Analysis of regulation mechanism of expression and activity of target molecule (C/EBPα) for therapy of leukemia.

• Project/Area Number: 19590082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Tokyo University of Science
• Principal Investigator: SHIMIZU Takahisa Tokyo University of Science, 総合研究機構, 講師 (30287479)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 発現制御 / 白血病 / レチノイン酸 / GM-CSF

Research Abstract

白血病治療のための重要な標的因子であるC/EBPαの発現及び活性制御機構に関する研究を行った結果、C/EBPαはその発現及び修飾(SUMO化)制御や、STAT5との複合体形成を介して、標的遺伝子であるId1遺伝子の発現を誘導している可能性が示唆された。この作用は白血病細胞を分化誘導し、がん化形質抑制に関与しているので、これら因子の同定は白血病治療において有益な情報となることが期待できる。

Research Products

• [Journal Article] Synergistic induction of gene expression during the differentiation into mature macrophage in human myeloblastic leukemia cells treated with TPA and KH1060. (2009)
  • Author(s): Shimizu T, Kuromi A, Takeda K.
  • Journal Title: Leuk Res. 33 Pages: 803-9
  • Peer Reviewed
• [Journal Article] Synergistic induction of gene expression during the differentiation into mature macrophage in human myeloblastic leukemia cells treated with TPA and KH1060. (2009)
  • Author(s): Takahisa Shitnizu, et al.
  • Journal Title: Leukemia Research 33 Pages: 803-809
  • Peer Reviewed
• [Journal Article] The effects of nanoparticles on mouse testis Leydig cells in vitro. (2009)
  • Author(s): Tomoko Komatus, et al.
  • Journal Title: Toxicology in Vitro 22 Pages: 1825-1831
  • Peer Reviewed
• [Journal Article] The effects of nanoparticles on mouse testis Leydig cells in vitro. (2008)
  • Author(s): Komatsu T, Tabata M, Kubo-Irie M, Shimizu T, Suzuki K, Nihei Y, Takeda K.
  • Journal Title: Toxicol In Vitro. 22 Pages: 1825-31
  • Peer Reviewed
• [Presentation] Analysis of gene expression during KH1060 plus TPA-induced differentiation of human myeloblastic leukemia cells. (2008)
  • Author(s): 清水 貴壽、武田 健
  • Organizer: 第67回 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Analysis of gene expression during KH1060 plus TPA-induced differentiation of human myeloblastic leukemia cells. (2008)
  • Author(s): 清水 貴壽
  • Organizer: 第67回日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Involvement of the helix-loop-helix protein ID-1 in granulocytic differentiation of human myeloblastic leukemia cells. (2008)
  • Author(s): Takahisa Shimizu, Ken Takeda.
  • Organizer: 20^<th> EORTC-NCI-AACR Symposium on Molecular Targets and Cancer Therapeutics
  • Place of Presentation: ジュネーブ(スイス)
  • Year and Date: 2008-10-24
• [Presentation] The expression analysis of histone-modifing enzymes during the differentiation of human myeloblastic leukemia cells. (2007)
  • Author(s): 清水 貴壽、武田 健
  • Organizer: 第66回 日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] The expression analysis of histone-modifing enzymes during the differentiation of human myeloblastic leukemia cells. (2007)
  • Author(s): 清水 貴壽
  • Organizer: 第 66 回日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04