Analysis of the mechanisms by which cyclin D1 controls hexokinase 2 function in breast cancer
| Project/Area Number |
19590087
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
SAKAMAKI Toshiyuki Nigata University of Phermacy and Applied Life Sciences, 薬学部, 准教授 (00445892)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Koji 新潟薬科大学, 薬学部, 助教 (10445893)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 遺伝子 / 癌 / 細胞・組織 / シグナル伝達 / 蛋白質 |
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| Research Abstract |
ヒト乳腺上皮細胞株MCF10AにCyclin D1及びHexokinase 2を過剰発現させた安定発現株の樹立に成功した。また、ヒト乳癌細胞株MDA-MB-453及びSKBR3 にCyclin D1に対するsiRNAに導入し、安定発現株を樹立した。上記の安定発現株を用いた解析の結果、Cyclin D1がDNAメチル基転移酵素の発現に影響を及ぼす可能性が示唆され、さらに、Hexokinase 2を含む複数の遺伝子のプロモーターのメチル化状況の網羅的な解析を行った。
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Report
(3 results)
Research Products
(8 results)
