Structural analysis of proteins in living cells by using a novel imaging technique

• Project/Area Number: 19590033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: MIURA Takashi Tohoku University, 大学院薬学研究科, 准教授 (30222318)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: イメージング / ラマン分光法 / 細胞 / タンパク質 / アミロイド / アルツハイマー病

Research Abstract

アミロイド線維に対して高い親和性を持つアゾ色素であるコンゴーレッドの共鳴ラマン散乱を用いて、タンパク質が細胞に結合している状態でアミロイド線維に変化する過程を追跡することが可能な新規手法を開発した。本手法を用いることにより、1個の細胞の膜表面でのアミロイド形成を観察することが初めて可能となった。アルツハイマー病などの発症メカニズム解明のために、本手法は有用かつユニークな情報を与えると期待される。

Research Products

• [Journal Article] Non-electrostatic binding and self-association of amyloid β-peptide on the surface of tightly packed phosphatidylcholine membranes (2008)
  • Author(s): M. Yoda, T. Miura and H. Takeuchi
  • Journal Title: Biochem. Biophys. Res. Commun 376 Pages: 56-59
  • Peer Reviewed
• [Journal Article] Evidence for the Cation-π Interaction between Cu_<2+> and Tryptophan (2008)
  • Author(s): H. Yorita, K. Otomo, H. Hiramatsu, A. Toyama, T. Miura and H. Takeuchi
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 15266-15267
  • Peer Reviewed
• [Journal Article] Evidence for the Cation-π Interaction between Cu^<2+> and Tryptophan (2008)
  • Author(s): H. Yorita, K. Otomo, H. Hiramatsu, A. Toyama. T. Miura and H. Takeuchi
  • Journal Title: J. Am. Chem. Soc 130 Pages: 15266-15267
  • Peer Reviewed
• [Journal Article] Resonance Raman spectrum of Congo red as a probe of fibril formation of amyloid β-peptide (2008)
  • Author(s): T. Miura, C. Tsutsumi, M. Sasaki and H. Takeuchi
  • Journal Title: Proceedings of the XXth International Conference on Raman Spectroscopy Pages: 130-131
• [Journal Article] Clustered negative charges on the lipid membrane surface induce β-sheet formation of prion protein fragment 106-126 (2007)
  • Author(s): Miura T., Yoda M., Takaku N., Hirose T., Takeuchi H.
  • Journal Title: Biochemistry 46 Pages: 11589-11597
  • Peer Reviewed
• [Presentation] Resonance Raman spectrum of Congo red as a probe of fibril formation of amyloid β-peptide (2008)
  • Author(s): T. Miura, C. Tsutsumi, M. Sasaki and H. Takeuchi
  • Organizer: XXth International Conference on Raman Spectroscopy
  • Place of Presentation: London
  • Year and Date: 2008-08-22
• [Presentation] Resonance Raman spectrum of Congo red as a probe of fibril formation of amyloid β-peptide (2008)
  • Author(s): T. Miura
  • Organizer: XXth International Conference on Raman Spectroscopy
  • Place of Presentation: ロンドン(英国)
  • Year and Date: 2008-08-22
• [Presentation] Congo red のラマン散乱をプローブとする細胞膜表面におけるアミロイド線維形成の追跡 (2008)
  • Author(s): 堤千裕, 三浦隆史, 竹内英夫
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
• [Presentation] Congo Redのラマン散乱をプローブとするアミロイド線維の検出 (2007)
  • Author(s): 堤千裕, 三浦隆史, 竹内英夫
  • Organizer: 第4回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2007-06-04