的なC型およびD型神経毒素と比較することにより明らかにした。特に最近、本邦で散発的に発生している牛ボツリヌス症由来菌について疫学的解析を行うとともに毒素の免疫学的手法を用いた迅速な特異検出方法の確立を試みた。

Research Products

• [Journal Article] Identification of the receptor-binding sites in the carboxyl-terminal half of the heavy chain of botulinum neurotoxin types C and D (2008)
  • Author(s): Tsukamoto, K., Kozai, Y., Ihara, H., Kohda, T., Mukamoto, M., Tsuji, T. and Kozaki, S.
  • Journal Title: Microb Pathog. 44 Pages: 484-493
  • Peer Reviewed
• [Journal Article] A protein chip membrane-capture assay for botulinum neurotoxin activity. (2008)
  • Author(s): Marconi s, Ferracci G, Bert homieu M, Kozaki S, Miquel is R, Boucraut J, Seagar M, Leveque C.
  • Journal Title: Toxicol Appl Pharmacol. 233 Pages: 439-446
  • Peer Reviewed
• [Journal Article] Identification of the receptor-binding sites in the carboxyl-terminal half of the heavy chain of botuhnum neurotoxins types C and D (2008)
  • Author(s): Tsukamoto, K.
  • Journal Title: Microbial Pathogenesis (印刷中)
  • Peer Reviewed
• [Presentation] Characterization of Clostridium botulinum D/C mosaic neurotoxin associated with bovine botulism in Japan (2008)
  • Author(s): 中村佳司
  • Organizer: IBRCC2008
  • Place of Presentation: アメリカ
  • Year and Date: 2008-08-14
• [Presentation] Pathogenicity of botulinum C・D mosaic toxins in animals (2008)
  • Author(s): 小崎俊司
  • Organizer: TOXINS2008
  • Place of Presentation: イタリア
  • Year and Date: 2008-06-13
• [Presentation] Pathogenicity of botulinum C・D mosaic toxins in animals (2008)
  • Author(s): Shunji Kozaki
  • Organizer: TOXINS 2008 - Basic and Therapeutic Aspects of Botulinum and Tetanus Toxins
  • Place of Presentation: イタリア
• [Presentation] Characterization of Clostridium botulinum D/C mosaic neurotoxin associated with bovine botulism in Japan. (2008)
  • Author(s): Keiji Nakamura
  • Organizer: The 45 th Interagency Botulism Research Coodinating Committee Meeting
  • Place of Presentation: アメリカ
• [Presentation] 牛ボツリヌス症由来菌神経毒素に特異な抗原部位の検索と迅速診断法の確立 (2007)
  • Author(s): 幸田知子
  • Organizer: 日本細菌学会関西支部
  • Place of Presentation: 大阪府
  • Year and Date: 2007-11-10
• [Presentation] 牛ボツリヌス症分離菌株産生毒素の性状解析 (2007)
  • Author(s): 中村佳司
  • Organizer: 第54回毒素シンポジウム
  • Place of Presentation: 大阪府
  • Year and Date: 2007-09-06
• [Presentation] 牛ボツリヌス症由来菌株の毒素の性状と迅速診断法確立への試み (2007)
  • Author(s): 小崎俊司
  • Organizer: 第143回日本獣医学会学術総会
  • Place of Presentation: 茨城県
  • Year and Date: 2007-04-03
• [Presentation] ボツリヌスC,D型神経毒素と脂質分子の多様な結合性について (2007)
  • Author(s): 塚本 健太郎
  • Organizer: 第54回毒素シンポジウム
  • Place of Presentation: 大阪