Novel Molecular Targeted Therapy for Alopecia/Hairloss via PKCeta

• Project/Area Number: 19590107
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Drug development chemistry
• Research Institution: Showa University
• Principal Investigator: OHBA Motoi Showa University, 腫瘍分子生物学研究所, 講師 (70297018)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: PKC / 分子標的薬 / 再生 / 毛髪 / 再生医療

Research Abstract

PKCηを分子標的とした毛髪再生技術の確立を目指した。第1に、既知のPKCη活性化脂質でであるコレステロール硫酸のナノ粒子化による発毛誘導実験を行った。第2に、新規PKCη活性化剤・転写誘導剤の探索をFRET法を原理としたシステム、PKCηプロモーターを使用したレポーターシステムを用い行った。さらに、PKCηトランスジェニックマウスの解析を通じて、PKCηによる発毛誘導機構の解析を行った。

Research Products

• [Journal Article] Functional Characterization of IL-17F as a Selective Neutrophil Attractant in Psoriasis (2009)
  • Author(s): Watanabe H, , Ohba M, Hizawa N, Adachi M, Huang SK, Iijima M. (他6名)
  • Journal Title: J. Invest. Dermatol. 129(3) Pages: 650-6
  • Peer Reviewed
• [Journal Article] Functional Characterization of IL-17F as a Selective Neutrophil Attractant in Psoriasis (2009)
  • Author(s): Watanabe H.
  • Journal Title: J Invest Dermatol. 129(3) Pages: 650-656
  • Peer Reviewed
• [Journal Article] Protein Kinase Cα and ζ Differentially Regulate Death-Inducing Signaling Complex Formation in Cigarette Smoke Extract-Induced Apoptosis (2008)
  • Author(s): Park JW, Kim HP, Lee SJ, Wang X, Wang Y, Ifedigbo E, Watkins SC, Ohba M, Ryter S.W., Vyas Y.M., Choi A.M.
  • Journal Title: J. Immunol. 180(7) Pages: 4668-78
  • Peer Reviewed
• [Journal Article] Protein Kinase Cα and ζ Differentially Regulate Death-Inducing Signaling Complex Formation in Cigarette Smoke Extract-Induced Apoptosis (2008)
  • Author(s): Park J., W., et. al.
  • Journal Title: J.Immunology 180 Pages: 4668-4678
  • Peer Reviewed
• [Journal Article] A protein kinase Cε-anti-apoptotic kinase signaling complex protects human vascular endothelial cells against apoptosis through induction of Bcl-2. (2007)
  • Author(s): Steinberg R, Harari OA, Lidington EA, Boyle JJ, Nohadani M, Samarel AM, Ohba M, Haskard DO, Mason JC.
  • Journal Title: J. J. Biol. Chem. 282(44) Pages: 32288-97
  • Peer Reviewed
• [Journal Article] Activation of protein kinaseCζ mediates luteinizing hormone-or forskolin-induced NGFI-B expression in preovulatory granulosa cells of rat ovary (2007)
  • Author(s): Park J.I., Kim S.G., Chun J.S., Seo Y.M., Jeon M.J., Ohba M., Kim H.J., Chun S.Y.
  • Journal Title: Mol. Cell Endocrinol. 270(1-2) Pages: 79-86
  • Peer Reviewed
• [Journal Article] Activation of protein・kinase Cζmediates luteinizing hormone-or forskolin-induced NGFI-B expression in preovulatory granulosa cells of rat ovary (2007)
  • Author(s): Park J., I, et. al.
  • Journal Title: Mol.Cell Endocrinol. 270 Pages: 79-86
  • Peer Reviewed
• [Presentation] 毛包上皮幹細胞の永代培養法と分化誘導法の確立 (2009)
  • Author(s): 大場 基
  • Organizer: 昭和大学共同研究成果発表会
  • Place of Presentation: 東京都
  • Year and Date: 2009-03-21
• [Presentation] PKCηを標的分子とした毛髪再生法の開発 (2008)
  • Author(s): 大場基
  • Organizer: 昭和大学共同研究 研究成果発表会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-01
• [Presentation] PKCηを標的分子とした毛髪再生法の開発 (2008)
  • Author(s): 大場 基
  • Organizer: 昭和大学共同研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-01
• [Presentation] Relation between MET and EGFR expression in acquired gefetinib-resistant non small lung cancer (2008)
  • Author(s): Kanome. T.
  • Organizer: AACR Annual Meeting 2008
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Involvement of PKCeta in growth and differentiation of epithelial cells and innate immune response (2008)
  • Author(s): 大場基
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Involvement of PKCeta in growth and differentiation of epithelial cells and innate immune response (2008)
  • Author(s): 大場 基
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神戸市
• [Book] 改訂培養細胞実験ハンドブック (2009)
  • Publisher: 羊土社
• [Book] 改訂 培養細胞実験ハンドブック (2009)
  • Author(s): 大場 基
  • Total Pages: 329
  • Publisher: 羊土社
• [Patent(Industrial Property Rights)] 毛髪再生方法 (2008)
  • Inventor(s): 大場基、黒木登志夫
  • Industrial Property Rights Holder: 昭和大学、(株)バイオマスター
  • Industrial Property Number: 2004-264736
  • Filing Date: 2008