Regulation of cell division by tyrosine phosphorylation-mediated control of organelle dynamics

• Project/Area Number: 19590058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Chiba University
• Principal Investigator: NAKAYAMA Yuji Chiba University, 大学院・薬学研究院, 講師 (10280918)
• Co-Investigator(Kenkyū-buntansha): FUKUMOTO Yasunori 千葉大学, 大学院・薬学研究院, 助教 (10447310) ; YAMAGUCHI Naoto 千葉大学, 大学院・薬学研究院, 教授 (00166620)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞分裂 / チロシンリン酸化 / Src型チロシンキナーゼ / 細胞内オルガネラ

Research Abstract

Cell division is tightly regulated in order to prevent cancers, cancer progression and cell death. In this study, we showed that tyrosine phosphorylation signaling by Src-family tyrosine kinases are important for regulation of cell division through mediating organelle dynamics. Especially, involvement of ERK/MAPK and Rab11 were shown.

Research Products

• [Journal Article] Dynamin participates in the maintenance of anterior polarity in the C. elegans embryo (2009)
  • Author(s): Nakayama Y, Shivas JM, Poole DS, Squirrell JM, Kulkoski JM, Schleede JB, Skop AR
  • Journal Title: Dev.Cell 16 Pages: 889-900
  • Peer Reviewed
• [Journal Article] Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway (2009)
  • Author(s): Nakayama Y, Igarashi A, Kikuchi I, Obata Y, Fukumoto Y, Yamaguchi N
  • Journal Title: Exp.Cell Res. 315 Pages: 2515-2528
  • Peer Reviewed
• [Journal Article] Differential trafficking of c-Src, Lyn, c-Yes, and Fyn is specified by the state of palmitoylation in the SH4 domain (2009)
  • Author(s): Sato I, Obata Y, Kasahara K, Nakayama Y, Fukumoto Y, Yamasaki T, Yokoyama KK, Saito T, Yamaguchi N
  • Journal Title: J.Cell Sci. 122 Pages: 965-975
  • Peer Reviewed
• [Journal Article] Requirement of the SH4 and tyrosine-kinase domains but not the kinase activity of Lyn for its biosynthetic targeting to caveolin-positive Golgi membranes (2009)
  • Author(s): Ikeda K, Nakayama Y, Ishii M, Obata Y, Kasahara K, Fukumoto Y, Yamaguchi N
  • Journal Title: Biochim.Biophys.Acta 1790 Pages: 1345-1352
  • Peer Reviewed
• [Journal Article] Nuclear localization of Src-family tyrosine kinases is required for growth factor-induced euchromatinization (2009)
  • Author(s): Takahashi A, Obata Y, Fukumoto Y, Nakayama Y, Kasahara K, Kuga T, Higashiyama Y, Saito T, Yokoyama KK, Yamaguchi N
  • Journal Title: Exp.Cell Res. 315 Pages: 1117-1141
  • Peer Reviewed
• [Journal Article] Induction of chromatin condensation by nuclear expression of a novel arginine-rich cationic protein genetically engineered from the enhanced green fluorescent protein (2009)
  • Author(s): Higashiyama Y, Takahashi A, Fukumoto Y, Nakayama Y, Yamaguchi N
  • Journal Title: Cytotechnology 60 Pages: 153-159
  • Peer Reviewed
• [Journal Article] Bleomycin-induced over-replication involves sustained inhibition of mitotic entry through the ATM/ATR pathway (2009)
  • Author(s): Yuji Nakayama
  • Journal Title: Experimental Cell Research 315 Pages: 2515-2528
  • Peer Reviewed
• [Journal Article] Dynamin participates in the maintenance of anterior polarity in the C.elegans embryo (2009)
  • Author(s): Yuji Nakayama
  • Journal Title: Developmental Cell 16 Pages: 889-900
  • Peer Reviewed
• [Journal Article] Differential trafficking of c-Src, Lyn, C-Yes, and Fyn is specified by the state of palmitoylation in the SH4 domain. (2009)
  • Author(s): Izumi Sato
  • Journal Title: Journal of Cell Science 122 Pages: 965-975
  • Peer Reviewed
• [Journal Article] Nuclear localization of Lyn tyrosine kinase mediated by inhibition of its kinase activity (2008)
  • Author(s): Ikeda K, Nakayama Y, Togashi Y, Obata Y, Kuga T, Kasahara K, Fukumoto Y, Yamaguchi N
  • Journal Title: Exp.Cell Res. 314 Pages: 3392-3404
