Development of oligoarginine-conjugated lipid vector for gene delivery

• Project/Area Number: 19590046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Hoshi University
• Principal Investigator: MAITANI Yoshie Hoshi University, 薬学部, 教授 (10231581)
• Co-Investigator(Kenkyū-buntansha): KAWAKAMI Hiroko 野口研究所, 研究部, 研究員 (40320254)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 遺伝子ベクター / オリゴアルギニン / 生体膜貫通ペプチド / リポソーム / 非エンドサイトーシス / ミセル / ラクトース修飾 / 遺伝子導入 / リボソーム

Research Abstract

本研究では、遺伝子導入効率を上昇させるために、核移行能がある新規ラクトース脂質を合成し、これまでのオリゴアルギニンPEG脂質に混合したナノ粒子を調製し、グライコフェクションによる遺伝子導入ベクターの開発を目的とする。自己凝集するオリゴアルギニンPEG脂質と、ラクトースPEG脂質をそれぞれデザインし、合成した。遺伝子とラクトース修飾オリゴアルギニンミセルベクターとの複合体の構造を物理化学的手法で調べ、培養細胞における遺伝子導入効率との関係も調べた。

Research Products

• [Journal Article] Decaarginine-PEG-liposome Enhanced Transfection Efficiency and Function of Arginine Length and PEG (2009)
  • Author(s): M. Furuhata, T. Izumisawa, H. Kawakami, K. Toma, Y. Hattori, Y. Maitani
  • Journal Title: Int J Pharm 37(1-2) Pages: 40-46
• [Journal Article] Calcium enhanced delivery of tetraarginine-PEG-lipid-coated DNA/protamine complexes (2009)
  • Author(s): T. Fujita, M. Furuhata, Y. Hattori, H. Kawakami, K. Toma, Y. Maitani
  • Journal Title: Int J Pharm 368 Pages: 186-192
• [Journal Article] Non-ionic surfactant modified cationic liposomes mediated gene transfection in vitro and in the mouse lung (2009)
  • Author(s): W. Ding, T. Izumisawa, Y. Hattori, X. Qi, D. Kitamoto, Y. Maitani
  • Journal Title: Biol. Pharm. Bull. 32(2) Pages: 311-315
  • NAID: 110007042144
• [Journal Article] Surface properties of lipoplexes modified with MEL-A and Tween 80 (2009)
  • Author(s): W. Ding, Y. Hattori, X. Qi, D. Kitamoto, Y. Maitani
  • Journal Title: Chem. Pharm. Bull. 57(2) Pages: 138-143
• [Journal Article] Decaarginine-PEG-liposome Enhanced Transfection Efficiency and Function of Arginine Length and PEG (2009)
  • Author(s): M. Furuhata, T. Izumisawa, H. Kawakami, K. Toma, Y. Hattori, Y. Maitani
  • Journal Title: Int J Pharm. 37 Pages: 40-46
  • Peer Reviewed
• [Journal Article] Calcium enhanced delivery of tetraarginine-PEG-lipid-coated DNA/protamine complexes (2009)
  • Author(s): T. Fujita, M. Furuhata. Y. Hattori, H. Kawakami, K. Toma, Y. Maitani
  • Journal Title: Int J Pharm 368 Pages: 186-192
  • Peer Reviewed
• [Journal Article] High gene delivery in tumor by intratumoral injection of tetraarginine-PEG lipid-coated protamine/DNA (2008)
  • Author(s): T. Fujita, M. Furuhata, Y. Hattori, H. Kawakami, K. Toma, Y. Maitani
  • Journal Title: J. Control. Release 129(2) Pages: 124-127
• [Journal Article] Novel oligoarginine-linked PEG-lipid micelles for gene delivery (2008)
  • Author(s): M. Furuhata, Y. Hattori and Y. Maitani
  • Journal Title: Proc. Hoshi Univ. 50 Pages: 21-32
  • NAID: 40016648030
