Analysis of the mechanisms that control replication fidelity of plant RNA viruses-toward development of stable attenuated viruses

• Project/Area Number: 19589001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: The University of Tokyo
• Principal Investigator: KOMATSU Ken The University of Tokyo, 大学院・農学生命科学研究科, 特任研究員 (60451837)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: ウイルス / 複製精度 / リバビリン / 塩基配列

Research Abstract

植物RNAウイルスの複製精度の制御機構を解明するための基盤的知見を得るため、植物RNAウイルスの感染植物内でのゲノムの多様性を比較解析した。オオバコモザイクウイルス(PlAMV)、ダイコンひだ葉モザイクウイルス(RaMV)など数多くの植物RNAウイルスの全塩基配列を決定した結果、PlAMVの分離株間の多様性が大きいこと、RaMVの分節ゲノム間で多様性が異なることなどを見出した

Research Products

• [Journal Article] Variability in the level of RNA silencing suppression caused by triplegene block protein 1 (TGBp1) from variouspotexviruses during infection (2009)
  • Author(s): Senshu, H., Ozeki, J., Komatsu, K.,Hashimoto, M., Hatada, K., Aoyama, M.,Kagiwada, S., Yamaji, Y., Namba, S.
  • Journal Title: Journal of General Virology 90 Pages: 1014-1024
  • Peer Reviewed
• [Journal Article] Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBpl) from various potexviruses during infection. (2009)
  • Author(s): Senshu, H., Ozeki, J., Komatsu, K., Hashimoto, M., Hatada, K., Aoyama, M., Kagiwada, S., Yamaji, Y., Namba, S.
  • Journal Title: Journal of General Virology 90 Pages: 1014-1024
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequence ofasparagus virus 3 (2008)
  • Author(s): Hashimoto M, Ozeki J, Komatsu K, SenshuH, Kagiwada S, Mori T, Yamaji Y, Namba S.
  • Journal Title: Archives of Virology 153 Pages: 219-221
  • Peer Reviewed
• [Journal Article] Nucleotide sequence analysis of sevenJapanese isolates of Plantago asiaticamosaic virus (PlAMV) : a unique potexvirus with significantly high genomic and biological variability within thespecies (2008)
  • Author(s): Komatsu K, Yamaji Y, Ozeki J, HashimotoM, Kagiwada S, Takahashi S, Namba S.
  • Journal Title: Archives of Virology 153 Pages: 193-198
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequenceof a new double-stranded RNA virus from therice blast fungus, Magnaporthe oryzae (2008)
  • Author(s): Maejima K., Himeno M., Komatsu, K., Kakizawa S., Yamaji, Y., Hamamoto, H.,Namba, S.
  • Journal Title: Archives of Virology 153 Pages: 381-391
  • Peer Reviewed
• [Journal Article] Completenucleotide sequence of a Californiaisolate of Radish mosaic virus (2008)
  • Author(s): Komatsu, K., Hatada K., Hashimoto, M., Ozeki, J., Maejima, K., Kagiwada, S., Yamaji, Y., Namba, S.
  • Journal Title: Archives of Virology 153 Pages: 2167-2168
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequence of a new double-stranded RNA virus from the rice blastfungus, Magnaporthe oryzae. (2008)
  • Author(s): Maejima K., Himeno M., Komatsu, K., Kakizawa S., Yamaji, Y., Hamamoto, H., Namba, S.
  • Journal Title: Archives of Virology 153 Pages: 389-391
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequence of a California isolate of Radish mosaic virus. (2008)
  • Author(s): Komatsu, K., Hatada K., Hashimoto, M., Ozeki, J., Maejima, K., Kagiwada, S., Yamaji, Y., Namba, S.