  • Peer Reviewed
• [Journal Article] v-Src and c-Src, nonpalmitoylated Src-family kinases, induce perinuclear accumulation of lysosomes through Rab7 in a kinase activity-independent manner (2008)
  • Author(s): Kasahara K, Nakayama Y, Yamaguchi N
  • Journal Title: Cancer Lett. 262 Pages: 19-27
  • Peer Reviewed
• [Journal Article] Role of Src-family kinases in formation of the cortical actin cap at the dorsal cell surface (2008)
  • Author(s): Kuga T, Hoshino M, Nakayama Y, Kasahara K, Ikeda K, Obata Y, Takahashi A, Higashiyama Y, Fukumoto Y, Yamaguchi N
  • Journal Title: Exp.Cell Res. 314 Pages: 2040-2054
  • Peer Reviewed
• [Journal Article] Nuclear localization of magphinins, alternative splicing products of the human trophinin gene (2008)
  • Author(s): Aoyama J, Akazawa Y, Kasahara K, Higashiyama Y, Kikuchi I, Fukumoto Y, Saburi S, Nakayama Y, Fukuda M, Yamaguchi N
  • Journal Title: J.Cell.Biochem. 103 Pages: 765-777
  • Peer Reviewed
• [Journal Article] Nuclear localization of Lyn tyrosine kinase mediated by inhibition of its kinase activity. (2008)
  • Author(s): Kikuko Ikeda
  • Journal Title: Experimental Cell Research 314 Pages: 3392-3404
  • Peer Reviewed
• [Journal Article] v-Src and c-Src, nonpalmitoylated Src-family kinases, induce perinuclear accumulation of lysosomes through Rab7 in a kinaza activity-independent manner. (2008)
  • Author(s): kousuke Kasahara
  • Journal Title: Cancer letters 262 Pages: 19-27
  • Peer Reviewed
• [Journal Article] Separation of a disulfide-linked phosphoprotein by diagonal SDS-PAGE with optimized gel crosslinking (2007)
  • Author(s): Kuga T, Nakayama Y, Iwamatsu A, Fukumoto Y, Yokomori K, Yamaguchi N
  • Journal Title: Anal.Biochem. 370 Pages: 252-254
  • Peer Reviewed
• [Journal Article] Rapid trafficking of c-Src, a non-palmitoylated Src-family kinase, between the plasma membrane and late endosomes/lysosomes (2007)
  • Author(s): Kasahara K, Nakayama Y, Kihara A, Matsuda D, Ikeda K, Kuga T, Fukumoto Y, Igarashi Y, Yamaguchi N
  • Journal Title: Exp.Cell Res. 313 Pages: 2651-2666
  • Peer Reviewed
• [Journal Article] Src signaling regulates completion of abscission in cytokinesis through ERK/MAPK activation at the midbody (2007)
  • Author(s): Kasahara K, Nakayama Y, Nakazato Y, Ikeda K, Kuga T, Yamaguchi N
  • Journal Title: J.Biol.Chem. 282 Pages: 5327-5339
  • Peer Reviewed
• [Journal Article] Role of Src-family kinases in formation and trafficking of macropinosomes (2007)
  • Author(s): Kasahara K, Nakayama Y, Sato I, Ikeda K, Hoshino M, Endo T, Yamaguchi N
  • Journal Title: J.Cell.Physiol. 211 Pages: 220-232
  • Peer Reviewed
• [Journal Article] Differential mitotic activation of endogenous c-Src, c-Yes, and Lyn in HeLa cells (2007)
  • Author(s): Kuga T, Nakayama Y, Hoshino M, Higashiyama Y, Obata Y, Matsuda D, Kasahara K, Fukumoto Y, Yamaguchi N
  • Journal Title: Arch.Biochem.Biophys. 466 Pages: 116-124
  • Peer Reviewed
• [Journal Article] Src signaling regulates completion of abscission in cytokinesis through ERK/MAPK activation at the midbody (2007)
  • Author(s): Kousuke Kasahara
  • Journal Title: J. Biol. Chem 282 Pages: 5327-5339
  • Peer Reviewed
• [Journal Article] Differential mitotic activation of endogenous c-Src, c-Yes, and Lyn in HeLa cells (2007)
  • Author(s): Takahisa Kuga
  • Journal Title: Arch. Biochem. Biophys 466 Pages: 116-124
  • Peer Reviewed
• [Journal Article] Rapid traffcking of c-Src, a non-palmitoylated Src-family kinase, between the plasma membrane and late endosomes/lysosomes (2007)
  • Author(s): Kousuke Kasahara
  • Journal Title: Exp. Cell Res 313 Pages: 2651-2666
  • Peer Reviewed