• [Journal Article] High gene delivery in tumor by intratumoral injection of tetraarginine-PEG lipid-coated protamine/DNA (2008)
  • Author(s): T. Fujita, M. Furuhata, Y. Hattori, H. Kawakami, K. Toma, Y. Maitani
  • Journal Title: J. Control. Release 129 Pages: 124-127
  • Peer Reviewed
• [Journal Article] Highly efficient cationic hydroxyethylated cholesterol-based nanoparticle-mediated gene transfer in vivo and in vitro in prostate carcinoma PC-3 cells (2007)
  • Author(s): Y.Hattori, W.Ding, Y.Maitani
  • Journal Title: J Control Release 120 Pages: 122-130
  • Peer Reviewed
• [Journal Article] Long-circulating liposome-encapsulated gancicloVir enhallces the efficacy of HSV-TK suicide gene therapy (2007)
  • Author(s): E.Kajiwara, K.Kawano, Y.Hattori, M.Fukushma, K.Hayashi, Y.Maitani
  • Journal Title: J Control Release 120 Pages: 104-110
  • Peer Reviewed
• [Journal Article] Cationic liposome(DC-Chol/DOPE=1:2)and a modified ethanol injection method to prepare liposomes,increased gene expression (2007)
  • Author(s): Y.Maitani, S.Igarashi, M.Sato, Y.Hattori
  • Journal Title: Int.J.Pharm 342 Pages: 33-39
  • Peer Reviewed
• [Presentation] オリゴアルギニン脂質/DNA複合体の細胞内動態 (2008)
  • Author(s): 小林昇平、古幡昌彦、R. Danev、永山國昭, 戸澗一孔、服部喜之、米谷芳枝、原口徳子
  • Organizer: 遺伝子・デリバリー研究会第8回シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-09
• [Presentation] オリゴアルギニンPEG修飾リポソームによる細胞内取り込み経路および遺伝子発現 (2008)
  • Author(s): 古幡昌彦、川上宏子、戸澗一孔、米谷芳枝
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] MEL-A modified MHAPC-liposomes for gene delivery in vitro and in the lung (2008)
  • Author(s): 丁武孝、服部喜之、米谷芳枝
  • Organizer: 第10回応用薬理シンポジウム
  • Place of Presentation: 東京
• [Presentation] MEL-A and Tween 80 modified cationic liposomes for gene transfection in vitro and in the lung (2008)
  • Author(s): 丁武孝、泉澤友宏、服部喜之、米谷芳枝
  • Organizer: 11th LIPOSOME RESEARCH DAYS CONFERENCE
  • Place of Presentation: 横浜
• [Presentation] Liposomal Drug Delivery : An Update (2008)
  • Author(s): Y. Maitani
  • Organizer: 22nd Federation of Asian Pharmaceutical Association Congress
  • Place of Presentation: シンガポール
• [Presentation] オリゴアルギニンミセル/DNA複合体の構造と遺伝子導入効率 (2007)
  • Author(s): 古幡昌彦、川上宏子、戸澗一孔、服部喜之、米谷芳枝
  • Organizer: 日本薬剤学会第22年会
  • Place of Presentation: 埼玉
• [Presentation] オリゴアルギニン脂質ベクターによる遺伝子の細胞内移行促進 (2007)
  • Author(s): 古幡昌彦
  • Organizer: 日本薬剤学会第22年会
  • Place of Presentation: 埼玉
• [Presentation] オリゴアルギニンミセル/DNA複合体の構造と遺伝子導入効率 (2007)
  • Author(s): 古幡 昌彦、川上 宏子、服部 喜之、戸澗 一孔、米谷 芳枝
  • Organizer: 日本薬剤学会第22年会
  • Place of Presentation: 大宮
• [Book] Tumor-targeting non-Viral gene therapy for the treatment of oral cancer, Cancer of the Mouth Research Trends (2007)
  • Author(s): Y.Hattori, Y.Maitani
  • Publisher: Nova Science Pubhshers,Inc
• [Remarks]
  • URL: http://polaris.hoshi.ac.jp/kyoshitsu/souzai/index1.html