Analysis of the mechanisms by which cyclin D1 controls hexokinase 2 function in breast cancer

• Project/Area Number: 19590087
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Nigata University of Phermacy and Applied Life Sciences
• Principal Investigator: SAKAMAKI Toshiyuki Nigata University of Phermacy and Applied Life Sciences, 薬学部, 准教授 (00445892)
• Co-Investigator(Kenkyū-buntansha): SATO Koji 新潟薬科大学, 薬学部, 助教 (10445893)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 遺伝子 / 癌 / 細胞・組織 / シグナル伝達 / 蛋白質

Research Abstract

ヒト乳腺上皮細胞株MCF10AにCyclin D1及びHexokinase 2を過剰発現させた安定発現株の樹立に成功した。また、ヒト乳癌細胞株MDA-MB-453及びSKBR3 にCyclin D1に対するsiRNAに導入し、安定発現株を樹立した。上記の安定発現株を用いた解析の結果、Cyclin D1がDNAメチル基転移酵素の発現に影響を及ぼす可能性が示唆され、さらに、Hexokinase 2を含む複数の遺伝子のプロモーターのメチル化状況の網羅的な解析を行った。

Research Products

• [Journal Article] ErbB2 Induces Notch1 Activity and Function in Breast Cancer Cells (2008)
  • Author(s): Lindsay J, Sakamaki T, Pestell RG.(他16名)
  • Journal Title: Clinical and Translational Science Vol.1 Pages: 107-115
  • Peer Reviewed
• [Journal Article] Musashi1 modulates mammary progenitor cell expansion through proliferin-mediated activation of the Wnt and Notch pathways (2008)
  • Author(s): Wang XY, Yin Y, Yuan H, Sakamaki T, Okano H, Glazer RI.
  • Journal Title: Molecular and Cellular Biology Vol.28 Pages: 3589-3599
  • Peer Reviewed
• [Journal Article] Musashil Modulates Mammary Progenitor Cell Expansion through Proliferin-Mediated Activation of the Wnt and Notch Pathways. (2008)
  • Author(s): Xiao-Yang Wang
  • Journal Title: Molecular and Cellular Biology 28 Pages: 3589-3599
  • Peer Reviewed
• [Journal Article] ErbB2 Induces Notchl Activity and Function in Breast Cancer Cells. (2008)
  • Author(s): Jaime Lindsay
  • Journal Title: Clinical and Translational Science 1 Pages: 107-115
  • Peer Reviewed
• [Journal Article] Menatetrenone, a vitamin K2 analogue, inhibits hepatocellular carcinoma cell growth by suppressing cyclin D1 expression through inhibition of nuclear factor kappaB activation (2007)
  • Author(s): Ozaki I, Sakamaki T, Yamamoto K.(他6名)
  • Journal Title: Clinical Cancer Research Vol.13 Pages: 2236-2245
  • Peer Reviewed
• [Journal Article] Menatetrenone, a vitamin K2 analogue, inhibits hepatocellular carcinoma cell growth by suppressing cyclin D1 expression through inhibition of nuclear factor kappaB activation. (2007)
  • Author(s): Iwata Ozaki
  • Journal Title: Clinical Cancer Research 13 Pages: 2236-2245
  • Peer Reviewed
• [Presentation] 乳癌におけるサイクリンD1の機能解析 (2007)
  • Author(s): 酒巻利行
  • Organizer: 第48回新潟生化学懇話会
  • Place of Presentation: 新潟大学
  • Year and Date: 2007-10-13
• [Presentation] 乳癌におけるサイクリンD1の機能解析 (2007)
  • Author(s): 酒巻 利行
  • Organizer: 第48回 新潟生化学懇話会
  • Place of Presentation: 新潟大学大学院自然科学研究科管理棟大会議室
  • Year and Date: 2007-10-13