• [Journal Article] A decrease in cyclin B1 levels leads to polyploidization in DNA-damage induced senescence
  • Author(s): Kikuchi I, Nakayama Y, Morinaga T, Fukumoto Y, Yamaguchi N
  • Journal Title: Cell Biology International (In press)
  • Peer Reviewed
• [Journal Article] Cost-effective gene transfection by DNA compaction at pH 4.0 using acidified, long shelf-life polyethylenimine
  • Author(s): Fukumoto Y, Obata Y, Ishibashi K, Tamura N, Kikuchi I, Aoyama K, Hattori Y, Tsuda K, Nakayama Y, Yamaguchi N
  • Journal Title: Cytotechnology (In press)
  • Peer Reviewed
• [Journal Article] A decrease in cyclin B1 levels leads to polyploidization in DNA-damage induced senescence.
  • Author(s): Ikue Kikuchi
  • Journal Title: Cell Biology International (In press) Pages: 2010-2010
  • Peer Reviewed
• [Presentation] 細胞分裂期スピンドル制御に関わるチロシンリン酸化シグナリング (2010)
  • Author(s): 中山祐治, 松井優紀, 津田邦彦, 福本泰典, 山口直人
  • Organizer: 第130年会日本薬学会
  • Place of Presentation: 岡山
• [Presentation] 癌遺伝子v-Src発現による細胞周期異常の誘導と癌化への関与 (2010)
  • Author(s): 添田修平, 中山祐治, 田村直樹, 松井優紀, 青木杏未, 福本泰典, 山口直人
  • Organizer: 第130年会日本薬学会
  • Place of Presentation: 岡山
• [Presentation] Src型チロシンキナーゼLynの細胞分裂期における局在 (2010)
  • Author(s): 津田邦彦, 福本泰典, 松井優紀, 服部泰之, 中山祐治, 山口直人
  • Organizer: 第130年会日本薬学会
  • Place of Presentation: 岡山
• [Presentation] 癌遺伝子v-Srcにより誘起される細胞分裂異常 (2010)
  • Author(s): 青木杏未, 中山祐治, 田村直樹, 添田修平, 松井優紀, 福本泰典, 山口直人
  • Organizer: 第130年会日本薬学会
  • Place of Presentation: 岡山
• [Presentation] Src型チロシンキナーゼによる細胞分裂期特異的なチロシンリン酸化の解析 (2009)
  • Author(s): 松井優紀
  • Organizer: 第129回日本薬学会年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-27
• [Presentation] 癌遺伝子v-Src発現による細胞周期への影響 (2009)
  • Author(s): 田村直樹, 中山祐治, 添田修平, 久保田翔, 松井優紀, 福本泰典, 山口直人
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ダブルタグLyn安定発現株を用いたLynのゴルジ局在化シグナリング機構の解析 (2009)
  • Author(s): 盛永敬郎, 福本泰典, 中山祐治, 岩間厚志, 山口直人
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Srcを介したチロシンリン酸化シグナリングによる細胞分裂制御 (2009)
  • Author(s): 松井優紀, 中山祐治, 青木杏未, 福本泰典, 山口直人
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 癌遺伝子v-Src発現による細胞周期異常の誘導 (2009)
  • Author(s): 添田修平, 中山祐治, 田村直樹, 松井優紀, 福本泰典, 山口直人
  • Organizer: 第53回日本薬学会関東支部大会
  • Place of Presentation: 埼玉
• [Presentation] Src型チロシンキナーゼによる細胞分裂特異的なチロシンリン酸化の解析 (2009)
  • Author(s): 松井優紀, 中山祐治, 福本泰典, 山口直人
  • Organizer: 第129回日本薬学会年会
  • Place of Presentation: 京都
• [Presentation] 細胞分裂後期におけるSrc型チロシンキナーゼによるチロシンリン酸化の役割 (2008)
  • Author(s): 松井優紀
  • Organizer: BMB2008 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 細胞分裂期の進行におけるSrc型チロシンキナーゼの関与 (2008)
  • Author(s): 松井優紀
  • Organizer: 第52回日本薬学会関東支部大会
  • Place of Presentation: 千葉
  • Year and Date: 2008-10-04
• [Presentation] Src型キナーゼによるアクチンキャップ形成制御と増殖シグナリング (2008)
  • Author(s): 久家 貴寿
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-28
• [Presentation] 細胞分裂後期におけるSrc型チロシンキナーゼによるチロシンリン酸化の役割 (2008)
  • Author(s): 松井優紀, 中山祐治, 福本泰典, 山口直人
  • Organizer: BMB2008第31回日本分子生物学会年会-第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] v-Src発現によるリソソームの動態への影響 (2008)
  • Author(s): 田村直樹, 中山祐治, 菊地郁江, 松井優紀, 福本泰典, 山口直人
  • Organizer: BMB2008第31回日本分子生物学会年会-第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 細胞分裂の進行におけるSrc型チロシンキナーゼの関与 (2008)
  • Author(s): 松井優紀, 中山祐治, 福本泰典, 山口直人
  • Organizer: 第52回日本薬学会関東支部大会
  • Place of Presentation: 千葉
• [Presentation] 癌遺伝子v-Srcの細胞内オルガネラでの発現解析 (2008)
  • Author(s): 田村直樹, 中山祐治, 菊地郁江, 松井優紀, 福本泰典, 山口直人
  • Organizer: 第52回日本薬学会関東支部大会
  • Place of Presentation: 千葉
• [Presentation] Src型キナーゼc-Src, c-Yes, Lynの細胞分裂期での異なった活性化様式 (2007)
  • Author(s): 久家貴寿, 中山祐治, 東山幸弘, 小幡裕希, 福本泰典, 山口直人
  • Organizer: 第51回日本薬学会関東支部大会
  • Place of Presentation: 東京
• [Remarks] ホームページ等
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• [Remarks]
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