  • Journal Title: Archives of Virology 153 Pages: 2167-2168
  • Peer Reviewed
• [Journal Article] Complete nucleotide sequence of asparagus virus 3. (2008)
  • Author(s): Hashimoto M, Ozeki J, Komatsu K, Senshu H, Kagiwada S, Mori T, Yamaji Y, Namba S.
  • Journal Title: Archives of Virology 153 Pages: 219-221
  • Peer Reviewed
• [Journal Article] Nucleotide sequence analysis of seven Japanese isolates of Plantago asiatica mosaic virus(PIAMV): a unique potexvirus with significantly high genomic and biological variability within the species. (2008)
  • Author(s): Komatsu K, Yamaji Y, Ozeki J, Hashimoto M, Kagiwada S, Takahashi S, Namba S.
  • Journal Title: Archives of Virology 153 Pages: 193-198
  • Peer Reviewed
• [Journal Article] Genome sequence of a Japanese isolate of Radish mosaic virus : the first complete nucleotide sequence ofa crucifer-infecting comovirus (2007)
  • Author(s): Komatsu K., Hashimoto M., Maejima K., Ozeki J., Kagiwada S., Takahashi S., Yamaji Y., Namba S.
  • Journal Title: Archives of Virology 152 Pages: 1501-1506
  • Peer Reviewed
• [Journal Article] Genome sequence of a Japanese isolate of Radish mosaic virus: the first complete nucleotide sequence of a crucifer-infecting comovirus. (2007)
  • Author(s): Komatsu K., Hashimoto M., Maejima K., Ozeki J., Kagiwada S., Takahashi S., Yamaji Y., Namba S.
  • Journal Title: Archives of Virology 152 Pages: 1501-1506
  • Peer Reviewed
• [Presentation] Radishmosaic virus (RaMV)カリフォルニア分離株のゲノムRNA全塩基配列の解析 (2009)
  • Author(s): 畑田康司・川西剛史・尾関丈二・小松健・橋本将典・山次康幸・難波成任
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形(既発表)
  • Year and Date: 2009-03-27
• [Presentation] オオバコモザイクウイルス(PlAMV)がコードするtriple gene block protein 1(TGBp1)によるRNA サイレンシング抑制機構の解析 (2009)
  • Author(s): 千秋博子・尾関丈二・川西剛史・姫野未紗子・小松健・山次康幸・難波成任.
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形(既発表)
  • Year and Date: 2009-03-27
• [Presentation] Analysis of the systemic necrosis on Nicotianabenthamiana induced by plantago asiaticamosaic virus infection (2008)
  • Author(s): K. Komatsu, M.Hashimoto, J. Ozeki, M. Aoyama, Y. Yamaji, S. Namba
  • Organizer: 第14回国際ウイルス学会
  • Place of Presentation: トルコ・イスタンブール(既発表)
  • Year and Date: 2008-08-13
• [Presentation] Analysis of the systemic necrosis on Nicotiana benthamiana induced by plantago asiaticamosaic virus infection (2008)
  • Author(s): K. Komatsu, M.Hashimoto, J. Ozeki, M. Aoyama, Y. Yamaji, S. Namba
  • Organizer: 第14回 国際ウイルス学会
  • Place of Presentation: トルコ・イスタンブール
  • Year and Date: 2008-08-13
• [Presentation] オオバコモザイクウイルス(PlAMV)の遺伝学的多様性 (2008)
  • Author(s): 小松健・尾関丈二・千秋博子・姫野未紗子・山次康幸・難波成任
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 松江(既発表)
  • Year and Date: 2008-04-27
• [Presentation] Potexvirus属ウイルスによるRNAサイレンシング抑制能の比較解析 (2008)
  • Author(s): 千秋博子・尾関丈二・橋本将典・小松健・青山倫子・山次康幸・難波成任
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 松江(既発表)
  • Year and Date: 2008-04-27
• [Presentation] 糸状菌に感染するTotivirus科ウイルスの分類について (2008)
  • Author(s): 姫野未紗子・前島健作・小松健・山次康幸・濱本宏・難波成任
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 松江(既発表)
  • Year and Date: 2008-04-27